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The map label for this gene is gidB [H]

Identifier: 23502908

GI number: 23502908

Start: 1984348

End: 1984989

Strand: Reverse

Name: gidB [H]

Synonym: BR2060

Alternate gene names: 23502908

Gene position: 1984989-1984348 (Counterclockwise)

Preceding gene: 23502909

Following gene: 23502907

Centisome position: 94.17

GC content: 54.36

Gene sequence:

>642_bases
ATGAGCGCCGATATCCGCTTTGATAGCCTGAAAACCATCGTGCCGGCTGTTTCACGTGAAACAGCCGATCGCCTGATCGC
GTTCGAGGATCTGTTCCGAAAATGGTCGAAGGCCATCAATCTGGCTTCTCCCTCGACGCTCGCCGATCTATGGAACCGGC
ATATTCTTGATAGTGCGCAGCTTTTTCCTCTCGCAAAAGAAGCCACGCGCTGGCTGGATATCGGTTCCGGTGGCGGATTT
CCGGGGATTGTAACCGCCTGTTTTCTGGCCGAACGTTCTGGCGGCTGCATTGATCTGGTGGAAAGTGCGGGTAAAAAGGC
TGCCTTTTTACGCACGGCTGCCGGGCATCTCTATGTCCCGGCACGCGTTCACTCCGCGCGCATCGAATCCATGTGGGAAA
AAATTGAAACACCTCAGGTGGTTACGGCGCGCGCGCTTGCATCGCTGGGCGATCTTTTCACCCTTGCTGAGCCATGGTTG
AGCGACGGCGCAAAGGCCCTTTTCCAAAAAGGTCGGGATTATCAGCGCGAAATCGACGAAAGCCGTGTCGGTTGGAGTTT
CGATCTGGTAAAACATCCTAGTGCTATCGATCAGGCTTCGGTCATACTCGAAATCAGCAATCTGCGCCGTAAAACCGATT
GA

Upstream 100 bases:

>100_bases
GGATGACCCCGGCCGCGGTGGCTTTATTGATCGCGCAGATCAGAAAATTCGGCGGCAGGCAGAAACTGGCTGCCGAAACG
CTGGAAGGCAAGGGCGCAGC

Downstream 100 bases:

>100_bases
TATTTCGCAAATTGTGTTTTTCGGCAGGGCGAGCAGCAGCAAGGCGACGGTAATGAACAAGATGTCCAGGATCATAACCA
TTGCGAACCAGAAGGGTGGG

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MSADIRFDSLKTIVPAVSRETADRLIAFEDLFRKWSKAINLASPSTLADLWNRHILDSAQLFPLAKEATRWLDIGSGGGF
PGIVTACFLAERSGGCIDLVESAGKKAAFLRTAAGHLYVPARVHSARIESMWEKIETPQVVTARALASLGDLFTLAEPWL
SDGAKALFQKGRDYQREIDESRVGWSFDLVKHPSAIDQASVILEISNLRRKTD

Sequences:

>Translated_213_residues
MSADIRFDSLKTIVPAVSRETADRLIAFEDLFRKWSKAINLASPSTLADLWNRHILDSAQLFPLAKEATRWLDIGSGGGF
PGIVTACFLAERSGGCIDLVESAGKKAAFLRTAAGHLYVPARVHSARIESMWEKIETPQVVTARALASLGDLFTLAEPWL
SDGAKALFQKGRDYQREIDESRVGWSFDLVKHPSAIDQASVILEISNLRRKTD
>Mature_212_residues
SADIRFDSLKTIVPAVSRETADRLIAFEDLFRKWSKAINLASPSTLADLWNRHILDSAQLFPLAKEATRWLDIGSGGGFP
GIVTACFLAERSGGCIDLVESAGKKAAFLRTAAGHLYVPARVHSARIESMWEKIETPQVVTARALASLGDLFTLAEPWLS
DGAKALFQKGRDYQREIDESRVGWSFDLVKHPSAIDQASVILEISNLRRKTD

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=145, Percent_Identity=31.0344827586207, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: =2.1.1.170 [H]

Molecular weight: Translated: 23499; Mature: 23368

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADIRFDSLKTIVPAVSRETADRLIAFEDLFRKWSKAINLASPSTLADLWNRHILDSAQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHH
LFPLAKEATRWLDIGSGGGFPGIVTACFLAERSGGCIDLVESAGKKAAFLRTAAGHLYVP
HHHHHHHHHHHEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHCCCCEEEC
ARVHSARIESMWEKIETPQVVTARALASLGDLFTLAEPWLSDGAKALFQKGRDYQREIDE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SRVGWSFDLVKHPSAIDQASVILEISNLRRKTD
HHCCCEEHHHHCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SADIRFDSLKTIVPAVSRETADRLIAFEDLFRKWSKAINLASPSTLADLWNRHILDSAQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHH
LFPLAKEATRWLDIGSGGGFPGIVTACFLAERSGGCIDLVESAGKKAAFLRTAAGHLYVP
HHHHHHHHHHHEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHCCCCEEEC
ARVHSARIESMWEKIETPQVVTARALASLGDLFTLAEPWLSDGAKALFQKGRDYQREIDE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SRVGWSFDLVKHPSAIDQASVILEISNLRRKTD
HHCCCEEHHHHCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA