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The map label for this gene is miaB [H]

Identifier: 238028582

GI number: 238028582

Start: 3460609

End: 3461982

Strand: Direct

Name: miaB [H]

Synonym: bglu_1g30460

Alternate gene names: 238028582

Gene position: 3460609-3461982 (Clockwise)

Preceding gene: 238028580

Following gene: 238028583

Centisome position: 88.59

GC content: 66.45

Gene sequence:

>1374_bases
ATGACGAAAAAAGTTTACGTAAAGACCTTCGGCTGCCAGATGAACGAGTACGACTCGGACAAGATGGTGGACGTGCTCAA
CGCCGCCGAAGGGCTCGAAAAGACCGACAGGCCCGAAGACGCGGACGTGATCCTGTTCAACACCTGCTCGGTGCGCGAGA
AGGCCCAGGAAAAGGTGTTCTCGGATCTCGGCCGGGTGCGCGAGCTGAAGGAAGCCAAGCCCGGGCTGATCATCGGCGTG
GGCGGCTGCGTGGCGAGCCAGGAAGGCGCGGCGATCGTCGCGCGCGCGCCCTACGTGGACCTCGTGTTCGGCCCGCAGAC
GCTGCACCGGCTGCCGAAGATGATCGACGCGCGCCGCGCGAGCGGCCGCTCGCAGGTCGACATCACGTTTCCCGAGATCG
AGAAGTTCGACCATCTGCCGCCCGCGCGCGTGGAGGGCCCGAGCGCGTTCGTCTCGATCATGGAAGGCTGCAGCAAGTAT
TGCAGCTACTGCGTGGTGCCCTACACGCGCGGCGACGAGGTCTCGCGCCCGCTCGACGACGTGCTGACCGAGATCGCCGG
GCTCGCCGACCAGGGCGTGCGCGAAGTCACGCTGCTGGGCCAGAACGTCAACGCCTACCGCGGCGCGCTGACGGCCGGCT
CGACCGAGATCGCCGATTTCGCCACGCTGATCGAGTACGTGGCCGACCTGCCCGGCATCGAGCGGATCCGCTACACCACC
TCGCATCCGAAGGAATTCACGCAGCGCCTGCTCGAGGTCTACGCGCGCGTGCCGAAGCTCGTCAACCATCTGCACCTGCC
GGTCCAGCACGGCTCGGACCGCGTGCTGATGGCGATGAAGCGCGGCTACACGGTGCTCGAATACAAGTCGCTGATCCGCA
AGCTGCGCGCGATCCGCCCCGGCCTGTCGCTGTCCACCGACATCATCGTCGGCTTCCCCGGCGAGACCGAGGCCGACTTC
GACAAGACCATGGCGCTCGTCCACGAGATGCGCTACGACACCAGCTTCTCGTTCATCTACAGCCCGCGCCCCGGCACGCC
GGCCGCCAACCTGCACGACGACACGCCGCGCGAGGTCAAGCTCAAACGCCTGCAACATCTGCAGGCCACCATCGAGGAGA
ACGTCGCGCATATCAGCGCGTCGATGGTCGGCGGGGTCGAGCGGATTCTCGTGGAAGGCCCGTCGCGCAAGGACCCGAAC
GAGCTGGCGGGACGCACCGAGAACAATCGCGTGGTCAACTTCCCGGCACCGCTGGCCTCGCACGCGCGGCTGATCGGCCA
GATGATCGACGTGCGGATCAACCATGCCTATCCGCATTCGCTGCGCGGCGAACTCGTGCTGGCCCACGACGACGCCAGCA
CGGCCAGCCACTGA

Upstream 100 bases:

>100_bases
GCGCGGGGCCGGCAGCGCAACCGGGCGCGGGCCGCCCGGACCGTCCCGATTCCCCCGCAACGACGCCCCCAGGTCAACCA
CTGCGAACGGCGAACCCCAG

Downstream 100 bases:

>100_bases
GCGCCCAACCGAAGGAAGCCGACGCAACCTTGAAAGCCACCCAAGCCCTGGAATTCACCGCGCCGCGTGACGACAACGCG
CGCCTCGCGAACCTCTGCGG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDRPEDADVILFNTCSVREKAQEKVFSDLGRVRELKEAKPGLIIGV
GGCVASQEGAAIVARAPYVDLVFGPQTLHRLPKMIDARRASGRSQVDITFPEIEKFDHLPPARVEGPSAFVSIMEGCSKY
CSYCVVPYTRGDEVSRPLDDVLTEIAGLADQGVREVTLLGQNVNAYRGALTAGSTEIADFATLIEYVADLPGIERIRYTT
SHPKEFTQRLLEVYARVPKLVNHLHLPVQHGSDRVLMAMKRGYTVLEYKSLIRKLRAIRPGLSLSTDIIVGFPGETEADF
DKTMALVHEMRYDTSFSFIYSPRPGTPAANLHDDTPREVKLKRLQHLQATIEENVAHISASMVGGVERILVEGPSRKDPN
ELAGRTENNRVVNFPAPLASHARLIGQMIDVRINHAYPHSLRGELVLAHDDASTASH

Sequences:

>Translated_457_residues
MTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDRPEDADVILFNTCSVREKAQEKVFSDLGRVRELKEAKPGLIIGV
GGCVASQEGAAIVARAPYVDLVFGPQTLHRLPKMIDARRASGRSQVDITFPEIEKFDHLPPARVEGPSAFVSIMEGCSKY
CSYCVVPYTRGDEVSRPLDDVLTEIAGLADQGVREVTLLGQNVNAYRGALTAGSTEIADFATLIEYVADLPGIERIRYTT
SHPKEFTQRLLEVYARVPKLVNHLHLPVQHGSDRVLMAMKRGYTVLEYKSLIRKLRAIRPGLSLSTDIIVGFPGETEADF
DKTMALVHEMRYDTSFSFIYSPRPGTPAANLHDDTPREVKLKRLQHLQATIEENVAHISASMVGGVERILVEGPSRKDPN
ELAGRTENNRVVNFPAPLASHARLIGQMIDVRINHAYPHSLRGELVLAHDDASTASH
>Mature_456_residues
TKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDRPEDADVILFNTCSVREKAQEKVFSDLGRVRELKEAKPGLIIGVG
GCVASQEGAAIVARAPYVDLVFGPQTLHRLPKMIDARRASGRSQVDITFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYC
SYCVVPYTRGDEVSRPLDDVLTEIAGLADQGVREVTLLGQNVNAYRGALTAGSTEIADFATLIEYVADLPGIERIRYTTS
HPKEFTQRLLEVYARVPKLVNHLHLPVQHGSDRVLMAMKRGYTVLEYKSLIRKLRAIRPGLSLSTDIIVGFPGETEADFD
KTMALVHEMRYDTSFSFIYSPRPGTPAANLHDDTPREVKLKRLQHLQATIEENVAHISASMVGGVERILVEGPSRKDPNE
LAGRTENNRVVNFPAPLASHARLIGQMIDVRINHAYPHSLRGELVLAHDDASTASH

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=37.2384937238494, Blast_Score=302, Evalue=4e-82,
Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=37.2384937238494, Blast_Score=301, Evalue=7e-82,
Organism=Homo sapiens, GI93277076, Length=457, Percent_Identity=27.7899343544858, Blast_Score=170, Evalue=2e-42,
Organism=Escherichia coli, GI1786882, Length=451, Percent_Identity=61.4190687361419, Blast_Score=564, Evalue=1e-162,
Organism=Escherichia coli, GI1787057, Length=461, Percent_Identity=29.0672451193059, Blast_Score=159, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17553146, Length=466, Percent_Identity=35.6223175965665, Blast_Score=281, Evalue=5e-76,
Organism=Caenorhabditis elegans, GI71996771, Length=300, Percent_Identity=29.3333333333333, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI21356207, Length=475, Percent_Identity=34.9473684210526, Blast_Score=288, Evalue=5e-78,
Organism=Drosophila melanogaster, GI19922432, Length=459, Percent_Identity=29.8474945533769, Blast_Score=160, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 50587; Mature: 50456

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDRPEDADVILFNTCSVREKAQEKVF
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHH
SDLGRVRELKEAKPGLIIGVGGCVASQEGAAIVARAPYVDLVFGPQTLHRLPKMIDARRA
HHHHHHHHHHHCCCCEEEEECCCEECCCCCEEEEECCEEEEEECHHHHHHHHHHHHHHHC
SGRSQVDITFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD
CCCCEEEEECCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHH
VLTEIAGLADQGVREVTLLGQNVNAYRGALTAGSTEIADFATLIEYVADLPGIERIRYTT
HHHHHHHHHHCCCEEEEEECCCCHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECC
SHPKEFTQRLLEVYARVPKLVNHLHLPVQHGSDRVLMAMKRGYTVLEYKSLIRKLRAIRP
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHCCCCHHHHHHHHHHHHHHCC
GLSLSTDIIVGFPGETEADFDKTMALVHEMRYDTSFSFIYSPRPGTPAANLHDDTPREVK
CCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHH
LKRLQHLQATIEENVAHISASMVGGVERILVEGPSRKDPNELAGRTENNRVVNFPAPLAS
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHEECCCCCCCCHHHCCCCCCCEEEECCCCHHH
HARLIGQMIDVRINHAYPHSLRGELVLAHDDASTASH
HHHHHHHHHHHEECCCCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
TKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDRPEDADVILFNTCSVREKAQEKVF
CCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHH
SDLGRVRELKEAKPGLIIGVGGCVASQEGAAIVARAPYVDLVFGPQTLHRLPKMIDARRA
HHHHHHHHHHHCCCCEEEEECCCEECCCCCEEEEECCEEEEEECHHHHHHHHHHHHHHHC
SGRSQVDITFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD
CCCCEEEEECCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHH
VLTEIAGLADQGVREVTLLGQNVNAYRGALTAGSTEIADFATLIEYVADLPGIERIRYTT
HHHHHHHHHHCCCEEEEEECCCCHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECC
SHPKEFTQRLLEVYARVPKLVNHLHLPVQHGSDRVLMAMKRGYTVLEYKSLIRKLRAIRP
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHCCCCHHHHHHHHHHHHHHCC
GLSLSTDIIVGFPGETEADFDKTMALVHEMRYDTSFSFIYSPRPGTPAANLHDDTPREVK
CCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHH
LKRLQHLQATIEENVAHISASMVGGVERILVEGPSRKDPNELAGRTENNRVVNFPAPLAS
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHEECCCCCCCCHHHCCCCCCCEEEECCCCHHH
HARLIGQMIDVRINHAYPHSLRGELVLAHDDASTASH
HHHHHHHHHHHEECCCCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA