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The map label for this gene is mexA [H]

Identifier: 238024426

GI number: 238024426

Start: 1303089

End: 1304249

Strand: Direct

Name: mexA [H]

Synonym: bglu_2g10200

Alternate gene names: 238024426

Gene position: 1303089-1304249 (Clockwise)

Preceding gene: 238024423

Following gene: 238024427

Centisome position: 46.09

GC content: 71.15

Gene sequence:

>1161_bases
ATGAAGATCGAATCACCGTGCCGCCGCGCAGGCCTCTCTGTGCTGTTGACGGTCATGCTGACGGCTTGCGGGCAATCCGA
GCCGCAGGAAAGCGCTCCGCCCGCGCCCCAGGTCTCGGCGACGACCTTGGTGCCTGCTCGGCTGGAACTGAGCGACGAGC
TGCCCGGGCGCGTCGCCGCCTTGCGCGTCGCCGAGATTCGGCCGCAAGTCAGCGGCATCGTGCAGCGCCGGCTGTTCGAG
CAAGGTAGCGAAGTGCGCGCCGGCCAGCCCTTATTCCAGATCAATCCCGCGCCGTTCCGGGCCGACGCGGACACCGCCGC
CGCCGCCCTGCAGCGGGCCGCCGCCGTGCTGGCGCGTGCCCGCCTGCAGGCGGCCCGGCTGCAGCCTCTCGTCGAGGCCG
ACGCGATCAGCCGCCAAGCCTATGACGACGCGGTGTCCCAGCGTGAGCAGGCCGCGGCGGACGTGGCGCAGGCGCGGGCC
ATCCTGGCACGCCGCCAGCTGGACCTGAAGTTCGCCACCGTGGAGGCGCCGATTTCGGGCCGCGTCGACCAGGCATTGGT
CAGCGAAGGGGCGCTGGTGAGCAGCGGCGACAGCAATCCGATGGCGCGCATTCAGCAGATCGATCAGGTGTATGTGGACG
TGCGTCGTCCCGCTTCCTCGCTCGACGCACTGCGCGAAGCCCTGGCCGCACCCGGCGCGAGCGCCGGGCACGGCTTGCCG
GTGACCTTGCTGCGAAGCGATGGGAAATCCTATGGCCTCAAGGCACGCATCCTGTTCTCGGGCATCAATGTCGATGCCGG
CACCGGCGAGGTCCTGCTGCGCGTGCTGGTGGACAACCCGCAGCGGCAGCTGCTGCCGGGCATGTTCGTGCGCGCGCGCG
TGCCCGGCGCCAGCTACGACGCCGCGCTGACGGTGCCGCAGCAGGCCGTGGTGCGGGTGAGCGGGCAGCCGCGGGTCTGG
GTTCTCGATGCGAGCCACCGCGCCAAGCTCGTCGCCGTGGCGTTGGGCGAGCGGGCGGGCAGGCGCTATCGCGTCCGCGC
CGGGTTGCACGCCGGGCAGCGGATCGTGGTCGAGGGCATGGAGCGGCTCTCCGACGGCACCAAGGTGAGTTCGCATGAGT
GGAAGTCGCCCGATTCCACCCCTGCGGCCTCCGCTAGCTGA

Upstream 100 bases:

>100_bases
CCATCGAATCTCCATCATAGCTCGGACGAAACACCATCGTCCAGATTCAGACTCCCGACCGTCTCGCCGGCCATCGACGG
CCTGTTTCCGAAGGAGTTCC

Downstream 100 bases:

>100_bases
GCAACGAAACACGAATCGCCATGTCCAAATTCTTCATCCAGCGCCCGGTCTTCGCCTGGGTGGTCGCCCTGTTCATCGTT
CTGGCGGGCGTCATCGCCAT

Product: multidrug efflux system protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MKIESPCRRAGLSVLLTVMLTACGQSEPQESAPPAPQVSATTLVPARLELSDELPGRVAALRVAEIRPQVSGIVQRRLFE
QGSEVRAGQPLFQINPAPFRADADTAAAALQRAAAVLARARLQAARLQPLVEADAISRQAYDDAVSQREQAAADVAQARA
ILARRQLDLKFATVEAPISGRVDQALVSEGALVSSGDSNPMARIQQIDQVYVDVRRPASSLDALREALAAPGASAGHGLP
VTLLRSDGKSYGLKARILFSGINVDAGTGEVLLRVLVDNPQRQLLPGMFVRARVPGASYDAALTVPQQAVVRVSGQPRVW
VLDASHRAKLVAVALGERAGRRYRVRAGLHAGQRIVVEGMERLSDGTKVSSHEWKSPDSTPAASAS

Sequences:

>Translated_386_residues
MKIESPCRRAGLSVLLTVMLTACGQSEPQESAPPAPQVSATTLVPARLELSDELPGRVAALRVAEIRPQVSGIVQRRLFE
QGSEVRAGQPLFQINPAPFRADADTAAAALQRAAAVLARARLQAARLQPLVEADAISRQAYDDAVSQREQAAADVAQARA
ILARRQLDLKFATVEAPISGRVDQALVSEGALVSSGDSNPMARIQQIDQVYVDVRRPASSLDALREALAAPGASAGHGLP
VTLLRSDGKSYGLKARILFSGINVDAGTGEVLLRVLVDNPQRQLLPGMFVRARVPGASYDAALTVPQQAVVRVSGQPRVW
VLDASHRAKLVAVALGERAGRRYRVRAGLHAGQRIVVEGMERLSDGTKVSSHEWKSPDSTPAASAS
>Mature_386_residues
MKIESPCRRAGLSVLLTVMLTACGQSEPQESAPPAPQVSATTLVPARLELSDELPGRVAALRVAEIRPQVSGIVQRRLFE
QGSEVRAGQPLFQINPAPFRADADTAAAALQRAAAVLARARLQAARLQPLVEADAISRQAYDDAVSQREQAAADVAQARA
ILARRQLDLKFATVEAPISGRVDQALVSEGALVSSGDSNPMARIQQIDQVYVDVRRPASSLDALREALAAPGASAGHGLP
VTLLRSDGKSYGLKARILFSGINVDAGTGEVLLRVLVDNPQRQLLPGMFVRARVPGASYDAALTVPQQAVVRVSGQPRVW
VLDASHRAKLVAVALGERAGRRYRVRAGLHAGQRIVVEGMERLSDGTKVSSHEWKSPDSTPAASAS

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=370, Percent_Identity=36.4864864864865, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1786668, Length=369, Percent_Identity=36.5853658536585, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1789929, Length=367, Percent_Identity=34.8773841961853, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI87082036, Length=333, Percent_Identity=29.1291291291291, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40984; Mature: 40984

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIESPCRRAGLSVLLTVMLTACGQSEPQESAPPAPQVSATTLVPARLELSDELPGRVAA
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECEEEECCCCCCCHHHH
LRVAEIRPQVSGIVQRRLFEQGSEVRAGQPLFQINPAPFRADADTAAAALQRAAAVLARA
HHHHHHCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
RLQAARLQPLVEADAISRQAYDDAVSQREQAAADVAQARAILARRQLDLKFATVEAPISG
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCC
RVDQALVSEGALVSSGDSNPMARIQQIDQVYVDVRRPASSLDALREALAAPGASAGHGLP
CHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCE
VTLLRSDGKSYGLKARILFSGINVDAGTGEVLLRVLVDNPQRQLLPGMFVRARVPGASYD
EEEEECCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCHHHHCCCCEEEEECCCCCCC
AALTVPQQAVVRVSGQPRVWVLDASHRAKLVAVALGERAGRRYRVRAGLHAGQRIVVEGM
CEEECCHHHEEEECCCCEEEEEECCCCCEEEEEEHHHHCCCEEEEEECCCCCCHHHHHHH
ERLSDGTKVSSHEWKSPDSTPAASAS
HHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIESPCRRAGLSVLLTVMLTACGQSEPQESAPPAPQVSATTLVPARLELSDELPGRVAA
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECEEEECCCCCCCHHHH
LRVAEIRPQVSGIVQRRLFEQGSEVRAGQPLFQINPAPFRADADTAAAALQRAAAVLARA
HHHHHHCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
RLQAARLQPLVEADAISRQAYDDAVSQREQAAADVAQARAILARRQLDLKFATVEAPISG
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCC
RVDQALVSEGALVSSGDSNPMARIQQIDQVYVDVRRPASSLDALREALAAPGASAGHGLP
CHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCE
VTLLRSDGKSYGLKARILFSGINVDAGTGEVLLRVLVDNPQRQLLPGMFVRARVPGASYD
EEEEECCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCHHHHCCCCEEEEECCCCCCC
AALTVPQQAVVRVSGQPRVWVLDASHRAKLVAVALGERAGRRYRVRAGLHAGQRIVVEGM
CEEECCHHHEEEECCCCEEEEEECCCCCEEEEEEHHHHCCCEEEEEECCCCCCHHHHHHH
ERLSDGTKVSSHEWKSPDSTPAASAS
HHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]