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The map label for this gene is polA [H]

Identifier: 23501034

GI number: 23501034

Start: 132325

End: 132942

Strand: Reverse

Name: polA [H]

Synonym: BR0120

Alternate gene names: 23501034

Gene position: 132942-132325 (Counterclockwise)

Preceding gene: 23501035

Following gene: 23501033

Centisome position: 6.31

GC content: 56.15

Gene sequence:

>618_bases
ATGACTATCCGTTTTCATAGGAACGACCTGCCGAACCTCGACAATTATCAGGTGGATGCGGTCGCCATCGATACAGAGAC
ACTGGGCCTGAACCCGCATCGCGACCGGCTTTGCGTGGTTCAGATTTCGCCCGGCGACGGTACTGCGGACGTAATCCAGA
TCGAAGCGGACCAGAAGAAGGCGCCCAATCTGGTGAAGCTTTTGAAAGATCGTTCCATCACCAAGATCTTTCATTTCGGC
CGGTTCGACCTTGCGGTTCTGGCACATGCATTCGGCACCATGCCGCAGCCGGTGTTCTGCACCAAGATCGCATCCAAACT
CACCCGCACCTATACGGATCGTCACGGCCTGAAGGAGATTTGCAGCGAGCTTCTGGACGTCTCGATTTCCAAGCAGCAGC
AATCTTCCGACTGGGCAGCCGAAGTGCTGTCCCAGGCGCAGCTTGAATATGCGGCATCGGATGTCCTCTACCTGCATCGC
CTGAAGGCCGTTCTGGAACAGCGTCTTGAACGTGATGGCCGCACGAAACAGGCCGAAGCCTGTTTCAAGTTCCTGCCCAC
GCGCTCGGAACTCGACCTCATGGGTTGGGCGGAATCCGATATCTTTGCTCATAGCTGA

Upstream 100 bases:

>100_bases
TCCATAAGGGCCAGCGCTCCTGGGCTCGTATTTTGGCGGCTATCGCCGCCAAGCAAGGCTGATATCATGGCGGCAATTGC
CGCCAAATAAGGACAATTGA

Downstream 100 bases:

>100_bases
GCAGAGATATTCAGGCGGTGCAAAGCCTCCAGCCTGGTTTCCATTCCGGCAAAACCATCTGCATTGACGCATCTGCAAAG
GCAAATCCGTCTCACATTCC

Product: 3'-5' exonuclease family protein

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MTIRFHRNDLPNLDNYQVDAVAIDTETLGLNPHRDRLCVVQISPGDGTADVIQIEADQKKAPNLVKLLKDRSITKIFHFG
RFDLAVLAHAFGTMPQPVFCTKIASKLTRTYTDRHGLKEICSELLDVSISKQQQSSDWAAEVLSQAQLEYAASDVLYLHR
LKAVLEQRLERDGRTKQAEACFKFLPTRSELDLMGWAESDIFAHS

Sequences:

>Translated_205_residues
MTIRFHRNDLPNLDNYQVDAVAIDTETLGLNPHRDRLCVVQISPGDGTADVIQIEADQKKAPNLVKLLKDRSITKIFHFG
RFDLAVLAHAFGTMPQPVFCTKIASKLTRTYTDRHGLKEICSELLDVSISKQQQSSDWAAEVLSQAQLEYAASDVLYLHR
LKAVLEQRLERDGRTKQAEACFKFLPTRSELDLMGWAESDIFAHS
>Mature_204_residues
TIRFHRNDLPNLDNYQVDAVAIDTETLGLNPHRDRLCVVQISPGDGTADVIQIEADQKKAPNLVKLLKDRSITKIFHFGR
FDLAVLAHAFGTMPQPVFCTKIASKLTRTYTDRHGLKEICSELLDVSISKQQQSSDWAAEVLSQAQLEYAASDVLYLHRL
KAVLEQRLERDGRTKQAEACFKFLPTRSELDLMGWAESDIFAHS

Specific function: Cleaves Multimeric tRNA Precursor At The Spacer Region. [C]

COG id: COG0349

COG function: function code J; Ribonuclease D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 3'-5' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1788105, Length=155, Percent_Identity=34.1935483870968, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR002298
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01612 3_5_exonuc; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 23170; Mature: 23038

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRFHRNDLPNLDNYQVDAVAIDTETLGLNPHRDRLCVVQISPGDGTADVIQIEADQKK
CEEEECCCCCCCCCCEEEEEEEEECEECCCCCCCCCEEEEEECCCCCCEEEEEEECCCCC
APNLVKLLKDRSITKIFHFGRFDLAVLAHAFGTMPQPVFCTKIASKLTRTYTDRHGLKEI
CCHHHHHHHCCCHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHH
CSELLDVSISKQQQSSDWAAEVLSQAQLEYAASDVLYLHRLKAVLEQRLERDGRTKQAEA
HHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
CFKFLPTRSELDLMGWAESDIFAHS
HHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TIRFHRNDLPNLDNYQVDAVAIDTETLGLNPHRDRLCVVQISPGDGTADVIQIEADQKK
EEEECCCCCCCCCCEEEEEEEEECEECCCCCCCCCEEEEEECCCCCCEEEEEEECCCCC
APNLVKLLKDRSITKIFHFGRFDLAVLAHAFGTMPQPVFCTKIASKLTRTYTDRHGLKEI
CCHHHHHHHCCCHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHH
CSELLDVSISKQQQSSDWAAEVLSQAQLEYAASDVLYLHRLKAVLEQRLERDGRTKQAEA
HHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
CFKFLPTRSELDLMGWAESDIFAHS
HHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]