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The map label for this gene is lpdV [H]

Identifier: 238023735

GI number: 238023735

Start: 313516

End: 314913

Strand: Direct

Name: lpdV [H]

Synonym: bglu_2g02670

Alternate gene names: 238023735

Gene position: 313516-314913 (Clockwise)

Preceding gene: 238023734

Following gene: 238023736

Centisome position: 11.09

GC content: 74.25

Gene sequence:

>1398_bases
ATGAACGAACCGATCACAACCACGCTGCTCGTCATCGGCGCCGGCCCGGGCGGCTACGTCGCCGCGATCCGCGCCGCCCA
GCTGGGCGTCCCCACCGTGGTGGTGGAGCGCGCGCAGCCGGGCGGCACCTGCCTGAACATCGGCTGCATCCCGTCCAAGG
CGCTGATCCACGCGGCCGACGAGTTCGACAAGGCGCGCCACTACAGCGGCGCTTCCCCGCTCGGCATCCGCGTGGCCTCG
CCCGAGATCGACATCGCGCGCACCGTCGCCTGGAAGGACGGCATCGTCGCGAAGCTGACGGGCGGCGTGGCCGCGCTGCT
CGCGCGCCACGGCGTCGAGCTGGTGCGCGGCGACGCGCGCGTGCTCGACGGCAAGACCGTGGAGGTGGTGCGCGACGCCG
CCCGCCCGCTGCGCATCCGCTGCGAGCACCTGCTGCTCGCGGCCGGCTCCGAGCCCGTCGCGCTGCCGTCGATGCCGTTC
GGCGGCATCGTGCAATCGTCCACCGAGGCGCTGTCGCCCGCCACGCTGCCGCGCCGGCTGGTGGTGGTGGGCGCCGGCTA
CATCGGGCTCGAGCTGGCGATCGCCTACCGCAAGCTCGGTGCCGAGGTGAGCGTGGTCGAGGCCCAGGAGCGCATCCTGC
CGGCCTACGACGCGGCGCTGACCAAGCCGGTGGCGGCCGCGCTCGCGCGGCTCGGCATCACGCTGCAGCTCGGCCGCAAG
GTGCTGGGCCTGAACCCGGCCGGCGACGCGGTGCGGGTGCAGGATGCCGCCGGCGCCGAGACGGCGCTGCCGGCCGACCG
CGTGCTGGTGGCGGTGGGGCGCCGGCCGCGCACCCAGGGCTGGGGGCTCGAGGCGCTGCAGCTCGATCGCGCCGGCCACG
CGCTGAAGGTGGACGACCAGTGCCGGACCTCGATGCGCAACGTCTGGGCGATCGGCGATCTGGCGGGCGAGCCGATGCTC
GCGCATCGCGCGATGGCACAGGGCGAGATGGTGGCCGAGATCGTCTCGGGCAAGCGGCGGCGCTTCATGCCGGCGGCGAT
CCCGGCGATCTGCTTCACCGATCCCGAGGTGGTGTCGGTGGGGCTCGCGCCGCACGATGCGGCCGCGCCGGCCGACGCGC
TGGTGGCCAGCTTTCCGCTGATGGCCAACGGCCGCGCGATGACGATCGAGGGCACCGACGGCTTCGTGCGCGTGGTGGCG
CGTCGCGACAATCATCTGATCCTCGGCTGGCAGGCGGTGGGCCGCGGCGTGTCCGAACTCGCGGCGGCGTTCTCGCAATC
GATCGAGATGGGCGCGCGGCTCGAGGACGTGGGCGGCACCATCCACGCCCATCCGACGCTCGGCGAGGCGGTGCAGGAAG
CGGCGCTGCGCGCGCTCGGCCACGCGCTGCATGTCTGA

Upstream 100 bases:

>100_bases
TTCGATCACCGCGTGGTGGACGGCATGGATGCCGCCGAATTCATTCAGGCCGTGCGCGCGCTGCTGGAGCAGCCGGCCCT
GCTTTTCGTGGACTGAGCCG

Downstream 100 bases:

>100_bases
GCGGCGGCGACGATGCCGCGCGCGAGCATGCGCGGCATCCCCGCAGCACACGATCAACCACACCAGGCCGGGAAGCCAGA
CTGACCCGGCCATCAGGCAT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MNEPITTTLLVIGAGPGGYVAAIRAAQLGVPTVVVERAQPGGTCLNIGCIPSKALIHAADEFDKARHYSGASPLGIRVAS
PEIDIARTVAWKDGIVAKLTGGVAALLARHGVELVRGDARVLDGKTVEVVRDAARPLRIRCEHLLLAAGSEPVALPSMPF
GGIVQSSTEALSPATLPRRLVVVGAGYIGLELAIAYRKLGAEVSVVEAQERILPAYDAALTKPVAAALARLGITLQLGRK
VLGLNPAGDAVRVQDAAGAETALPADRVLVAVGRRPRTQGWGLEALQLDRAGHALKVDDQCRTSMRNVWAIGDLAGEPML
AHRAMAQGEMVAEIVSGKRRRFMPAAIPAICFTDPEVVSVGLAPHDAAAPADALVASFPLMANGRAMTIEGTDGFVRVVA
RRDNHLILGWQAVGRGVSELAAAFSQSIEMGARLEDVGGTIHAHPTLGEAVQEAALRALGHALHV

Sequences:

>Translated_465_residues
MNEPITTTLLVIGAGPGGYVAAIRAAQLGVPTVVVERAQPGGTCLNIGCIPSKALIHAADEFDKARHYSGASPLGIRVAS
PEIDIARTVAWKDGIVAKLTGGVAALLARHGVELVRGDARVLDGKTVEVVRDAARPLRIRCEHLLLAAGSEPVALPSMPF
GGIVQSSTEALSPATLPRRLVVVGAGYIGLELAIAYRKLGAEVSVVEAQERILPAYDAALTKPVAAALARLGITLQLGRK
VLGLNPAGDAVRVQDAAGAETALPADRVLVAVGRRPRTQGWGLEALQLDRAGHALKVDDQCRTSMRNVWAIGDLAGEPML
AHRAMAQGEMVAEIVSGKRRRFMPAAIPAICFTDPEVVSVGLAPHDAAAPADALVASFPLMANGRAMTIEGTDGFVRVVA
RRDNHLILGWQAVGRGVSELAAAFSQSIEMGARLEDVGGTIHAHPTLGEAVQEAALRALGHALHV
>Mature_465_residues
MNEPITTTLLVIGAGPGGYVAAIRAAQLGVPTVVVERAQPGGTCLNIGCIPSKALIHAADEFDKARHYSGASPLGIRVAS
PEIDIARTVAWKDGIVAKLTGGVAALLARHGVELVRGDARVLDGKTVEVVRDAARPLRIRCEHLLLAAGSEPVALPSMPF
GGIVQSSTEALSPATLPRRLVVVGAGYIGLELAIAYRKLGAEVSVVEAQERILPAYDAALTKPVAAALARLGITLQLGRK
VLGLNPAGDAVRVQDAAGAETALPADRVLVAVGRRPRTQGWGLEALQLDRAGHALKVDDQCRTSMRNVWAIGDLAGEPML
AHRAMAQGEMVAEIVSGKRRRFMPAAIPAICFTDPEVVSVGLAPHDAAAPADALVASFPLMANGRAMTIEGTDGFVRVVA
RRDNHLILGWQAVGRGVSELAAAFSQSIEMGARLEDVGGTIHAHPTLGEAVQEAALRALGHALHV

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=467, Percent_Identity=35.3319057815846, Blast_Score=262, Evalue=5e-70,
Organism=Homo sapiens, GI50301238, Length=465, Percent_Identity=27.0967741935484, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI22035672, Length=458, Percent_Identity=27.9475982532751, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI291045266, Length=463, Percent_Identity=22.6781857451404, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI33519430, Length=457, Percent_Identity=22.1006564551422, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI33519428, Length=457, Percent_Identity=22.1006564551422, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI33519426, Length=457, Percent_Identity=22.1006564551422, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI148277071, Length=457, Percent_Identity=22.1006564551422, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI148277065, Length=457, Percent_Identity=22.1006564551422, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1786307, Length=462, Percent_Identity=37.4458874458874, Blast_Score=295, Evalue=5e-81,
Organism=Escherichia coli, GI87082354, Length=465, Percent_Identity=28.8172043010753, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1789915, Length=439, Percent_Identity=27.3348519362187, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI87081717, Length=457, Percent_Identity=25.8205689277899, Blast_Score=119, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32565766, Length=458, Percent_Identity=36.6812227074236, Blast_Score=268, Evalue=5e-72,
Organism=Caenorhabditis elegans, GI71983419, Length=441, Percent_Identity=24.9433106575964, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI71983429, Length=441, Percent_Identity=24.9433106575964, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17557007, Length=462, Percent_Identity=22.7272727272727, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321091, Length=473, Percent_Identity=35.5179704016913, Blast_Score=248, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6325166, Length=459, Percent_Identity=26.5795206971678, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6325240, Length=470, Percent_Identity=25.9574468085106, Blast_Score=141, Evalue=3e-34,
Organism=Drosophila melanogaster, GI21358499, Length=460, Percent_Identity=38.4782608695652, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24640549, Length=472, Percent_Identity=25.2118644067797, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24640553, Length=480, Percent_Identity=25, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24640551, Length=472, Percent_Identity=25.2118644067797, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI17737741, Length=466, Percent_Identity=25.5364806866953, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48440; Mature: 48440

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEPITTTLLVIGAGPGGYVAAIRAAQLGVPTVVVERAQPGGTCLNIGCIPSKALIHAAD
CCCCCEEEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCHHHHHHHH
EFDKARHYSGASPLGIRVASPEIDIARTVAWKDGIVAKLTGGVAALLARHGVELVRGDAR
HHHHHHHCCCCCCCEEEEECCCCCEEEEEEECCCEEEEHHHHHHHHHHHCCHHHCCCCCE
VLDGKTVEVVRDAARPLRIRCEHLLLAAGSEPVALPSMPFGGIVQSSTEALSPATLPRRL
EECCCHHHHHHHCCCCHHEEEEEEEEECCCCCCCCCCCCCCCHHCCCHHHCCCCCCCCEE
VVVGAGYIGLELAIAYRKLGAEVSVVEAQERILPAYDAALTKPVAAALARLGITLQLGRK
EEEECCHHHHHHHHHHHHHCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCE
VLGLNPAGDAVRVQDAAGAETALPADRVLVAVGRRPRTQGWGLEALQLDRAGHALKVDDQ
EECCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEECHH
CRTSMRNVWAIGDLAGEPMLAHRAMAQGEMVAEIVSGKRRRFMPAAIPAICFTDPEVVSV
HHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCHHCCEEECCCCHHEEE
GLAPHDAAAPADALVASFPLMANGRAMTIEGTDGFVRVVARRDNHLILGWQAVGRGVSEL
ECCCCCCCCCHHHHHHHCCEEECCEEEEEECCCCEEEEEEECCCEEEEEHHHHCCCHHHH
AAAFSQSIEMGARLEDVGGTIHAHPTLGEAVQEAALRALGHALHV
HHHHHHHHHHCCEEHHCCCEEEECCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNEPITTTLLVIGAGPGGYVAAIRAAQLGVPTVVVERAQPGGTCLNIGCIPSKALIHAAD
CCCCCEEEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCHHHHHHHH
EFDKARHYSGASPLGIRVASPEIDIARTVAWKDGIVAKLTGGVAALLARHGVELVRGDAR
HHHHHHHCCCCCCCEEEEECCCCCEEEEEEECCCEEEEHHHHHHHHHHHCCHHHCCCCCE
VLDGKTVEVVRDAARPLRIRCEHLLLAAGSEPVALPSMPFGGIVQSSTEALSPATLPRRL
EECCCHHHHHHHCCCCHHEEEEEEEEECCCCCCCCCCCCCCCHHCCCHHHCCCCCCCCEE
VVVGAGYIGLELAIAYRKLGAEVSVVEAQERILPAYDAALTKPVAAALARLGITLQLGRK
EEEECCHHHHHHHHHHHHHCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCE
VLGLNPAGDAVRVQDAAGAETALPADRVLVAVGRRPRTQGWGLEALQLDRAGHALKVDDQ
EECCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEECHH
CRTSMRNVWAIGDLAGEPMLAHRAMAQGEMVAEIVSGKRRRFMPAAIPAICFTDPEVVSV
HHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCHHCCEEECCCCHHEEE
GLAPHDAAAPADALVASFPLMANGRAMTIEGTDGFVRVVARRDNHLILGWQAVGRGVSEL
ECCCCCCCCCHHHHHHHCCEEECCEEEEEECCCCEEEEEEECCCEEEEEHHHHCCCHHHH
AAAFSQSIEMGARLEDVGGTIHAHPTLGEAVQEAALRALGHALHV
HHHHHHHHHHCCEEHHCCCEEEECCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]