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The map label for this gene is xylX [H]

Identifier: 238023596

GI number: 238023596

Start: 102702

End: 103979

Strand: Reverse

Name: xylX [H]

Synonym: bglu_2g00990

Alternate gene names: 238023596

Gene position: 103979-102702 (Counterclockwise)

Preceding gene: 238023598

Following gene: 238023595

Centisome position: 3.68

GC content: 66.2

Gene sequence:

>1278_bases
ATGAGTGAAACCTGGCAAAGCATCGATACGCGTGCACTGCGCGGCCGCGCGGAAGCCGACCGTATCGCCCCGTCGCTCTA
CTACGATCCGCAGATCTTCGAGGCCGAGCTCGAGCGCATCTTCTACAAGACCTGGATCTGGGTCGCGCACGAAAGCGAGC
TGCCGCAGCCCGGCGACTTCCGCACCACCACGATCGGCCGCCAGCCGGTGATCGTGGTGCGCGACAGGACGGGCACCGTC
AACGTGCTGCAGAACCGCTGCCGCCACCGCGGCGCGACGGTCTGCGAGGAGCACCAGGGCAACGCAAAGGGCTTCACCTG
CCCGTACCACAGCTGGTCCTACGCGCTCGACGGCGCGCTGCGCGCGCTGCCATACGGCGACGGCTACGAGGGCGTGTGCG
AGAAGGGCGACCTGCCGCTCGAAAAGCTGCGCGTCGGCATCTATCAAGGCCTGATCTTCGCGAGCTTCAATCAGGAAATC
GAACCGCTCGAGGATTTCCTCGGCGGCGCCAAGCCCTGGATCGACCTGTTCATGAAGCAGGGCGCCGGCTACCCGATCAA
GTCGAACGGCGAGCACAAGTTCCGCTTCAAGGGCAACTGGAAGATCCAGCTCGAGAACACCACCGACCTGTATCACTTCC
CGGTGGTGCACAAGTCGTGGATGAAATCGATCGACGAACAGACGGCCGAGGTGATCACCCGCTTCATGACCAGCGACAAG
GCATTCTGCCGGTCGCTGGGCAACGGCCACAGCCTGGCCGTGCTGGTGCCCGAAATCGTCGACCTCGACGTGGACGACGG
CGCGCCGCTGCCCGAGCGATACGACGCGCTGGTCGCGCAACTGTCCGCAAGGCACGCGCCGGAGGAGGTGCGCCGGATCG
TGCGCTCGCTGATGGGCGTCGGCTTCAACCTGAACCTGTTCCCGAACCTCGCGCTGTCGATGGCGTTCTTCCGCGTGCTG
CGTCCGATCTCCGCCGAGGAAACCGAGATCCGTCACGTCGCGCTGGCGATGGACGGCGGCCCCGAGGAAGCGAACCGCGT
GCGCCTGCGGATCCACGAGCATTTCCAGGGCCCGCTCGGTTTCGGCAGCCCCGACGATGCTGAAGCCTGGGAGCGCGTGC
AGCGCGGCGCGCATGCCGGCCCGCACACGCCGATCCTGGTGAACCGCGGCCTGAACCGCGAGACCAGCGCACCGAACGGT
GAGAAGACCGCGCATGCGACCGACGAGACGGGCATGCGCGAGGCATACGCGCAATGGCGCAGGATGATGGAGGCATGA

Upstream 100 bases:

>100_bases
GACGCATGACACCCCCCGCTCGACACTATCGCCAAGCGCCGGCCAGCTCCGGCGATCGACAACCCCGCGTTCCATCGCGA
TCACCACGATACCTCCGACC

Downstream 100 bases:

>100_bases
TGAACCAGCACAACACGCCGGGGTCCGACGACACCTTCGCGCGCGCCCAGCGCTTCATCTGGCGCGAGGCCGAGATGCTC
GACCGCCGCGAGTATCGCGA

Product: Benzoate 1,2-dioxygenase subunit alpha

Products: NAD; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol [C]

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MSETWQSIDTRALRGRAEADRIAPSLYYDPQIFEAELERIFYKTWIWVAHESELPQPGDFRTTTIGRQPVIVVRDRTGTV
NVLQNRCRHRGATVCEEHQGNAKGFTCPYHSWSYALDGALRALPYGDGYEGVCEKGDLPLEKLRVGIYQGLIFASFNQEI
EPLEDFLGGAKPWIDLFMKQGAGYPIKSNGEHKFRFKGNWKIQLENTTDLYHFPVVHKSWMKSIDEQTAEVITRFMTSDK
AFCRSLGNGHSLAVLVPEIVDLDVDDGAPLPERYDALVAQLSARHAPEEVRRIVRSLMGVGFNLNLFPNLALSMAFFRVL
RPISAEETEIRHVALAMDGGPEEANRVRLRIHEHFQGPLGFGSPDDAEAWERVQRGAHAGPHTPILVNRGLNRETSAPNG
EKTAHATDETGMREAYAQWRRMMEA

Sequences:

>Translated_425_residues
MSETWQSIDTRALRGRAEADRIAPSLYYDPQIFEAELERIFYKTWIWVAHESELPQPGDFRTTTIGRQPVIVVRDRTGTV
NVLQNRCRHRGATVCEEHQGNAKGFTCPYHSWSYALDGALRALPYGDGYEGVCEKGDLPLEKLRVGIYQGLIFASFNQEI
EPLEDFLGGAKPWIDLFMKQGAGYPIKSNGEHKFRFKGNWKIQLENTTDLYHFPVVHKSWMKSIDEQTAEVITRFMTSDK
AFCRSLGNGHSLAVLVPEIVDLDVDDGAPLPERYDALVAQLSARHAPEEVRRIVRSLMGVGFNLNLFPNLALSMAFFRVL
RPISAEETEIRHVALAMDGGPEEANRVRLRIHEHFQGPLGFGSPDDAEAWERVQRGAHAGPHTPILVNRGLNRETSAPNG
EKTAHATDETGMREAYAQWRRMMEA
>Mature_424_residues
SETWQSIDTRALRGRAEADRIAPSLYYDPQIFEAELERIFYKTWIWVAHESELPQPGDFRTTTIGRQPVIVVRDRTGTVN
VLQNRCRHRGATVCEEHQGNAKGFTCPYHSWSYALDGALRALPYGDGYEGVCEKGDLPLEKLRVGIYQGLIFASFNQEIE
PLEDFLGGAKPWIDLFMKQGAGYPIKSNGEHKFRFKGNWKIQLENTTDLYHFPVVHKSWMKSIDEQTAEVITRFMTSDKA
FCRSLGNGHSLAVLVPEIVDLDVDDGAPLPERYDALVAQLSARHAPEEVRRIVRSLMGVGFNLNLFPNLALSMAFFRVLR
PISAEETEIRHVALAMDGGPEEANRVRLRIHEHFQGPLGFGSPDDAEAWERVQRGAHAGPHTPILVNRGLNRETSAPNGE
KTAHATDETGMREAYAQWRRMMEA

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=431, Percent_Identity=29.0023201856148, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1788103, Length=210, Percent_Identity=30.4761904761905, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017639
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: 1.14.1.- [C]

Molecular weight: Translated: 48030; Mature: 47899

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETWQSIDTRALRGRAEADRIAPSLYYDPQIFEAELERIFYKTWIWVAHESELPQPGDF
CCCHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCC
RTTTIGRQPVIVVRDRTGTVNVLQNRCRHRGATVCEEHQGNAKGFTCPYHSWSYALDGAL
EEEECCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHCCCCCCCEECCCCCCHHHHHHHH
RALPYGDGYEGVCEKGDLPLEKLRVGIYQGLIFASFNQEIEPLEDFLGGAKPWIDLFMKQ
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHH
GAGYPIKSNGEHKFRFKGNWKIQLENTTDLYHFPVVHKSWMKSIDEQTAEVITRFMTSDK
CCCCCCCCCCCEEEEEECCEEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCH
AFCRSLGNGHSLAVLVPEIVDLDVDDGAPLPERYDALVAQLSARHAPEEVRRIVRSLMGV
HHHHHCCCCCEEEEEECHHHEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
GFNLNLFPNLALSMAFFRVLRPISAEETEIRHVALAMDGGPEEANRVRLRIHEHFQGPLG
CCCEECCHHHHHHHHHHHHHCCCCCCCHHEEEEEEEECCCCCCCCCEEEEEEHHCCCCCC
FGSPDDAEAWERVQRGAHAGPHTPILVNRGLNRETSAPNGEKTAHATDETGMREAYAQWR
CCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RMMEA
HHHCC
>Mature Secondary Structure 
SETWQSIDTRALRGRAEADRIAPSLYYDPQIFEAELERIFYKTWIWVAHESELPQPGDF
CCHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCC
RTTTIGRQPVIVVRDRTGTVNVLQNRCRHRGATVCEEHQGNAKGFTCPYHSWSYALDGAL
EEEECCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHCCCCCCCEECCCCCCHHHHHHHH
RALPYGDGYEGVCEKGDLPLEKLRVGIYQGLIFASFNQEIEPLEDFLGGAKPWIDLFMKQ
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHH
GAGYPIKSNGEHKFRFKGNWKIQLENTTDLYHFPVVHKSWMKSIDEQTAEVITRFMTSDK
CCCCCCCCCCCEEEEEECCEEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCH
AFCRSLGNGHSLAVLVPEIVDLDVDDGAPLPERYDALVAQLSARHAPEEVRRIVRSLMGV
HHHHHCCCCCEEEEEECHHHEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
GFNLNLFPNLALSMAFFRVLRPISAEETEIRHVALAMDGGPEEANRVRLRIHEHFQGPLG
CCCEECCHHHHHHHHHHHHHCCCCCCCHHEEEEEEEECCCCCCCCCEEEEEEHHCCCCCC
FGSPDDAEAWERVQRGAHAGPHTPILVNRGLNRETSAPNGEKTAHATDETGMREAYAQWR
CCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RMMEA
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2Fe-2S. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; Proton; O2; 3-phenylpropionate [C]

Specific reaction: 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD 3-phenylpropionate + NADH + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1740120; 1938949 [H]