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The map label for this gene is gacS [H]

Identifier: 238027848

GI number: 238027848

Start: 2486388

End: 2487890

Strand: Direct

Name: gacS [H]

Synonym: bglu_1g22790

Alternate gene names: 238027848

Gene position: 2486388-2487890 (Clockwise)

Preceding gene: 238027847

Following gene: 238027849

Centisome position: 63.65

GC content: 70.06

Gene sequence:

>1503_bases
ATGGCCATCCAGATACAGCTCATTTCGCTCGATTCCGAGGAACAGGTGGCGGTCGCGCGCCGCCAGGCACGCGTGATCGC
CGCCGGCCTCGGTTTCGCGCCACTCGATCAGACCCGCATCGCCAGCGCGGTGTCGGAGATCGCCCGCAACGCGCTCGATC
ACGCCGGCGGCGGCACCGTGTGGTTCGGCTTCGACCTGCAGGCCCGGCCGCAGGCGCTCATGGTGCAGGTGCGCGACGAC
GGCCCCGGAATCCACAATCTGCAGGCCGTGCTCGACGGCAGCCATCGCTCCGAGTCCGGCATGGCGGGCAACGGCATCGC
CAGTTGTCGGCGTCTGATGGACCGCTGCACGATCGAATCGGCGGCCGGCCGCGGCACCACCGTGACCATGCTGCGCGCGC
TGCCGCAGGACCGGCCGCCGATGGCGGCATCGGCCGTGCAGGCGCTCGTCGAGGCGCTGCGCGGCGACCTGCCCGCCCCG
CCGGCCGCCCCCGGCACGTCCGCCGCGCCCGCCGCGCCGCGCTCGCTTGCCCACCCGCCGCGCAACCCGTTCGCCGAGCT
GCGGCATCAGAACCGCGAACTGCTGGCCGCGCTGACCGAGCTGCAGGCACGCCAGGAGGACCTGTCGCGCCTGACGAGCG
AGCTGGAGGCCACCAACCGCGGCGTGGTCGCGCTCTACGCCGAGCTCGACGAGCGCGCCGAGGAGCTGCGCCGCGTCGAT
TCGCTCAAGACCCGCTTCCTGTCCGACATGAGCCACGAGCTGCGCACCCCGCTCAGCTCGATCCGCGCGCTGTCGAATCT
GCTGCTGAACCGGCTCGACGGCGAACTGACCGGCGAACAGGAACGGCAAGTGCTGCTGATCCGCAAATCGGCCGAGGATC
TGACCGACATCGTCAACGACCTGCTCGACCTGGCGAAGATCGAGGCAGGCCGCACCGACGTGCTGCCGGTGCTGTTCGAG
CCGCACACCCTGGTGGCCGCGGTGCGTGCCACGCTCGCGCCGCTGCTGAGCAGCCCGGCCGTGCAGCTGATCTTCGACGA
CATGCGGGGGCTGCCGGCGATGCTCACGGACGAGGCGAAGGTCACTCAGATTCTGCGCAACTTTGTATCGAACGCGCTCA
AATTTACAGAGCGCGGCGAAATTCGCGTCAGTGCGCGCTTGCAGCCCGACGACGAGCACATCACGTTCTTCGTCACGGAC
ACGGGCATCGGCATCGCGCAGGAGGATCAGGAATTGATATTCGAGGCATTTGGTCAGGTCCGCAGCCATCTGCAGCAACG
CGTCAAGGGCACCGGGCTCGGCCTGCCGCTTTGCCGCAACCTCGCCGCGCTGCTGGGCGGCCACGTCGGCGTGGAGAGCC
GGCTCGGCGAAGGCTCGACGTTCTACGCGACGCTGCCGCTGCGCTTCGCGGCCCCGGCCCAGGCGCACCACGACGGCGCC
GCCGCTGCCGGCGGCCTCACCGACACCCCGACTTCGCGTGGAGATGACGCATGGCCGCGTTAA

Upstream 100 bases:

>100_bases
CGACGCGACGGTGGTCGTGGTCAAGACGGCGCCGTCACGCTGACGAACCGGGCCGACGAACCAGGGCGATACCGAGACTC
ACGAACCGATCCATCCGAGC

Downstream 100 bases:

>100_bases
GCCCGCTGATCCTCAACGTCGACGACAACGAGGGCGCCCGCTATGCCAAGACGCGCGTGCTGATCCACGCCGGCTACCAG
GTGATCGAGGCCGGCACCGG

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MAIQIQLISLDSEEQVAVARRQARVIAAGLGFAPLDQTRIASAVSEIARNALDHAGGGTVWFGFDLQARPQALMVQVRDD
GPGIHNLQAVLDGSHRSESGMAGNGIASCRRLMDRCTIESAAGRGTTVTMLRALPQDRPPMAASAVQALVEALRGDLPAP
PAAPGTSAAPAAPRSLAHPPRNPFAELRHQNRELLAALTELQARQEDLSRLTSELEATNRGVVALYAELDERAEELRRVD
SLKTRFLSDMSHELRTPLSSIRALSNLLLNRLDGELTGEQERQVLLIRKSAEDLTDIVNDLLDLAKIEAGRTDVLPVLFE
PHTLVAAVRATLAPLLSSPAVQLIFDDMRGLPAMLTDEAKVTQILRNFVSNALKFTERGEIRVSARLQPDDEHITFFVTD
TGIGIAQEDQELIFEAFGQVRSHLQQRVKGTGLGLPLCRNLAALLGGHVGVESRLGEGSTFYATLPLRFAAPAQAHHDGA
AAAGGLTDTPTSRGDDAWPR

Sequences:

>Translated_500_residues
MAIQIQLISLDSEEQVAVARRQARVIAAGLGFAPLDQTRIASAVSEIARNALDHAGGGTVWFGFDLQARPQALMVQVRDD
GPGIHNLQAVLDGSHRSESGMAGNGIASCRRLMDRCTIESAAGRGTTVTMLRALPQDRPPMAASAVQALVEALRGDLPAP
PAAPGTSAAPAAPRSLAHPPRNPFAELRHQNRELLAALTELQARQEDLSRLTSELEATNRGVVALYAELDERAEELRRVD
SLKTRFLSDMSHELRTPLSSIRALSNLLLNRLDGELTGEQERQVLLIRKSAEDLTDIVNDLLDLAKIEAGRTDVLPVLFE
PHTLVAAVRATLAPLLSSPAVQLIFDDMRGLPAMLTDEAKVTQILRNFVSNALKFTERGEIRVSARLQPDDEHITFFVTD
TGIGIAQEDQELIFEAFGQVRSHLQQRVKGTGLGLPLCRNLAALLGGHVGVESRLGEGSTFYATLPLRFAAPAQAHHDGA
AAAGGLTDTPTSRGDDAWPR
>Mature_499_residues
AIQIQLISLDSEEQVAVARRQARVIAAGLGFAPLDQTRIASAVSEIARNALDHAGGGTVWFGFDLQARPQALMVQVRDDG
PGIHNLQAVLDGSHRSESGMAGNGIASCRRLMDRCTIESAAGRGTTVTMLRALPQDRPPMAASAVQALVEALRGDLPAPP
AAPGTSAAPAAPRSLAHPPRNPFAELRHQNRELLAALTELQARQEDLSRLTSELEATNRGVVALYAELDERAEELRRVDS
LKTRFLSDMSHELRTPLSSIRALSNLLLNRLDGELTGEQERQVLLIRKSAEDLTDIVNDLLDLAKIEAGRTDVLPVLFEP
HTLVAAVRATLAPLLSSPAVQLIFDDMRGLPAMLTDEAKVTQILRNFVSNALKFTERGEIRVSARLQPDDEHITFFVTDT
GIGIAQEDQELIFEAFGQVRSHLQQRVKGTGLGLPLCRNLAALLGGHVGVESRLGEGSTFYATLPLRFAAPAQAHHDGAA
AAGGLTDTPTSRGDDAWPR

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI48994928, Length=238, Percent_Identity=40.7563025210084, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI1789149, Length=270, Percent_Identity=34.0740740740741, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI145693157, Length=289, Percent_Identity=30.7958477508651, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1788713, Length=234, Percent_Identity=32.4786324786325, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI87081816, Length=353, Percent_Identity=33.1444759206799, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1786912, Length=254, Percent_Identity=29.1338582677165, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI87082128, Length=237, Percent_Identity=29.957805907173, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1790436, Length=239, Percent_Identity=29.2887029288703, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790346, Length=254, Percent_Identity=28.740157480315, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1786600, Length=238, Percent_Identity=26.4705882352941, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788549, Length=277, Percent_Identity=27.0758122743682, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788393, Length=261, Percent_Identity=25.2873563218391, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786783, Length=254, Percent_Identity=26.7716535433071, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53874; Mature: 53743

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIQIQLISLDSEEQVAVARRQARVIAAGLGFAPLDQTRIASAVSEIARNALDHAGGGTV
CEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEE
WFGFDLQARPQALMVQVRDDGPGIHNLQAVLDGSHRSESGMAGNGIASCRRLMDRCTIES
EEECCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AAGRGTTVTMLRALPQDRPPMAASAVQALVEALRGDLPAPPAAPGTSAAPAAPRSLAHPP
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCC
RNPFAELRHQNRELLAALTELQARQEDLSRLTSELEATNRGVVALYAELDERAEELRRVD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHH
SLKTRFLSDMSHELRTPLSSIRALSNLLLNRLDGELTGEQERQVLLIRKSAEDLTDIVND
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHH
LLDLAKIEAGRTDVLPVLFEPHTLVAAVRATLAPLLSSPAVQLIFDDMRGLPAMLTDEAK
HHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHCCHHH
VTQILRNFVSNALKFTERGEIRVSARLQPDDEHITFFVTDTGIGIAQEDQELIFEAFGQV
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHH
RSHLQQRVKGTGLGLPLCRNLAALLGGHVGVESRLGEGSTFYATLPLRFAAPAQAHHDGA
HHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHCCCCCCEEEEEECEEECCCCHHCCCCC
AAAGGLTDTPTSRGDDAWPR
HHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AIQIQLISLDSEEQVAVARRQARVIAAGLGFAPLDQTRIASAVSEIARNALDHAGGGTV
EEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEE
WFGFDLQARPQALMVQVRDDGPGIHNLQAVLDGSHRSESGMAGNGIASCRRLMDRCTIES
EEECCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AAGRGTTVTMLRALPQDRPPMAASAVQALVEALRGDLPAPPAAPGTSAAPAAPRSLAHPP
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCC
RNPFAELRHQNRELLAALTELQARQEDLSRLTSELEATNRGVVALYAELDERAEELRRVD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHH
SLKTRFLSDMSHELRTPLSSIRALSNLLLNRLDGELTGEQERQVLLIRKSAEDLTDIVND
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHH
LLDLAKIEAGRTDVLPVLFEPHTLVAAVRATLAPLLSSPAVQLIFDDMRGLPAMLTDEAK
HHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHCCHHH
VTQILRNFVSNALKFTERGEIRVSARLQPDDEHITFFVTDTGIGIAQEDQELIFEAFGQV
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHH
RSHLQQRVKGTGLGLPLCRNLAALLGGHVGVESRLGEGSTFYATLPLRFAAPAQAHHDGA
HHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHCCCCCCEEEEEECEEECCCCHHCCCCC
AAAGGLTDTPTSRGDDAWPR
HHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]