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The map label for this gene is vanB [H]

Identifier: 238023598

GI number: 238023598

Start: 105066

End: 106034

Strand: Reverse

Name: vanB [H]

Synonym: bglu_2g01010

Alternate gene names: 238023598

Gene position: 106034-105066 (Counterclockwise)

Preceding gene: 238023603

Following gene: 238023596

Centisome position: 3.75

GC content: 70.49

Gene sequence:

>969_bases
ATGCAAACCTTCACCTTCAAGGTCCGCGTCGACGCGATACGCGACGAAGCCCAGGACATCCGCTCGTTTCGCATCGTCCG
GCTCGACGGCCTGCCGTTCGACGTCCATGAGCCCGGCGCGCACATCGACGTCACCGCGCCGTCGGGGATCACACGACAGT
ATTCGCTGTGCGGCGACCCGGCCGAACGCGGCGCGCAGCTGTTCGCGGTCAAGAAGGAAGCGGCGTCGCGCGGCGGCTCG
CGCTCGCTGCACGACGACGTGGTGGTCGGCTCGGAGCTGATAGTCAGCCCGCCGCGCAACCTGTTCCGGCTCGCCGAGCA
GGCACGCACGCACGTGCTGATCGGCGCCGGCATCGGCATCACGCCGCTGCTGTCGATGGCGTACCGGCTGACGGCGCGCG
GCACGCCGTTCGTGCTGCATTACTTCGCGCGCAGCGAGGCGCATGCCGCTTTCCTGCCGACGCTGTCGCGCGAGCCGCTG
GCACGCCATCTGCGCCTTCACTTCGGCGTCGAACCGGCCGACCTCGAGGCGGCGCTGCACGACTGCCTGAGCGGCGCCGG
GCCCGGCACGCACGTCTATACCTGCGGCCCGGGCCCGTTCATGGACCAGGTGGTGGAAGCCGGCGAGGCGGCGGTCGGCG
CCGACGCCGTGCATCTGGAGCGCTTCGCGGCCGAGCCCGTCGCCGCAGCCGAGGGCACGCTCGACAGCTTCGAGGTGCAG
GTGGCCGGTTCCGGCCGGGTGGTCCAGGTCGGCAAGGGACAGACGATCGTCGGCGCGCTGGCGGAAATCGGCATCGACAT
CGACACATCGTGCGGCGAAGGCGTGTGCGGCACCTGCATCGTCGACGTGGTGGACGGCGAGCCCGACCACCGCGACCACT
GTCTGAGCAAGGCCGAGCGCGCCGCCAACCGGGTCATTTGCTGCTGCGTGTCGCGCTCGCGCTCGAAGCGGATCGTGCTC
GACGTCTGA

Upstream 100 bases:

>100_bases
CAATTAATATCAATTTTGTGAATTCGAAGCTTGGACCTAAGCTTGTGCCAAGCGGTGCGCGCGACGGTACGGCCGCCGAT
CCTTCTCACACCAATTCGCC

Downstream 100 bases:

>100_bases
GCGCGGGCCGGGCGGCCGCCTCCGAGCCTGCGAAGCGCCGCATGATCTGGCCCAGCAGCTGGCGCATCCAGGCAATGCCC
TCGTCCTCGTGAAAATGCTC

Product: oxidoreductase

Products: 3,4-dihydroxybenzoate; NAD+; H2O; formaldehyde

Alternate protein names: Vanillate degradation ferredoxin-like protein [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MQTFTFKVRVDAIRDEAQDIRSFRIVRLDGLPFDVHEPGAHIDVTAPSGITRQYSLCGDPAERGAQLFAVKKEAASRGGS
RSLHDDVVVGSELIVSPPRNLFRLAEQARTHVLIGAGIGITPLLSMAYRLTARGTPFVLHYFARSEAHAAFLPTLSREPL
ARHLRLHFGVEPADLEAALHDCLSGAGPGTHVYTCGPGPFMDQVVEAGEAAVGADAVHLERFAAEPVAAAEGTLDSFEVQ
VAGSGRVVQVGKGQTIVGALAEIGIDIDTSCGEGVCGTCIVDVVDGEPDHRDHCLSKAERAANRVICCCVSRSRSKRIVL
DV

Sequences:

>Translated_322_residues
MQTFTFKVRVDAIRDEAQDIRSFRIVRLDGLPFDVHEPGAHIDVTAPSGITRQYSLCGDPAERGAQLFAVKKEAASRGGS
RSLHDDVVVGSELIVSPPRNLFRLAEQARTHVLIGAGIGITPLLSMAYRLTARGTPFVLHYFARSEAHAAFLPTLSREPL
ARHLRLHFGVEPADLEAALHDCLSGAGPGTHVYTCGPGPFMDQVVEAGEAAVGADAVHLERFAAEPVAAAEGTLDSFEVQ
VAGSGRVVQVGKGQTIVGALAEIGIDIDTSCGEGVCGTCIVDVVDGEPDHRDHCLSKAERAANRVICCCVSRSRSKRIVL
DV
>Mature_322_residues
MQTFTFKVRVDAIRDEAQDIRSFRIVRLDGLPFDVHEPGAHIDVTAPSGITRQYSLCGDPAERGAQLFAVKKEAASRGGS
RSLHDDVVVGSELIVSPPRNLFRLAEQARTHVLIGAGIGITPLLSMAYRLTARGTPFVLHYFARSEAHAAFLPTLSREPL
ARHLRLHFGVEPADLEAALHDCLSGAGPGTHVYTCGPGPFMDQVVEAGEAAVGADAVHLERFAAEPVAAAEGTLDSFEVQ
VAGSGRVVQVGKGQTIVGALAEIGIDIDTSCGEGVCGTCIVDVVDGEPDHRDHCLSKAERAANRVICCCVSRSRSKRIVL
DV

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=322, Percent_Identity=37.2670807453416, Blast_Score=197, Evalue=6e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.14.13.82

Molecular weight: Translated: 34292; Mature: 34292

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTFTFKVRVDAIRDEAQDIRSFRIVRLDGLPFDVHEPGAHIDVTAPSGITRQYSLCGDP
CCEEEEEEEEHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEEEEECCCCCCEEECCCCCH
AERGAQLFAVKKEAASRGGSRSLHDDVVVGSELIVSPPRNLFRLAEQARTHVLIGAGIGI
HHCCCEEEEEEHHHHHCCCCCCCCCCEEECCEEEECCCHHHHHHHHHCCCEEEEECCCCH
TPLLSMAYRLTARGTPFVLHYFARSEAHAAFLPTLSREPLARHLRLHFGVEPADLEAALH
HHHHHHHHHHHCCCCCEEEEEEHHCCCCEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHH
DCLSGAGPGTHVYTCGPGPFMDQVVEAGEAAVGADAVHLERFAAEPVAAAEGTLDSFEVQ
HHHCCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHCCCCCCCEEEE
VAGSGRVVQVGKGQTIVGALAEIGIDIDTSCGEGVCGTCIVDVVDGEPDHRDHCLSKAER
ECCCCCEEEECCCCCHHHHHHHHCCCEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH
AANRVICCCVSRSRSKRIVLDV
HHCCEEEEEECCCCCCEEEEEC
>Mature Secondary Structure
MQTFTFKVRVDAIRDEAQDIRSFRIVRLDGLPFDVHEPGAHIDVTAPSGITRQYSLCGDP
CCEEEEEEEEHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEEEEECCCCCCEEECCCCCH
AERGAQLFAVKKEAASRGGSRSLHDDVVVGSELIVSPPRNLFRLAEQARTHVLIGAGIGI
HHCCCEEEEEEHHHHHCCCCCCCCCCEEECCEEEECCCHHHHHHHHHCCCEEEEECCCCH
TPLLSMAYRLTARGTPFVLHYFARSEAHAAFLPTLSREPLARHLRLHFGVEPADLEAALH
HHHHHHHHHHHCCCCCEEEEEEHHCCCCEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHH
DCLSGAGPGTHVYTCGPGPFMDQVVEAGEAAVGADAVHLERFAAEPVAAAEGTLDSFEVQ
HHHCCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHCCCCCCCEEEE
VAGSGRVVQVGKGQTIVGALAEIGIDIDTSCGEGVCGTCIVDVVDGEPDHRDHCLSKAER
ECCCCCEEEECCCCCHHHHHHHHCCCEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH
AANRVICCCVSRSRSKRIVLDV
HHCCEEEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vanillate; O2; NADH; H+

Specific reaction: vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9098058 [H]