Click here to switch to the map view.

The map label for this gene is alr2

Identifier: 23100681

GI number: 23100681

Start: 3362079

End: 3363257

Strand: Direct

Name: alr2

Synonym: OB3226

Alternate gene names: 23100681

Gene position: 3362079-3363257 (Clockwise)

Preceding gene: 23100680

Following gene: 23100683

Centisome position: 92.61

GC content: 40.29

Gene sequence:

>1179_bases
ATGATTCAAGGAAGTTATCGTGACACATGGACAGAAGTATCATTAGATGCCATCCATCACAATGTAACGGCGTTTAAAAA
CCATATTGATCATCATACAAAGTTAATGGCGGTTGTAAAAGCGGATGGATACGGACACGGTGCTGTAGAGGTTGCACAGG
AGGCTTTAACAGCAGGCGCCGATTATCTAGCTGTTGCTATCTTAGACGAAGCTATTCAATTACGAGATGCTGGAATTGAT
GCACCGTTGCTTGTACTTGGATACACACATCCCGATGGATTAAAAACAGCCATTGAACAGCAAATTACACTCACAGTTTT
CACAAAAGAAGATGCAGAACAAGTGAAAATAGCTGCTGAGCTTCTGCAAAAGACAGCTCGCATACACCTAAAAATCGAAT
CTGGTATGAATCGGATCGGCATCGCTACGAAAGAAGAAGCAGTTGAAATAGCTAAAGCACTTCATTCTTCTTTTGTTATG
TTAGAAGGTGCATTTACACACTTTGCCGATGCGGATAATACAGATCCCACTTATACAGAGATGCAATTTCACCGTTTTAA
TCAGATTATTTCTCATTTAAGAACGCATTGCCATATCCCTATTGTACATTGCTGTAATACTGCTGCGACAATAGCTTACC
CGGATATGCATTTGGATATGGTTCGTGTTGGTATCGGCATCTATGGATTATACCCAGAAACACATTTGAAAGAATTGATT
GATTTAAAACAAGCAATGAGTCTAAAAACAAAACCTGTTTATATAAAAACAGTTGATCAGGACACCGCAATTAGTTATGG
TCTTACATTCACGACAGAGCGAAAGAGCACCATTGCAACAATGCCAATTGGTTATGCAGATGGCTTTTCTCGACTACTGT
CTAATCGAGGAGATGTGATTGTACATGCCGGCCGCGCTCCTATTGTTGGACGAATTTGTATGGATCAATCGATGATCGAT
GTTACGGACGTGGAGAATGTTGGACTAGATGATGTAATTACCATTTTTGGTGAGCCTACCGAAGGATATATCGCGATGGA
AGAAGTAGCTAATCTGATGGGGACGATACATTATGAAACAGCATGCCTAATTGGAAAGCGTGTTCCACGGATATATATGA
GGCAAAGTGAAGCAGTTAATTATAAAGGACTAGTAGAGAAAGAACCTGTTACGTCTTAG

Upstream 100 bases:

>100_bases
TGCTATAAGCTTAGAAGGGTACTTTTATTCTAAGTGAAGAAATTATAGTGTAATTACCTTCAACTAACTATATTACCCAG
TCATCTAGGGAGGAATCACA

Downstream 100 bases:

>100_bases
TTTCTTGTTGAACAATATATTTCAGATTAATTATAGGCGGAAACTCTTGTAGAAAATCGGCTTTCAGTCCGTGCTGATCC
TACTGGAGTCTCCGTCTTTT

Product: D-alanine racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MIQGSYRDTWTEVSLDAIHHNVTAFKNHIDHHTKLMAVVKADGYGHGAVEVAQEALTAGADYLAVAILDEAIQLRDAGID
APLLVLGYTHPDGLKTAIEQQITLTVFTKEDAEQVKIAAELLQKTARIHLKIESGMNRIGIATKEEAVEIAKALHSSFVM
LEGAFTHFADADNTDPTYTEMQFHRFNQIISHLRTHCHIPIVHCCNTAATIAYPDMHLDMVRVGIGIYGLYPETHLKELI
DLKQAMSLKTKPVYIKTVDQDTAISYGLTFTTERKSTIATMPIGYADGFSRLLSNRGDVIVHAGRAPIVGRICMDQSMID
VTDVENVGLDDVITIFGEPTEGYIAMEEVANLMGTIHYETACLIGKRVPRIYMRQSEAVNYKGLVEKEPVTS

Sequences:

>Translated_392_residues
MIQGSYRDTWTEVSLDAIHHNVTAFKNHIDHHTKLMAVVKADGYGHGAVEVAQEALTAGADYLAVAILDEAIQLRDAGID
APLLVLGYTHPDGLKTAIEQQITLTVFTKEDAEQVKIAAELLQKTARIHLKIESGMNRIGIATKEEAVEIAKALHSSFVM
LEGAFTHFADADNTDPTYTEMQFHRFNQIISHLRTHCHIPIVHCCNTAATIAYPDMHLDMVRVGIGIYGLYPETHLKELI
DLKQAMSLKTKPVYIKTVDQDTAISYGLTFTTERKSTIATMPIGYADGFSRLLSNRGDVIVHAGRAPIVGRICMDQSMID
VTDVENVGLDDVITIFGEPTEGYIAMEEVANLMGTIHYETACLIGKRVPRIYMRQSEAVNYKGLVEKEPVTS
>Mature_392_residues
MIQGSYRDTWTEVSLDAIHHNVTAFKNHIDHHTKLMAVVKADGYGHGAVEVAQEALTAGADYLAVAILDEAIQLRDAGID
APLLVLGYTHPDGLKTAIEQQITLTVFTKEDAEQVKIAAELLQKTARIHLKIESGMNRIGIATKEEAVEIAKALHSSFVM
LEGAFTHFADADNTDPTYTEMQFHRFNQIISHLRTHCHIPIVHCCNTAATIAYPDMHLDMVRVGIGIYGLYPETHLKELI
DLKQAMSLKTKPVYIKTVDQDTAISYGLTFTTERKSTIATMPIGYADGFSRLLSNRGDVIVHAGRAPIVGRICMDQSMID
VTDVENVGLDDVITIFGEPTEGYIAMEEVANLMGTIHYETACLIGKRVPRIYMRQSEAVNYKGLVEKEPVTS

Specific function: Provides the D-alanine required for cell wall biosynthesis

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family

Homologues:

Organism=Escherichia coli, GI1787439, Length=362, Percent_Identity=28.7292817679558, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1790487, Length=371, Percent_Identity=26.9541778975741, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ALR2_OCEIH (Q8ELK1)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_694148.1
- ProteinModelPortal:   Q8ELK1
- SMR:   Q8ELK1
- GeneID:   1018058
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB3226
- NMPDR:   fig|221109.1.peg.3228
- HOGENOM:   HBG712172
- OMA:   GHKLPIV
- ProtClustDB:   CLSK302699
- BioCyc:   OIHE221109:OB3226-MONOMER
- BRENDA:   5.1.1.1
- HAMAP:   MF_01201
- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622
- Gene3D:   G3DSA:2.40.37.10
- PRINTS:   PR00992
- TIGRFAMs:   TIGR00492

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N; SSF50621 Racem_decarbox_C

EC number: =5.1.1.1

Molecular weight: Translated: 43322; Mature: 43322

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: ACT_SITE 40-40 ACT_SITE 266-266

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQGSYRDTWTEVSLDAIHHNVTAFKNHIDHHTKLMAVVKADGYGHGAVEVAQEALTAGA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHCCC
DYLAVAILDEAIQLRDAGIDAPLLVLGYTHPDGLKTAIEQQITLTVFTKEDAEQVKIAAE
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCEEEEEEEECCCHHHHHHHHH
LLQKTARIHLKIESGMNRIGIATKEEAVEIAKALHSSFVMLEGAFTHFADADNTDPTYTE
HHHHHHEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHEEEEECCHHHCCCCCCCCCCHHH
MQFHRFNQIISHLRTHCHIPIVHCCNTAATIAYPDMHLDMVRVGIGIYGLYPETHLKELI
HHHHHHHHHHHHHHHHCCCCCEECCCCCCEEECCCCHHHHHHHCCCEEECCCHHHHHHHH
DLKQAMSLKTKPVYIKTVDQDTAISYGLTFTTERKSTIATMPIGYADGFSRLLSNRGDVI
HHHHHHCCCCCCEEEEECCCCCEEEECEEEEECCCCEEEEECCCHHHHHHHHHCCCCCEE
VHAGRAPIVGRICMDQSMIDVTDVENVGLDDVITIFGEPTEGYIAMEEVANLMGTIHYET
EECCCCCEEEHHHCCCCCEEECCCCCCCCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHH
ACLIGKRVPRIYMRQSEAVNYKGLVEKEPVTS
HHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIQGSYRDTWTEVSLDAIHHNVTAFKNHIDHHTKLMAVVKADGYGHGAVEVAQEALTAGA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHCCC
DYLAVAILDEAIQLRDAGIDAPLLVLGYTHPDGLKTAIEQQITLTVFTKEDAEQVKIAAE
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCEEEEEEEECCCHHHHHHHHH
LLQKTARIHLKIESGMNRIGIATKEEAVEIAKALHSSFVMLEGAFTHFADADNTDPTYTE
HHHHHHEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHEEEEECCHHHCCCCCCCCCCHHH
MQFHRFNQIISHLRTHCHIPIVHCCNTAATIAYPDMHLDMVRVGIGIYGLYPETHLKELI
HHHHHHHHHHHHHHHHCCCCCEECCCCCCEEECCCCHHHHHHHCCCEEECCCHHHHHHHH
DLKQAMSLKTKPVYIKTVDQDTAISYGLTFTTERKSTIATMPIGYADGFSRLLSNRGDVI
HHHHHHCCCCCCEEEEECCCCCEEEECEEEEECCCCEEEEECCCHHHHHHHHHCCCCCEE
VHAGRAPIVGRICMDQSMIDVTDVENVGLDDVITIFGEPTEGYIAMEEVANLMGTIHYET
EECCCCCEEEHHHCCCCCEEECCCCCCCCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHH
ACLIGKRVPRIYMRQSEAVNYKGLVEKEPVTS
HHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376