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The map label for this gene is actII-2 [H]

Identifier: 238027281

GI number: 238027281

Start: 1835296

End: 1836777

Strand: Direct

Name: actII-2 [H]

Synonym: bglu_1g16680

Alternate gene names: 238027281

Gene position: 1835296-1836777 (Clockwise)

Preceding gene: 238027280

Following gene: 238027282

Centisome position: 46.98

GC content: 74.16

Gene sequence:

>1482_bases
ATGTCGAATTCCTCCACCCTCGCGCCGGGCGCCGCCGCCCCGGTCCGGCCGCATCGCCGCATGCTGCCGCTGCTGCTGAT
CGGGCAGGGCATGGCGGCGATGGATACCTCGATCGTCAACGTCGCGGCGCCCGCGCTGCGCGCCGACCTGGGCATCTCGG
GCGCGCTGCTGCAGCTCGTGGTGGCCGGCTACATCCTCGCCTACGCCGTGTTCCTGATCACCGGCGCGCGCCTCGGCGAC
GATCACGGCTACCGGCGCCTGTTCATCCACGGCCTGGCGATCTTCACGATCTCGTCGCTGGCCTGCGGCCTGGCGCCCGA
CACCTGGCTGCTGATCGCTGGGCGCCTCGCGCAGGGCGTGGGCGCGGCGATGCTGGTGCCGCAGGTGATGTCGCTGATCC
AGCGCAGCTACGAGGGCCGGGAGCGGGCCCGCGCGATCGGCTACTACTCGATGATCCTCGGGCTCGGCTCCACGGCCGGC
CAGGCGCTCGGCGGGCTGATCATCACCGCGAACCTGGGCGGCCTGTCGTGGCGCCCGGCGTTCCTCATCAACGTGCCGAT
CGGCATCGTGCTCTTGCTGTACGCGCCCGCGGTGCTGCCGGACCTGGCCGGCGCCAAGCGGCGCCGGCTCGATCTGGCCG
GCGTGGCGCTGCTGACGGCGGCGATGCTGCTGGTGGTGGTGCCGCTCACGTTCGGCCACGACGCCGGCTGGGCCGCCTGG
GTGTGGGTCGCGCTGGCCGCCGGGGTGGCCGGGCTGGCGGTGTTCCTGCGCTTCGAGCACGGCCTCGCGCGGCGCGGCGC
GGCCCCGCTGCTGGATCTGGACGCGCTGCTCGCGCCCGGCATCCGTCCCGGCCTGGCGGTGGTGTCGATCGGCTTCATCG
GCTACGGCGGCTGGCTGTTCGCCTGCGCGCTGTACCTGCAGACCGGGCTGGGCGATTCGCCGATGATCTCCGGCTTCGTG
TTCGCGGCCTACGCATTCGGCTTCGGCATCTCGAACGTGAGCTGGTCGAAGCTGCCGGCGCGGCTGCTGCGCTTCACGCC
CGCCGCGGCGCTGGCCGTGATGGTGGTGGCCAACGCCGCGTTCGGCACCGGCGCGCGCCAGTTCGGCTGGCTGGCCGCGC
TGATGGTGCCGCTGCTGTTCCTGGCCGGCGCGAGCCACGGCCTCACGTTCGGCACCACCGTCAGCCAGATGACGCAGCGC
ACCGCGGCGCGCCATGCGCCCGCGTTGAGCGGCCTCGTCTCCACCTCGGTGCAGCTGTCGATCGTGATCGGCGTGGCCGC
GCTCGGCTCGGTCTATCTGGCCATCGCACGGCCCGGCGACGTGCCGCGCTCGGCGCAGGCCATCTCGAGCGTCACCTTCG
CGATCGCGGCGCTGGCGCTGGTGGCGATCGCCTGCTCGCTGCGGCTCGCGACGATGCGGGCGGCGCCGCCGGCCGCCGTG
CCCGCCGCGCGGCCCGGCGGCGACGCGCCGCGGCCGCACTGA

Upstream 100 bases:

>100_bases
CAAGCGGGCGGCACTGCCCGACGCCGCGCGCTGGAAGGACGTCGAGGGCAAGCTCGAACACCTGAAGCGCGACGTGATCG
CGTAAGGCGAGGGAGCCGGC

Downstream 100 bases:

>100_bases
GCGCGAGATGCGCCGCGCGCTCCGCTACCGGATGCAACCCGATACCGCGAGGGTCGCCACGACCGTCGCGGCCACTCCGA
CCACCACGAGGATTCCGACT

Product: major facilitator superfamily protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSNSSTLAPGAAAPVRPHRRMLPLLLIGQGMAAMDTSIVNVAAPALRADLGISGALLQLVVAGYILAYAVFLITGARLGD
DHGYRRLFIHGLAIFTISSLACGLAPDTWLLIAGRLAQGVGAAMLVPQVMSLIQRSYEGRERARAIGYYSMILGLGSTAG
QALGGLIITANLGGLSWRPAFLINVPIGIVLLLYAPAVLPDLAGAKRRRLDLAGVALLTAAMLLVVVPLTFGHDAGWAAW
VWVALAAGVAGLAVFLRFEHGLARRGAAPLLDLDALLAPGIRPGLAVVSIGFIGYGGWLFACALYLQTGLGDSPMISGFV
FAAYAFGFGISNVSWSKLPARLLRFTPAAALAVMVVANAAFGTGARQFGWLAALMVPLLFLAGASHGLTFGTTVSQMTQR
TAARHAPALSGLVSTSVQLSIVIGVAALGSVYLAIARPGDVPRSAQAISSVTFAIAALALVAIACSLRLATMRAAPPAAV
PAARPGGDAPRPH

Sequences:

>Translated_493_residues
MSNSSTLAPGAAAPVRPHRRMLPLLLIGQGMAAMDTSIVNVAAPALRADLGISGALLQLVVAGYILAYAVFLITGARLGD
DHGYRRLFIHGLAIFTISSLACGLAPDTWLLIAGRLAQGVGAAMLVPQVMSLIQRSYEGRERARAIGYYSMILGLGSTAG
QALGGLIITANLGGLSWRPAFLINVPIGIVLLLYAPAVLPDLAGAKRRRLDLAGVALLTAAMLLVVVPLTFGHDAGWAAW
VWVALAAGVAGLAVFLRFEHGLARRGAAPLLDLDALLAPGIRPGLAVVSIGFIGYGGWLFACALYLQTGLGDSPMISGFV
FAAYAFGFGISNVSWSKLPARLLRFTPAAALAVMVVANAAFGTGARQFGWLAALMVPLLFLAGASHGLTFGTTVSQMTQR
TAARHAPALSGLVSTSVQLSIVIGVAALGSVYLAIARPGDVPRSAQAISSVTFAIAALALVAIACSLRLATMRAAPPAAV
PAARPGGDAPRPH
>Mature_492_residues
SNSSTLAPGAAAPVRPHRRMLPLLLIGQGMAAMDTSIVNVAAPALRADLGISGALLQLVVAGYILAYAVFLITGARLGDD
HGYRRLFIHGLAIFTISSLACGLAPDTWLLIAGRLAQGVGAAMLVPQVMSLIQRSYEGRERARAIGYYSMILGLGSTAGQ
ALGGLIITANLGGLSWRPAFLINVPIGIVLLLYAPAVLPDLAGAKRRRLDLAGVALLTAAMLLVVVPLTFGHDAGWAAWV
WVALAAGVAGLAVFLRFEHGLARRGAAPLLDLDALLAPGIRPGLAVVSIGFIGYGGWLFACALYLQTGLGDSPMISGFVF
AAYAFGFGISNVSWSKLPARLLRFTPAAALAVMVVANAAFGTGARQFGWLAALMVPLLFLAGASHGLTFGTTVSQMTQRT
AARHAPALSGLVSTSVQLSIVIGVAALGSVYLAIARPGDVPRSAQAISSVTFAIAALALVAIACSLRLATMRAAPPAAVP
AARPGGDAPRPH

Specific function: Promotes the efflux of actinorhodin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=407, Percent_Identity=29.4840294840295, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI87081983, Length=306, Percent_Identity=31.6993464052288, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1788392, Length=420, Percent_Identity=28.3333333333333, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788710, Length=298, Percent_Identity=27.8523489932886, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1789042, Length=268, Percent_Identity=27.9850746268657, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324954, Length=412, Percent_Identity=24.7572815533981, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50669; Mature: 50537

Theoretical pI: Translated: 11.18; Mature: 11.18

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSSTLAPGAAAPVRPHRRMLPLLLIGQGMAAMDTSIVNVAAPALRADLGISGALLQLV
CCCCCCCCCCCCCCCCCHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VAGYILAYAVFLITGARLGDDHGYRRLFIHGLAIFTISSLACGLAPDTWLLIAGRLAQGV
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
GAAMLVPQVMSLIQRSYEGRERARAIGYYSMILGLGSTAGQALGGLIITANLGGLSWRPA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCEEEEEECCCCCCCCE
FLINVPIGIVLLLYAPAVLPDLAGAKRRRLDLAGVALLTAAMLLVVVPLTFGHDAGWAAW
EEEECHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
VWVALAAGVAGLAVFLRFEHGLARRGAAPLLDLDALLAPGIRPGLAVVSIGFIGYGGWLF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHH
ACALYLQTGLGDSPMISGFVFAAYAFGFGISNVSWSKLPARLLRFTPAAALAVMVVANAA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHH
FGTGARQFGWLAALMVPLLFLAGASHGLTFGTTVSQMTQRTAARHAPALSGLVSTSVQLS
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHCCHHH
IVIGVAALGSVYLAIARPGDVPRSAQAISSVTFAIAALALVAIACSLRLATMRAAPPAAV
HHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PAARPGGDAPRPH
CCCCCCCCCCCCC
>Mature Secondary Structure 
SNSSTLAPGAAAPVRPHRRMLPLLLIGQGMAAMDTSIVNVAAPALRADLGISGALLQLV
CCCCCCCCCCCCCCCCHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VAGYILAYAVFLITGARLGDDHGYRRLFIHGLAIFTISSLACGLAPDTWLLIAGRLAQGV
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
GAAMLVPQVMSLIQRSYEGRERARAIGYYSMILGLGSTAGQALGGLIITANLGGLSWRPA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCEEEEEECCCCCCCCE
FLINVPIGIVLLLYAPAVLPDLAGAKRRRLDLAGVALLTAAMLLVVVPLTFGHDAGWAAW
EEEECHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
VWVALAAGVAGLAVFLRFEHGLARRGAAPLLDLDALLAPGIRPGLAVVSIGFIGYGGWLF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHH
ACALYLQTGLGDSPMISGFVFAAYAFGFGISNVSWSKLPARLLRFTPAAALAVMVVANAA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHH
FGTGARQFGWLAALMVPLLFLAGASHGLTFGTTVSQMTQRTAARHAPALSGLVSTSVQLS
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHCCHHH
IVIGVAALGSVYLAIARPGDVPRSAQAISSVTFAIAALALVAIACSLRLATMRAAPPAAV
HHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PAARPGGDAPRPH
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1878971; 12000953 [H]