Click here to switch to the map view.

The map label for this gene is cls [H]

Identifier: 237812824

GI number: 237812824

Start: 2514959

End: 2516398

Strand: Direct

Name: cls [H]

Synonym: GBP346_A2578

Alternate gene names: 237812824

Gene position: 2514959-2516398 (Clockwise)

Preceding gene: 237812823

Following gene: 237812826

Centisome position: 61.36

GC content: 68.89

Gene sequence:

>1440_bases
ATGACCCTCGACTGGCTCCATCTCGGCCCCCTCATCGGCCTCGCGCACGTGCTCGGCGTGATCGCGGCCTGCCATGCGAT
CCTGAACACGCGCACGTCGCAAGGCGCGATCGCGTGGGCGGTGTCGTTGACGGCGATGCCGTACCTGACGCTGATCCCGT
ATCTGTTCCTCGGCCGCAGCAAGTTCTCCGGCTATGTCGACGCGCGCCGCCACGAGACCGCGGCGCTGCGCACCCGCACG
CATCCCGCGCCGTGGGACGCCGAAGGCTCGACGCTCGGCGCGCCGAAGGAAGCGCTCGGCGCCGCGCGCGTACGCGCGCT
CACGCGCCTGGTCGGCATGCCGTTCGTCGCCGGCAACGCGGTGCGCACGCTCGTCAACGGCGACGCGACGTTCTCCGCGA
TCCTCGCCGCGATCGACGCCGCGCGCAGCTACGTGATCGTCCAGTTCTTCATCGTCCGCGACGACGCGCTCGGCGCGATG
CTGCGCGACACGCTGATCGCGCGCGCGAAGGCGGGCGTGCGCTGCTATCTGCTGTACGACAGCATCGGCAGCTTCGATCT
GCCGTCGAGCTACGTGCACGCGCTGCGCGAAGGCGGCGTCGAAGTGCATCCGTTCGCGACCAACAAGCAGTTCGTCAACC
GCTTCCAGCTCAATTTCCGCAATCACCGGAAGATCGTCGTCGTCGACGGCGAGCGCGCGTTCGTCGGCGGGCACAACGTC
GGCGTCGAATATCTCGGCGGCAACCCGCGCCTGTCGCCGTGGCGCGACACGCACATCGAAGTGCGCGGCCCGGCGGCCGC
GAACATCCAGTACGTGTTCGCCGAGGACTGGCACTGGGCAACCCAGTCGCTCCCCCCGCAGGCACCGCCGCCCGCGCCGG
TTCCCGACGCCGACATGCACTGCCTCGTCGTGCCGATGGGCCCCGCCGACAAGCAGGAGACAGGCTCGCTCTTCTTCGCG
GAGGCGATCAACGCCGCGCGCGAGCGCGTGTGGATCACGACGCCCTACCTCGTGCCCGACGAAGCGGTGATTTCCGCGCT
GAAGCTCGCGGTGATGCGCGGCGTGGACGTGCGGGTGATGATTCCGAGCCGGCGCGATCACTATGTCGTTTTCGAAGCGT
CGAAGCTCTATGCGCGCGATCTCGTCGACGCGGGCGTGCGAATCTTCCGCTACCGCCCCGGCTTCCTGCACCAGAAGGTC
GTGCTGATCGACCGGGCGGCCGCCGCCGTCGGCAGCGCGAACCTCGACAACCGTTCGTTCCGGCTGAATTTCGAGATCAT
GGTGCTGACCGTCGACGCGGGCTTCGCCGCCGAAGTCGAAGCGATGCTCGCGCGCGACTTCGATCAAGCGTTCGAGGTCG
ACCTGAGCGAGTACCGGCGCTCGCCCGCGTGGCGGCGCGTGGCGATGCACGTCGCACGGCTGTTCGCGCCGATCCTCTGA

Upstream 100 bases:

>100_bases
TCATGCGGGGTGGCCGCTTGCGCGCACGCTTCGGTAAGATGCGCGTAAGCGGCGCCCGGTCGCGGCGCCGCGTGGCCTCC
TTCTGGTATCCGACGCGCTC

Downstream 100 bases:

>100_bases
CGACGCGCGGCGCGCGAGCGGGCGCCGGGCGGCCCGCCCGAACGGCCCCGACGGCCCCGGCGGCCCGCGCAGCCCGCATG
CCCGATCCGGGCCGCGCGGC

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTLDWLHLGPLIGLAHVLGVIAACHAILNTRTSQGAIAWAVSLTAMPYLTLIPYLFLGRSKFSGYVDARRHETAALRTRT
HPAPWDAEGSTLGAPKEALGAARVRALTRLVGMPFVAGNAVRTLVNGDATFSAILAAIDAARSYVIVQFFIVRDDALGAM
LRDTLIARAKAGVRCYLLYDSIGSFDLPSSYVHALREGGVEVHPFATNKQFVNRFQLNFRNHRKIVVVDGERAFVGGHNV
GVEYLGGNPRLSPWRDTHIEVRGPAAANIQYVFAEDWHWATQSLPPQAPPPAPVPDADMHCLVVPMGPADKQETGSLFFA
EAINAARERVWITTPYLVPDEAVISALKLAVMRGVDVRVMIPSRRDHYVVFEASKLYARDLVDAGVRIFRYRPGFLHQKV
VLIDRAAAAVGSANLDNRSFRLNFEIMVLTVDAGFAAEVEAMLARDFDQAFEVDLSEYRRSPAWRRVAMHVARLFAPIL

Sequences:

>Translated_479_residues
MTLDWLHLGPLIGLAHVLGVIAACHAILNTRTSQGAIAWAVSLTAMPYLTLIPYLFLGRSKFSGYVDARRHETAALRTRT
HPAPWDAEGSTLGAPKEALGAARVRALTRLVGMPFVAGNAVRTLVNGDATFSAILAAIDAARSYVIVQFFIVRDDALGAM
LRDTLIARAKAGVRCYLLYDSIGSFDLPSSYVHALREGGVEVHPFATNKQFVNRFQLNFRNHRKIVVVDGERAFVGGHNV
GVEYLGGNPRLSPWRDTHIEVRGPAAANIQYVFAEDWHWATQSLPPQAPPPAPVPDADMHCLVVPMGPADKQETGSLFFA
EAINAARERVWITTPYLVPDEAVISALKLAVMRGVDVRVMIPSRRDHYVVFEASKLYARDLVDAGVRIFRYRPGFLHQKV
VLIDRAAAAVGSANLDNRSFRLNFEIMVLTVDAGFAAEVEAMLARDFDQAFEVDLSEYRRSPAWRRVAMHVARLFAPIL
>Mature_478_residues
TLDWLHLGPLIGLAHVLGVIAACHAILNTRTSQGAIAWAVSLTAMPYLTLIPYLFLGRSKFSGYVDARRHETAALRTRTH
PAPWDAEGSTLGAPKEALGAARVRALTRLVGMPFVAGNAVRTLVNGDATFSAILAAIDAARSYVIVQFFIVRDDALGAML
RDTLIARAKAGVRCYLLYDSIGSFDLPSSYVHALREGGVEVHPFATNKQFVNRFQLNFRNHRKIVVVDGERAFVGGHNVG
VEYLGGNPRLSPWRDTHIEVRGPAAANIQYVFAEDWHWATQSLPPQAPPPAPVPDADMHCLVVPMGPADKQETGSLFFAE
AINAARERVWITTPYLVPDEAVISALKLAVMRGVDVRVMIPSRRDHYVVFEASKLYARDLVDAGVRIFRYRPGFLHQKVV
LIDRAAAAVGSANLDNRSFRLNFEIMVLTVDAGFAAEVEAMLARDFDQAFEVDLSEYRRSPAWRRVAMHVARLFAPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=467, Percent_Identity=29.1220556745182, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1787007, Length=354, Percent_Identity=29.9435028248588, Blast_Score=128, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 52815; Mature: 52684

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDWLHLGPLIGLAHVLGVIAACHAILNTRTSQGAIAWAVSLTAMPYLTLIPYLFLGRS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCC
KFSGYVDARRHETAALRTRTHPAPWDAEGSTLGAPKEALGAARVRALTRLVGMPFVAGNA
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCH
VRTLVNGDATFSAILAAIDAARSYVIVQFFIVRDDALGAMLRDTLIARAKAGVRCYLLYD
HHEEECCCHHHHHHHHHHHHHCCEEEEEEEEEECCHHHHHHHHHHHHHHCCCEEEEEEEE
SIGSFDLPSSYVHALREGGVEVHPFATNKQFVNRFQLNFRNHRKIVVVDGERAFVGGHNV
CCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCCCEEEEECCCEEEECCCCC
GVEYLGGNPRLSPWRDTHIEVRGPAAANIQYVFAEDWHWATQSLPPQAPPPAPVPDADMH
CEEEECCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCE
CLVVPMGPADKQETGSLFFAEAINAARERVWITTPYLVPDEAVISALKLAVMRGVDVRVM
EEEEECCCCCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEE
IPSRRDHYVVFEASKLYARDLVDAGVRIFRYRPGFLHQKVVLIDRAAAAVGSANLDNRSF
ECCCCCCEEEEECHHHHHHHHHHCCCEEEEECCCCHHCEEEEEEHHHHHHCCCCCCCCEE
RLNFEIMVLTVDAGFAAEVEAMLARDFDQAFEVDLSEYRRSPAWRRVAMHVARLFAPIL
EEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLDWLHLGPLIGLAHVLGVIAACHAILNTRTSQGAIAWAVSLTAMPYLTLIPYLFLGRS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCC
KFSGYVDARRHETAALRTRTHPAPWDAEGSTLGAPKEALGAARVRALTRLVGMPFVAGNA
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCH
VRTLVNGDATFSAILAAIDAARSYVIVQFFIVRDDALGAMLRDTLIARAKAGVRCYLLYD
HHEEECCCHHHHHHHHHHHHHCCEEEEEEEEEECCHHHHHHHHHHHHHHCCCEEEEEEEE
SIGSFDLPSSYVHALREGGVEVHPFATNKQFVNRFQLNFRNHRKIVVVDGERAFVGGHNV
CCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCCCEEEEECCCEEEECCCCC
GVEYLGGNPRLSPWRDTHIEVRGPAAANIQYVFAEDWHWATQSLPPQAPPPAPVPDADMH
CEEEECCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCE
CLVVPMGPADKQETGSLFFAEAINAARERVWITTPYLVPDEAVISALKLAVMRGVDVRVM
EEEEECCCCCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEE
IPSRRDHYVVFEASKLYARDLVDAGVRIFRYRPGFLHQKVVLIDRAAAAVGSANLDNRSF
ECCCCCCEEEEECHHHHHHHHHHCCCEEEEECCCCHHCEEEEEEHHHHHHCCCCCCCCEE
RLNFEIMVLTVDAGFAAEVEAMLARDFDQAFEVDLSEYRRSPAWRRVAMHVARLFAPIL
EEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA