Click here to switch to the map view.

The map label for this gene is gidA [H]

Identifier: 23502909

GI number: 23502909

Start: 1984986

End: 1986896

Strand: Reverse

Name: gidA [H]

Synonym: BR2061

Alternate gene names: 23502909

Gene position: 1986896-1984986 (Counterclockwise)

Preceding gene: 23502910

Following gene: 23502908

Centisome position: 94.26

GC content: 56.72

Gene sequence:

>1911_bases
ATGAGTTCGGCCGAAGCTCTTGCCTTTGATGTCATTGTAATTGGTGGTGGCCATGCGGGCTGCGAAGCCGCTTCCGCTGC
CGCGCGTGCGGGCGCGCGCACGGCGCTTGTTACGCATCGTTTTGATACGATCGGGGTCATGTCCTGTAACCCAGCGATTG
GCGGGCTGGGAAAAGGCCATCTTGTTCGTGAAATCGATGCCCTTGATGGCCTGATGGGGCGCGTGGCTGATAGAGCAGGC
ATCCAGTTTCGCTTGCTCAATCGGCGCAAGGGCCCGGCGGTGCGAGGGCCTCGTACGCAGGCTGATCGCAAGCTTTATCG
TCTGGCCATGCAGCAAATGATCACGGAACAGGAGAACCTCACCGTTGTTGAGGGCGGTGCTGCTGATCTTGTCTGCGATG
GCGAACGAATTTCCGGTGTCACTCTTGCGGATGGTCGCGTTTTGAAATGTGGTGCCGTGGTTCTCACAACAGGCACGTTT
CTTAACGGCCTTATTCATATAGGCGAAAAGAGGTTTCCAGCTGGCCGTATGGGGGAAAAGCCCGCCCTGGGTCTTTCCGA
ACGCTTGCTTTCCTTTGGCTTCACGCTTGGGCGTCTTAAAACCGGCACACCGCCGCGTCTTGATGGTCGCACAATCGACT
GGCAGAGTCTCGATATGCAATCGGCAGATGAGGAGCCTGTTCCGTTCTCGCTTATGACGGATCGCATCACCACGCCGCAG
ATCGAGTGTGGTATTACCCGCACCACGCCAGAAACGCACGATATCATCCGGGCGAATCTGCACCGCTCAGCCATGTATTC
CGGCAGTATCGAAGGGATCGGGCCGCGCTATTGTCCGTCTGTCGAGGATAAGATCGTCAAGTTCGGCGACCGTGATGGTC
ACCAGATATTCCTGGAGCCGGAAGGGCTTGATGACGATACGGTTTACCCCAACGGCATCTCGACCTCGCTGCCGGAAGAT
GTGCAGCTTGAAATTTTGAAGACCATACCCGGTCTGGAAAAGGCAGTGCTGCTCCAGCCGGGCTATGCCATTGAATATGA
CTTTATCGATCCGCGCGAGTTGAAGCGCAGCCTTGAAACGCGCAAGGTCTGCGGTCTTTTTCTGGCCGGGCAGATCAATG
GCACGACAGGATATGAGGAGGCGGGCGCTCAAGGGTTGCTGGCCGGGCTTAATGCGGCGCGCCGTGCGGCAGGGAGCGAG
CCTGTCATACTCCAGCGAACGGAAGCCTATATTGGTGTGATGGTGGATGATCTGACGTCTCGCGGCGTGAGCGAACCCTA
TCGCATGTTTACGTCGCGAGCCGAGTTCCGGCTTTCGCTGCGTGCTGATAATGCCGATCAGCGGTTGACCCCCCTTGCTG
ATGAAGTTGGAATCTTGAGCAAAGAGCGTCGTAAACGCTATTTAACGCGTGAGACTGCATTATCTCATGCGCGCATGGTG
ACGCAATCGCTGTCGATTACCCCCAATCTTGCCGGGTATTATGATCTGCGGCTCAATCAGGACGGTGTTCGCCGCTCCGC
CTATGATCTGCTTTCCTATCCCGATATCAATCTGGACCGCCTGATCGCCATCTGGCCGGAACTGGCATCGATCGATCCCG
TCACGCGGGAAGCACTTGAGATTGAAGCCCAATATGCGGTCTATATGGAGCGGCAGCAGAGTGATATTGCCGTCATGGAA
CGGGAAGAGCGGCTGCTTATACCGTCGGGGCTTGATTTTGATGCCATCTCCGGGCTTTCCAATGAATTGAAGCAAAAGCT
GAAGCAGAGGAAGCCGGAAACGATTGCGGAAGCGCAGCGCGTCGATGGGATGACCCCGGCCGCGGTGGCTTTATTGATCG
CGCAGATCAGAAAATTCGGCGGCAGGCAGAAACTGGCTGCCGAAACGCTGGAAGGCAAGGGCGCAGCATGA

Upstream 100 bases:

>100_bases
CGAGTAGAAGGGCGGACATAGTTTTTCCTTCCGTTTGAAGGCCATTCATGTCAAAGAAGCTTCTTTCAGAAACGGATTCG
AATTGCGTCAGGATGAACCC

Downstream 100 bases:

>100_bases
GCGCCGATATCCGCTTTGATAGCCTGAAAACCATCGTGCCGGCTGTTTCACGTGAAACAGCCGATCGCCTGATCGCGTTC
GAGGATCTGTTCCGAAAATG

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 636; Mature: 635

Protein sequence:

>636_residues
MSSAEALAFDVIVIGGGHAGCEAASAAARAGARTALVTHRFDTIGVMSCNPAIGGLGKGHLVREIDALDGLMGRVADRAG
IQFRLLNRRKGPAVRGPRTQADRKLYRLAMQQMITEQENLTVVEGGAADLVCDGERISGVTLADGRVLKCGAVVLTTGTF
LNGLIHIGEKRFPAGRMGEKPALGLSERLLSFGFTLGRLKTGTPPRLDGRTIDWQSLDMQSADEEPVPFSLMTDRITTPQ
IECGITRTTPETHDIIRANLHRSAMYSGSIEGIGPRYCPSVEDKIVKFGDRDGHQIFLEPEGLDDDTVYPNGISTSLPED
VQLEILKTIPGLEKAVLLQPGYAIEYDFIDPRELKRSLETRKVCGLFLAGQINGTTGYEEAGAQGLLAGLNAARRAAGSE
PVILQRTEAYIGVMVDDLTSRGVSEPYRMFTSRAEFRLSLRADNADQRLTPLADEVGILSKERRKRYLTRETALSHARMV
TQSLSITPNLAGYYDLRLNQDGVRRSAYDLLSYPDINLDRLIAIWPELASIDPVTREALEIEAQYAVYMERQQSDIAVME
REERLLIPSGLDFDAISGLSNELKQKLKQRKPETIAEAQRVDGMTPAAVALLIAQIRKFGGRQKLAAETLEGKGAA

Sequences:

>Translated_636_residues
MSSAEALAFDVIVIGGGHAGCEAASAAARAGARTALVTHRFDTIGVMSCNPAIGGLGKGHLVREIDALDGLMGRVADRAG
IQFRLLNRRKGPAVRGPRTQADRKLYRLAMQQMITEQENLTVVEGGAADLVCDGERISGVTLADGRVLKCGAVVLTTGTF
LNGLIHIGEKRFPAGRMGEKPALGLSERLLSFGFTLGRLKTGTPPRLDGRTIDWQSLDMQSADEEPVPFSLMTDRITTPQ
IECGITRTTPETHDIIRANLHRSAMYSGSIEGIGPRYCPSVEDKIVKFGDRDGHQIFLEPEGLDDDTVYPNGISTSLPED
VQLEILKTIPGLEKAVLLQPGYAIEYDFIDPRELKRSLETRKVCGLFLAGQINGTTGYEEAGAQGLLAGLNAARRAAGSE
PVILQRTEAYIGVMVDDLTSRGVSEPYRMFTSRAEFRLSLRADNADQRLTPLADEVGILSKERRKRYLTRETALSHARMV
TQSLSITPNLAGYYDLRLNQDGVRRSAYDLLSYPDINLDRLIAIWPELASIDPVTREALEIEAQYAVYMERQQSDIAVME
REERLLIPSGLDFDAISGLSNELKQKLKQRKPETIAEAQRVDGMTPAAVALLIAQIRKFGGRQKLAAETLEGKGAA
>Mature_635_residues
SSAEALAFDVIVIGGGHAGCEAASAAARAGARTALVTHRFDTIGVMSCNPAIGGLGKGHLVREIDALDGLMGRVADRAGI
QFRLLNRRKGPAVRGPRTQADRKLYRLAMQQMITEQENLTVVEGGAADLVCDGERISGVTLADGRVLKCGAVVLTTGTFL
NGLIHIGEKRFPAGRMGEKPALGLSERLLSFGFTLGRLKTGTPPRLDGRTIDWQSLDMQSADEEPVPFSLMTDRITTPQI
ECGITRTTPETHDIIRANLHRSAMYSGSIEGIGPRYCPSVEDKIVKFGDRDGHQIFLEPEGLDDDTVYPNGISTSLPEDV
QLEILKTIPGLEKAVLLQPGYAIEYDFIDPRELKRSLETRKVCGLFLAGQINGTTGYEEAGAQGLLAGLNAARRAAGSEP
VILQRTEAYIGVMVDDLTSRGVSEPYRMFTSRAEFRLSLRADNADQRLTPLADEVGILSKERRKRYLTRETALSHARMVT
QSLSITPNLAGYYDLRLNQDGVRRSAYDLLSYPDINLDRLIAIWPELASIDPVTREALEIEAQYAVYMERQQSDIAVMER
EERLLIPSGLDFDAISGLSNELKQKLKQRKPETIAEAQRVDGMTPAAVALLIAQIRKFGGRQKLAAETLEGKGAA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=644, Percent_Identity=45.4968944099379, Blast_Score=541, Evalue=1e-154,
Organism=Homo sapiens, GI19882217, Length=669, Percent_Identity=43.796711509716, Blast_Score=527, Evalue=1e-149,
Organism=Homo sapiens, GI183227703, Length=684, Percent_Identity=42.8362573099415, Blast_Score=521, Evalue=1e-148,
Organism=Escherichia coli, GI2367273, Length=618, Percent_Identity=51.7799352750809, Blast_Score=624, Evalue=1e-180,
Organism=Caenorhabditis elegans, GI17534255, Length=620, Percent_Identity=44.5161290322581, Blast_Score=515, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6321202, Length=627, Percent_Identity=47.5279106858054, Blast_Score=576, Evalue=1e-165,
Organism=Drosophila melanogaster, GI24658174, Length=637, Percent_Identity=48.9795918367347, Blast_Score=566, Evalue=1e-161,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 69432; Mature: 69301

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAEALAFDVIVIGGGHAGCEAASAAARAGARTALVTHRFDTIGVMSCNPAIGGLGKGH
CCCCCEEEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCCCCH
LVREIDALDGLMGRVADRAGIQFRLLNRRKGPAVRGPRTQADRKLYRLAMQQMITEQENL
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCE
TVVEGGAADLVCDGERISGVTLADGRVLKCGAVVLTTGTFLNGLIHIGEKRFPAGRMGEK
EEEECCCCEEEECCCCCCCEEECCCCEEECCEEEEEHHHHHHHHHHHCCCCCCCCCCCCC
PALGLSERLLSFGFTLGRLKTGTPPRLDGRTIDWQSLDMQSADEEPVPFSLMTDRITTPQ
CCCCHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCC
IECGITRTTPETHDIIRANLHRSAMYSGSIEGIGPRYCPSVEDKIVKFGDRDGHQIFLEP
EEECEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEC
EGLDDDTVYPNGISTSLPEDVQLEILKTIPGLEKAVLLQPGYAIEYDFIDPRELKRSLET
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCEEEECCCCHHHHHHHHHH
RKVCGLFLAGQINGTTGYEEAGAQGLLAGLNAARRAAGSEPVILQRTEAYIGVMVDDLTS
HHHHHEEEEEEECCCCCCHHHCCHHHHHHHHHHHHHCCCCCEEEEECCHHEEHEEHHHHH
RGVSEPYRMFTSRAEFRLSLRADNADQRLTPLADEVGILSKERRKRYLTRETALSHARMV
CCCCHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TQSLSITPNLAGYYDLRLNQDGVRRSAYDLLSYPDINLDRLIAIWPELASIDPVTREALE
HHHHCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHCCCHHCCCHHHHHHHH
IEAQYAVYMERQQSDIAVMEREERLLIPSGLDFDAISGLSNELKQKLKQRKPETIAEAQR
HHHHHHEEEHHCCCCHHEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VDGMTPAAVALLIAQIRKFGGRQKLAAETLEGKGAA
HCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
SSAEALAFDVIVIGGGHAGCEAASAAARAGARTALVTHRFDTIGVMSCNPAIGGLGKGH
CCCCEEEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCCCCH
LVREIDALDGLMGRVADRAGIQFRLLNRRKGPAVRGPRTQADRKLYRLAMQQMITEQENL
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCE
TVVEGGAADLVCDGERISGVTLADGRVLKCGAVVLTTGTFLNGLIHIGEKRFPAGRMGEK
EEEECCCCEEEECCCCCCCEEECCCCEEECCEEEEEHHHHHHHHHHHCCCCCCCCCCCCC
PALGLSERLLSFGFTLGRLKTGTPPRLDGRTIDWQSLDMQSADEEPVPFSLMTDRITTPQ
CCCCHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCC
IECGITRTTPETHDIIRANLHRSAMYSGSIEGIGPRYCPSVEDKIVKFGDRDGHQIFLEP
EEECEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEC
EGLDDDTVYPNGISTSLPEDVQLEILKTIPGLEKAVLLQPGYAIEYDFIDPRELKRSLET
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCEEEECCCCHHHHHHHHHH
RKVCGLFLAGQINGTTGYEEAGAQGLLAGLNAARRAAGSEPVILQRTEAYIGVMVDDLTS
HHHHHEEEEEEECCCCCCHHHCCHHHHHHHHHHHHHCCCCCEEEEECCHHEEHEEHHHHH
RGVSEPYRMFTSRAEFRLSLRADNADQRLTPLADEVGILSKERRKRYLTRETALSHARMV
CCCCHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TQSLSITPNLAGYYDLRLNQDGVRRSAYDLLSYPDINLDRLIAIWPELASIDPVTREALE
HHHHCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHCCCHHCCCHHHHHHHH
IEAQYAVYMERQQSDIAVMEREERLLIPSGLDFDAISGLSNELKQKLKQRKPETIAEAQR
HHHHHHEEEHHCCCCHHEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VDGMTPAAVALLIAQIRKFGGRQKLAAETLEGKGAA
HCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA