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The map label for this gene is prpB [C]

Identifier: 238023674

GI number: 238023674

Start: 222334

End: 223143

Strand: Reverse

Name: prpB [C]

Synonym: bglu_2g01950

Alternate gene names: 238023674

Gene position: 223143-222334 (Counterclockwise)

Preceding gene: 238023676

Following gene: 238023673

Centisome position: 7.89

GC content: 72.72

Gene sequence:

>810_bases
ATGCCCCGCACTCCCGCCGAAAAACGCGCCGCCTTCCGCCAGCTCCACGAAAGCGGCTGCTTCCTGCTGCCCAACCCGTG
GGACGTCGGCAGCGCGCGCTACCTCGCCTCGCTCGGCTTCCAGGCGCTCGCCACCACCAGTTCGGGTTACGCGTGGTCCA
CCGGCCACGCCGACAACACGGTGTCGCGCGCGGCCGTGCTCGCGCATCTGCGCGACATCGTCGGCGCCACGGATCTGCCC
GTGAACGCCGATTTCGAGAACGGTTTCGGCGCGACCCCCGACGAGGTCGGCGAAAGCGTGCGGCTGGCGGTGGAGACCGG
CATCGCCGGGCTCTCGATCGAGGACGCGACGGGCGACACGGCCACCCCGCTGTTCCCCATCGACGAGGCGGTGGCACGCA
TCGCCGCGGCGCGCCGCGCGATCGATGCCTCCGGCGGCGACACGCTGCTGGTGGGCCGCGCCGAGAACTTCGTGGTGGGC
GTGCCCGATCTCGACGACGCGATCGCGCGGCTGCGCGCCTACGCGGCAGCCGGCGCCGACTGCCTCTACGCACCCGGCAT
CCGCACGCGCGGGCAGATCGAGGCGGTGGTGGCCGCGCTCGCGCCCAAGCCGGTCAACCTGCTGGTGGGCGCGCCGATCG
AATTCTCGCTGGCCGAGATCGCCGGGTTCGGGGTGCGCCGCGTAAGCGTGGGCGGAGCGCTCGCGCGCGCGGCCTGGGAC
GGCTTCATGAACGCCACGCTGGGCTTGCTCGACGGCAGCTTCGCGCGGCTGCCGACGCTGTCGGGCAAGCAGATCGACGC
GCAGTTCTGA

Upstream 100 bases:

>100_bases
CGCCGCGCCGCGCGCCGCGCGTCGCCGCTACCGCTCATGCCTCGCCTCAGGATCACGAACCCGGCGCACCGCGCCACGAC
GGACCACCAAGGACATCGAC

Downstream 100 bases:

>100_bases
ACCGCGCGCCGTATCGTGCCGCGCGGCCGCCCGCTCGGCTGCACGAGAGCTTCCCGCAACCGCTCAACATCGGCGAGAGG
GTACGCGCGGCGGGCAGGGA

Product: PEP phosphonomutase

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MPRTPAEKRAAFRQLHESGCFLLPNPWDVGSARYLASLGFQALATTSSGYAWSTGHADNTVSRAAVLAHLRDIVGATDLP
VNADFENGFGATPDEVGESVRLAVETGIAGLSIEDATGDTATPLFPIDEAVARIAAARRAIDASGGDTLLVGRAENFVVG
VPDLDDAIARLRAYAAAGADCLYAPGIRTRGQIEAVVAALAPKPVNLLVGAPIEFSLAEIAGFGVRRVSVGGALARAAWD
GFMNATLGLLDGSFARLPTLSGKQIDAQF

Sequences:

>Translated_269_residues
MPRTPAEKRAAFRQLHESGCFLLPNPWDVGSARYLASLGFQALATTSSGYAWSTGHADNTVSRAAVLAHLRDIVGATDLP
VNADFENGFGATPDEVGESVRLAVETGIAGLSIEDATGDTATPLFPIDEAVARIAAARRAIDASGGDTLLVGRAENFVVG
VPDLDDAIARLRAYAAAGADCLYAPGIRTRGQIEAVVAALAPKPVNLLVGAPIEFSLAEIAGFGVRRVSVGGALARAAWD
GFMNATLGLLDGSFARLPTLSGKQIDAQF
>Mature_268_residues
PRTPAEKRAAFRQLHESGCFLLPNPWDVGSARYLASLGFQALATTSSGYAWSTGHADNTVSRAAVLAHLRDIVGATDLPV
NADFENGFGATPDEVGESVRLAVETGIAGLSIEDATGDTATPLFPIDEAVARIAAARRAIDASGGDTLLVGRAENFVVGV
PDLDDAIARLRAYAAAGADCLYAPGIRTRGQIEAVVAALAPKPVNLLVGAPIEFSLAEIAGFGVRRVSVGGALARAAWDG
FMNATLGLLDGSFARLPTLSGKQIDAQF

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 27860; Mature: 27728

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRTPAEKRAAFRQLHESGCFLLPNPWDVGSARYLASLGFQALATTSSGYAWSTGHADNT
CCCCCHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHCHHHEEECCCCCEEECCCCCCH
VSRAAVLAHLRDIVGATDLPVNADFENGFGATPDEVGESVRLAVETGIAGLSIEDATGDT
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCHHEEEEEECCCCCEEEECCCCCC
ATPLFPIDEAVARIAAARRAIDASGGDTLLVGRAENFVVGVPDLDDAIARLRAYAAAGAD
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHCCCC
CLYAPGIRTRGQIEAVVAALAPKPVNLLVGAPIEFSLAEIAGFGVRRVSVGGALARAAWD
EEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHCCCEEEEECCHHHHHHHHH
GFMNATLGLLDGSFARLPTLSGKQIDAQF
HHHHHHHHHHCCCHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
PRTPAEKRAAFRQLHESGCFLLPNPWDVGSARYLASLGFQALATTSSGYAWSTGHADNT
CCCCHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHCHHHEEECCCCCEEECCCCCCH
VSRAAVLAHLRDIVGATDLPVNADFENGFGATPDEVGESVRLAVETGIAGLSIEDATGDT
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCHHEEEEEECCCCCEEEECCCCCC
ATPLFPIDEAVARIAAARRAIDASGGDTLLVGRAENFVVGVPDLDDAIARLRAYAAAGAD
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHCCCC
CLYAPGIRTRGQIEAVVAALAPKPVNLLVGAPIEFSLAEIAGFGVRRVSVGGALARAAWD
EEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHCCCEEEEECCHHHHHHHHH
GFMNATLGLLDGSFARLPTLSGKQIDAQF
HHHHHHHHHHCCCHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]