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The map label for this gene is hdhA [C]

Identifier: 238024199

GI number: 238024199

Start: 942749

End: 943651

Strand: Reverse

Name: hdhA [C]

Synonym: bglu_2g07790

Alternate gene names: 238024199

Gene position: 943651-942749 (Counterclockwise)

Preceding gene: 238024200

Following gene: 238024183

Centisome position: 33.38

GC content: 60.24

Gene sequence:

>903_bases
ATGTATGAAGTTCTTGATCAGCGGGGGCGCCGGGTCGTCATTACCGGCGCCAATAGCGGCACGGGCAGGGAGGCGGCCAG
GCGATTGGCGGCGGCTGGTGCCGACCTCGTGATGGCGGTCCGCAGCGAGTCGAAGGGCGATGCTGCGCGTCGGGATTTGC
AGGAGCAGTTTCCAGGCGCCTCGATTGAGGTTCGCATGATGGATCTCTCCAGCCTCGCGAGCGTGAGGGAGTTTGCCACC
CAGTTGCTCGAGGAAGGGCGTCCCCTCGACCTGCTCGTGAACAACGCCGGCGTCATGATGCCTCCGAGGCGCGTACTGAG
TTCCGACGGATTCGAGCTACAGTTTGCGACGAATTTCCTCGGTCACTTTGCACTGACCAATCTTCTGTTGCCGCTCTTGC
TCGAAGCGAAGTCGCCGCGCGTCACCACGATGACAAGCAGCGCCGCGATCGGAGCCAAAATCAATTTCGACGATCTTCAA
GGTGAAAGGTCGTACAAGCCCATGGCCGCGTATGCGCAGTCCAAGCTGGCCTGCCTGTTGCTGGCAAATCGATTGGCAGA
AATTGCACACGAACGTCGCTGGCCCTTGCTCAGTACGAGCGCCCATCCGGGCCATGCGCGCACCAATCTACAGACAAGCG
GCCCGAACCTGGGAACTGATTCAACCCGCAAGAGGCTATTGTTCAAGCTTGTACCGTCCATGAGCGTGGAGTGGGCCACT
GATTCGCTCTTGCAAGCGGCGCTGGAGCCGACAGCCACGCAGGGCGCATTCTACGGCCCACGATTTCTGCTCATCGGGAA
CTCACATATTATCGAACAACCTCACTCCGCCAAGCGCGCGGATTCCATTCGGCTGTGGCACGTAGCGGAAACATTGACTC
AAACGAGGCCGCCGCCGGTGTGA

Upstream 100 bases:

>100_bases
GGCTAGGCCGCCGCTGCCCCGGCAGTGCCACGCTATAGGTTCCAGGCAAGGGCTTGCGACGATACCTGGGCAGCCGAATC
AACTCATAGGAGAGGCCCGG

Downstream 100 bases:

>100_bases
TGGACAGAAGTACGGACTTGATCTGACAGCAAGGCCTTGCCAAGCGGTGCGGTTGCCCGTTTTGATAGAGGTGCGAAGCT
TCATCGAAGCAGCGCGCAAG

Product: Short-chain dehydrogenase/reductase SDR

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MYEVLDQRGRRVVITGANSGTGREAARRLAAAGADLVMAVRSESKGDAARRDLQEQFPGASIEVRMMDLSSLASVREFAT
QLLEEGRPLDLLVNNAGVMMPPRRVLSSDGFELQFATNFLGHFALTNLLLPLLLEAKSPRVTTMTSSAAIGAKINFDDLQ
GERSYKPMAAYAQSKLACLLLANRLAEIAHERRWPLLSTSAHPGHARTNLQTSGPNLGTDSTRKRLLFKLVPSMSVEWAT
DSLLQAALEPTATQGAFYGPRFLLIGNSHIIEQPHSAKRADSIRLWHVAETLTQTRPPPV

Sequences:

>Translated_300_residues
MYEVLDQRGRRVVITGANSGTGREAARRLAAAGADLVMAVRSESKGDAARRDLQEQFPGASIEVRMMDLSSLASVREFAT
QLLEEGRPLDLLVNNAGVMMPPRRVLSSDGFELQFATNFLGHFALTNLLLPLLLEAKSPRVTTMTSSAAIGAKINFDDLQ
GERSYKPMAAYAQSKLACLLLANRLAEIAHERRWPLLSTSAHPGHARTNLQTSGPNLGTDSTRKRLLFKLVPSMSVEWAT
DSLLQAALEPTATQGAFYGPRFLLIGNSHIIEQPHSAKRADSIRLWHVAETLTQTRPPPV
>Mature_300_residues
MYEVLDQRGRRVVITGANSGTGREAARRLAAAGADLVMAVRSESKGDAARRDLQEQFPGASIEVRMMDLSSLASVREFAT
QLLEEGRPLDLLVNNAGVMMPPRRVLSSDGFELQFATNFLGHFALTNLLLPLLLEAKSPRVTTMTSSAAIGAKINFDDLQ
GERSYKPMAAYAQSKLACLLLANRLAEIAHERRWPLLSTSAHPGHARTNLQTSGPNLGTDSTRKRLLFKLVPSMSVEWAT
DSLLQAALEPTATQGAFYGPRFLLIGNSHIIEQPHSAKRADSIRLWHVAETLTQTRPPPV

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI225579078, Length=206, Percent_Identity=41.747572815534, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI166795268, Length=205, Percent_Identity=40.4878048780488, Blast_Score=134, Evalue=8e-32,
Organism=Homo sapiens, GI186928839, Length=202, Percent_Identity=41.5841584158416, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI10190746, Length=218, Percent_Identity=41.743119266055, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI193804850, Length=205, Percent_Identity=38.0487804878049, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI19923931, Length=170, Percent_Identity=42.3529411764706, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI7706523, Length=306, Percent_Identity=31.0457516339869, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI146231950, Length=207, Percent_Identity=38.1642512077295, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI72534732, Length=153, Percent_Identity=36.6013071895425, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI13375997, Length=152, Percent_Identity=36.1842105263158, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI20149619, Length=216, Percent_Identity=28.7037037037037, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17558006, Length=211, Percent_Identity=34.1232227488152, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17532805, Length=217, Percent_Identity=34.5622119815668, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI193207620, Length=221, Percent_Identity=34.3891402714932, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71992402, Length=222, Percent_Identity=31.5315315315315, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17505416, Length=195, Percent_Identity=32.3076923076923, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17532791, Length=214, Percent_Identity=28.9719626168224, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32565532, Length=138, Percent_Identity=35.5072463768116, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI72001364, Length=214, Percent_Identity=33.6448598130841, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17535059, Length=212, Percent_Identity=30.188679245283, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI115533174, Length=175, Percent_Identity=32.5714285714286, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115533172, Length=175, Percent_Identity=32.5714285714286, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324820, Length=273, Percent_Identity=30.4029304029304, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324148, Length=201, Percent_Identity=32.3383084577114, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323882, Length=174, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45554248, Length=256, Percent_Identity=38.28125, Blast_Score=140, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24640117, Length=256, Percent_Identity=38.28125, Blast_Score=140, Evalue=8e-34,
Organism=Drosophila melanogaster, GI28573296, Length=249, Percent_Identity=36.5461847389558, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19921754, Length=205, Percent_Identity=38.5365853658537, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI22024069, Length=182, Percent_Identity=40.6593406593407, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24586328, Length=182, Percent_Identity=40.1098901098901, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161076371, Length=182, Percent_Identity=40.1098901098901, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24656154, Length=263, Percent_Identity=33.4600760456274, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI19922628, Length=263, Percent_Identity=33.4600760456274, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI45553409, Length=180, Percent_Identity=35, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24647948, Length=180, Percent_Identity=35, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24647946, Length=179, Percent_Identity=35.195530726257, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI20129347, Length=299, Percent_Identity=32.7759197324415, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24647931, Length=193, Percent_Identity=40.9326424870466, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21358495, Length=248, Percent_Identity=27.8225806451613, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 32807; Mature: 32807

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEVLDQRGRRVVITGANSGTGREAARRLAAAGADLVMAVRSESKGDAARRDLQEQFPGA
CCCCHHHCCCEEEEECCCCCCCHHHHHHHHHCCCHHEEEECCCCCCHHHHHHHHHHCCCC
SIEVRMMDLSSLASVREFATQLLEEGRPLDLLVNNAGVMMPPRRVLSSDGFELQFATNFL
CEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHCCCCCEEEHHHHHH
GHFALTNLLLPLLLEAKSPRVTTMTSSAAIGAKINFDDLQGERSYKPMAAYAQSKLACLL
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEECHHHCCCCCCCCHHHHHHHHHHHHHH
LANRLAEIAHERRWPLLSTSAHPGHARTNLQTSGPNLGTDSTRKRLLFKLVPSMSVEWAT
HHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHH
DSLLQAALEPTATQGAFYGPRFLLIGNSHIIEQPHSAKRADSIRLWHVAETLTQTRPPPV
HHHHHHHHCCCCCCCCCCCCEEEEECCCHHCCCCCCCCCCCCEEEHHHHHHHHCCCCCCC
>Mature Secondary Structure
MYEVLDQRGRRVVITGANSGTGREAARRLAAAGADLVMAVRSESKGDAARRDLQEQFPGA
CCCCHHHCCCEEEEECCCCCCCHHHHHHHHHCCCHHEEEECCCCCCHHHHHHHHHHCCCC
SIEVRMMDLSSLASVREFATQLLEEGRPLDLLVNNAGVMMPPRRVLSSDGFELQFATNFL
CEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHCCCCCEEEHHHHHH
GHFALTNLLLPLLLEAKSPRVTTMTSSAAIGAKINFDDLQGERSYKPMAAYAQSKLACLL
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEECHHHCCCCCCCCHHHHHHHHHHHHHH
LANRLAEIAHERRWPLLSTSAHPGHARTNLQTSGPNLGTDSTRKRLLFKLVPSMSVEWAT
HHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHH
DSLLQAALEPTATQGAFYGPRFLLIGNSHIIEQPHSAKRADSIRLWHVAETLTQTRPPPV
HHHHHHHHCCCCCCCCCCCCEEEEECCCHHCCCCCCCCCCCCEEEHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383668 [H]