Click here to switch to the map view.

The map label for this gene is leuD [H]

Identifier: 238024253

GI number: 238024253

Start: 1013220

End: 1013870

Strand: Direct

Name: leuD [H]

Synonym: bglu_2g08350

Alternate gene names: 238024253

Gene position: 1013220-1013870 (Clockwise)

Preceding gene: 238024252

Following gene: 238024254

Centisome position: 35.84

GC content: 64.67

Gene sequence:

>651_bases
ATGGAAAAATTCACTGTACACACCGGCGTCGTGGCGCCGCTCGATCGCGCCAACGTGGACACGGACGCGATCATCCCGAA
GCAATTCCTGAAGTCGATCAAGCGCACCGGCTTCGGCCCGAACGCGTTCGACGAATGGCGCTACCTCGATCACGGCGAGC
CGGGCCAGGACAACTCGAAGCGGCCGCTGAACCCCGATTTCGTGCTGAACCAGCCGCGTTACCAGGGCGCCTCGGTGCTG
CTCGCGCGCGAGAACTTCGGCTGCGGCAGCTCGCGCGAGCATGCGCCGTGGGCGCTGCAGCAGTACGGCTTCCGCGCGAT
CATCGCGCCGAGCTTCGCCGACATCTTCTTCAACAACTGCTTCAAGAACGGCCTGCTGCCGATCGTGCTGGGCGAGCGCG
AGGTCGCGCGCCTGTTCGACGAGACCTATGCGTTCAACGGCTACCAGTTGACGATCGATCTCGACGCGCAGGTGGTGCGC
ACGGGCGACAATCGCGAGTACCCGTTCGAGGTCAGCGCGTTTCGCAAGTACTGCCTGCTGAACGGCTTCGACGACATCGG
CCTGACGCTGCGCCACGCCGACAAGATCCGCCAGTTCGAGGCCGAGCGGATCGCGCGCCAGCCCTGGTTGAACAACCGCC
TGATCGGCTGA

Upstream 100 bases:

>100_bases
CGCGCGATCACGCGCGCGGCGGACTAGGCGCGGCGCGACCCGCCCGACGGACAAACGATTTCGCCGGCCCGCGGGCCGGC
ATCCAATCTGGTCACGGATC

Downstream 100 bases:

>100_bases
CCGATGGCCGCGGCGCCGCGCGCGAGGCCGGCCCCGGCCGCCTGCGCGGGCGCGCCCGAATTCCCAACCTACCGAGGAAA
AATCGCATGAAGATTGCAGT

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MEKFTVHTGVVAPLDRANVDTDAIIPKQFLKSIKRTGFGPNAFDEWRYLDHGEPGQDNSKRPLNPDFVLNQPRYQGASVL
LARENFGCGSSREHAPWALQQYGFRAIIAPSFADIFFNNCFKNGLLPIVLGEREVARLFDETYAFNGYQLTIDLDAQVVR
TGDNREYPFEVSAFRKYCLLNGFDDIGLTLRHADKIRQFEAERIARQPWLNNRLIG

Sequences:

>Translated_216_residues
MEKFTVHTGVVAPLDRANVDTDAIIPKQFLKSIKRTGFGPNAFDEWRYLDHGEPGQDNSKRPLNPDFVLNQPRYQGASVL
LARENFGCGSSREHAPWALQQYGFRAIIAPSFADIFFNNCFKNGLLPIVLGEREVARLFDETYAFNGYQLTIDLDAQVVR
TGDNREYPFEVSAFRKYCLLNGFDDIGLTLRHADKIRQFEAERIARQPWLNNRLIG
>Mature_216_residues
MEKFTVHTGVVAPLDRANVDTDAIIPKQFLKSIKRTGFGPNAFDEWRYLDHGEPGQDNSKRPLNPDFVLNQPRYQGASVL
LARENFGCGSSREHAPWALQQYGFRAIIAPSFADIFFNNCFKNGLLPIVLGEREVARLFDETYAFNGYQLTIDLDAQVVR
TGDNREYPFEVSAFRKYCLLNGFDDIGLTLRHADKIRQFEAERIARQPWLNNRLIG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=204, Percent_Identity=59.8039215686275, Blast_Score=244, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6321429, Length=214, Percent_Identity=47.6635514018692, Blast_Score=188, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 24689; Mature: 24689

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFTVHTGVVAPLDRANVDTDAIIPKQFLKSIKRTGFGPNAFDEWRYLDHGEPGQDNSK
CCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCC
RPLNPDFVLNQPRYQGASVLLARENFGCGSSREHAPWALQQYGFRAIIAPSFADIFFNNC
CCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCEEEECCHHHHHHHHHH
FKNGLLPIVLGEREVARLFDETYAFNGYQLTIDLDAQVVRTGDNREYPFEVSAFRKYCLL
HHCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCEEEEECCCCCCCCEEHHHHHHHHHH
NGFDDIGLTLRHADKIRQFEAERIARQPWLNNRLIG
CCCCHHCEEHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MEKFTVHTGVVAPLDRANVDTDAIIPKQFLKSIKRTGFGPNAFDEWRYLDHGEPGQDNSK
CCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCC
RPLNPDFVLNQPRYQGASVLLARENFGCGSSREHAPWALQQYGFRAIIAPSFADIFFNNC
CCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCEEEECCHHHHHHHHHH
FKNGLLPIVLGEREVARLFDETYAFNGYQLTIDLDAQVVRTGDNREYPFEVSAFRKYCLL
HHCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCEEEEECCCCCCCCEEHHHHHHHHHH
NGFDDIGLTLRHADKIRQFEAERIARQPWLNNRLIG
CCCCHHCEEHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA