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The map label for this gene is gcvA [H]

Identifier: 238027298

GI number: 238027298

Start: 1854850

End: 1855773

Strand: Reverse

Name: gcvA [H]

Synonym: bglu_1g16850

Alternate gene names: 238027298

Gene position: 1855773-1854850 (Counterclockwise)

Preceding gene: 238027300

Following gene: 238027297

Centisome position: 47.5

GC content: 72.62

Gene sequence:

>924_bases
ATGACAGCCCCGCTCATCAAACTTCCTTCGCTCGATTTGATGCGCGGCTACGTGGCCGTCGGCCGCCGCATGAGCATCAC
GCTCGCCGCCCAGGACCTGTGCCTCACCCAATCGGCCGTGAGCCGGCAGGTCAACGCGCTGGAGGAAGCGCTCGGCGTCC
GCCTGTTTCATCGCGGCTATCGTTCGATTTCGTTTACGCCCGAGGGCGAGCGGCTGTTTCGCGCCGCCGACGGCGCGGTG
CGCCAGTTGCAGGACACCATCGAGGCGCTGACCCATCCGAAGGTGCGCCAGCCCGTGACGATCACGGCCAGCATCGGCGT
GGCGAGCCTCTGGCTGCTGCCGCGGCTCGGCGAGCTGCAACGCATCCACCCCGGGGTCGATCTGCGCGTGGCGGCCACCG
ACAAGCTGCTCGACCTGCGCGCCGAGGGCATCGACCTGGCGATCCGCTACGCGCCCGCCGACCATGCGCCGGCCACCTCG
CTGCGCCTGTTCGACGAGACCATCGTGCCGGTCGCCTCGCCCTCGCTCGGCGTGGCGACGCTCGACGCCGGGACGCTGGC
GCGCCACGTGCTGCTCGAATTCGACGGCGCGCGGCGCCCGCTGCTGCAATGGGCCGACCATCTCGGCGCGCTCGGGCTCG
ATGCCGCGCAGGCGCGCGGCATGCTGCGCTTCAACCAGTACGACCAGGTGATCCAGGCCGCGCTGGCCGGGCGCGGCATC
GCGCTCGGCCGCATCGCGCTGGTCGAGCCGCTGCTCGCCGACGGCAGGCTGGTGGCGATCGGCGCCCCCGGCGCGCCGCG
CGCGAGCGGCCACGCCTACTGGCTCCACCAGGCCGAGCTGACGCCGCGCGAGGACGTGCGTGCCGTGATCGACTGGATTC
TCGCGGCCACGCGCGCCGGCGGCGAGCCTGACTCGGCGGCCTGA

Upstream 100 bases:

>100_bases
CTATTCCGATCAATCGGGGCAGGTTTCCTGACCGTTATTGCACTTGACCTCCCCGTTTAACTCAATCAATCTTCTCGCAT
CGCTCGGATGAGGCACGCGC

Downstream 100 bases:

>100_bases
CGCGGCCCCGCCCCCGCCCGCCTCTACCAAAGCTGGTAGTTGTCCCCGCGGCGGCCAGCTAGTGAAATCGTGAGCATCTC
AACGACGGTCCCGCACCGTG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTAPLIKLPSLDLMRGYVAVGRRMSITLAAQDLCLTQSAVSRQVNALEEALGVRLFHRGYRSISFTPEGERLFRAADGAV
RQLQDTIEALTHPKVRQPVTITASIGVASLWLLPRLGELQRIHPGVDLRVAATDKLLDLRAEGIDLAIRYAPADHAPATS
LRLFDETIVPVASPSLGVATLDAGTLARHVLLEFDGARRPLLQWADHLGALGLDAAQARGMLRFNQYDQVIQAALAGRGI
ALGRIALVEPLLADGRLVAIGAPGAPRASGHAYWLHQAELTPREDVRAVIDWILAATRAGGEPDSAA

Sequences:

>Translated_307_residues
MTAPLIKLPSLDLMRGYVAVGRRMSITLAAQDLCLTQSAVSRQVNALEEALGVRLFHRGYRSISFTPEGERLFRAADGAV
RQLQDTIEALTHPKVRQPVTITASIGVASLWLLPRLGELQRIHPGVDLRVAATDKLLDLRAEGIDLAIRYAPADHAPATS
LRLFDETIVPVASPSLGVATLDAGTLARHVLLEFDGARRPLLQWADHLGALGLDAAQARGMLRFNQYDQVIQAALAGRGI
ALGRIALVEPLLADGRLVAIGAPGAPRASGHAYWLHQAELTPREDVRAVIDWILAATRAGGEPDSAA
>Mature_306_residues
TAPLIKLPSLDLMRGYVAVGRRMSITLAAQDLCLTQSAVSRQVNALEEALGVRLFHRGYRSISFTPEGERLFRAADGAVR
QLQDTIEALTHPKVRQPVTITASIGVASLWLLPRLGELQRIHPGVDLRVAATDKLLDLRAEGIDLAIRYAPADHAPATSL
RLFDETIVPVASPSLGVATLDAGTLARHVLLEFDGARRPLLQWADHLGALGLDAAQARGMLRFNQYDQVIQAALAGRGIA
LGRIALVEPLLADGRLVAIGAPGAPRASGHAYWLHQAELTPREDVRAVIDWILAATRAGGEPDSAA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=303, Percent_Identity=32.6732673267327, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1788706, Length=272, Percent_Identity=32.3529411764706, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI1786448, Length=249, Percent_Identity=32.9317269076305, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI145693193, Length=267, Percent_Identity=29.5880149812734, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081978, Length=265, Percent_Identity=30.188679245283, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=26.7361111111111, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787128, Length=135, Percent_Identity=28.8888888888889, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1787589, Length=283, Percent_Identity=24.7349823321555, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32954; Mature: 32823

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPLIKLPSLDLMRGYVAVGRRMSITLAAQDLCLTQSAVSRQVNALEEALGVRLFHRGY
CCCCCEECCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RSISFTPEGERLFRAADGAVRQLQDTIEALTHPKVRQPVTITASIGVASLWLLPRLGELQ
CEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHCCCCCHH
RIHPGVDLRVAATDKLLDLRAEGIDLAIRYAPADHAPATSLRLFDETIVPVASPSLGVAT
HCCCCCCEEEECCHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCEEE
LDAGTLARHVLLEFDGARRPLLQWADHLGALGLDAAQARGMLRFNQYDQVIQAALAGRGI
ECHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCC
ALGRIALVEPLLADGRLVAIGAPGAPRASGHAYWLHQAELTPREDVRAVIDWILAATRAG
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCC
GEPDSAA
CCCCCCC
>Mature Secondary Structure 
TAPLIKLPSLDLMRGYVAVGRRMSITLAAQDLCLTQSAVSRQVNALEEALGVRLFHRGY
CCCCEECCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RSISFTPEGERLFRAADGAVRQLQDTIEALTHPKVRQPVTITASIGVASLWLLPRLGELQ
CEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHCCCCCHH
RIHPGVDLRVAATDKLLDLRAEGIDLAIRYAPADHAPATSLRLFDETIVPVASPSLGVAT
HCCCCCCEEEECCHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCEEE
LDAGTLARHVLLEFDGARRPLLQWADHLGALGLDAAQARGMLRFNQYDQVIQAALAGRGI
ECHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCC
ALGRIALVEPLLADGRLVAIGAPGAPRASGHAYWLHQAELTPREDVRAVIDWILAATRAG
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCC
GEPDSAA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]