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The map label for this gene is cysW-2 [H]

Identifier: 23502200

GI number: 23502200

Start: 1290798

End: 1291628

Strand: Reverse

Name: cysW-2 [H]

Synonym: BR1328

Alternate gene names: 23502200

Gene position: 1291628-1290798 (Counterclockwise)

Preceding gene: 23502201

Following gene: 23502195

Centisome position: 61.28

GC content: 56.44

Gene sequence:

>831_bases
ATGCAGAACGCTGACGCAGCCTTGCAGGCCCGCAACACGGGGCCAGTCCGCAACATTCTGATCTGGCTTGCAGCGGGTCT
TTCGGTGCTTTGCATCGGTGCGCCGCTGGCGGTTATCTTCTCCTACGCCTTCAGCAAGGGCATCCATGTCTTCTTCAGCG
AGATATTGAAGCCGGATACACTCCATGCCGTGTGGCTCACCGTGCTGACCGCAATCGTTGTCGTACCGATCAACATGGCT
TTCGGCGTCTGCGTGGCGTGGCTGGTGACGAAATTCCGCTTTCCCGGACGCAGGCTCCTCATTACCTTCGTGGAGATACC
TTTTTCCGTTTCGCCCATCGTGGCGGGCGTGACCTATCTCTTCCTCTATGGTTCGCAAGGGCTTCTCGGGCCGTTTCTGG
AAGCGCATGATATCAAGATCATGTTCACGGTTCCGGCCATTTTTCTGGTAAGTCTTTTTGTGACTTCGCCTTTCGTGGCG
CGGGAGCTGATCCCGCTGATGGAAGTGCAGGGAAGCGATGAGGAGGAGGCGGCATTGACGCTGGGCGCAAGCGCCTGGCA
GACATTCTTCTATGTCACCTTGCCCAATATCAAATGGGCCTTGCTCTATGGCGCGGTGCTTTGCAATGCGCGTGTCATGG
GCGAGTTTGGCGCAACGTCCGTCGTGTCGGGCGCGATCCGCGGCAAGACCAATACATTGCCGCTTCAGGTTGAGCTGTTG
TTCAACGATTACAATGTGGCAGGCGCTTTTGCGGCAGCTTCCACGCTGGCGCTCATCGCGCTTGTAACACTGGTTCTAAA
GGTCTTTCTTGAACGCAAGCAGCATTCTTAG

Upstream 100 bases:

>100_bases
TATGATTATCCGGCGGCGGCGGCACTGGCCTTCGTGATGCTGGTGATGGCTTTTTTCATGCTTCTCGTGACCAATCTCAT
CCAGGCAAGGCGGCTTCGCT

Downstream 100 bases:

>100_bases
AGCATAATCCGGATCGTTTTGTTGCACTCCGGCTGGATTATGCTCTTAAAATGCCGCCCATCCCGGAAGGAATGGGCGGG
GAGCAGGCAGGCCGCCTGGG

Product: sulfate ABC transporter permease

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MQNADAALQARNTGPVRNILIWLAAGLSVLCIGAPLAVIFSYAFSKGIHVFFSEILKPDTLHAVWLTVLTAIVVVPINMA
FGVCVAWLVTKFRFPGRRLLITFVEIPFSVSPIVAGVTYLFLYGSQGLLGPFLEAHDIKIMFTVPAIFLVSLFVTSPFVA
RELIPLMEVQGSDEEEAALTLGASAWQTFFYVTLPNIKWALLYGAVLCNARVMGEFGATSVVSGAIRGKTNTLPLQVELL
FNDYNVAGAFAAASTLALIALVTLVLKVFLERKQHS

Sequences:

>Translated_276_residues
MQNADAALQARNTGPVRNILIWLAAGLSVLCIGAPLAVIFSYAFSKGIHVFFSEILKPDTLHAVWLTVLTAIVVVPINMA
FGVCVAWLVTKFRFPGRRLLITFVEIPFSVSPIVAGVTYLFLYGSQGLLGPFLEAHDIKIMFTVPAIFLVSLFVTSPFVA
RELIPLMEVQGSDEEEAALTLGASAWQTFFYVTLPNIKWALLYGAVLCNARVMGEFGATSVVSGAIRGKTNTLPLQVELL
FNDYNVAGAFAAASTLALIALVTLVLKVFLERKQHS
>Mature_276_residues
MQNADAALQARNTGPVRNILIWLAAGLSVLCIGAPLAVIFSYAFSKGIHVFFSEILKPDTLHAVWLTVLTAIVVVPINMA
FGVCVAWLVTKFRFPGRRLLITFVEIPFSVSPIVAGVTYLFLYGSQGLLGPFLEAHDIKIMFTVPAIFLVSLFVTSPFVA
RELIPLMEVQGSDEEEAALTLGASAWQTFFYVTLPNIKWALLYGAVLCNARVMGEFGATSVVSGAIRGKTNTLPLQVELL
FNDYNVAGAFAAASTLALIALVTLVLKVFLERKQHS

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=239, Percent_Identity=58.1589958158996, Blast_Score=296, Evalue=7e-82,
Organism=Escherichia coli, GI1788764, Length=206, Percent_Identity=36.8932038834951, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1786980, Length=223, Percent_Identity=30.4932735426009, Blast_Score=81, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29954; Mature: 29954

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNADAALQARNTGPVRNILIWLAAGLSVLCIGAPLAVIFSYAFSKGIHVFFSEILKPDT
CCCCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCH
LHAVWLTVLTAIVVVPINMAFGVCVAWLVTKFRFPGRRLLITFVEIPFSVSPIVAGVTYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHH
FLYGSQGLLGPFLEAHDIKIMFTVPAIFLVSLFVTSPFVARELIPLMEVQGSDEEEAALT
HHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHEE
LGASAWQTFFYVTLPNIKWALLYGAVLCNARVMGEFGATSVVSGAIRGKTNTLPLQVELL
ECHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEEEEE
FNDYNVAGAFAAASTLALIALVTLVLKVFLERKQHS
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQNADAALQARNTGPVRNILIWLAAGLSVLCIGAPLAVIFSYAFSKGIHVFFSEILKPDT
CCCCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCH
LHAVWLTVLTAIVVVPINMAFGVCVAWLVTKFRFPGRRLLITFVEIPFSVSPIVAGVTYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHH
FLYGSQGLLGPFLEAHDIKIMFTVPAIFLVSLFVTSPFVARELIPLMEVQGSDEEEAALT
HHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHEE
LGASAWQTFFYVTLPNIKWALLYGAVLCNARVMGEFGATSVVSGAIRGKTNTLPLQVELL
ECHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEEEEE
FNDYNVAGAFAAASTLALIALVTLVLKVFLERKQHS
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]