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The map label for this gene is glgX [H]

Identifier: 238024143

GI number: 238024143

Start: 848310

End: 850565

Strand: Reverse

Name: glgX [H]

Synonym: bglu_2g07200

Alternate gene names: 238024143

Gene position: 850565-848310 (Counterclockwise)

Preceding gene: 238024144

Following gene: 238024137

Centisome position: 30.08

GC content: 67.69

Gene sequence:

>2256_bases
ATGTCCACCACGCCCTCCCTCCAGCAGCCCGGCCTGCGCGACGCCCTCGCCGCGCCCCCCGATTCCGCGGGCCGCTTCGG
CCGCGCCATCCAGGAAGGGCTGCCGTTTCCGCTTGGCGCGACATGGAACGGACACGGCGTCAATTTCGCGCTGTTCTCCG
CCCACGCCACCAAGGTCGAGCTCTGCCTGTTCGACGAAGACGGCAAGACCGAGCGGGAGCGCATCGCGCTGCCCGAATAC
ACCGACGAGGTTTTCCATGTGTTCGTGCCGGGCCTGGAGCCGGGCGCCGTGTACGGCTACCGCGTGCACGGGCCCTACGA
GCCCGAGCGCGGCCACCGCTTCAATCCCAACAAGCTGCTGCTCGACCCTTATGCAAAGGCCCATGTCGGCGAACTCGTCT
GGGCGCCCGAAATCTTCGGCTACACGCTCGACTCGCCCGACGGCGACCTGTCGTTCGACGAGCGCGACAGCGCGCCGTTC
GTGCCGAAATGCAAGGTGGTCGATTCGACGTTCTCCTGGACCCATCCGGACCGCCACGTCGTGGCGCCGGATCGCGTCAT
CGTCTACGAAGCGCATGTGCGCGGCTTCACGAAAATGCATCCGGACCTGCCCGAGCCGCTGCGCGGCACCTTCGCCGGCT
TTGCCCACCCACGCGTGATCGATTACATCCGCGGGCTCGGCGTGACGACGGTCGAACTGCTGCCGGTGCATACCTTCGTC
AACGACAGCTACCTGCTCGACAAGGGCCTGACGAACTACTGGGGCTACAACACGATCGGCTTCTTTGCCGCCGATCCGCG
CTATTTCGCGGACAAGAAGGCGTCCTTGGGCGAATTCAAGGAGATGGTGGACCGCTACCACCAGGCCGGGCTCGAAGTGA
TCCTCGACGTCGTGTACAACCACACGGCCGAAGGCAACGAGCTCGGCCCCACGCTGTCGTTCAAGGGTATCGACAACGCC
TCCTACTACCGGCTGATGCCCGACCAGCCTCGCTACTACATCAACGACACCGGCACCGGGAACACCTTCAACCTGTCGTG
CCCGCGCGTGCTGCAGATGGTCACCGACAGCCTGCGCTACTGGGTGGCGGAGATGAAGGTGGACGGCTTCCGGTTCGACC
TCGCCACCATCCTCGGCCGCGAGCCGCACGGCTTCGACGAGGGCGGCGGCTTCCTCGACGCGTGCCGCCAGGACCCCCTG
CTGGCCAGCGTAAGGCTGATCGCCGAGCCCTGGGACTGCGGGCCGGGCGGCTATCAGGTCGGCGGATTCCCGCCGGGCTG
GTCGGAGTGGAACGACACGTTCCGCGACACGGTGCGCGCGTACTGGAAGGGCGACGACGCCATGGCCGCCGAACTGGCCA
AGCGGATCACCGCGTCGGGCGACAAATTCAACCGTCGCGGCCGGCGCCCGTGGGCCAGCGTCAATTTCATCACGGCACAC
GACGGCTTCACGCTGAACGACCTGGTGTCGTACAACGACAAGCACAACGAGGCGAACGGCGAGGACAACCGGGACGGCCA
CGCCGACAACCGCTCCTGGAACATGGGGGTCGAAGGCCCGACCGACGATCCGGCGATCCGCGAGCAGCGCGAACGCCAGA
AGCGCAACCTGCTGGCGACGCTGCTGCTGTCGCAAGGCACGCCGATGATCCTCGGCGGCGACGAGTTCGGCCGCACCCAG
CGCGGCAACAACAACGCCTATTGCCAGGACAACGAAATCAGCTGGGTCGACTGGAACGGCATCGACGCGGACGGCCAGGC
GCTGGCCGAATTCGTGCGCAAGCTGACCACCCTGCGGCGGCGCCTGCCGGTGCTGCGGCGCGGGCGGTTCCTGACCGGCG
AGCACAACGAGGCGCTCGGCGTGACGGACGCGCGCTGGATATCGGCGGCGGGCGACGACCTCACCCAGGCGCAATGGGAC
GACCCGTCGATGCGCTGCTTCGGGCTCGTGATCGACGGGCGCGCCCAGGCGAGCGGCATCGTCAAGGCGGCCTCGGACGC
CACCTTGCTGCTCGTGTTCAACGCCCATTTCGACGTGGTGGACTTCACGCTGCCCGCGGTCCCGGACGGCGAGCACTGGA
CCTGCCTGCTCGACACCAACGTGCCGGTGCGCCAGGCGCTGCCCGAATTCCCGAGCGGCGCCGTGTACCAGGTGACGGGC
CGCTCGCTGCTGCTGTTCGCGCTCGAGGCGCCGAGCGCCGCGACGCAGCGCGTGTTCGACCGTCTCGAGACGGTATTGGT
CGACGGCGCCGACTGA

Upstream 100 bases:

>100_bases
CGCCCATCGCGGCGGTGCGCCGTCTGGCGCGGCGATCGCGCCGATATGGCGCGTGGCCCGGATCTTGCTCTCATCCCCTG
CTCCTTCCAGGAGCCTCGAC

Downstream 100 bases:

>100_bases
GGGCGCCGGCGCGCGCCGGGCGCGGCTCGGCGAGCGCCCCGGTGGAGCGCGGCAGGTAGATCCTCACGGTGGTGCCGTGG
CCCACCTCGCTATCGATGCG

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 751; Mature: 750

Protein sequence:

>751_residues
MSTTPSLQQPGLRDALAAPPDSAGRFGRAIQEGLPFPLGATWNGHGVNFALFSAHATKVELCLFDEDGKTERERIALPEY
TDEVFHVFVPGLEPGAVYGYRVHGPYEPERGHRFNPNKLLLDPYAKAHVGELVWAPEIFGYTLDSPDGDLSFDERDSAPF
VPKCKVVDSTFSWTHPDRHVVAPDRVIVYEAHVRGFTKMHPDLPEPLRGTFAGFAHPRVIDYIRGLGVTTVELLPVHTFV
NDSYLLDKGLTNYWGYNTIGFFAADPRYFADKKASLGEFKEMVDRYHQAGLEVILDVVYNHTAEGNELGPTLSFKGIDNA
SYYRLMPDQPRYYINDTGTGNTFNLSCPRVLQMVTDSLRYWVAEMKVDGFRFDLATILGREPHGFDEGGGFLDACRQDPL
LASVRLIAEPWDCGPGGYQVGGFPPGWSEWNDTFRDTVRAYWKGDDAMAAELAKRITASGDKFNRRGRRPWASVNFITAH
DGFTLNDLVSYNDKHNEANGEDNRDGHADNRSWNMGVEGPTDDPAIREQRERQKRNLLATLLLSQGTPMILGGDEFGRTQ
RGNNNAYCQDNEISWVDWNGIDADGQALAEFVRKLTTLRRRLPVLRRGRFLTGEHNEALGVTDARWISAAGDDLTQAQWD
DPSMRCFGLVIDGRAQASGIVKAASDATLLLVFNAHFDVVDFTLPAVPDGEHWTCLLDTNVPVRQALPEFPSGAVYQVTG
RSLLLFALEAPSAATQRVFDRLETVLVDGAD

Sequences:

>Translated_751_residues
MSTTPSLQQPGLRDALAAPPDSAGRFGRAIQEGLPFPLGATWNGHGVNFALFSAHATKVELCLFDEDGKTERERIALPEY
TDEVFHVFVPGLEPGAVYGYRVHGPYEPERGHRFNPNKLLLDPYAKAHVGELVWAPEIFGYTLDSPDGDLSFDERDSAPF
VPKCKVVDSTFSWTHPDRHVVAPDRVIVYEAHVRGFTKMHPDLPEPLRGTFAGFAHPRVIDYIRGLGVTTVELLPVHTFV
NDSYLLDKGLTNYWGYNTIGFFAADPRYFADKKASLGEFKEMVDRYHQAGLEVILDVVYNHTAEGNELGPTLSFKGIDNA
SYYRLMPDQPRYYINDTGTGNTFNLSCPRVLQMVTDSLRYWVAEMKVDGFRFDLATILGREPHGFDEGGGFLDACRQDPL
LASVRLIAEPWDCGPGGYQVGGFPPGWSEWNDTFRDTVRAYWKGDDAMAAELAKRITASGDKFNRRGRRPWASVNFITAH
DGFTLNDLVSYNDKHNEANGEDNRDGHADNRSWNMGVEGPTDDPAIREQRERQKRNLLATLLLSQGTPMILGGDEFGRTQ
RGNNNAYCQDNEISWVDWNGIDADGQALAEFVRKLTTLRRRLPVLRRGRFLTGEHNEALGVTDARWISAAGDDLTQAQWD
DPSMRCFGLVIDGRAQASGIVKAASDATLLLVFNAHFDVVDFTLPAVPDGEHWTCLLDTNVPVRQALPEFPSGAVYQVTG
RSLLLFALEAPSAATQRVFDRLETVLVDGAD
>Mature_750_residues
STTPSLQQPGLRDALAAPPDSAGRFGRAIQEGLPFPLGATWNGHGVNFALFSAHATKVELCLFDEDGKTERERIALPEYT
DEVFHVFVPGLEPGAVYGYRVHGPYEPERGHRFNPNKLLLDPYAKAHVGELVWAPEIFGYTLDSPDGDLSFDERDSAPFV
PKCKVVDSTFSWTHPDRHVVAPDRVIVYEAHVRGFTKMHPDLPEPLRGTFAGFAHPRVIDYIRGLGVTTVELLPVHTFVN
DSYLLDKGLTNYWGYNTIGFFAADPRYFADKKASLGEFKEMVDRYHQAGLEVILDVVYNHTAEGNELGPTLSFKGIDNAS
YYRLMPDQPRYYINDTGTGNTFNLSCPRVLQMVTDSLRYWVAEMKVDGFRFDLATILGREPHGFDEGGGFLDACRQDPLL
ASVRLIAEPWDCGPGGYQVGGFPPGWSEWNDTFRDTVRAYWKGDDAMAAELAKRITASGDKFNRRGRRPWASVNFITAHD
GFTLNDLVSYNDKHNEANGEDNRDGHADNRSWNMGVEGPTDDPAIREQRERQKRNLLATLLLSQGTPMILGGDEFGRTQR
GNNNAYCQDNEISWVDWNGIDADGQALAEFVRKLTTLRRRLPVLRRGRFLTGEHNEALGVTDARWISAAGDDLTQAQWDD
PSMRCFGLVIDGRAQASGIVKAASDATLLLVFNAHFDVVDFTLPAVPDGEHWTCLLDTNVPVRQALPEFPSGAVYQVTGR
SLLLFALEAPSAATQRVFDRLETVLVDGAD

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=208, Percent_Identity=28.3653846153846, Blast_Score=78, Evalue=3e-14,
Organism=Escherichia coli, GI2367229, Length=654, Percent_Identity=44.4954128440367, Blast_Score=545, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6320826, Length=225, Percent_Identity=27.1111111111111, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 83641; Mature: 83510

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTPSLQQPGLRDALAAPPDSAGRFGRAIQEGLPFPLGATWNGHGVNFALFSAHATKVE
CCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCEEE
LCLFDEDGKTERERIALPEYTDEVFHVFVPGLEPGAVYGYRVHGPYEPERGHRFNPNKLL
EEEECCCCCCHHHEECCCCHHCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCEEE
LDPYAKAHVGELVWAPEIFGYTLDSPDGDLSFDERDSAPFVPKCKVVDSTFSWTHPDRHV
ECCHHHHHHHHEEECCHHHCEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEE
VAPDRVIVYEAHVRGFTKMHPDLPEPLRGTFAGFAHPRVIDYIRGLGVTTVELLPVHTFV
ECCCEEEEEEHHHCCHHHCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEECC
NDSYLLDKGLTNYWGYNTIGFFAADPRYFADKKASLGEFKEMVDRYHQAGLEVILDVVYN
CCCEEECCCCHHHCCCCCEEEEECCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
HTAEGNELGPTLSFKGIDNASYYRLMPDQPRYYINDTGTGNTFNLSCPRVLQMVTDSLRY
CCCCCCCCCCEEEECCCCCCCEEEECCCCCCEEECCCCCCCEEECCHHHHHHHHHHHHHH
WVAEMKVDGFRFDLATILGREPHGFDEGGGFLDACRQDPLLASVRLIAEPWDCGPGGYQV
HHHHHEECCEEEEHHHHHCCCCCCCCCCCCHHHHHCCCCHHEEHHEEECCCCCCCCCEEC
GGFPPGWSEWNDTFRDTVRAYWKGDDAMAAELAKRITASGDKFNRRGRRPWASVNFITAH
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCEEEEEEEC
DGFTLNDLVSYNDKHNEANGEDNRDGHADNRSWNMGVEGPTDDPAIREQRERQKRNLLAT
CCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHH
LLLSQGTPMILGGDEFGRTQRGNNNAYCQDNEISWVDWNGIDADGQALAEFVRKLTTLRR
HHHCCCCCEEECCHHHCCCCCCCCCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
RLPVLRRGRFLTGEHNEALGVTDARWISAAGDDLTQAQWDDPSMRCFGLVIDGRAQASGI
HCCHHHCCCEECCCCCCCCCCCCHHHEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCE
VKAASDATLLLVFNAHFDVVDFTLPAVPDGEHWTCLLDTNVPVRQALPEFPSGAVYQVTG
EEECCCCEEEEEEECCCCEEEEEECCCCCCCCEEEEEECCCCHHHHCCCCCCCCEEEECC
RSLLLFALEAPSAATQRVFDRLETVLVDGAD
CEEEEEEECCCCHHHHHHHHHHHHHEECCCC
>Mature Secondary Structure 
STTPSLQQPGLRDALAAPPDSAGRFGRAIQEGLPFPLGATWNGHGVNFALFSAHATKVE
CCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCEEE
LCLFDEDGKTERERIALPEYTDEVFHVFVPGLEPGAVYGYRVHGPYEPERGHRFNPNKLL
EEEECCCCCCHHHEECCCCHHCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCEEE
LDPYAKAHVGELVWAPEIFGYTLDSPDGDLSFDERDSAPFVPKCKVVDSTFSWTHPDRHV
ECCHHHHHHHHEEECCHHHCEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEE
VAPDRVIVYEAHVRGFTKMHPDLPEPLRGTFAGFAHPRVIDYIRGLGVTTVELLPVHTFV
ECCCEEEEEEHHHCCHHHCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEECC
NDSYLLDKGLTNYWGYNTIGFFAADPRYFADKKASLGEFKEMVDRYHQAGLEVILDVVYN
CCCEEECCCCHHHCCCCCEEEEECCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
HTAEGNELGPTLSFKGIDNASYYRLMPDQPRYYINDTGTGNTFNLSCPRVLQMVTDSLRY
CCCCCCCCCCEEEECCCCCCCEEEECCCCCCEEECCCCCCCEEECCHHHHHHHHHHHHHH
WVAEMKVDGFRFDLATILGREPHGFDEGGGFLDACRQDPLLASVRLIAEPWDCGPGGYQV
HHHHHEECCEEEEHHHHHCCCCCCCCCCCCHHHHHCCCCHHEEHHEEECCCCCCCCCEEC
GGFPPGWSEWNDTFRDTVRAYWKGDDAMAAELAKRITASGDKFNRRGRRPWASVNFITAH
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCEEEEEEEC
DGFTLNDLVSYNDKHNEANGEDNRDGHADNRSWNMGVEGPTDDPAIREQRERQKRNLLAT
CCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHH
LLLSQGTPMILGGDEFGRTQRGNNNAYCQDNEISWVDWNGIDADGQALAEFVRKLTTLRR
HHHCCCCCEEECCHHHCCCCCCCCCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
RLPVLRRGRFLTGEHNEALGVTDARWISAAGDDLTQAQWDDPSMRCFGLVIDGRAQASGI
HCCHHHCCCEECCCCCCCCCCCCHHHEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCE
VKAASDATLLLVFNAHFDVVDFTLPAVPDGEHWTCLLDTNVPVRQALPEFPSGAVYQVTG
EEECCCCEEEEEEECCCCEEEEEECCCCCCCCEEEEEECCCCHHHHCCCCCCCCEEEECC
RSLLLFALEAPSAATQRVFDRLETVLVDGAD
CEEEEEEECCCCHHHHHHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]