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The map label for this gene is lpfB [H]

Identifier: 238027859

GI number: 238027859

Start: 2500681

End: 2501421

Strand: Direct

Name: lpfB [H]

Synonym: bglu_1g22900

Alternate gene names: 238027859

Gene position: 2500681-2501421 (Clockwise)

Preceding gene: 238027858

Following gene: 238027860

Centisome position: 64.01

GC content: 67.61

Gene sequence:

>741_bases
ATGACCGCCCCGCATGCCACACCGCGGCGCGCATCGGCTGCCGCAAGGCGCCTGCCGTATCGCGTCGCCGCCGTCGCGCT
GGCGGTGGCGGCGCTTCTGCCCTGGCAATCGGCCCATGCGGACGTCCGGCTCAGCAAGACCCGCGTGATCTATGCGGCCA
GCCATCCGGACGCGACGCTCACCGTGTCGAACGCCGGTACCGAGCCGGCCATCGTGCAGACCTGGATCGAAACGGCCGAC
GACAAGCTCGATACCCCGTTCTTCGCCGCGCCGCCCTTGTTCCGCCTCGATCCGGGCGATACCAACCTGATCCGCGTGAC
GCTGGTCGGACAGGCGCCGGACACGCGCGTCGAGCGTCTCTATTGGCTCAATGTGAAGGAGATCCCGCCAGCGCCGCAGG
CTTCCGCGGGCAATACGCTGCAATTCGCGATCCGCACGCGCATCAAGCTGCTGTACCGACCGCCCGGGCTCGACGGCGCA
TTCGACCTGCAACGCGACCTGAGGCTGAGCCGAGGCGGCGCATCGGTGGAAAACACCTCGCCGTATGTCGCCTCACTGTT
CGTCACCTGCCCCGAAGACGACCGACAGACATCCTTGACCGTCAATCCGCATGCTTCGTTGCCGATGCCCAGGATGTCCT
GCCCGCCGGCCGATGTCCGAATCCAATCGATCAACGACTACGGCGGCATCAGCGCGCCCTTCACGCCGCCGCTCGACGGC
CCGGTGTCCACGCCGCACTAG

Upstream 100 bases:

>100_bases
ATTCCTTTCGCCGCCCAATACACGTCTAACGGCGGCACCCCCGTTGCCGGCAAGGCAAACGCCATCGCGCAGTTCACGCT
TTCCTATCCATGAGTTCTAG

Downstream 100 bases:

>100_bases
GCTCGCGCTCGCCGTGTTGCTGTTCGCCACCGCCGGCACGGCCAGAAGCGGCGACGCACTGCGTTTCAACAACGACTTCC
TGACGCTGGACGGTGTGGCA

Product: pili assembly chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTAPHATPRRASAAARRLPYRVAAVALAVAALLPWQSAHADVRLSKTRVIYAASHPDATLTVSNAGTEPAIVQTWIETAD
DKLDTPFFAAPPLFRLDPGDTNLIRVTLVGQAPDTRVERLYWLNVKEIPPAPQASAGNTLQFAIRTRIKLLYRPPGLDGA
FDLQRDLRLSRGGASVENTSPYVASLFVTCPEDDRQTSLTVNPHASLPMPRMSCPPADVRIQSINDYGGISAPFTPPLDG
PVSTPH

Sequences:

>Translated_246_residues
MTAPHATPRRASAAARRLPYRVAAVALAVAALLPWQSAHADVRLSKTRVIYAASHPDATLTVSNAGTEPAIVQTWIETAD
DKLDTPFFAAPPLFRLDPGDTNLIRVTLVGQAPDTRVERLYWLNVKEIPPAPQASAGNTLQFAIRTRIKLLYRPPGLDGA
FDLQRDLRLSRGGASVENTSPYVASLFVTCPEDDRQTSLTVNPHASLPMPRMSCPPADVRIQSINDYGGISAPFTPPLDG
PVSTPH
>Mature_245_residues
TAPHATPRRASAAARRLPYRVAAVALAVAALLPWQSAHADVRLSKTRVIYAASHPDATLTVSNAGTEPAIVQTWIETADD
KLDTPFFAAPPLFRLDPGDTNLIRVTLVGQAPDTRVERLYWLNVKEIPPAPQASAGNTLQFAIRTRIKLLYRPPGLDGAF
DLQRDLRLSRGGASVENTSPYVASLFVTCPEDDRQTSLTVNPHASLPMPRMSCPPADVRIQSINDYGGISAPFTPPLDGP
VSTPH

Specific function: Required for the biogenesis of long polar fimbria; binds and interact with lpfA [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1789532, Length=226, Percent_Identity=31.858407079646, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1786333, Length=222, Percent_Identity=33.7837837837838, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790771, Length=239, Percent_Identity=30.9623430962343, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787171, Length=219, Percent_Identity=28.310502283105, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786743, Length=219, Percent_Identity=29.2237442922374, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788428, Length=137, Percent_Identity=31.3868613138686, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1786936, Length=208, Percent_Identity=27.8846153846154, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI87082203, Length=227, Percent_Identity=28.1938325991189, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788677, Length=200, Percent_Identity=28, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 26588; Mature: 26457

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPHATPRRASAAARRLPYRVAAVALAVAALLPWQSAHADVRLSKTRVIYAASHPDATL
CCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEECEEEEEEECCCCEEE
TVSNAGTEPAIVQTWIETADDKLDTPFFAAPPLFRLDPGDTNLIRVTLVGQAPDTRVERL
EEECCCCCCHHHHHHHHHCCCCCCCCEECCCCEEEECCCCCCEEEEEEECCCCCCCEEEE
YWLNVKEIPPAPQASAGNTLQFAIRTRIKLLYRPPGLDGAFDLQRDLRLSRGGASVENTS
EEECCCCCCCCCCCCCCCEEEEEEEEEEEEEECCCCCCCHHHHHHHHEECCCCCCCCCCC
PYVASLFVTCPEDDRQTSLTVNPHASLPMPRMSCPPADVRIQSINDYGGISAPFTPPLDG
CEEEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
PVSTPH
CCCCCC
>Mature Secondary Structure 
TAPHATPRRASAAARRLPYRVAAVALAVAALLPWQSAHADVRLSKTRVIYAASHPDATL
CCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEECEEEEEEECCCCEEE
TVSNAGTEPAIVQTWIETADDKLDTPFFAAPPLFRLDPGDTNLIRVTLVGQAPDTRVERL
EEECCCCCCHHHHHHHHHCCCCCCCCEECCCCEEEECCCCCCEEEEEEECCCCCCCEEEE
YWLNVKEIPPAPQASAGNTLQFAIRTRIKLLYRPPGLDGAFDLQRDLRLSRGGASVENTS
EEECCCCCCCCCCCCCCCEEEEEEEEEEEEEECCCCCCCHHHHHHHHEECCCCCCCCCCC
PYVASLFVTCPEDDRQTSLTVNPHASLPMPRMSCPPADVRIQSINDYGGISAPFTPPLDG
CEEEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
PVSTPH
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7721701; 11677609 [H]