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The map label for this gene is leuB [H]

Identifier: 238024254

GI number: 238024254

Start: 1013957

End: 1015024

Strand: Direct

Name: leuB [H]

Synonym: bglu_2g08360

Alternate gene names: 238024254

Gene position: 1013957-1015024 (Clockwise)

Preceding gene: 238024253

Following gene: 238024255

Centisome position: 35.86

GC content: 68.07

Gene sequence:

>1068_bases
ATGAAGATTGCAGTGCTGCCGGGCGACGGCATCGGCCCCGAGATCGTCAAGGAAGCGGTGAAGGTGCTCAACGCGCTCGA
CGAGACCTTCGAACTGGAGCAGGCGCCGGTGGGCGGCGCCGGCTACGAGGCGGCCGGCCATCCGCTGCCGGAGGCGACGC
TGGCGCTCGCGAAGCAGGCGGACGCGATCCTGTTCGGCGCGGTCGGCGACTGGAAGTACGATACGCTCGAACGCGCGCTG
CGTCCGGAGCAGGCGATCCTCGGCCTGCGCAAGCATCTGGAGCTGTTCGCGAACTTCCGCCCGGCGATCTGCTACGCGCA
GCTCGTCGACGCCTCGCCGCTCAAGCCTCAACTGATTGCCGGGCTCGACATCCTGATCGTGCGGGAGCTGAACGGCGACA
TCTACTTCGGCCAGCCGCGCGGCACGCGCGAGGCGCCGGACGGCCCGTTCGCCGGGGCCCGCGAAGGCTTCGACACGATG
CGCTACTCGGAGCCCGAAGTGCGCCGCATCGCGCACGTGGCGTTCCAGGCGGCGCAAAAGCGCGGCAGGAAGCTGCTGTC
GGTCGACAAGGCCAACGTGCTCGAAACCTCGCAGTTCTGGCGCGACGTGATGATCGACGTGTCCAAGCAATACGCCGACG
TCGAGCTTTCGCACATGTACGTCGACAACGCGGCGATGCAGCTCGCCAAGGCGCCGAAGCAGTTCGACGTGATCGTCACC
GGCAACATGTTCGGCGACATCCTTTCCGACGAGGCCGCGATGCTGACCGGCTCGATCGGCATGCTGCCGTCGGCCTCGCT
CGACAAGAACAACAAGGGCCTGTACGAGCCCTCGCACGGCTCGGCACCGGACATCGCGGGCAAGGGGGTGGCCAATCCGC
TCGCCACCATCCTGTCGGCCGCGATGCTGCTGCGCTATTCGCTGAATCGCGCCGAGCAGGCCGAGCGCATCGAGCGCGCG
GTGCAGAAGGTGCTCGAACAGGGCTACCGCACCGGCGACATCGCCACGCCGGGCTGCCGGCAGGTCGGCACCGAGCAGAT
GGGCGACGCGGTGGTCGCCGCGCTGTAA

Upstream 100 bases:

>100_bases
GCCTGATCGGCTGACCGATGGCCGCGGCGCCGCGCGCGAGGCCGGCCCCGGCCGCCTGCGCGGGCGCGCCCGAATTCCCA
ACCTACCGAGGAAAAATCGC

Downstream 100 bases:

>100_bases
CGCCGCCCCGGCGCGCCTGGCGGCCCGCAAAGGAGGGCTGCGGCGCGCCGGTCGCGATTGTTTCATGGGGCCGTTGTCCG
GCCCTGCAGGTTTGTGTAGA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKIAVLPGDGIGPEIVKEAVKVLNALDETFELEQAPVGGAGYEAAGHPLPEATLALAKQADAILFGAVGDWKYDTLERAL
RPEQAILGLRKHLELFANFRPAICYAQLVDASPLKPQLIAGLDILIVRELNGDIYFGQPRGTREAPDGPFAGAREGFDTM
RYSEPEVRRIAHVAFQAAQKRGRKLLSVDKANVLETSQFWRDVMIDVSKQYADVELSHMYVDNAAMQLAKAPKQFDVIVT
GNMFGDILSDEAAMLTGSIGMLPSASLDKNNKGLYEPSHGSAPDIAGKGVANPLATILSAAMLLRYSLNRAEQAERIERA
VQKVLEQGYRTGDIATPGCRQVGTEQMGDAVVAAL

Sequences:

>Translated_355_residues
MKIAVLPGDGIGPEIVKEAVKVLNALDETFELEQAPVGGAGYEAAGHPLPEATLALAKQADAILFGAVGDWKYDTLERAL
RPEQAILGLRKHLELFANFRPAICYAQLVDASPLKPQLIAGLDILIVRELNGDIYFGQPRGTREAPDGPFAGAREGFDTM
RYSEPEVRRIAHVAFQAAQKRGRKLLSVDKANVLETSQFWRDVMIDVSKQYADVELSHMYVDNAAMQLAKAPKQFDVIVT
GNMFGDILSDEAAMLTGSIGMLPSASLDKNNKGLYEPSHGSAPDIAGKGVANPLATILSAAMLLRYSLNRAEQAERIERA
VQKVLEQGYRTGDIATPGCRQVGTEQMGDAVVAAL
>Mature_355_residues
MKIAVLPGDGIGPEIVKEAVKVLNALDETFELEQAPVGGAGYEAAGHPLPEATLALAKQADAILFGAVGDWKYDTLERAL
RPEQAILGLRKHLELFANFRPAICYAQLVDASPLKPQLIAGLDILIVRELNGDIYFGQPRGTREAPDGPFAGAREGFDTM
RYSEPEVRRIAHVAFQAAQKRGRKLLSVDKANVLETSQFWRDVMIDVSKQYADVELSHMYVDNAAMQLAKAPKQFDVIVT
GNMFGDILSDEAAMLTGSIGMLPSASLDKNNKGLYEPSHGSAPDIAGKGVANPLATILSAAMLLRYSLNRAEQAERIERA
VQKVLEQGYRTGDIATPGCRQVGTEQMGDAVVAAL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=353, Percent_Identity=30.8781869688385, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI28178821, Length=310, Percent_Identity=27.741935483871, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI28178816, Length=310, Percent_Identity=27.741935483871, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI4758582, Length=343, Percent_Identity=27.4052478134111, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI28178838, Length=325, Percent_Identity=27.6923076923077, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI28178819, Length=141, Percent_Identity=34.0425531914894, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI87081683, Length=355, Percent_Identity=53.8028169014084, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1788101, Length=365, Percent_Identity=33.4246575342466, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1787381, Length=398, Percent_Identity=24.3718592964824, Blast_Score=78, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71986051, Length=340, Percent_Identity=30.2941176470588, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI25144293, Length=337, Percent_Identity=31.1572700296736, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17550882, Length=340, Percent_Identity=29.7058823529412, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17505779, Length=356, Percent_Identity=29.2134831460674, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=365, Percent_Identity=46.027397260274, Blast_Score=285, Evalue=6e-78,
Organism=Saccharomyces cerevisiae, GI6322097, Length=371, Percent_Identity=36.1185983827493, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6324291, Length=336, Percent_Identity=29.7619047619048, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324709, Length=358, Percent_Identity=29.3296089385475, Blast_Score=112, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24643270, Length=341, Percent_Identity=31.9648093841642, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24643268, Length=341, Percent_Identity=31.9648093841642, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24661184, Length=344, Percent_Identity=29.3604651162791, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI281362242, Length=343, Percent_Identity=29.7376093294461, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648872, Length=343, Percent_Identity=29.7376093294461, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI161078635, Length=349, Percent_Identity=26.3610315186246, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI161078637, Length=349, Percent_Identity=26.3610315186246, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI161078633, Length=349, Percent_Identity=26.3610315186246, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24650122, Length=349, Percent_Identity=26.3610315186246, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI161078639, Length=364, Percent_Identity=26.3736263736264, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI20130355, Length=172, Percent_Identity=30.8139534883721, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38343; Mature: 38343

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVLPGDGIGPEIVKEAVKVLNALDETFELEQAPVGGAGYEAAGHPLPEATLALAKQA
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHC
DAILFGAVGDWKYDTLERALRPEQAILGLRKHLELFANFRPAICYAQLVDASPLKPQLIA
CEEEEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHC
GLDILIVRELNGDIYFGQPRGTREAPDGPFAGAREGFDTMRYSEPEVRRIAHVAFQAAQK
CCEEEEEEECCCCEEECCCCCCCCCCCCCCCCCHHCHHHHCCCCHHHHHHHHHHHHHHHH
RGRKLLSVDKANVLETSQFWRDVMIDVSKQYADVELSHMYVDNAAMQLAKAPKQFDVIVT
HCCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHCCCCEEEEEE
GNMFGDILSDEAAMLTGSIGMLPSASLDKNNKGLYEPSHGSAPDIAGKGVANPLATILSA
CCHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AMLLRYSLNRAEQAERIERAVQKVLEQGYRTGDIATPGCRQVGTEQMGDAVVAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCHHHHCCHHHHCC
>Mature Secondary Structure
MKIAVLPGDGIGPEIVKEAVKVLNALDETFELEQAPVGGAGYEAAGHPLPEATLALAKQA
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHC
DAILFGAVGDWKYDTLERALRPEQAILGLRKHLELFANFRPAICYAQLVDASPLKPQLIA
CEEEEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHC
GLDILIVRELNGDIYFGQPRGTREAPDGPFAGAREGFDTMRYSEPEVRRIAHVAFQAAQK
CCEEEEEEECCCCEEECCCCCCCCCCCCCCCCCHHCHHHHCCCCHHHHHHHHHHHHHHHH
RGRKLLSVDKANVLETSQFWRDVMIDVSKQYADVELSHMYVDNAAMQLAKAPKQFDVIVT
HCCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHCCCCEEEEEE
GNMFGDILSDEAAMLTGSIGMLPSASLDKNNKGLYEPSHGSAPDIAGKGVANPLATILSA
CCHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AMLLRYSLNRAEQAERIERAVQKVLEQGYRTGDIATPGCRQVGTEQMGDAVVAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12754231 [H]