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The map label for this gene is afr [H]

Identifier: 23502896

GI number: 23502896

Start: 1973207

End: 1974208

Strand: Reverse

Name: afr [H]

Synonym: BR2048

Alternate gene names: 23502896

Gene position: 1974208-1973207 (Counterclockwise)

Preceding gene: 23502897

Following gene: 23502895

Centisome position: 93.66

GC content: 59.88

Gene sequence:

>1002_bases
ATGGCAAAGTGGGGCCTTATAGGCGCAAGCACCATTGCAAAGGAATGGGTGATCGGCACCATTCGCGCGACGGGTGGCGA
GGTCGTTTCCGTCTACAGCACCAATGCCGCGCGCGGTAAAACCTATGCAGACGAGAACGGCATCGCCCGTTCGGTGACGA
GCCTCGATGCGCTGCTGGCCGACGCGGAAATAGACGCGGTTTATATCTCCACCACCAATGAACTGCATCGCGATCAGGCC
ATTGCCGCGGCACAAGCGGGCAAGCATATCCTGTGCGAAAAGCCGCTGGCGCTGACGATTGGTGACGCTCATGAAATGCT
GAAAGCGGCGCGCGCAGCGGGCGTGGTGGCAGGCACCAATCATCATTTGCGCAATGCGGCAAGCCACCGCGCCATGCGCG
ATGCCGTAGCAGAAGGCCGGATCGGCAAGGTGCTGGGCGCGCGGGTTTTCCATGCGGTTTATTTGCCGCCACACTTACAG
GGCTGGCGCATTGAGCGGCCCGATGCGGGCGGCGGTGTCGTCCTCGATATCACTGTGCATGATGCCGATACGCTGCGTTT
TGTGCTGGGGGAAAATCCGGTCGAGGCGGTCGCTTTCAGTCAACAGGGCGGCTTAAGTGGAGAAGGGCTGGAAGATGGCG
TGATGGGCGTTTTGCGTTTTCCATCCGGGGCGATTGCCCAGTTTCACGATGCTTTCACCGCCAAATATGCCGAAACCGGT
TTCGGGGTGCATGGCAGCGAAGGTTCGCTCATTGCGCGCAATGTGATGACGCAAAAGCCGGTCGGGACCGTCATCCTGCG
GGACAAGGACGGCGAACACGAATTGCCGCTCGACCACAAAAATCTCTACGAAACCGCCTTGCAGGCTTTTCACGATGCCA
TTGCCGGAAGAGGCCAGCCATCGGCCACATTGGAGGATGGCATCTGGTCGCTCGCCACCGGGCTGGCCGTGCTTGAGGCC
GCAAAAACCGGCAAAGCCACCGCCGTTCATTCAGGACTTTAA

Upstream 100 bases:

>100_bases
ATTCGGAACGGGTGGAGGCGGAGGCGCAGCGTCTGTCGGTGCGTCTTGCAGTGCGTGCAAGTTGCGGCGCGCCGTTCAGA
AAACCGGAGGAGAAATTTGA

Downstream 100 bases:

>100_bases
GACTTATGAGCAAGCATATTTCATTTGCTGAAGCCGCTGCACTTATCCCCGACAATGCGGTTGTTTCGGTTTCCTCGTCC
AGTGGCCTCGGTTGCCCGGA

Product: Gfo/Idh/MocA family oxidoreductase

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MAKWGLIGASTIAKEWVIGTIRATGGEVVSVYSTNAARGKTYADENGIARSVTSLDALLADAEIDAVYISTTNELHRDQA
IAAAQAGKHILCEKPLALTIGDAHEMLKAARAAGVVAGTNHHLRNAASHRAMRDAVAEGRIGKVLGARVFHAVYLPPHLQ
GWRIERPDAGGGVVLDITVHDADTLRFVLGENPVEAVAFSQQGGLSGEGLEDGVMGVLRFPSGAIAQFHDAFTAKYAETG
FGVHGSEGSLIARNVMTQKPVGTVILRDKDGEHELPLDHKNLYETALQAFHDAIAGRGQPSATLEDGIWSLATGLAVLEA
AKTGKATAVHSGL

Sequences:

>Translated_333_residues
MAKWGLIGASTIAKEWVIGTIRATGGEVVSVYSTNAARGKTYADENGIARSVTSLDALLADAEIDAVYISTTNELHRDQA
IAAAQAGKHILCEKPLALTIGDAHEMLKAARAAGVVAGTNHHLRNAASHRAMRDAVAEGRIGKVLGARVFHAVYLPPHLQ
GWRIERPDAGGGVVLDITVHDADTLRFVLGENPVEAVAFSQQGGLSGEGLEDGVMGVLRFPSGAIAQFHDAFTAKYAETG
FGVHGSEGSLIARNVMTQKPVGTVILRDKDGEHELPLDHKNLYETALQAFHDAIAGRGQPSATLEDGIWSLATGLAVLEA
AKTGKATAVHSGL
>Mature_332_residues
AKWGLIGASTIAKEWVIGTIRATGGEVVSVYSTNAARGKTYADENGIARSVTSLDALLADAEIDAVYISTTNELHRDQAI
AAAQAGKHILCEKPLALTIGDAHEMLKAARAAGVVAGTNHHLRNAASHRAMRDAVAEGRIGKVLGARVFHAVYLPPHLQG
WRIERPDAGGGVVLDITVHDADTLRFVLGENPVEAVAFSQQGGLSGEGLEDGVMGVLRFPSGAIAQFHDAFTAKYAETGF
GVHGSEGSLIARNVMTQKPVGTVILRDKDGEHELPLDHKNLYETALQAFHDAIAGRGQPSATLEDGIWSLATGLAVLEAA
KTGKATAVHSGL

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol. Also shows some activity against structurally related compounds such as 3-keto-1,5-anhydro- D-fructose, D-glucosone and D-xylosone. The enzyme cannot use NADH as

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Homo sapiens, GI7657212, Length=281, Percent_Identity=25.6227758007117, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI87082405, Length=227, Percent_Identity=30.3964757709251, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787574, Length=326, Percent_Identity=27.3006134969325, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87081947, Length=255, Percent_Identity=27.0588235294118, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI145693182, Length=214, Percent_Identity=23.8317757009346, Blast_Score=65, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24581117, Length=287, Percent_Identity=26.1324041811847, Blast_Score=104, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24581115, Length=197, Percent_Identity=28.9340101522843, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24584727, Length=276, Percent_Identity=23.1884057971014, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 34954; Mature: 34823

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKWGLIGASTIAKEWVIGTIRATGGEVVSVYSTNAARGKTYADENGIARSVTSLDALLA
CCCCCCCCHHHHHHHHEEEEEEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
DAEIDAVYISTTNELHRDQAIAAAQAGKHILCEKPLALTIGDAHEMLKAARAAGVVAGTN
CCCCCEEEEECCHHHHHHHHHHHHHCCCEEEECCCCEEEECCHHHHHHHHHHCCEEECCC
HHLRNAASHRAMRDAVAEGRIGKVLGARVFHAVYLPPHLQGWRIERPDAGGGVVLDITVH
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCCEEECCCCCCCEEEEEEEE
DADTLRFVLGENPVEAVAFSQQGGLSGEGLEDGVMGVLRFPSGAIAQFHDAFTAKYAETG
CCCEEEEEECCCCHHEEEECCCCCCCCCCCCHHCEEEEECCCCHHHHHHHHHHHHHHHCC
FGVHGSEGSLIARNVMTQKPVGTVILRDKDGEHELPLDHKNLYETALQAFHDAIAGRGQP
CCEECCCCCEEEHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
SATLEDGIWSLATGLAVLEAAKTGKATAVHSGL
CCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCC
>Mature Secondary Structure 
AKWGLIGASTIAKEWVIGTIRATGGEVVSVYSTNAARGKTYADENGIARSVTSLDALLA
CCCCCCCHHHHHHHHEEEEEEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
DAEIDAVYISTTNELHRDQAIAAAQAGKHILCEKPLALTIGDAHEMLKAARAAGVVAGTN
CCCCCEEEEECCHHHHHHHHHHHHHCCCEEEECCCCEEEECCHHHHHHHHHHCCEEECCC
HHLRNAASHRAMRDAVAEGRIGKVLGARVFHAVYLPPHLQGWRIERPDAGGGVVLDITVH
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCCEEECCCCCCCEEEEEEEE
DADTLRFVLGENPVEAVAFSQQGGLSGEGLEDGVMGVLRFPSGAIAQFHDAFTAKYAETG
CCCEEEEEECCCCHHEEEECCCCCCCCCCCCHHCEEEEECCCCHHHHHHHHHHHHHHHCC
FGVHGSEGSLIARNVMTQKPVGTVILRDKDGEHELPLDHKNLYETALQAFHDAIAGRGQP
CCEECCCCCEEEHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
SATLEDGIWSLATGLAVLEAAKTGKATAVHSGL
CCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA