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The map label for this gene is leuA [H]

Identifier: 238024423

GI number: 238024423

Start: 1299349

End: 1301061

Strand: Direct

Name: leuA [H]

Synonym: bglu_2g10170

Alternate gene names: 238024423

Gene position: 1299349-1301061 (Clockwise)

Preceding gene: 238024422

Following gene: 238024426

Centisome position: 45.96

GC content: 69.06

Gene sequence:

>1713_bases
ATGATGCTCAAGAACCCCGCCGCCAAGTACCGCCCGTTCACGCCCGTCAACCTGACCGATCGCCAGTGGCCGTCGCGCAC
CATCACGCGCGCGCCGATCTGGATGAGCACCGACCTGCGCGACGGCAACCAGGCGCTGTTCGAGCCGATGGACGCGGCGC
GCAAGATGCGCATGTTCAAGATGCTGGTGGCGATCGGTTTCAAGGAAATCGAGGTGGCGTTTCCGTCGGCCTCCGAGACC
GACTTCAATTTCGTGCGCGAGCTGATCGAGGGCGGCCACATTCCCGACGACGTGACGATCGAAGTGCTGACGCAGGCGCG
CGACGACCTGATCGAGCGCACCTTCGCGTCGCTGCGCGGCGCCAAGCGCGCGATCGTCCACCTCTACAACGCGACCGCGC
CCGAATTCCGCCGCATCGTGTTCGGCCTCGAGCGCGAGGGCGTGAAGCAGCTGGCGGTCAGCGCGGCGCGCACCATCAAG
CGGCTGGCCGACGCGGCCGCCGACACGCAGTTCACGCTGCAATACAGCCCGGAAACCTTCACCGCCACCGAGCTCGACTT
CGCCAAGGAAGTCTGCGATGCCGTGTTCGACGTCTGGCAGCCGACGCCCGAGCGCAAGTCGATCGTGAACCTGCCGGCCA
CCGTCGAGGTGGGTACGCCGAACTACTACGCCGACCAGATCGAGTGGATGCACCGCAATCTCGCGCGGCGCGATTCGCTG
ATCCTCTCGGTGCATCCGCACAACGACCGCGGCACCGCGGTGGCGGCGGCCGAGCTGGCGGTGATGGCCGGCGCCGATCG
CATCGAGGGCTGCCTGTTCGGCAACGGCGAGCGCACCGGCAACGTCGATCTGGTCACGCTCGCGCTGAACCTCTACACGC
AGGGCGTCGATCCGGGCCTCGATTTCTCGCAGATCAACAGCGTCGCGCGCACCGCCGAGGAATGCACGCAGCTGCCCGTG
CATCCGCGTCATCCCTACGTCGGCGATCTGGTGTTCACGGCGTTCTCCGGCTCGCACCAGGACGCGATCAAGAAGGGCCT
CGCGGCGCAGCGCGCCGACGCGCGCTGGGAGGTGCCGTACCTGCCGATCGACCCGAGCGATCTGGGCCGCACCTACGATT
CGATCATTCGCGTCAACAGCCAGTCGGGCAAGGGCGGCATCGCCTATCTGCTCGAACAGGGCTATGGCGTGGCGCTGCCG
CGCCGCCTGCAGGTGGATTTCAGCGCGGCCGTGCAGCGCCTGACCGACCAGAGCGGGCAGGAGGTCTCGGCCGCGCAGAT
CTGGGCGCTGTTCGAATCCGAGTACGTCGCCAACCATGCGCCGCTGCGCTACCTCGGCCACGAGCTGAGCGAGCGCGGCG
GCCGCGAGACGATCGTGCTGAGCGCCGAGATCCACGGCGAGCGGCGCGAATTGCGCGGCGAGGGCAACGGCCCGCTCGAC
GCGCTGATGAACGCGCTGCGCACGCCGCTGCGCATCCAGCACTACGAGGAGCGGGCGCTGTCGCAGGGCGCCGACGCGAA
GGCGGTGGCGATCGCCGAGCTGGCAGGCGAGGGCGTGCCGGGCAGCGCGTTCGGTGTCGGCATCGACGCGAACCTGACCA
CCGCGTCGATCCGCGCCGTGATCAGCGGCATCAACCGCGCCTACGCGCGTGCCGGCCAGGCCGCGCAGGCCGGCTTCTTC
GCCGTGCAGCCGGCCGCGAGCGAGGTGGCCTGA

Upstream 100 bases:

>100_bases
TTTGCCGTTGTGCCGTCTCACCGCTGCGCGCGTGATCGCGATGCCGATCCGGATTGCCGATCCGCGCTGCCGCTTATGAA
AACCTGACCGAGAACCGATC

Downstream 100 bases:

>100_bases
GCGCGCCGCCGGCGTGCCCGATGCGGCGCGCGATGACGGCCGCCGCCTCGCCGGCCGCCGTCGAACGGCAGGTCACGGCG
AGGCTAATTTCGCGCGGTAT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MMLKNPAAKYRPFTPVNLTDRQWPSRTITRAPIWMSTDLRDGNQALFEPMDAARKMRMFKMLVAIGFKEIEVAFPSASET
DFNFVRELIEGGHIPDDVTIEVLTQARDDLIERTFASLRGAKRAIVHLYNATAPEFRRIVFGLEREGVKQLAVSAARTIK
RLADAAADTQFTLQYSPETFTATELDFAKEVCDAVFDVWQPTPERKSIVNLPATVEVGTPNYYADQIEWMHRNLARRDSL
ILSVHPHNDRGTAVAAAELAVMAGADRIEGCLFGNGERTGNVDLVTLALNLYTQGVDPGLDFSQINSVARTAEECTQLPV
HPRHPYVGDLVFTAFSGSHQDAIKKGLAAQRADARWEVPYLPIDPSDLGRTYDSIIRVNSQSGKGGIAYLLEQGYGVALP
RRLQVDFSAAVQRLTDQSGQEVSAAQIWALFESEYVANHAPLRYLGHELSERGGRETIVLSAEIHGERRELRGEGNGPLD
ALMNALRTPLRIQHYEERALSQGADAKAVAIAELAGEGVPGSAFGVGIDANLTTASIRAVISGINRAYARAGQAAQAGFF
AVQPAASEVA

Sequences:

>Translated_570_residues
MMLKNPAAKYRPFTPVNLTDRQWPSRTITRAPIWMSTDLRDGNQALFEPMDAARKMRMFKMLVAIGFKEIEVAFPSASET
DFNFVRELIEGGHIPDDVTIEVLTQARDDLIERTFASLRGAKRAIVHLYNATAPEFRRIVFGLEREGVKQLAVSAARTIK
RLADAAADTQFTLQYSPETFTATELDFAKEVCDAVFDVWQPTPERKSIVNLPATVEVGTPNYYADQIEWMHRNLARRDSL
ILSVHPHNDRGTAVAAAELAVMAGADRIEGCLFGNGERTGNVDLVTLALNLYTQGVDPGLDFSQINSVARTAEECTQLPV
HPRHPYVGDLVFTAFSGSHQDAIKKGLAAQRADARWEVPYLPIDPSDLGRTYDSIIRVNSQSGKGGIAYLLEQGYGVALP
RRLQVDFSAAVQRLTDQSGQEVSAAQIWALFESEYVANHAPLRYLGHELSERGGRETIVLSAEIHGERRELRGEGNGPLD
ALMNALRTPLRIQHYEERALSQGADAKAVAIAELAGEGVPGSAFGVGIDANLTTASIRAVISGINRAYARAGQAAQAGFF
AVQPAASEVA
>Mature_570_residues
MMLKNPAAKYRPFTPVNLTDRQWPSRTITRAPIWMSTDLRDGNQALFEPMDAARKMRMFKMLVAIGFKEIEVAFPSASET
DFNFVRELIEGGHIPDDVTIEVLTQARDDLIERTFASLRGAKRAIVHLYNATAPEFRRIVFGLEREGVKQLAVSAARTIK
RLADAAADTQFTLQYSPETFTATELDFAKEVCDAVFDVWQPTPERKSIVNLPATVEVGTPNYYADQIEWMHRNLARRDSL
ILSVHPHNDRGTAVAAAELAVMAGADRIEGCLFGNGERTGNVDLVTLALNLYTQGVDPGLDFSQINSVARTAEECTQLPV
HPRHPYVGDLVFTAFSGSHQDAIKKGLAAQRADARWEVPYLPIDPSDLGRTYDSIIRVNSQSGKGGIAYLLEQGYGVALP
RRLQVDFSAAVQRLTDQSGQEVSAAQIWALFESEYVANHAPLRYLGHELSERGGRETIVLSAEIHGERRELRGEGNGPLD
ALMNALRTPLRIQHYEERALSQGADAKAVAIAELAGEGVPGSAFGVGIDANLTTASIRAVISGINRAYARAGQAAQAGFF
AVQPAASEVA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=534, Percent_Identity=24.1573033707865, Blast_Score=109, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6324682, Length=565, Percent_Identity=46.9026548672566, Blast_Score=537, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6324225, Length=565, Percent_Identity=46.3716814159292, Blast_Score=529, Evalue=1e-151,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 62402; Mature: 62402

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLKNPAAKYRPFTPVNLTDRQWPSRTITRAPIWMSTDLRDGNQALFEPMDAARKMRMFK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCEEEECCCCCCCHHHHHHHHHHHHHHHHH
MLVAIGFKEIEVAFPSASETDFNFVRELIEGGHIPDDVTIEVLTQARDDLIERTFASLRG
HHHHHCCHHEEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AKRAIVHLYNATAPEFRRIVFGLEREGVKQLAVSAARTIKRLADAAADTQFTLQYSPETF
HHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
TATELDFAKEVCDAVFDVWQPTPERKSIVNLPATVEVGTPNYYADQIEWMHRNLARRDSL
CHHHHHHHHHHHHHHHHHHCCCCCCHHEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCE
ILSVHPHNDRGTAVAAAELAVMAGADRIEGCLFGNGERTGNVDLVTLALNLYTQGVDPGL
EEEEECCCCCCCHHHHHHHHHHCCCHHHCEEEECCCCCCCCEEEEEEEEEHHHCCCCCCC
DFSQINSVARTAEECTQLPVHPRHPYVGDLVFTAFSGSHQDAIKKGLAAQRADARWEVPY
CHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHEEECCCCHHHHHHHHHHHHCCCCCCCCCC
LPIDPSDLGRTYDSIIRVNSQSGKGGIAYLLEQGYGVALPRRLQVDFSAAVQRLTDQSGQ
CCCCHHHHHHHHHHHEEECCCCCCCCHHHEECCCCCCCCCCEEEECHHHHHHHHHCCCCC
EVSAAQIWALFESEYVANHAPLRYLGHELSERGGRETIVLSAEIHGERRELRGEGNGPLD
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEEEEECCCHHHHCCCCCCCHH
ALMNALRTPLRIQHYEERALSQGADAKAVAIAELAGEGVPGSAFGVGIDANLTTASIRAV
HHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHH
ISGINRAYARAGQAAQAGFFAVQPAASEVA
HHHHHHHHHHCCCHHHCCEEEECCCHHCCC
>Mature Secondary Structure
MMLKNPAAKYRPFTPVNLTDRQWPSRTITRAPIWMSTDLRDGNQALFEPMDAARKMRMFK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCEEEECCCCCCCHHHHHHHHHHHHHHHHH
MLVAIGFKEIEVAFPSASETDFNFVRELIEGGHIPDDVTIEVLTQARDDLIERTFASLRG
HHHHHCCHHEEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AKRAIVHLYNATAPEFRRIVFGLEREGVKQLAVSAARTIKRLADAAADTQFTLQYSPETF
HHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
TATELDFAKEVCDAVFDVWQPTPERKSIVNLPATVEVGTPNYYADQIEWMHRNLARRDSL
CHHHHHHHHHHHHHHHHHHCCCCCCHHEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCE
ILSVHPHNDRGTAVAAAELAVMAGADRIEGCLFGNGERTGNVDLVTLALNLYTQGVDPGL
EEEEECCCCCCCHHHHHHHHHHCCCHHHCEEEECCCCCCCCEEEEEEEEEHHHCCCCCCC
DFSQINSVARTAEECTQLPVHPRHPYVGDLVFTAFSGSHQDAIKKGLAAQRADARWEVPY
CHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHEEECCCCHHHHHHHHHHHHCCCCCCCCCC
LPIDPSDLGRTYDSIIRVNSQSGKGGIAYLLEQGYGVALPRRLQVDFSAAVQRLTDQSGQ
CCCCHHHHHHHHHHHEEECCCCCCCCHHHEECCCCCCCCCCEEEECHHHHHHHHHCCCCC
EVSAAQIWALFESEYVANHAPLRYLGHELSERGGRETIVLSAEIHGERRELRGEGNGPLD
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEEEEECCCHHHHCCCCCCCHH
ALMNALRTPLRIQHYEERALSQGADAKAVAIAELAGEGVPGSAFGVGIDANLTTASIRAV
HHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHH
ISGINRAYARAGQAAQAGFFAVQPAASEVA
HHHHHHHHHHCCCHHHCCEEEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA