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The map label for this gene is gcvA [H]

Identifier: 238025206

GI number: 238025206

Start: 2340042

End: 2340971

Strand: Direct

Name: gcvA [H]

Synonym: bglu_2g18770

Alternate gene names: 238025206

Gene position: 2340042-2340971 (Clockwise)

Preceding gene: 238025195

Following gene: 238025209

Centisome position: 82.76

GC content: 70.0

Gene sequence:

>930_bases
ATGCGCAAGTTCAAGATACCGAACATGGGCGCGCTGCTGGCATTCGAGGCGGCGTCGCGCCATGAGAGCTTCACCTACGC
GGCGCGCGAGCTGTTCCTGACCGAAAGCGCGATCTCGCGGCAGATCGCGACGCTGGAGGCCGGCCTCGGCGTGCGCCTGT
TCGTGCGCGCCAAGCAGCGCGTGGTGCTCACGCGCGCGGGGCGCCTGTATAGCACCCAGGTGCGGCGCGCGCTCGAGCAT
CTCGACCGCGACACGCTCTCGATCATCGCGCACGGCAGCGGCGGCGGCTATCTGGAGCTGGCGGTGCTGCCCACCTTCGC
CTCGCAATGGCTGATTCCGCGCCTGAAGGACTTCTACGACGGCACCCCCGACGTGCGCGTGAACATGGGGGCGCGCACCG
GCATCTTCTCGTTCGCGGAATCGCATTTCGAGGCGGCGATCCATTACGGCAAGCCGACCTGGCCCGGCGCGTCGGCCGAC
TACCTGTTCGGCGAGGAAGTCGTGCCGGTCTGCGCGCCGGCGCTGCTGAAGCGGCCCGTGCGCAAGCCGCACGAGCTGCT
GGCCTACCCGCTGCTGCATTCCACCACGCGTCCCGACGGCTGGGCACGCTGGTTCGCCTCGCTCGGCGTGGACCAACCCG
GCACCATGCAGGGCGACCGCTACGAGCTGCATACCATGCTGATCAGCGCCGCGGCAGCGGGGCTCGGCATCGCCCTGGTG
CCGAAGTTCTTCGTCGACGGCCGCGAGCGCCAGCTCGGCATCGTGGTGCCCTACGTGGCACCGTCGGCGGCGGCCGCACG
GCTGGCCGCCGCCGACGACGTGGCCTATTACCTCGTGTATCCGACCGAGCTGAGCCATAGCCGGCCGCTCGACGCGTTTC
GCGGCTGGCTGCTGGCGCAGGCACACGCCTACGGCGGCAAGCCGGACTAG

Upstream 100 bases:

>100_bases
TCGCAGGCGCCGCCTCGCTTGAGCCCGCTGCATTATCGAAACGATCGGGGCCGGCGTAAAGCGAGATAAAATCGGGACTT
GATGCGTTTTTTGAATCATC

Downstream 100 bases:

>100_bases
GCGCGCGGCGGGCATCGGGCAGGCCTGCGCCGGCCCGGTGCGGCGGGCGGGCGTGGCCACGCGGGGCGGCCACACCCGCC
GGCTCACCGCGTCACGCGTA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MRKFKIPNMGALLAFEAASRHESFTYAARELFLTESAISRQIATLEAGLGVRLFVRAKQRVVLTRAGRLYSTQVRRALEH
LDRDTLSIIAHGSGGGYLELAVLPTFASQWLIPRLKDFYDGTPDVRVNMGARTGIFSFAESHFEAAIHYGKPTWPGASAD
YLFGEEVVPVCAPALLKRPVRKPHELLAYPLLHSTTRPDGWARWFASLGVDQPGTMQGDRYELHTMLISAAAAGLGIALV
PKFFVDGRERQLGIVVPYVAPSAAAARLAAADDVAYYLVYPTELSHSRPLDAFRGWLLAQAHAYGGKPD

Sequences:

>Translated_309_residues
MRKFKIPNMGALLAFEAASRHESFTYAARELFLTESAISRQIATLEAGLGVRLFVRAKQRVVLTRAGRLYSTQVRRALEH
LDRDTLSIIAHGSGGGYLELAVLPTFASQWLIPRLKDFYDGTPDVRVNMGARTGIFSFAESHFEAAIHYGKPTWPGASAD
YLFGEEVVPVCAPALLKRPVRKPHELLAYPLLHSTTRPDGWARWFASLGVDQPGTMQGDRYELHTMLISAAAAGLGIALV
PKFFVDGRERQLGIVVPYVAPSAAAARLAAADDVAYYLVYPTELSHSRPLDAFRGWLLAQAHAYGGKPD
>Mature_309_residues
MRKFKIPNMGALLAFEAASRHESFTYAARELFLTESAISRQIATLEAGLGVRLFVRAKQRVVLTRAGRLYSTQVRRALEH
LDRDTLSIIAHGSGGGYLELAVLPTFASQWLIPRLKDFYDGTPDVRVNMGARTGIFSFAESHFEAAIHYGKPTWPGASAD
YLFGEEVVPVCAPALLKRPVRKPHELLAYPLLHSTTRPDGWARWFASLGVDQPGTMQGDRYELHTMLISAAAAGLGIALV
PKFFVDGRERQLGIVVPYVAPSAAAARLAAADDVAYYLVYPTELSHSRPLDAFRGWLLAQAHAYGGKPD

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=310, Percent_Identity=33.2258064516129, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1786448, Length=292, Percent_Identity=32.5342465753425, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1788706, Length=258, Percent_Identity=28.6821705426357, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI145693193, Length=309, Percent_Identity=26.8608414239482, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI157672245, Length=118, Percent_Identity=37.2881355932203, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790262, Length=273, Percent_Identity=29.3040293040293, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33965; Mature: 33965

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKFKIPNMGALLAFEAASRHESFTYAARELFLTESAISRQIATLEAGLGVRLFVRAKQR
CCCCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHE
VVLTRAGRLYSTQVRRALEHLDRDTLSIIAHGSGGGYLELAVLPTFASQWLIPRLKDFYD
EEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHC
GTPDVRVNMGARTGIFSFAESHFEAAIHYGKPTWPGASADYLFGEEVVPVCAPALLKRPV
CCCCEEEECCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
RKPHELLAYPLLHSTTRPDGWARWFASLGVDQPGTMQGDRYELHTMLISAAAAGLGIALV
CCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHH
PKFFVDGRERQLGIVVPYVAPSAAAARLAAADDVAYYLVYPTELSHSRPLDAFRGWLLAQ
HHHHHCCCCCEEEEEEEECCCCHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHH
AHAYGGKPD
HHCCCCCCC
>Mature Secondary Structure
MRKFKIPNMGALLAFEAASRHESFTYAARELFLTESAISRQIATLEAGLGVRLFVRAKQR
CCCCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHE
VVLTRAGRLYSTQVRRALEHLDRDTLSIIAHGSGGGYLELAVLPTFASQWLIPRLKDFYD
EEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHC
GTPDVRVNMGARTGIFSFAESHFEAAIHYGKPTWPGASADYLFGEEVVPVCAPALLKRPV
CCCCEEEECCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
RKPHELLAYPLLHSTTRPDGWARWFASLGVDQPGTMQGDRYELHTMLISAAAAGLGIALV
CCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHH
PKFFVDGRERQLGIVVPYVAPSAAAARLAAADDVAYYLVYPTELSHSRPLDAFRGWLLAQ
HHHHHCCCCCEEEEEEEECCCCHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHH
AHAYGGKPD
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]