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The map label for this gene is mnmC [H]

Identifier: 237814393

GI number: 237814393

Start: 4014962

End: 4016944

Strand: Reverse

Name: mnmC [H]

Synonym: GBP346_A4191

Alternate gene names: 237814393

Gene position: 4016944-4014962 (Counterclockwise)

Preceding gene: 237814396

Following gene: 237814392

Centisome position: 98.01

GC content: 71.71

Gene sequence:

>1983_bases
ATGACCGATCGAATCGTTCCGGCGACGCTCGTCTTCCGCGAGGACGGCACCGTCGTCTCGCCGCTCTACGGCGACATCTA
CCACAGCGCGGCCGGCGCGCTCGCCCAGGCCGACCACGTGTTCATCCGCGGCAACGGCCTGCCGGAACGTTGGCGGCACG
AGCGAGCTTTCACGATTATCGAGACAGGATTCGGCACGGGCTGCAACTTTCTCGCGACATGGGCTGCATGGCGTGCGGAT
CCGTCGCACTGCGAGCGGCTTCACTTCGTCTCGGTCGAAAAGCATCCGTTCGCGCGCGAAGATCTGCGGCGCGCGGCCGC
GCATATCGTTGCATATACAACTATTACAACTATTACGCCGATTGCGCCGCTCGTCGACGAGCTCGCGAACGCATGGCCCG
CGCTGACGCCGGGCGTGCATCGTCTCGAATTCGACGACGGGCGCGTCACGCTCACGCTCGTGTTCGGCGACGCGCTCGAC
GTGCTGCCGAATCTCGCGCTGCGCGCGCACGCGTTCTATCTCGACGGCTTCGCGCCGTCGAAGAACGCGGATTTGTGGTC
GCCCGCGATCTTCAAATCGCTCGCGAAGCTTGCCGACGAACGCGCGACGTTCGCGACCTACACGAGCTCCGGCGCCGTCA
AGCGCGCGCTCGACGAAGCGGGTTTCGCGTACCGAAAAGTCGACGGCTTCGCCGGCAAGCGCGCCATGCTCGTCGGCGAA
TTCGCGCCGCGCTGGCGCGTGCGCCGCCATGAGCCGCCGCGTGCGTTCAGCACGGATAGACGCGACGCGATCGTGATCGG
CGCGGGCCTCGCCGGCTGCGCGGTCGTCGAGCGGCTCGCCGCGCGCGGCTGGCACGTGACGCTGATCGAGCGGCGCGAGC
GGATCGCGAGCGAGGCGTCGGGCAACCCCGCCGGCGTATTCCATCCGATGATCGCGCGCGACGACAACCTCGCGGCCCGC
CTGTCGCGCGCCGGCTTCCTGCACGCGCTGAATCGCTGGCGCGCGCTCGAGCGCGCCGGGCATGCGTTCTCGCGCAGCAC
ACATGGCCTCGTTCAGCTTGCCACGTCGGACGATGAATTCGAGCGGATGCGCGAGAGCATCGACGCGCTCGGCGTGCCGG
CCGAGCTCGCCTCGGCGCTGTCGCGCGACGACGCGAGAGCGCTGTTGCGAACCGACGTCGCGCACGGCGGCTGGCTGTTT
GCGCAGGGCGGCTCGATCAGCCCCGCCGCGCTCGCAGCCGCTCAGTGCGCGGCCGCGGGCGACAGGCTTTCGCGCATCGT
CGGCGTCGAGATCGCGCGGCTCGAACGCGGCGGCGACGGCCGCTGGCGCGCGCTCGACGCATCGGGCGCGACGATTGCGC
AGGCGAGCGTCGTCGTCGTCGCGAACGCGGCCGATGCGGCGCGCATCGCGGGCCTGCGGCATGCGCCGACGCAACGCGTG
CGCGGCCAGTTGACGCTGCTGCCGCCGGGCAGCGCGCCCGCCGTGCCGCTGCCCGTGATCGGCGACGGCTACGTCGTGCC
GCTCGCGAACGGCGTCACGCTGACGGGCGCAACCTATGAACCGGACGATACGGATGCGACACCGCGCGAAGCCGGCCATC
GCGAGAACCTCGAGCGGCTCGAACGGCTGCTGCCGGCGTTTTCCGCGAACGCGCTCGATGCGGGCGCGCTCGCGGGCCGC
GTCGGCTTTCGCTGCGTCGCGAGCGACCGGCTGCCGCTCGTCGGCGAGCTCGGCGACGAGGCGGCGGCCGCGCGCGAGGC
GGCGGCGCTGACGGGCGCACGGCTGCGCGACGTGCCGCGCGCGACGGGCCTCTACGGCGCCTTCGGCTACGGCTCGCGCG
GGCTCGTCTGGGCAGCGCTCGGCGCCGAGCTGATCGCCGCGCAGATCGACGGCGAGCCGTGGCCGCTCGAGCGCGAACTC
GCCGAAGCGATCGACCCGGCGCGCTTCCTCGTTCGCGCGCTGCGGCACGGCCGCGTCGCCTGA

Upstream 100 bases:

>100_bases
CGAAGCCCGCGCGCGGTTTCGTTGGATTTTGCCAACGCCAGGCGGGACGGGGCTCTCGCGGCGGTTCGCCGCATTTTCGC
CGCCTTCATTTTTGTTTCGC

Downstream 100 bases:

>100_bases
ACCGCCCGCAGCCGGCGTACGCTCGCCATCCGGGCAATCGCACGGCGCACCGCGACGGCGCTCGGCGATACGCGGAAGCG
ACATCCTCACAGACACGCCG

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 660; Mature: 659

Protein sequence:

>660_residues
MTDRIVPATLVFREDGTVVSPLYGDIYHSAAGALAQADHVFIRGNGLPERWRHERAFTIIETGFGTGCNFLATWAAWRAD
PSHCERLHFVSVEKHPFAREDLRRAAAHIVAYTTITTITPIAPLVDELANAWPALTPGVHRLEFDDGRVTLTLVFGDALD
VLPNLALRAHAFYLDGFAPSKNADLWSPAIFKSLAKLADERATFATYTSSGAVKRALDEAGFAYRKVDGFAGKRAMLVGE
FAPRWRVRRHEPPRAFSTDRRDAIVIGAGLAGCAVVERLAARGWHVTLIERRERIASEASGNPAGVFHPMIARDDNLAAR
LSRAGFLHALNRWRALERAGHAFSRSTHGLVQLATSDDEFERMRESIDALGVPAELASALSRDDARALLRTDVAHGGWLF
AQGGSISPAALAAAQCAAAGDRLSRIVGVEIARLERGGDGRWRALDASGATIAQASVVVVANAADAARIAGLRHAPTQRV
RGQLTLLPPGSAPAVPLPVIGDGYVVPLANGVTLTGATYEPDDTDATPREAGHRENLERLERLLPAFSANALDAGALAGR
VGFRCVASDRLPLVGELGDEAAAAREAAALTGARLRDVPRATGLYGAFGYGSRGLVWAALGAELIAAQIDGEPWPLEREL
AEAIDPARFLVRALRHGRVA

Sequences:

>Translated_660_residues
MTDRIVPATLVFREDGTVVSPLYGDIYHSAAGALAQADHVFIRGNGLPERWRHERAFTIIETGFGTGCNFLATWAAWRAD
PSHCERLHFVSVEKHPFAREDLRRAAAHIVAYTTITTITPIAPLVDELANAWPALTPGVHRLEFDDGRVTLTLVFGDALD
VLPNLALRAHAFYLDGFAPSKNADLWSPAIFKSLAKLADERATFATYTSSGAVKRALDEAGFAYRKVDGFAGKRAMLVGE
FAPRWRVRRHEPPRAFSTDRRDAIVIGAGLAGCAVVERLAARGWHVTLIERRERIASEASGNPAGVFHPMIARDDNLAAR
LSRAGFLHALNRWRALERAGHAFSRSTHGLVQLATSDDEFERMRESIDALGVPAELASALSRDDARALLRTDVAHGGWLF
AQGGSISPAALAAAQCAAAGDRLSRIVGVEIARLERGGDGRWRALDASGATIAQASVVVVANAADAARIAGLRHAPTQRV
RGQLTLLPPGSAPAVPLPVIGDGYVVPLANGVTLTGATYEPDDTDATPREAGHRENLERLERLLPAFSANALDAGALAGR
VGFRCVASDRLPLVGELGDEAAAAREAAALTGARLRDVPRATGLYGAFGYGSRGLVWAALGAELIAAQIDGEPWPLEREL
AEAIDPARFLVRALRHGRVA
>Mature_659_residues
TDRIVPATLVFREDGTVVSPLYGDIYHSAAGALAQADHVFIRGNGLPERWRHERAFTIIETGFGTGCNFLATWAAWRADP
SHCERLHFVSVEKHPFAREDLRRAAAHIVAYTTITTITPIAPLVDELANAWPALTPGVHRLEFDDGRVTLTLVFGDALDV
LPNLALRAHAFYLDGFAPSKNADLWSPAIFKSLAKLADERATFATYTSSGAVKRALDEAGFAYRKVDGFAGKRAMLVGEF
APRWRVRRHEPPRAFSTDRRDAIVIGAGLAGCAVVERLAARGWHVTLIERRERIASEASGNPAGVFHPMIARDDNLAARL
SRAGFLHALNRWRALERAGHAFSRSTHGLVQLATSDDEFERMRESIDALGVPAELASALSRDDARALLRTDVAHGGWLFA
QGGSISPAALAAAQCAAAGDRLSRIVGVEIARLERGGDGRWRALDASGATIAQASVVVVANAADAARIAGLRHAPTQRVR
GQLTLLPPGSAPAVPLPVIGDGYVVPLANGVTLTGATYEPDDTDATPREAGHRENLERLERLLPAFSANALDAGALAGRV
GFRCVASDRLPLVGELGDEAAAAREAAALTGARLRDVPRATGLYGAFGYGSRGLVWAALGAELIAAQIDGEPWPLERELA
EAIDPARFLVRALRHGRVA

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=680, Percent_Identity=33.6764705882353, Blast_Score=302, Evalue=4e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR016040
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 70707; Mature: 70576

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRIVPATLVFREDGTVVSPLYGDIYHSAAGALAQADHVFIRGNGLPERWRHERAFTII
CCCCEEEEEEEEECCCCEECCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCEEEEE
ETGFGTGCNFLATWAAWRADPSHCERLHFVSVEKHPFAREDLRRAAAHIVAYTTITTITP
ECCCCCCCHHHHHHHHHCCCHHHHCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
IAPLVDELANAWPALTPGVHRLEFDDGRVTLTLVFGDALDVLPNLALRAHAFYLDGFAPS
HHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEECCHHHHHHHHHHHHEEEEEECCCCC
KNADLWSPAIFKSLAKLADERATFATYTSSGAVKRALDEAGFAYRKVDGFAGKRAMLVGE
CCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCEEECCCCCCCCEEEEEC
FAPRWRVRRHEPPRAFSTDRRDAIVIGAGLAGCAVVERLAARGWHVTLIERRERIASEAS
CCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCC
GNPAGVFHPMIARDDNLAARLSRAGFLHALNRWRALERAGHAFSRSTHGLVQLATSDDEF
CCCCCEECHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCEEEEECCCHHH
ERMRESIDALGVPAELASALSRDDARALLRTDVAHGGWLFAQGGSISPAALAAAQCAAAG
HHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHH
DRLSRIVGVEIARLERGGDGRWRALDASGATIAQASVVVVANAADAARIAGLRHAPTQRV
HHHHHHHHHEEEEEECCCCCCEEEEECCCCEEEEEEEEEEECCCCHHHHHCCCCCCCHHH
RGQLTLLPPGSAPAVPLPVIGDGYVVPLANGVTLTGATYEPDDTDATPREAGHRENLERL
CCEEEEECCCCCCCCCCEECCCCEEEEECCCEEEECCCCCCCCCCCCCHHCCCHHHHHHH
ERLLPAFSANALDAGALAGRVGFRCVASDRLPLVGELGDEAAAAREAAALTGARLRDVPR
HHHHHHHCCCCCCHHHHHHHHCEEEEECCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCCC
ATGLYGAFGYGSRGLVWAALGAELIAAQIDGEPWPLERELAEAIDPARFLVRALRHGRVA
CCCCCEECCCCCCCHHHHHHHHHHEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TDRIVPATLVFREDGTVVSPLYGDIYHSAAGALAQADHVFIRGNGLPERWRHERAFTII
CCCEEEEEEEEECCCCEECCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCEEEEE
ETGFGTGCNFLATWAAWRADPSHCERLHFVSVEKHPFAREDLRRAAAHIVAYTTITTITP
ECCCCCCCHHHHHHHHHCCCHHHHCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
IAPLVDELANAWPALTPGVHRLEFDDGRVTLTLVFGDALDVLPNLALRAHAFYLDGFAPS
HHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEECCHHHHHHHHHHHHEEEEEECCCCC
KNADLWSPAIFKSLAKLADERATFATYTSSGAVKRALDEAGFAYRKVDGFAGKRAMLVGE
CCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCEEECCCCCCCCEEEEEC
FAPRWRVRRHEPPRAFSTDRRDAIVIGAGLAGCAVVERLAARGWHVTLIERRERIASEAS
CCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCC
GNPAGVFHPMIARDDNLAARLSRAGFLHALNRWRALERAGHAFSRSTHGLVQLATSDDEF
CCCCCEECHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCEEEEECCCHHH
ERMRESIDALGVPAELASALSRDDARALLRTDVAHGGWLFAQGGSISPAALAAAQCAAAG
HHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHH
DRLSRIVGVEIARLERGGDGRWRALDASGATIAQASVVVVANAADAARIAGLRHAPTQRV
HHHHHHHHHEEEEEECCCCCCEEEEECCCCEEEEEEEEEEECCCCHHHHHCCCCCCCHHH
RGQLTLLPPGSAPAVPLPVIGDGYVVPLANGVTLTGATYEPDDTDATPREAGHRENLERL
CCEEEEECCCCCCCCCCEECCCCEEEEECCCEEEECCCCCCCCCCCCCHHCCCHHHHHHH
ERLLPAFSANALDAGALAGRVGFRCVASDRLPLVGELGDEAAAAREAAALTGARLRDVPR
HHHHHHHCCCCCCHHHHHHHHCEEEEECCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCCC
ATGLYGAFGYGSRGLVWAALGAELIAAQIDGEPWPLERELAEAIDPARFLVRALRHGRVA
CCCCCEECCCCCCCHHHHHHHHHHEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA