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The map label for this gene is glk [H]

Identifier: 238026460

GI number: 238026460

Start: 865274

End: 867190

Strand: Reverse

Name: glk [H]

Synonym: bglu_1g08040

Alternate gene names: 238026460

Gene position: 867190-865274 (Counterclockwise)

Preceding gene: 238026461

Following gene: 238026458

Centisome position: 22.2

GC content: 69.8

Gene sequence:

>1917_bases
ATGTCTACTGGTGCGCAAACTAAACCGGCAGCGGGCCAGCACGCCGACGGCCCGCGCCTGCTGGCCGACATCGGCGGCAC
CAACGCGCGCTTCGCGCTCGAGGTCGGCCCCGGCGAGATCACGCAGATCCGCGTCTACCCCGGCGCCGACTACCCGACGC
TGACCGACGCGATCCGCAAGTATCTGAAGGACGCCAAGATCGGCCGCGTCAACCACGCGGCGATCGCGATCGCGAACCCG
GTCGACGGCGACCAGGTGCGCATGACCAACCACGACTGGAGCTTCTCGATCGAGGCCACGCGCCGCGCGCTGGGCTTCGA
CACGCTGCTCGTGGTCAACGATTTCACCGCGCTCGCAATGGCGCTGCCCGGCCTGACCGACGCGCAGCGCCAGCAGGTGG
GCGGCGGCACGCGCCGCCAGAACAGCGTGATCGGCCTGATGGGGCCGGGCACCGGGCTCGGCGTGTCGGGCCTGATCCCG
GCCGACGACCGCTGGATCGCGCTCGGCAGCGAAGGCGGCCATGCCAGCTTCGCGCCGATGGACGAGCGCGAGGACCTGGT
CATGCAGTACGCGCGCAAGAAGTTTGCGCACGTCTCGTTCGAGCGCGTTTGCGCGGGGCCCGGCATCGAGCTCGTCTACC
GGGCACTGGCCGGGCGCGACAAGAAGCGCGTGCCGTCGGGCTTCGGCACGCCCGAGGTGGTCGAGCGGGCCCATGCGGGC
GATGCGCTCGCGCTCGAGGCGGTGGACGTGTTCTGCGCGATCCTCGGCACCTTCGCGGGCAACCTCGCCGTCACGCTCGG
CTCGCTCGGCGGCGTCTACATCGGCGGCGGCGTGGTGCTGAAGCTCGGCGAGCTGTTCCTGAAGTCGCGCTTTCGCGAGC
GCTTCGAGCAGAAAGGGCGCTTTTCCGAGTACCTCGCGAACGTGCCGACCTACCTGATCACCGCCGACTATCCGGCATTC
CTCGGCGTCTCGGCGATCCTCGCCGAGCAGTTGTCGAACCGCACGGGCGGCGCCTCGTCGGCCGTGTTCGAGCGGATCCG
CCAGATGCGCGACGCGCTCACGCCGGCCGAGCGCCGCGTGGCCGACCTCGCGCTGAACCATCCGCGCTCGATCATCAACG
ATCCGATCGTCGACATCGCGCGCAAGGCCGACGTGAGCCAGCCCACCGTGATCCGCTTCTGCCGCTCGCTCGGCTGCCAG
GGGCTGTCCGACTTCAAGCTCAAGCTCGCCACCGGCCTGACCGGCACCATCCCGATGAGCCACAGCCAGGTCCATCTCGG
CGACACGGCCACCGACTTCGGCGCGAAGGTGCTCGACAACACGGTCTCGGCGATCCTGCAGCTGCGCGAGCACCTGAACT
TCGAGCACGTCGCGCAGGCCATCGAGATCCTGAACGGCGCGCGGCGCATCGAGTTCTACGGGCTCGGCAACTCGAACATC
GTCGCGCAGGACGCGCATTACAAGTTCTTCCGCTTCGGGATTCCGACCATCGCCTACGGCGACCTCTACATGCAGGCCGC
CTCGGCCGCGCTGCTCGGCAAGGGCGACGTGATCGTGGCCGTCTCGAAGTCGGGCCGCGCGCCCGAGCTGCTGCGCGTGC
TCGAAGTGGCGATGCAGGCCGGCGCCAAGGTGATCGCGATCACCTCCAGCAACACGCCGCTCGCCAAGCGCGCGACGGTG
GCGCTGGAGACCGACCACATCGAGATGCGCGAGTCGCAGCTGTCGATGATCTCGCGGATCCTGCACCTCGTGATGATCGA
CATCCTGGCGGTGGGCGTCGCGATCCGGCGCGCCGCGCCGAACGCCGAGATCACCGAGGCGGTCGCGCAGGCGCGCTCGC
GTGCCGGCGACGACGAATCGGCCGACGTGCTCGACTGGCTCAGCCACGGCGCGGCGCCCGCGGCCGCGCGCGAATGA

Upstream 100 bases:

>100_bases
ATCGCCGGCGCCCGCAAGCGCGAGGTGCTGGAGGCCGCCGCCGCGGCGCCGCAGCGCAACGCGATTTCCCAACTGGCCAA
CGACAAGGGGACCAAGCTCG

Downstream 100 bases:

>100_bases
AGCGGCGCGCGCCGCCGCCGGGTCGCTGAAGCGGCGCCGGGCCGGCACCGCGCGGGTCGATCCCAAGCCAAGACAAGCCC
GGGCCGGCGCTTTCGGGGCG

Product: bifunctional glucokinase/RpiR family transcriptional regulator

Products: NA

Alternate protein names: Glucokinase; Glucose kinase; Putative HTH-type transcriptional regulator [H]

Number of amino acids: Translated: 638; Mature: 637

Protein sequence:

>638_residues
MSTGAQTKPAAGQHADGPRLLADIGGTNARFALEVGPGEITQIRVYPGADYPTLTDAIRKYLKDAKIGRVNHAAIAIANP
VDGDQVRMTNHDWSFSIEATRRALGFDTLLVVNDFTALAMALPGLTDAQRQQVGGGTRRQNSVIGLMGPGTGLGVSGLIP
ADDRWIALGSEGGHASFAPMDEREDLVMQYARKKFAHVSFERVCAGPGIELVYRALAGRDKKRVPSGFGTPEVVERAHAG
DALALEAVDVFCAILGTFAGNLAVTLGSLGGVYIGGGVVLKLGELFLKSRFRERFEQKGRFSEYLANVPTYLITADYPAF
LGVSAILAEQLSNRTGGASSAVFERIRQMRDALTPAERRVADLALNHPRSIINDPIVDIARKADVSQPTVIRFCRSLGCQ
GLSDFKLKLATGLTGTIPMSHSQVHLGDTATDFGAKVLDNTVSAILQLREHLNFEHVAQAIEILNGARRIEFYGLGNSNI
VAQDAHYKFFRFGIPTIAYGDLYMQAASAALLGKGDVIVAVSKSGRAPELLRVLEVAMQAGAKVIAITSSNTPLAKRATV
ALETDHIEMRESQLSMISRILHLVMIDILAVGVAIRRAAPNAEITEAVAQARSRAGDDESADVLDWLSHGAAPAAARE

Sequences:

>Translated_638_residues
MSTGAQTKPAAGQHADGPRLLADIGGTNARFALEVGPGEITQIRVYPGADYPTLTDAIRKYLKDAKIGRVNHAAIAIANP
VDGDQVRMTNHDWSFSIEATRRALGFDTLLVVNDFTALAMALPGLTDAQRQQVGGGTRRQNSVIGLMGPGTGLGVSGLIP
ADDRWIALGSEGGHASFAPMDEREDLVMQYARKKFAHVSFERVCAGPGIELVYRALAGRDKKRVPSGFGTPEVVERAHAG
DALALEAVDVFCAILGTFAGNLAVTLGSLGGVYIGGGVVLKLGELFLKSRFRERFEQKGRFSEYLANVPTYLITADYPAF
LGVSAILAEQLSNRTGGASSAVFERIRQMRDALTPAERRVADLALNHPRSIINDPIVDIARKADVSQPTVIRFCRSLGCQ
GLSDFKLKLATGLTGTIPMSHSQVHLGDTATDFGAKVLDNTVSAILQLREHLNFEHVAQAIEILNGARRIEFYGLGNSNI
VAQDAHYKFFRFGIPTIAYGDLYMQAASAALLGKGDVIVAVSKSGRAPELLRVLEVAMQAGAKVIAITSSNTPLAKRATV
ALETDHIEMRESQLSMISRILHLVMIDILAVGVAIRRAAPNAEITEAVAQARSRAGDDESADVLDWLSHGAAPAAARE
>Mature_637_residues
STGAQTKPAAGQHADGPRLLADIGGTNARFALEVGPGEITQIRVYPGADYPTLTDAIRKYLKDAKIGRVNHAAIAIANPV
DGDQVRMTNHDWSFSIEATRRALGFDTLLVVNDFTALAMALPGLTDAQRQQVGGGTRRQNSVIGLMGPGTGLGVSGLIPA
DDRWIALGSEGGHASFAPMDEREDLVMQYARKKFAHVSFERVCAGPGIELVYRALAGRDKKRVPSGFGTPEVVERAHAGD
ALALEAVDVFCAILGTFAGNLAVTLGSLGGVYIGGGVVLKLGELFLKSRFRERFEQKGRFSEYLANVPTYLITADYPAFL
GVSAILAEQLSNRTGGASSAVFERIRQMRDALTPAERRVADLALNHPRSIINDPIVDIARKADVSQPTVIRFCRSLGCQG
LSDFKLKLATGLTGTIPMSHSQVHLGDTATDFGAKVLDNTVSAILQLREHLNFEHVAQAIEILNGARRIEFYGLGNSNIV
AQDAHYKFFRFGIPTIAYGDLYMQAASAALLGKGDVIVAVSKSGRAPELLRVLEVAMQAGAKVIAITSSNTPLAKRATVA
LETDHIEMRESQLSMISRILHLVMIDILAVGVAIRRAAPNAEITEAVAQARSRAGDDESADVLDWLSHGAAPAAARE

Specific function: Not Highly Important In E.Coli As Glucose Is Transported Into The Cell By The Pts System Already As Glucose 6-Phosphate. [C]

COG id: COG0837

COG function: function code G; Glucokinase

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SIS domain [H]

Homologues:

Organism=Escherichia coli, GI1788732, Length=316, Percent_Identity=45.253164556962, Blast_Score=278, Evalue=6e-76,
Organism=Escherichia coli, GI1788159, Length=275, Percent_Identity=35.6363636363636, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI87082369, Length=268, Percent_Identity=28.7313432835821, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1788767, Length=249, Percent_Identity=27.3092369477912, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082130, Length=226, Percent_Identity=28.3185840707965, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003836
- InterPro:   IPR000843
- InterPro:   IPR000281
- InterPro:   IPR001347
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02685 Glucokinase; PF01418 HTH_6; PF01380 SIS [H]

EC number: =2.7.1.2 [H]

Molecular weight: Translated: 68193; Mature: 68062

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: PS00356 HTH_LACI_1 ; PS51071 HTH_RPIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTGAQTKPAAGQHADGPRLLADIGGTNARFALEVGPGEITQIRVYPGADYPTLTDAIRK
CCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCEEEEEEECCCCCCHHHHHHHH
YLKDAKIGRVNHAAIAIANPVDGDQVRMTNHDWSFSIEATRRALGFDTLLVVNDFTALAM
HHHHCCCCCCCCEEEEEECCCCCCEEEEECCCCEEEHHHHHHHHCCCEEEEHHHHHHHHH
ALPGLTDAQRQQVGGGTRRQNSVIGLMGPGTGLGVSGLIPADDRWIALGSEGGHASFAPM
HCCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCC
DEREDLVMQYARKKFAHVSFERVCAGPGIELVYRALAGRDKKRVPSGFGTPEVVERAHAG
CCHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHCCCHHCCCCCCCCHHHHHHHCCC
DALALEAVDVFCAILGTFAGNLAVTLGSLGGVYIGGGVVLKLGELFLKSRFRERFEQKGR
CHHHHHHHHHHHHHHHHHHCHHEEEHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCC
FSEYLANVPTYLITADYPAFLGVSAILAEQLSNRTGGASSAVFERIRQMRDALTPAERRV
HHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHH
ADLALNHPRSIINDPIVDIARKADVSQPTVIRFCRSLGCQGLSDFKLKLATGLTGTIPMS
HHHHHCCCHHHHCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCC
HSQVHLGDTATDFGAKVLDNTVSAILQLREHLNFEHVAQAIEILNGARRIEFYGLGNSNI
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCCE
VAQDAHYKFFRFGIPTIAYGDLYMQAASAALLGKGDVIVAVSKSGRAPELLRVLEVAMQA
EEECCCCEEEEECCCCEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHC
GAKVIAITSSNTPLAKRATVALETDHIEMRESQLSMISRILHLVMIDILAVGVAIRRAAP
CCEEEEEECCCCCCCHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NAEITEAVAQARSRAGDDESADVLDWLSHGAAPAAARE
CHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
STGAQTKPAAGQHADGPRLLADIGGTNARFALEVGPGEITQIRVYPGADYPTLTDAIRK
CCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCEEEEEEECCCCCCHHHHHHHH
YLKDAKIGRVNHAAIAIANPVDGDQVRMTNHDWSFSIEATRRALGFDTLLVVNDFTALAM
HHHHCCCCCCCCEEEEEECCCCCCEEEEECCCCEEEHHHHHHHHCCCEEEEHHHHHHHHH
ALPGLTDAQRQQVGGGTRRQNSVIGLMGPGTGLGVSGLIPADDRWIALGSEGGHASFAPM
HCCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCC
DEREDLVMQYARKKFAHVSFERVCAGPGIELVYRALAGRDKKRVPSGFGTPEVVERAHAG
CCHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHCCCHHCCCCCCCCHHHHHHHCCC
DALALEAVDVFCAILGTFAGNLAVTLGSLGGVYIGGGVVLKLGELFLKSRFRERFEQKGR
CHHHHHHHHHHHHHHHHHHCHHEEEHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCC
FSEYLANVPTYLITADYPAFLGVSAILAEQLSNRTGGASSAVFERIRQMRDALTPAERRV
HHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHH
ADLALNHPRSIINDPIVDIARKADVSQPTVIRFCRSLGCQGLSDFKLKLATGLTGTIPMS
HHHHHCCCHHHHCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCC
HSQVHLGDTATDFGAKVLDNTVSAILQLREHLNFEHVAQAIEILNGARRIEFYGLGNSNI
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCCE
VAQDAHYKFFRFGIPTIAYGDLYMQAASAALLGKGDVIVAVSKSGRAPELLRVLEVAMQA
EEECCCCEEEEECCCCEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHC
GAKVIAITSSNTPLAKRATVALETDHIEMRESQLSMISRILHLVMIDILAVGVAIRRAAP
CCEEEEEECCCCCCCHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NAEITEAVAQARSRAGDDESADVLDWLSHGAAPAAARE
CHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA