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The map label for this gene is bcr [H]

Identifier: 238026459

GI number: 238026459

Start: 863472

End: 865094

Strand: Direct

Name: bcr [H]

Synonym: bglu_1g08030

Alternate gene names: 238026459

Gene position: 863472-865094 (Clockwise)

Preceding gene: 238026455

Following gene: 238026463

Centisome position: 22.1

GC content: 71.84

Gene sequence:

>1623_bases
ATGCTGCGCGAGAAACCGCAGCCCCAGACGTCGCGCACGGCCTCGCCGGCGCGCACGCGCGCGGCGAGTTCGGGCAACTC
GGGCCACGGCCATTTCCGGGGGCTCCAGATCAGCAGGAAGGCGGTCATGTCGGTCGGGTCAACGGGGTTCTCGGGCGGGG
GGCGCGAATTCGGGGGCAGCGCCTGCGCGAGCTTACCCGATCGCCGCCGGCGCCGCGTCGATGCGCGTGCACCGGCCCGG
CGGCGGGGCCGGACGAACGATTTCGGACAAGCGCGTACAATTACCGCTTTGCGGCGGCCGTCCGGCCGCCCGTCCCGCCC
GAGCCGGGCCGCGCCGAATCGCGTCGAACCGCCTCGCGGCGAAGCGAGCCGAAGCGGGCGAAGTCCCGGCCGCTCCCGAG
AATTTCCCATGTCGAATCTGGTACGGCGCCGGCCCGACGCCCGGCTCATCCTGTTGCTCGGCGCGCTCGCCGCCTGTGGT
CCGATCGGCACCGACATGTACCTGCCGAGCATGCCGTCGGTGGCCGAGAGCCTCGGCACCAGCGCGGCGGCCGTGCAATG
GACGCTCACCAGCTTCATTGCGGGCTTCTCGCTCGGCATGCTGCTGTACGGCCCGCTGTCCGACGCGCTCGGGCGCCGGC
CGGTGCTGCTCGGCGGCATCGTGCTGTTCACGGTGGCGGGCGTCGGCTGCTTCGTGGCGCCGAGCATCGGCGCGCTGATC
GTGCTGCGCTTCCTGCAGGCGCTCGGCGCGGGCGCGGCCTCGGTGTTGTCGCGCGCCATCGCGCGCGACGCGCACGAACC
GGCCGACGCGGCCCGCGTGCTGTCGATGGTGGCCATCGTCACCTCGGTCGGCCCGCTGCTCGCGCCGCTGCTGGGCGGGC
AGATCCTGCGCTTCGCCGGCTGGCGCACGGTGTTCGTGGTGCTGGCGGTGTTCGGCCTCGGCTGCGCGATCGCCGCGTTC
CTGAAGGTGCCGGAGACCTGGCCTAAGGAGCGGCGCCGGTCTTCGGCGATCCTGGGCTCGTTCGCGTCCTACGGGCGCAT
CATCGGCGATCCGGTGGCCTGGGGGCACATGCTCTGCGGCGGCATGGCGTTCGCCTCGATGATCACGTACATCACCGCCA
CGCCGTTCGTCTACATCGAGTATTTCCACGTCTCGGCGCAGCACTACGGCCTGCTGTTCGGCCTGAACATCATCGGCATC
ATCATCGGCAACTTCATCAACACGCGCGTGGTCGGCCGGCTCGGCTCGCTGCGGCTGATCTCGGCGGCCTCGCTGGTGAG
CAGCATCGCCTCGTTCGCGGTGGCGTTCTTCGCGCTGACGGGGATCGGCGGGCTCTGGTCGATCGTCGCGGGGCTGTTCT
TCGTGGTCGGCGTGATCGGCCTGCTGTCGGCCAACTGCTCGACCGATCTGATGCACCGCTATCCCCACAACGCGGGGGCC
GCGGCCGCCGTGTTCGGGGCGATGCAGCTCGGGCTGGGCGCCGTGGCGAGCGCGGTGGTGGGGCTGCTGGCCGACGGCAC
GCCGTTCGGCATGGGCGTGACCATCGCGATCACGGGCGCGCTGTGCCTGATGGGCCGCAGGCTCGTGCTGCGCTGGCACG
GCCGGCCGGTGCGCGGCGACTGA

Upstream 100 bases:

>100_bases
GTCGGCGACCTCGTAGGGCGCGCGCGTCACGTAGCCGGAGCCGAACACGCCCCTGGGCTCCTGGGCGACGCGGTGCAGGA
ACACGCGATCGCCGGGCAGG

Downstream 100 bases:

>100_bases
GGCCGCGCACCGCGGGACGCACGGGAAGGCGCCCGCGGCCGAAGCCGCAAGGCGAAGCGCGAAACGACACCGGGGCCGGC
GCCCCGAAAGCGCCGGCCCG

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MLREKPQPQTSRTASPARTRAASSGNSGHGHFRGLQISRKAVMSVGSTGFSGGGREFGGSACASLPDRRRRRVDARAPAR
RRGRTNDFGQARTITALRRPSGRPSRPSRAAPNRVEPPRGEASRSGRSPGRSREFPMSNLVRRRPDARLILLLGALAACG
PIGTDMYLPSMPSVAESLGTSAAAVQWTLTSFIAGFSLGMLLYGPLSDALGRRPVLLGGIVLFTVAGVGCFVAPSIGALI
VLRFLQALGAGAASVLSRAIARDAHEPADAARVLSMVAIVTSVGPLLAPLLGGQILRFAGWRTVFVVLAVFGLGCAIAAF
LKVPETWPKERRRSSAILGSFASYGRIIGDPVAWGHMLCGGMAFASMITYITATPFVYIEYFHVSAQHYGLLFGLNIIGI
IIGNFINTRVVGRLGSLRLISAASLVSSIASFAVAFFALTGIGGLWSIVAGLFFVVGVIGLLSANCSTDLMHRYPHNAGA
AAAVFGAMQLGLGAVASAVVGLLADGTPFGMGVTIAITGALCLMGRRLVLRWHGRPVRGD

Sequences:

>Translated_540_residues
MLREKPQPQTSRTASPARTRAASSGNSGHGHFRGLQISRKAVMSVGSTGFSGGGREFGGSACASLPDRRRRRVDARAPAR
RRGRTNDFGQARTITALRRPSGRPSRPSRAAPNRVEPPRGEASRSGRSPGRSREFPMSNLVRRRPDARLILLLGALAACG
PIGTDMYLPSMPSVAESLGTSAAAVQWTLTSFIAGFSLGMLLYGPLSDALGRRPVLLGGIVLFTVAGVGCFVAPSIGALI
VLRFLQALGAGAASVLSRAIARDAHEPADAARVLSMVAIVTSVGPLLAPLLGGQILRFAGWRTVFVVLAVFGLGCAIAAF
LKVPETWPKERRRSSAILGSFASYGRIIGDPVAWGHMLCGGMAFASMITYITATPFVYIEYFHVSAQHYGLLFGLNIIGI
IIGNFINTRVVGRLGSLRLISAASLVSSIASFAVAFFALTGIGGLWSIVAGLFFVVGVIGLLSANCSTDLMHRYPHNAGA
AAAVFGAMQLGLGAVASAVVGLLADGTPFGMGVTIAITGALCLMGRRLVLRWHGRPVRGD
>Mature_540_residues
MLREKPQPQTSRTASPARTRAASSGNSGHGHFRGLQISRKAVMSVGSTGFSGGGREFGGSACASLPDRRRRRVDARAPAR
RRGRTNDFGQARTITALRRPSGRPSRPSRAAPNRVEPPRGEASRSGRSPGRSREFPMSNLVRRRPDARLILLLGALAACG
PIGTDMYLPSMPSVAESLGTSAAAVQWTLTSFIAGFSLGMLLYGPLSDALGRRPVLLGGIVLFTVAGVGCFVAPSIGALI
VLRFLQALGAGAASVLSRAIARDAHEPADAARVLSMVAIVTSVGPLLAPLLGGQILRFAGWRTVFVVLAVFGLGCAIAAF
LKVPETWPKERRRSSAILGSFASYGRIIGDPVAWGHMLCGGMAFASMITYITATPFVYIEYFHVSAQHYGLLFGLNIIGI
IIGNFINTRVVGRLGSLRLISAASLVSSIASFAVAFFALTGIGGLWSIVAGLFFVVGVIGLLSANCSTDLMHRYPHNAGA
AAAVFGAMQLGLGAVASAVVGLLADGTPFGMGVTIAITGALCLMGRRLVLRWHGRPVRGD

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=390, Percent_Identity=34.3589743589744, Blast_Score=238, Evalue=5e-64,
Organism=Escherichia coli, GI48994889, Length=375, Percent_Identity=30.6666666666667, Blast_Score=135, Evalue=9e-33,
Organism=Escherichia coli, GI1790146, Length=257, Percent_Identity=28.7937743190661, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87082312, Length=344, Percent_Identity=27.3255813953488, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787065, Length=260, Percent_Identity=30, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1790794, Length=165, Percent_Identity=24.2424242424242, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1790195, Length=164, Percent_Identity=28.0487804878049, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1789042, Length=138, Percent_Identity=28.2608695652174, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324264, Length=178, Percent_Identity=24.7191011235955, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6319480, Length=384, Percent_Identity=21.09375, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6319657, Length=213, Percent_Identity=24.4131455399061, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6319517, Length=285, Percent_Identity=24.5614035087719, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56573; Mature: 56573

Theoretical pI: Translated: 12.13; Mature: 12.13

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLREKPQPQTSRTASPARTRAASSGNSGHGHFRGLQISRKAVMSVGSTGFSGGGREFGGS
CCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCH
ACASLPDRRRRRVDARAPARRRGRTNDFGQARTITALRRPSGRPSRPSRAAPNRVEPPRG
HHHCCCHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
EASRSGRSPGRSREFPMSNLVRRRPDARLILLLGALAACGPIGTDMYLPSMPSVAESLGT
CCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCC
SAAAVQWTLTSFIAGFSLGMLLYGPLSDALGRRPVLLGGIVLFTVAGVGCFVAPSIGALI
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VLRFLQALGAGAASVLSRAIARDAHEPADAARVLSMVAIVTSVGPLLAPLLGGQILRFAG
HHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WRTVFVVLAVFGLGCAIAAFLKVPETWPKERRRSSAILGSFASYGRIIGDPVAWGHMLCG
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHHH
GMAFASMITYITATPFVYIEYFHVSAQHYGLLFGLNIIGIIIGNFINTRVVGRLGSLRLI
HHHHHHHHHHHHHCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SAASLVSSIASFAVAFFALTGIGGLWSIVAGLFFVVGVIGLLSANCSTDLMHRYPHNAGA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCH
AAAVFGAMQLGLGAVASAVVGLLADGTPFGMGVTIAITGALCLMGRRLVLRWHGRPVRGD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHEEEECCCCCCCC
>Mature Secondary Structure
MLREKPQPQTSRTASPARTRAASSGNSGHGHFRGLQISRKAVMSVGSTGFSGGGREFGGS
CCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCH
ACASLPDRRRRRVDARAPARRRGRTNDFGQARTITALRRPSGRPSRPSRAAPNRVEPPRG
HHHCCCHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
EASRSGRSPGRSREFPMSNLVRRRPDARLILLLGALAACGPIGTDMYLPSMPSVAESLGT
CCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCC
SAAAVQWTLTSFIAGFSLGMLLYGPLSDALGRRPVLLGGIVLFTVAGVGCFVAPSIGALI
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VLRFLQALGAGAASVLSRAIARDAHEPADAARVLSMVAIVTSVGPLLAPLLGGQILRFAG
HHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WRTVFVVLAVFGLGCAIAAFLKVPETWPKERRRSSAILGSFASYGRIIGDPVAWGHMLCG
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHHH
GMAFASMITYITATPFVYIEYFHVSAQHYGLLFGLNIIGIIIGNFINTRVVGRLGSLRLI
HHHHHHHHHHHHHCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SAASLVSSIASFAVAFFALTGIGGLWSIVAGLFFVVGVIGLLSANCSTDLMHRYPHNAGA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCH
AAAVFGAMQLGLGAVASAVVGLLADGTPFGMGVTIAITGALCLMGRRLVLRWHGRPVRGD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]