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The map label for this gene is bkdB [H]

Identifier: 238023734

GI number: 238023734

Start: 312147

End: 313511

Strand: Direct

Name: bkdB [H]

Synonym: bglu_2g02660

Alternate gene names: 238023734

Gene position: 312147-313511 (Clockwise)

Preceding gene: 238023733

Following gene: 238023735

Centisome position: 11.04

GC content: 73.77

Gene sequence:

>1365_bases
ATGGGTATCCATGTGATCAAGATGCCGGACATCGGCGAAGGCATCGCGGAAGTCGAGCTGGGGCTGTGGCACGTGAAGGT
GGGCGACCGCGTCGTGGAGGACCAGGCGCTCGCCGACGTGATGACCGACAAGGCGTCGGTGGAGATCCCGTCGCCGGTGG
CCGGGGTGGTCGCCGCGCTCGGCGGCGCCGCCGGCGACATGATGGCGGTGGGCGCGGAACTGATCCGCATCGAGGTGGAC
GGCAGCGGCAACCATCGCGGCGAGCCGGCGGGGGCCGCGAACGAGGCGGTGCCGGCCGCGCCGGCGGCGAGCGCAGCGGT
GCGCGAACCGGCGCACGAGCCGAAGCGCGAGCCTGCTCGTGAAGCCGCGCGTGAACCCGCGCATGCCGCCGCGTGCGCGC
CGGCGCCGGCCGCGCCGATGCCGATGGCCAGGGGCGGCGAGCACGCGGCCACGGCCGCGCCGCCCGTCGCGCGCGAGCCC
GGCGAGCGGCCGCTCGCCTCGCCGGCGGTGCGCAAGCGCGCCTGGGATCTCGGCATCGAGCTGCGCTACGTGCGCGGCAC
GGGCGAGGCGGCGCGCATCCTGCACGAGGATCTGGATGCCTACCTGCAGGGCCGTGGCGCCGCGGCGGCCGTCGGCCGCG
GCGTGCGCGCCGGCTATGCCGAGCGCCACGACGAGGAGGCGGTGCAGGTGATCGGCCTGCGCCGCAGGATCGCCGAGAAG
ATGCAGGAGGCGAAGCGGCGCATCCCGCACTTCAGCTACGTCGAGGAGATCGACGTGACCGAGCTCGACACGCTGCGCGC
CGAGCTGAACCGCCGCCACGGCGAGCAGCGCGGCAAGCTGACGATGCTGCCGTTCATCGCGCGCGCGATGGTGATCGCCC
TGGCCGAGTTCCCGCAGATCAACGCGCGCTACGACGACGAGGCCGGCGTGGTCACGCGCCACGGCGCCGTGCATCTGGGC
GTGGCCACGCAGAGCAAGGCCGGGCTGATGGTGCCGGTGGTGCGCCATGCGGAGGCGCGCGACATCTGGGGGCTCGCGGC
CGAGGTCGCGCGGCTGGCCGAGGCGGTGCGCGGCGGCCGCGCGGCGCGCGACGAGCTGAGCGGCTCGACCATCACGCTGT
CGAGCCTGGGCGCGCTGGGCGGCGTGGTCTCCACGCCGGTGATCAACCGCCCCGAGGTGGGCATCGTCGGTGTGAATCGC
ATCGTCGAGCGGCCGATGATCCGCCACGGGCTGGTGGTCGCGCGCAAGCTGATGAACCTGTCCTCGTCGTTCGATCACCG
CGTGGTGGACGGCATGGATGCCGCCGAATTCATTCAGGCCGTGCGCGCGCTGCTGGAGCAGCCGGCCCTGCTTTTCGTGG
ACTGA

Upstream 100 bases:

>100_bases
ACCGGCTGGGACACGCCTTATCCGCATGCGCAGGAGTGGGCGTACTTTCCGGGGCCGAGCCGCGTGGGCGAGGCGATGCG
ACGCGTGATGGAGAGCTGAG

Downstream 100 bases:

>100_bases
GCCGATGAACGAACCGATCACAACCACGCTGCTCGTCATCGGCGCCGGCCCGGGCGGCTACGTCGCCGCGATCCGCGCCG
CCCAGCTGGGCGTCCCCACC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MGIHVIKMPDIGEGIAEVELGLWHVKVGDRVVEDQALADVMTDKASVEIPSPVAGVVAALGGAAGDMMAVGAELIRIEVD
GSGNHRGEPAGAANEAVPAAPAASAAVREPAHEPKREPAREAAREPAHAAACAPAPAAPMPMARGGEHAATAAPPVAREP
GERPLASPAVRKRAWDLGIELRYVRGTGEAARILHEDLDAYLQGRGAAAAVGRGVRAGYAERHDEEAVQVIGLRRRIAEK
MQEAKRRIPHFSYVEEIDVTELDTLRAELNRRHGEQRGKLTMLPFIARAMVIALAEFPQINARYDDEAGVVTRHGAVHLG
VATQSKAGLMVPVVRHAEARDIWGLAAEVARLAEAVRGGRAARDELSGSTITLSSLGALGGVVSTPVINRPEVGIVGVNR
IVERPMIRHGLVVARKLMNLSSSFDHRVVDGMDAAEFIQAVRALLEQPALLFVD

Sequences:

>Translated_454_residues
MGIHVIKMPDIGEGIAEVELGLWHVKVGDRVVEDQALADVMTDKASVEIPSPVAGVVAALGGAAGDMMAVGAELIRIEVD
GSGNHRGEPAGAANEAVPAAPAASAAVREPAHEPKREPAREAAREPAHAAACAPAPAAPMPMARGGEHAATAAPPVAREP
GERPLASPAVRKRAWDLGIELRYVRGTGEAARILHEDLDAYLQGRGAAAAVGRGVRAGYAERHDEEAVQVIGLRRRIAEK
MQEAKRRIPHFSYVEEIDVTELDTLRAELNRRHGEQRGKLTMLPFIARAMVIALAEFPQINARYDDEAGVVTRHGAVHLG
VATQSKAGLMVPVVRHAEARDIWGLAAEVARLAEAVRGGRAARDELSGSTITLSSLGALGGVVSTPVINRPEVGIVGVNR
IVERPMIRHGLVVARKLMNLSSSFDHRVVDGMDAAEFIQAVRALLEQPALLFVD
>Mature_453_residues
GIHVIKMPDIGEGIAEVELGLWHVKVGDRVVEDQALADVMTDKASVEIPSPVAGVVAALGGAAGDMMAVGAELIRIEVDG
SGNHRGEPAGAANEAVPAAPAASAAVREPAHEPKREPAREAAREPAHAAACAPAPAAPMPMARGGEHAATAAPPVAREPG
ERPLASPAVRKRAWDLGIELRYVRGTGEAARILHEDLDAYLQGRGAAAAVGRGVRAGYAERHDEEAVQVIGLRRRIAEKM
QEAKRRIPHFSYVEEIDVTELDTLRAELNRRHGEQRGKLTMLPFIARAMVIALAEFPQINARYDDEAGVVTRHGAVHLGV
ATQSKAGLMVPVVRHAEARDIWGLAAEVARLAEAVRGGRAARDELSGSTITLSSLGALGGVVSTPVINRPEVGIVGVNRI
VERPMIRHGLVVARKLMNLSSSFDHRVVDGMDAAEFIQAVRALLEQPALLFVD

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=469, Percent_Identity=30.0639658848614, Blast_Score=196, Evalue=5e-50,
Organism=Homo sapiens, GI31711992, Length=442, Percent_Identity=27.3755656108597, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI19923748, Length=248, Percent_Identity=30.6451612903226, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI203098816, Length=227, Percent_Identity=28.1938325991189, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI203098753, Length=227, Percent_Identity=28.1938325991189, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI260898739, Length=162, Percent_Identity=29.0123456790123, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1786946, Length=456, Percent_Identity=26.0964912280702, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1786305, Length=294, Percent_Identity=29.9319727891156, Blast_Score=155, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17537937, Length=464, Percent_Identity=31.8965517241379, Blast_Score=211, Evalue=9e-55,
Organism=Caenorhabditis elegans, GI25146366, Length=452, Percent_Identity=27.4336283185841, Blast_Score=158, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17560088, Length=451, Percent_Identity=28.3813747228381, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17538894, Length=235, Percent_Identity=25.1063829787234, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320352, Length=228, Percent_Identity=29.3859649122807, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324258, Length=472, Percent_Identity=24.5762711864407, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24645909, Length=231, Percent_Identity=29.004329004329, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=28.9473684210526, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=28.9473684210526, Blast_Score=108, Evalue=6e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 48137; Mature: 48006

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIHVIKMPDIGEGIAEVELGLWHVKVGDRVVEDQALADVMTDKASVEIPSPVAGVVAAL
CCEEEEECCCCCCCHHHHHHEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
GGAAGDMMAVGAELIRIEVDGSGNHRGEPAGAANEAVPAAPAASAAVREPAHEPKREPAR
CCCCHHHHHHCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHH
EAAREPAHAAACAPAPAAPMPMARGGEHAATAAPPVAREPGERPLASPAVRKRAWDLGIE
HHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCE
LRYVRGTGEAARILHEDLDAYLQGRGAAAAVGRGVRAGYAERHDEEAVQVIGLRRRIAEK
EEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHH
MQEAKRRIPHFSYVEEIDVTELDTLRAELNRRHGEQRGKLTMLPFIARAMVIALAEFPQI
HHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHCCHHCCCEEHHHHHHHHHHHHHHHCCCC
NARYDDEAGVVTRHGAVHLGVATQSKAGLMVPVVRHAEARDIWGLAAEVARLAEAVRGGR
CCCCCCCCCEEEECCEEEEEEECCCCCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
AARDELSGSTITLSSLGALGGVVSTPVINRPEVGIVGVNRIVERPMIRHGLVVARKLMNL
HHHHHCCCCEEEHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
SSSFDHRVVDGMDAAEFIQAVRALLEQPALLFVD
HCCCCHHHHCCCCHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure 
GIHVIKMPDIGEGIAEVELGLWHVKVGDRVVEDQALADVMTDKASVEIPSPVAGVVAAL
CEEEEECCCCCCCHHHHHHEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
GGAAGDMMAVGAELIRIEVDGSGNHRGEPAGAANEAVPAAPAASAAVREPAHEPKREPAR
CCCCHHHHHHCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHH
EAAREPAHAAACAPAPAAPMPMARGGEHAATAAPPVAREPGERPLASPAVRKRAWDLGIE
HHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCE
LRYVRGTGEAARILHEDLDAYLQGRGAAAAVGRGVRAGYAERHDEEAVQVIGLRRRIAEK
EEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHH
MQEAKRRIPHFSYVEEIDVTELDTLRAELNRRHGEQRGKLTMLPFIARAMVIALAEFPQI
HHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHCCHHCCCEEHHHHHHHHHHHHHHHCCCC
NARYDDEAGVVTRHGAVHLGVATQSKAGLMVPVVRHAEARDIWGLAAEVARLAEAVRGGR
CCCCCCCCCEEEECCEEEEEEECCCCCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
AARDELSGSTITLSSLGALGGVVSTPVINRPEVGIVGVNRIVERPMIRHGLVVARKLMNL
HHHHHCCCCEEEHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
SSSFDHRVVDGMDAAEFIQAVRALLEQPALLFVD
HCCCCHHHHCCCCHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 3046941 [H]