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The map label for this gene is lgrC [H]

Identifier: 238027312

GI number: 238027312

Start: 1885751

End: 1887238

Strand: Reverse

Name: lgrC [H]

Synonym: bglu_1g17000

Alternate gene names: 238027312

Gene position: 1887238-1885751 (Counterclockwise)

Preceding gene: 238027315

Following gene: 238027311

Centisome position: 48.31

GC content: 70.23

Gene sequence:

>1488_bases
TTGACCTCCGTCAGCGTCAACCGGCTGTTCGATTCCCAAGCGCGGCTGAACCCCGGCGCGATCGCCGTGTCGGGTGGCGG
CACGCATCTCACCTACGCGCAGCTGGCGCGCTGCGCCGATCATCTCGCGCGGCGTCTCGTCGAGGCGGGGGTCAGGCCGC
TCGATCGCGTGCTGCTGTGCCTGCCGCGCTCGCTCGACGCGGTAATCGCGATGCTCGCCGTGGCCAAGACGGGCGCGGCG
TTCGTCCCGGTGGATCCGTCCTATGCCGCACCGGTCCTGCACGCCTACGCGCTCGATAGCGGCGCGCGCTACGCGCTGGT
GCGTCCTGGCGAAGGCGCGGCGCTGGGCGAGGCGGCACGGCTCATCGAGGCGCACGATCTCGCTGCCGCGCGCGACGCCG
ACGCCCCCGTGGTGGATGCAGGCCACGACGGCGAAGCGCCGGTCTACGTGATGTTCACTTCGGGCAGCACGGGCCGCCCG
AAGGGCGTGATCGTGCCGCATCGGGGCGTGGTACGTCTGGTGCGCGACACCAACTACATCCGGATCGACGCGACCGATAC
GCTCGCGCTGCTCTCGCCCATCACCTTCGACGCCTCGACCTTCGAGATCTGGGGCGCGCTGCTCAACGGCGCGCGGCTCG
CGGTCTACCAGGAGCCGGGCTTCGATCCGAACGCGGTCGGCCGGCTGGTGGCCGAGCAGCGCGTGACGGTGATGTGGCTG
ACCGCTGCGCTGTTCCATCTGGTGGCGCGCCGCTTCGTGCGGCTGCTCGACGGCGTGCGGGTACTGCTGGCGGGCGGCGA
CGTGCTGCACGCGAAAGCGGTCCACGCGGTATTCGACGCCCACCCCGGGATCATCCTCGTCAACGGCTACGGGCCGACCG
AGAACACTACCTTCACCTGCTGCCACGTGATGCGCAATGCCGAGCGCCCGCAAGGCTCCGTGCCGATCGGCCGCGCGATC
ACGGGCACCACGCTGTGGGTGCTCGACGAAGCGCTGCAGCCGGTGCCCGACGGCACGGAAGGCGAGCTCTGCGTGGGCGG
CGCCGGTGTCGCGCTCGGTTATCTGAACGCCCCCGAGGCGACGCGTGCCGCGTTCCTCACCTGGCCGCACCAGCACGGCC
TGCTGTATCGCACCGGCGACCGGGTACGGCGCGGGCATGACGGCGTGGTCGAATTCCTCGGTCGCAAGGACCGGCTGGTG
AAGATTCGCGGCTATCGCGTCTCGCTCGACGAACTGCAGAAAGTGATCGCGACGATTCCAGGCGTGGAAGAGGCGATCGT
CTCGGTATCGGAGGACACGCTCGGCGAGCGGCGGCTCACCGCGACCCTGCAGGCCGCCGATGCCGGCCCCGAGCAGCAGG
CCTTCGTGCGCCGCGAGCTGCGCAAGCGCGTGCCGCCGTTCCAGATTCCCGACGAGATCCATATCCATTCCCACCTGCCG
TTGAACGCCAACGGCAAGCTCGACCGGCACCGCGTGCCGGCTGCATGA

Upstream 100 bases:

>100_bases
TGACCGCCGTCGCCGCGCCGCCCTGCTCCGGTCGCCGTTCGGTGCTGGCTTCATACCGGGCATCATTCGGCATCCTATTT
TTTCGGGGAGATTCATCATC

Downstream 100 bases:

>100_bases
CGAGCCCCTGCCTATCCGGAGAGAAGCTATGACCAACACCATCGATATCACCGAAACCATCCAGGCGACTTGCCGCGAGC
TGTTGAAGTTGCCCGACCTC

Product: peptide synthetase

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: ATP-dependent valine adenylase; ValA; Valine activase; ATP-dependent D-valine adenylase; D-ValA; D-valine activase; Valine racemase [ATP-hydrolyzing]; ATP-dependent tryptophan adenylase; TrpA; Tryptophan activase; ATP-dependent D-leucine adenylase; D-LeuA; D-leucine activase; Leucine racemase [ATP-hydrolyzing]; ATP-dependent tryptophan/phenylalanine/tyrosine adenylase; Trp/Phe/TyrA; Tryptophan/phenylalanine/tyrosine activase; ATP-dependent D-leucine adenylase; D-LeuA; D-leucine activase; Leucine racemase [ATP-hydrolyzing] [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MTSVSVNRLFDSQARLNPGAIAVSGGGTHLTYAQLARCADHLARRLVEAGVRPLDRVLLCLPRSLDAVIAMLAVAKTGAA
FVPVDPSYAAPVLHAYALDSGARYALVRPGEGAALGEAARLIEAHDLAAARDADAPVVDAGHDGEAPVYVMFTSGSTGRP
KGVIVPHRGVVRLVRDTNYIRIDATDTLALLSPITFDASTFEIWGALLNGARLAVYQEPGFDPNAVGRLVAEQRVTVMWL
TAALFHLVARRFVRLLDGVRVLLAGGDVLHAKAVHAVFDAHPGIILVNGYGPTENTTFTCCHVMRNAERPQGSVPIGRAI
TGTTLWVLDEALQPVPDGTEGELCVGGAGVALGYLNAPEATRAAFLTWPHQHGLLYRTGDRVRRGHDGVVEFLGRKDRLV
KIRGYRVSLDELQKVIATIPGVEEAIVSVSEDTLGERRLTATLQAADAGPEQQAFVRRELRKRVPPFQIPDEIHIHSHLP
LNANGKLDRHRVPAA

Sequences:

>Translated_495_residues
MTSVSVNRLFDSQARLNPGAIAVSGGGTHLTYAQLARCADHLARRLVEAGVRPLDRVLLCLPRSLDAVIAMLAVAKTGAA
FVPVDPSYAAPVLHAYALDSGARYALVRPGEGAALGEAARLIEAHDLAAARDADAPVVDAGHDGEAPVYVMFTSGSTGRP
KGVIVPHRGVVRLVRDTNYIRIDATDTLALLSPITFDASTFEIWGALLNGARLAVYQEPGFDPNAVGRLVAEQRVTVMWL
TAALFHLVARRFVRLLDGVRVLLAGGDVLHAKAVHAVFDAHPGIILVNGYGPTENTTFTCCHVMRNAERPQGSVPIGRAI
TGTTLWVLDEALQPVPDGTEGELCVGGAGVALGYLNAPEATRAAFLTWPHQHGLLYRTGDRVRRGHDGVVEFLGRKDRLV
KIRGYRVSLDELQKVIATIPGVEEAIVSVSEDTLGERRLTATLQAADAGPEQQAFVRRELRKRVPPFQIPDEIHIHSHLP
LNANGKLDRHRVPAA
>Mature_494_residues
TSVSVNRLFDSQARLNPGAIAVSGGGTHLTYAQLARCADHLARRLVEAGVRPLDRVLLCLPRSLDAVIAMLAVAKTGAAF
VPVDPSYAAPVLHAYALDSGARYALVRPGEGAALGEAARLIEAHDLAAARDADAPVVDAGHDGEAPVYVMFTSGSTGRPK
GVIVPHRGVVRLVRDTNYIRIDATDTLALLSPITFDASTFEIWGALLNGARLAVYQEPGFDPNAVGRLVAEQRVTVMWLT
AALFHLVARRFVRLLDGVRVLLAGGDVLHAKAVHAVFDAHPGIILVNGYGPTENTTFTCCHVMRNAERPQGSVPIGRAIT
GTTLWVLDEALQPVPDGTEGELCVGGAGVALGYLNAPEATRAAFLTWPHQHGLLYRTGDRVRRGHDGVVEFLGRKDRLVK
IRGYRVSLDELQKVIATIPGVEEAIVSVSEDTLGERRLTATLQAADAGPEQQAFVRRELRKRVPPFQIPDEIHIHSHLPL
NANGKLDRHRVPAA

Specific function: Activates the 7th to 12th amino acids (Val, D-Val, Trp, D-Leu, Xaa and D-Leu) in linear gramicidin and catalyzes the formation of the peptide bond between them. This enzyme is also responsible for the epimerization of the 8th (D-Val), the 10th (D- Leu) an

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 6 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=550, Percent_Identity=24.7272727272727, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI38505220, Length=511, Percent_Identity=24.6575342465753, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI45580730, Length=523, Percent_Identity=24.8565965583174, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI28416953, Length=547, Percent_Identity=25.2285191956124, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI157311624, Length=521, Percent_Identity=23.0326295585413, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI157311622, Length=521, Percent_Identity=23.0326295585413, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI58082049, Length=525, Percent_Identity=22.6666666666667, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI122937307, Length=515, Percent_Identity=22.3300970873786, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI115511026, Length=520, Percent_Identity=21.7307692307692, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI42544132, Length=514, Percent_Identity=20.8171206225681, Blast_Score=83, Evalue=5e-16,
Organism=Escherichia coli, GI1786801, Length=524, Percent_Identity=31.2977099236641, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI145693145, Length=519, Percent_Identity=26.7822736030828, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1790505, Length=520, Percent_Identity=23.0769230769231, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788107, Length=551, Percent_Identity=23.7749546279492, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789201, Length=360, Percent_Identity=27.5, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786810, Length=516, Percent_Identity=24.4186046511628, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI221142682, Length=225, Percent_Identity=25.7777777777778, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17556356, Length=502, Percent_Identity=24.3027888446215, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=498, Percent_Identity=24.0963855421687, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17559526, Length=365, Percent_Identity=24.9315068493151, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17550940, Length=344, Percent_Identity=24.7093023255814, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319591, Length=559, Percent_Identity=24.6869409660107, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6319699, Length=529, Percent_Identity=22.3062381852552, Blast_Score=83, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24648676, Length=535, Percent_Identity=28.411214953271, Blast_Score=152, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24582852, Length=451, Percent_Identity=25.7206208425721, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19922652, Length=501, Percent_Identity=23.3532934131737, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI18859661, Length=438, Percent_Identity=25.3424657534247, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24648260, Length=290, Percent_Identity=24.8275862068966, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR023213
- InterPro:   IPR001242
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 53156; Mature: 53024

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVSVNRLFDSQARLNPGAIAVSGGGTHLTYAQLARCADHLARRLVEAGVRPLDRVLLC
CCCCCHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LPRSLDAVIAMLAVAKTGAAFVPVDPSYAAPVLHAYALDSGARYALVRPGEGAALGEAAR
CCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHEECCCCEEEEEECCCCCCHHHHHH
LIEAHDLAAARDADAPVVDAGHDGEAPVYVMFTSGSTGRPKGVIVPHRGVVRLVRDTNYI
HHHHHHHHHCCCCCCCEEECCCCCCCCEEEEEECCCCCCCCEEEECCCCCEEEEECCCEE
RIDATDTLALLSPITFDASTFEIWGALLNGARLAVYQEPGFDPNAVGRLVAEQRVTVMWL
EEECCCHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHH
TAALFHLVARRFVRLLDGVRVLLAGGDVLHAKAVHAVFDAHPGIILVNGYGPTENTTFTC
HHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHH
CHVMRNAERPQGSVPIGRAITGTTLWVLDEALQPVPDGTEGELCVGGAGVALGYLNAPEA
HHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCEEEEECCCCCH
TRAAFLTWPHQHGLLYRTGDRVRRGHDGVVEFLGRKDRLVKIRGYRVSLDELQKVIATIP
HCEEEEECCCCCCCEEECCHHHHCCCHHHHHHHCCCCCEEEEECEEECHHHHHHHHHHCC
GVEEAIVSVSEDTLGERRLTATLQAADAGPEQQAFVRRELRKRVPPFQIPDEIHIHSHLP
CHHHHHHCCCHHHCCCHHEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCC
LNANGKLDRHRVPAA
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSVSVNRLFDSQARLNPGAIAVSGGGTHLTYAQLARCADHLARRLVEAGVRPLDRVLLC
CCCCHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LPRSLDAVIAMLAVAKTGAAFVPVDPSYAAPVLHAYALDSGARYALVRPGEGAALGEAAR
CCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHEECCCCEEEEEECCCCCCHHHHHH
LIEAHDLAAARDADAPVVDAGHDGEAPVYVMFTSGSTGRPKGVIVPHRGVVRLVRDTNYI
HHHHHHHHHCCCCCCCEEECCCCCCCCEEEEEECCCCCCCCEEEECCCCCEEEEECCCEE
RIDATDTLALLSPITFDASTFEIWGALLNGARLAVYQEPGFDPNAVGRLVAEQRVTVMWL
EEECCCHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHH
TAALFHLVARRFVRLLDGVRVLLAGGDVLHAKAVHAVFDAHPGIILVNGYGPTENTTFTC
HHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHH
CHVMRNAERPQGSVPIGRAITGTTLWVLDEALQPVPDGTEGELCVGGAGVALGYLNAPEA
HHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCEEEEECCCCCH
TRAAFLTWPHQHGLLYRTGDRVRRGHDGVVEFLGRKDRLVKIRGYRVSLDELQKVIATIP
HCEEEEECCCCCCCEEECCHHHHCCCHHHHHHHCCCCCEEEEECEEECHHHHHHHHHHCC
GVEEAIVSVSEDTLGERRLTATLQAADAGPEQQAFVRRELRKRVPPFQIPDEIHIHSHLP
CHHHHHHCCCHHHCCCHHEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCC
LNANGKLDRHRVPAA
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA