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The map label for this gene is oprM [H]

Identifier: 238024427

GI number: 238024427

Start: 1307418

End: 1308881

Strand: Direct

Name: oprM [H]

Synonym: bglu_2g10220

Alternate gene names: 238024427

Gene position: 1307418-1308881 (Clockwise)

Preceding gene: 238024426

Following gene: 238024428

Centisome position: 46.24

GC content: 70.42

Gene sequence:

>1464_bases
ATGCGCGCACTCATGGTTCTACTGTTGCTGACGGTGTCGGCATGCTCGCTGGACCCCGCGCTGGTCAAACCCGAGCCGCC
GGTGCCCGCCACCTTCCCGACCGAGGCCGGCCGCGCCACCCAGGCGCATGCCGCCGATCTCGGCTGGCGCACGATGTTCC
GCGACCCGCGCCTGCAACGCCTGATCGAACTGGGGCTGGGCCACAACCGCGACCTGCGCCTGGCGGTTCTGAACGTCGAG
GCCGTCCGTGCCCAATACGGCATTCAGCGCGCGGCCCGGCTGCCGTCGATCGAGGCGACCGCCAGCGCCACTCGCCAGCG
CACGGTGGCCGGCAGCGAGCTCGGCCCGGCCGTGCCCGCGGCAGTCCAGGAGCAGTACGTCCTCAGTCTCGGCCTCAATG
CCTTCGAGGTCGATCTGTTCGGCCGCGTGCGCTCCTTGTCCGAGGCCGCCTTCGCCCGCTACCTTGCCAGCGAGCAAGGG
CGCCGAGCGACGCAGATCGCCTTGGTCGGCGCGATCGCCGATGCCTATTTCGCCGAGCGTCTGGCGCAAGCGCAGCACCG
CCTGGCCGAGCGCACGCTGGCGGACTGGCGCCAATCGCTGGACCTGGCGCGCAAATTGAAAGCGGCCAACCAGAGCAGCG
GCCTGGATGTGGCCCAGGCCGAAGCTCAGGTCGCCAGCGCCCAAGCCGACCTGGAAGCGCGCGGGCGCGCCGTGGCACAG
TCGCGCAACGCGCTGCGGCTGCTGATCGGCACCGACCTTCCGGCGAATCTGCCGGCATCGATTCCCTTGGACGCGCAACC
GGTGGTGACCCGGTTGCCGGCGGGGCTGCCGTCCGATCTGTTGCTTCGCCGTCCCGACATTCTGCAAGCCGAGCAAAGCC
TGGTTGCCGCCAATGCCGAGATCGGCGCCGCGCGCGCCGCGTTCTTCCCGCGCCTGTCGCTGACCGCCGCGCTCGGTTAT
GCGAGTCCGGCCATGCACGGGCTGTTCGACGGCGGCAGCCGGGCCTGGAGCTTTGCGCCGCAGATCACCGTGCCGATCTT
CCAGGCAGGAAGGCTGCGTGCCGAACTGCGGCTGGCGCAACTGCGTAAGTCCAGTGCCGTTGCCGAGTACGAAAAGGCCA
TCCAGGCCGCGTTCCGCGAAGTGGCCGATGGCCTCGCCGGTCAAGAAACCTATGGACGGCAGATCGAGGCGCAGGCGCGC
GTGGTATCGAGCGCCGAGCGCCGCATGGAACTGTCCAAGCTGCGCTACCTCGCAGGCGTGGAGGGGCGGCTGGAGCTGCT
CGATTCGCAGCGGCAGCTCTACGCGGCACGGCAGGCACTGCTGGAACTTCGCCGCAGCGCGCTCGGCAACGCCGTCTCGC
TCTACAAGGCGCTCGGCGGCGGATTGACGGACCGGGACGCGAGCCCGGCCGACGGCACGGCGCTGCAAGGGCCCGTCGAC
GCGACCGTCGACGCGCGGGGATGA

Upstream 100 bases:

>100_bases
TTCTTCGTGTTCGTGATGGGGTTCCAGGAACGCTTCGTGACCTGGCGGGCAAACCGCCGGGCGGCCCGCCCGTCCCACCA
CCAGCAACAGGATTGATGTC

Downstream 100 bases:

>100_bases
CGCGGCAGGGGCGCGCCACCGGCGATCGCGCCGGACATGCGTGACGGCAGGCCCTCGCTGCGCGCTTTCCGCTTGGCCTC
CCACGGATTGGTGAAATGGC

Product: type I secretion outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MRALMVLLLLTVSACSLDPALVKPEPPVPATFPTEAGRATQAHAADLGWRTMFRDPRLQRLIELGLGHNRDLRLAVLNVE
AVRAQYGIQRAARLPSIEATASATRQRTVAGSELGPAVPAAVQEQYVLSLGLNAFEVDLFGRVRSLSEAAFARYLASEQG
RRATQIALVGAIADAYFAERLAQAQHRLAERTLADWRQSLDLARKLKAANQSSGLDVAQAEAQVASAQADLEARGRAVAQ
SRNALRLLIGTDLPANLPASIPLDAQPVVTRLPAGLPSDLLLRRPDILQAEQSLVAANAEIGAARAAFFPRLSLTAALGY
ASPAMHGLFDGGSRAWSFAPQITVPIFQAGRLRAELRLAQLRKSSAVAEYEKAIQAAFREVADGLAGQETYGRQIEAQAR
VVSSAERRMELSKLRYLAGVEGRLELLDSQRQLYAARQALLELRRSALGNAVSLYKALGGGLTDRDASPADGTALQGPVD
ATVDARG

Sequences:

>Translated_487_residues
MRALMVLLLLTVSACSLDPALVKPEPPVPATFPTEAGRATQAHAADLGWRTMFRDPRLQRLIELGLGHNRDLRLAVLNVE
AVRAQYGIQRAARLPSIEATASATRQRTVAGSELGPAVPAAVQEQYVLSLGLNAFEVDLFGRVRSLSEAAFARYLASEQG
RRATQIALVGAIADAYFAERLAQAQHRLAERTLADWRQSLDLARKLKAANQSSGLDVAQAEAQVASAQADLEARGRAVAQ
SRNALRLLIGTDLPANLPASIPLDAQPVVTRLPAGLPSDLLLRRPDILQAEQSLVAANAEIGAARAAFFPRLSLTAALGY
ASPAMHGLFDGGSRAWSFAPQITVPIFQAGRLRAELRLAQLRKSSAVAEYEKAIQAAFREVADGLAGQETYGRQIEAQAR
VVSSAERRMELSKLRYLAGVEGRLELLDSQRQLYAARQALLELRRSALGNAVSLYKALGGGLTDRDASPADGTALQGPVD
ATVDARG
>Mature_487_residues
MRALMVLLLLTVSACSLDPALVKPEPPVPATFPTEAGRATQAHAADLGWRTMFRDPRLQRLIELGLGHNRDLRLAVLNVE
AVRAQYGIQRAARLPSIEATASATRQRTVAGSELGPAVPAAVQEQYVLSLGLNAFEVDLFGRVRSLSEAAFARYLASEQG
RRATQIALVGAIADAYFAERLAQAQHRLAERTLADWRQSLDLARKLKAANQSSGLDVAQAEAQVASAQADLEARGRAVAQ
SRNALRLLIGTDLPANLPASIPLDAQPVVTRLPAGLPSDLLLRRPDILQAEQSLVAANAEIGAARAAFFPRLSLTAALGY
ASPAMHGLFDGGSRAWSFAPQITVPIFQAGRLRAELRLAQLRKSSAVAEYEKAIQAAFREVADGLAGQETYGRQIEAQAR
VVSSAERRMELSKLRYLAGVEGRLELLDSQRQLYAARQALLELRRSALGNAVSLYKALGGGLTDRDASPADGTALQGPVD
ATVDARG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=456, Percent_Identity=40.7894736842105, Blast_Score=324, Evalue=8e-90,
Organism=Escherichia coli, GI2367348, Length=470, Percent_Identity=28.0851063829787, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52134; Mature: 52134

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALMVLLLLTVSACSLDPALVKPEPPVPATFPTEAGRATQAHAADLGWRTMFRDPRLQR
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHCCHHHHH
LIELGLGHNRDLRLAVLNVEAVRAQYGIQRAARLPSIEATASATRQRTVAGSELGPAVPA
HHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHH
AVQEQYVLSLGLNAFEVDLFGRVRSLSEAAFARYLASEQGRRATQIALVGAIADAYFAER
HHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LAQAQHRLAERTLADWRQSLDLARKLKAANQSSGLDVAQAEAQVASAQADLEARGRAVAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SRNALRLLIGTDLPANLPASIPLDAQPVVTRLPAGLPSDLLLRRPDILQAEQSLVAANAE
CCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHCCH
IGAARAAFFPRLSLTAALGYASPAMHGLFDGGSRAWSFAPQITVPIFQAGRLRAELRLAQ
HHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCEECCCCEEEHHHHCCHHHHHHHHHH
LRKSSAVAEYEKAIQAAFREVADGLAGQETYGRQIEAQARVVSSAERRMELSKLRYLAGV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EGRLELLDSQRQLYAARQALLELRRSALGNAVSLYKALGGGLTDRDASPADGTALQGPVD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
ATVDARG
CCCCCCC
>Mature Secondary Structure
MRALMVLLLLTVSACSLDPALVKPEPPVPATFPTEAGRATQAHAADLGWRTMFRDPRLQR
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHCCHHHHH
LIELGLGHNRDLRLAVLNVEAVRAQYGIQRAARLPSIEATASATRQRTVAGSELGPAVPA
HHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHH
AVQEQYVLSLGLNAFEVDLFGRVRSLSEAAFARYLASEQGRRATQIALVGAIADAYFAER
HHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LAQAQHRLAERTLADWRQSLDLARKLKAANQSSGLDVAQAEAQVASAQADLEARGRAVAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SRNALRLLIGTDLPANLPASIPLDAQPVVTRLPAGLPSDLLLRRPDILQAEQSLVAANAE
CCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHCCH
IGAARAAFFPRLSLTAALGYASPAMHGLFDGGSRAWSFAPQITVPIFQAGRLRAELRLAQ
HHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCEECCCCEEEHHHHCCHHHHHHHHHH
LRKSSAVAEYEKAIQAAFREVADGLAGQETYGRQIEAQARVVSSAERRMELSKLRYLAGV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EGRLELLDSQRQLYAARQALLELRRSALGNAVSLYKALGGGLTDRDASPADGTALQGPVD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
ATVDARG
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]