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The map label for this gene is cysP [H]

Identifier: 23502202

GI number: 23502202

Start: 1292608

End: 1293612

Strand: Reverse

Name: cysP [H]

Synonym: BR1330

Alternate gene names: 23502202

Gene position: 1293612-1292608 (Counterclockwise)

Preceding gene: 23502203

Following gene: 23502201

Centisome position: 61.37

GC content: 55.52

Gene sequence:

>1005_bases
ATGAGAAAAATTATTCTATACGCGGCAGTTGCGCTTGGTCTTGGTGCTGCGCCGGTTCAGGCTCAGGAGCCGAATGAAAT
CCTCAACGTCTCCTATGACATTGCGCGAGAGCTTTATGAGCAGATCAATCAGGCTTTTGTTGCTGACTGGAAGGCCAGGA
CCGGACAGGAGCTGACGGTCAACCAGTCCCATGCGGGTTCCTCCAAGCAGGCCCGCTCCATTCTGGAGGGGCTGGAAGCC
GATGTCGTGACCTTTAATCAGGTGGTGGACGTGCAGGTTCTGCACGACAAGGGCAATCTCATCCCGGCTGATTGGCAGAA
GCGGCTTCCCAATAATTCCTCGCCTTATTATTCTTTCCCGGCCTTTCTTGTCCGCGAAGGCAATCCGAAGCACATCAAGA
ACTGGGATGATCTGGCTCGTGACGATGTGAAGGTGATCTTCCCCAACCCGAAGACCTCCGGCAATGCGCGCTATACCTAT
CTTGCAGCCACGGCCTATGCCAACGAGGCTTTCAAGGGCGACAAGGAGAAGGTGAAGGAGTTTATCGGCAAGATTTTCAG
CAATGTGCCGGTGTTCGATACGGGCGGTCGCGGTGCGACCACGACTTTCGCCGAGCGTGGCCTTGGCGATGTGCTCGTGA
CTTTCGAGGCCGAGACGCGGGGCACGGAAAAAATGCTTGGGCCTGACAAATATGATGTGGTCGTTCCTGAAATGAGCCTG
CTTGCCGAATTCCCGGTCACGGTGGTCGATACGGTCGTGGACAAGCGCGGCTCACGCAGGATCGCCGAGGCCTATCTCGA
TTATCTTTATTCGCCCGCCGGGCAGGAGATTCTGGCCCAGAATTTCAATCGCGTGCACGACAAGGATGTGATCGCGAAGC
ACAAGGATATCTATCCCGATGTGCGCCTTGTGACGGTGGAAGAAGCGTTCGGCGGCTGGGACAAGGTTCAGAAGGAGCAT
TTCTCCGAAGGCGGTGTGCTCGATCAACTCTTTACCGGCAAGTAA

Upstream 100 bases:

>100_bases
GATTTAGATTGATTTTGCTTCATTTGCCGCTAGATTTGTTTTCTAAATTCAATTTGAAAACTACGCCCTTTGTACAGCGG
TGGCGGTGGGGAAGCATGTC

Downstream 100 bases:

>100_bases
TGGCGCAATAGAGCGGTTCCGGTTGAAACGGAAACGCTGGAACCGCTCCAAGTATTTGTTTTGTCGCATTATCCGACGCA
AAACCGCTTCGCACTTTTGG

Product: sulfate ABC transporter sulfate-binding protein

Products: SO42- [Cytoplasm]; phosphate; ADP; S2O32- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MRKIILYAAVALGLGAAPVQAQEPNEILNVSYDIARELYEQINQAFVADWKARTGQELTVNQSHAGSSKQARSILEGLEA
DVVTFNQVVDVQVLHDKGNLIPADWQKRLPNNSSPYYSFPAFLVREGNPKHIKNWDDLARDDVKVIFPNPKTSGNARYTY
LAATAYANEAFKGDKEKVKEFIGKIFSNVPVFDTGGRGATTTFAERGLGDVLVTFEAETRGTEKMLGPDKYDVVVPEMSL
LAEFPVTVVDTVVDKRGSRRIAEAYLDYLYSPAGQEILAQNFNRVHDKDVIAKHKDIYPDVRLVTVEEAFGGWDKVQKEH
FSEGGVLDQLFTGK

Sequences:

>Translated_334_residues
MRKIILYAAVALGLGAAPVQAQEPNEILNVSYDIARELYEQINQAFVADWKARTGQELTVNQSHAGSSKQARSILEGLEA
DVVTFNQVVDVQVLHDKGNLIPADWQKRLPNNSSPYYSFPAFLVREGNPKHIKNWDDLARDDVKVIFPNPKTSGNARYTY
LAATAYANEAFKGDKEKVKEFIGKIFSNVPVFDTGGRGATTTFAERGLGDVLVTFEAETRGTEKMLGPDKYDVVVPEMSL
LAEFPVTVVDTVVDKRGSRRIAEAYLDYLYSPAGQEILAQNFNRVHDKDVIAKHKDIYPDVRLVTVEEAFGGWDKVQKEH
FSEGGVLDQLFTGK
>Mature_334_residues
MRKIILYAAVALGLGAAPVQAQEPNEILNVSYDIARELYEQINQAFVADWKARTGQELTVNQSHAGSSKQARSILEGLEA
DVVTFNQVVDVQVLHDKGNLIPADWQKRLPNNSSPYYSFPAFLVREGNPKHIKNWDDLARDDVKVIFPNPKTSGNARYTY
LAATAYANEAFKGDKEKVKEFIGKIFSNVPVFDTGGRGATTTFAERGLGDVLVTFEAETRGTEKMLGPDKYDVVVPEMSL
LAEFPVTVVDTVVDKRGSRRIAEAYLDYLYSPAGQEILAQNFNRVHDKDVIAKHKDIYPDVRLVTVEEAFGGWDKVQKEH
FSEGGVLDQLFTGK

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. This protein specifically binds thiosulfate and is involved in its transmembrane transport [H]

COG id: COG4150

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1788765, Length=310, Percent_Identity=57.0967741935484, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1790351, Length=317, Percent_Identity=50.788643533123, Blast_Score=331, Evalue=4e-92,

Paralogues:

None

Copy number: 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37189; Mature: 37189

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIILYAAVALGLGAAPVQAQEPNEILNVSYDIARELYEQINQAFVADWKARTGQELTV
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
NQSHAGSSKQARSILEGLEADVVTFNQVVDVQVLHDKGNLIPADWQKRLPNNSSPYYSFP
ECCCCCCHHHHHHHHHHHCCCCEEHHHEEEEEEEECCCCCCCCCHHHHCCCCCCCCEECC
AFLVREGNPKHIKNWDDLARDDVKVIFPNPKTSGNARYTYLAATAYANEAFKGDKEKVKE
EEEEECCCCCCCCCHHHHCCCCEEEEECCCCCCCCCEEEEEEEEHHHHHHHCCCHHHHHH
FIGKIFSNVPVFDTGGRGATTTFAERGLGDVLVTFEAETRGTEKMLGPDKYDVVVPEMSL
HHHHHHCCCCEEECCCCCCCHHHHHCCCCCEEEEEECCCCCCHHHCCCCCCEEECCCHHH
LAEFPVTVVDTVVDKRGSRRIAEAYLDYLYSPAGQEILAQNFNRVHDKDVIAKHKDIYPD
HHHCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
VRLVTVEEAFGGWDKVQKEHFSEGGVLDQLFTGK
EEEEEEHHHHCCHHHHHHHHCCCCCCHHHHHCCC
>Mature Secondary Structure
MRKIILYAAVALGLGAAPVQAQEPNEILNVSYDIARELYEQINQAFVADWKARTGQELTV
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
NQSHAGSSKQARSILEGLEADVVTFNQVVDVQVLHDKGNLIPADWQKRLPNNSSPYYSFP
ECCCCCCHHHHHHHHHHHCCCCEEHHHEEEEEEEECCCCCCCCCHHHHCCCCCCCCEECC
AFLVREGNPKHIKNWDDLARDDVKVIFPNPKTSGNARYTYLAATAYANEAFKGDKEKVKE
EEEEECCCCCCCCCHHHHCCCCEEEEECCCCCCCCCEEEEEEEEHHHHHHHCCCHHHHHH
FIGKIFSNVPVFDTGGRGATTTFAERGLGDVLVTFEAETRGTEKMLGPDKYDVVVPEMSL
HHHHHHCCCCEEECCCCCCCHHHHHCCCCCEEEEEECCCCCCHHHCCCCCCEEECCCHHH
LAEFPVTVVDTVVDKRGSRRIAEAYLDYLYSPAGQEILAQNFNRVHDKDVIAKHKDIYPD
HHHCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
VRLVTVEEAFGGWDKVQKEHFSEGGVLDQLFTGK
EEEEEEHHHHCCHHHHHHHHCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: SO42- [Periplasm]; H2O; ATP; S2O32- [Periplasm] [C]

Specific reaction: SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2188959; 9205837; 9278503; 9298646 [H]