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The map label for this gene is cysU [H]

Identifier: 23502201

GI number: 23502201

Start: 1291615

End: 1292481

Strand: Reverse

Name: cysU [H]

Synonym: BR1329

Alternate gene names: 23502201

Gene position: 1292481-1291615 (Counterclockwise)

Preceding gene: 23502202

Following gene: 23502200

Centisome position: 61.32

GC content: 58.71

Gene sequence:

>867_bases
ATGCTCCCGTCGTGCTTCTGGAAGAATTCGGTGTCTCTGTTAAGTATGAAACGCAATTCTGTGCTGCCGGGATTCGGACT
GACCCTCGGCTGCACTCTGGTTTATCTCACGGTCATCGTTGCGCTTCCCTTGCTGGCGATGATCCTGAAGACAGCCAGCC
TGGGGTGGGGCGATTTCTGGAATATTGTCACCTCCGAGCGGGCGCTGGCGACCTATCGCATCACCGTGAGCGCCGCCGCT
GTCGCCACGATCCTCAATGGTTTGTTCGGCCTGCTGCTGGCCTGGGTGTTGACGCGCTACGACTTTCCCGGCAAGCGCCT
CATCGATGCGGCCGTTGATCTTCCCTTCGCCCTGCCGACGGCAATTGCCGGCCTTGCCCTTGTGACGCTCTTTGCGCAGA
ACGGCTGGTTCGGGCGTGTGCTGGAGCCGCTTGGCATCAAGGTTGCCTATGCGCCGCTCGGCATCATGATTGCGATGTTT
TTCACCAGCATCCCCTTTGTCGTTCGCACGGTGCAGCCGGTGCTTGAAGACATGAGCGCGGATGTGGAAGAGGCTGCGCG
CAGCCTTGGGGCTACTTCGTGGCAGACCTTCACCCATATCACCTGGCCGACCATTTTCCCGGCCTTTCTGGCGGGAGCCT
CGCTTTCGTTCGCGCGCAGCCTTGGCGAATTCGGTTCCATTGTCTTTATCTCCGGCAATCTTCCCTTTCAGACGGAAGTG
ACCGCGCTGCTGGTTTTCATCCGGCTTGATGAATATGATTATCCGGCGGCGGCGGCACTGGCCTTCGTGATGCTGGTGAT
GGCTTTTTTCATGCTTCTCGTGACCAATCTCATCCAGGCAAGGCGGCTTCGCTATGCAGAACGCTGA

Upstream 100 bases:

>100_bases
AACGGAAACGCTGGAACCGCTCCAAGTATTTGTTTTGTCGCATTATCCGACGCAAAACCGCTTCGCACTTTTGGCTCGAA
AATGCTCTAGGCGGGGCGTA

Downstream 100 bases:

>100_bases
CGCAGCCTTGCAGGCCCGCAACACGGGGCCAGTCCGCAACATTCTGATCTGGCTTGCAGCGGGTCTTTCGGTGCTTTGCA
TCGGTGCGCCGCTGGCGGTT

Product: sulfate ABC transporter permease

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MLPSCFWKNSVSLLSMKRNSVLPGFGLTLGCTLVYLTVIVALPLLAMILKTASLGWGDFWNIVTSERALATYRITVSAAA
VATILNGLFGLLLAWVLTRYDFPGKRLIDAAVDLPFALPTAIAGLALVTLFAQNGWFGRVLEPLGIKVAYAPLGIMIAMF
FTSIPFVVRTVQPVLEDMSADVEEAARSLGATSWQTFTHITWPTIFPAFLAGASLSFARSLGEFGSIVFISGNLPFQTEV
TALLVFIRLDEYDYPAAAALAFVMLVMAFFMLLVTNLIQARRLRYAER

Sequences:

>Translated_288_residues
MLPSCFWKNSVSLLSMKRNSVLPGFGLTLGCTLVYLTVIVALPLLAMILKTASLGWGDFWNIVTSERALATYRITVSAAA
VATILNGLFGLLLAWVLTRYDFPGKRLIDAAVDLPFALPTAIAGLALVTLFAQNGWFGRVLEPLGIKVAYAPLGIMIAMF
FTSIPFVVRTVQPVLEDMSADVEEAARSLGATSWQTFTHITWPTIFPAFLAGASLSFARSLGEFGSIVFISGNLPFQTEV
TALLVFIRLDEYDYPAAAALAFVMLVMAFFMLLVTNLIQARRLRYAER
>Mature_288_residues
MLPSCFWKNSVSLLSMKRNSVLPGFGLTLGCTLVYLTVIVALPLLAMILKTASLGWGDFWNIVTSERALATYRITVSAAA
VATILNGLFGLLLAWVLTRYDFPGKRLIDAAVDLPFALPTAIAGLALVTLFAQNGWFGRVLEPLGIKVAYAPLGIMIAMF
FTSIPFVVRTVQPVLEDMSADVEEAARSLGATSWQTFTHITWPTIFPAFLAGASLSFARSLGEFGSIVFISGNLPFQTEV
TALLVFIRLDEYDYPAAAALAFVMLVMAFFMLLVTNLIQARRLRYAER

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=269, Percent_Identity=51.6728624535316, Blast_Score=278, Evalue=2e-76,
Organism=Escherichia coli, GI87082099, Length=279, Percent_Identity=30.4659498207885, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1786980, Length=206, Percent_Identity=33.0097087378641, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786254, Length=227, Percent_Identity=23.7885462555066, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787369, Length=207, Percent_Identity=26.0869565217391, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31478; Mature: 31478

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPSCFWKNSVSLLSMKRNSVLPGFGLTLGCTLVYLTVIVALPLLAMILKTASLGWGDFW
CCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
NIVTSERALATYRITVSAAAVATILNGLFGLLLAWVLTRYDFPGKRLIDAAVDLPFALPT
HHHHCCHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHH
AIAGLALVTLFAQNGWFGRVLEPLGIKVAYAPLGIMIAMFFTSIPFVVRTVQPVLEDMSA
HHHHHHHHHHHHCCCCHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DVEEAARSLGATSWQTFTHITWPTIFPAFLAGASLSFARSLGEFGSIVFISGNLPFQTEV
CHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCHHHH
TALLVFIRLDEYDYPAAAALAFVMLVMAFFMLLVTNLIQARRLRYAER
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLPSCFWKNSVSLLSMKRNSVLPGFGLTLGCTLVYLTVIVALPLLAMILKTASLGWGDFW
CCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
NIVTSERALATYRITVSAAAVATILNGLFGLLLAWVLTRYDFPGKRLIDAAVDLPFALPT
HHHHCCHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHH
AIAGLALVTLFAQNGWFGRVLEPLGIKVAYAPLGIMIAMFFTSIPFVVRTVQPVLEDMSA
HHHHHHHHHHHHCCCCHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DVEEAARSLGATSWQTFTHITWPTIFPAFLAGASLSFARSLGEFGSIVFISGNLPFQTEV
CHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCHHHH
TALLVFIRLDEYDYPAAAALAFVMLVMAFFMLLVTNLIQARRLRYAER
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]