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The map label for this gene is ald

Identifier: 23100680

GI number: 23100680

Start: 3360750

End: 3361880

Strand: Direct

Name: ald

Synonym: OB3225

Alternate gene names: 23100680

Gene position: 3360750-3361880 (Clockwise)

Preceding gene: 23100679

Following gene: 23100681

Centisome position: 92.57

GC content: 40.14

Gene sequence:

>1131_bases
ATGATTATTGGTGTTCCTAAGGAAATTAAAAACAATGAGAATCGTGTTGCAATGACACCTGCTGGGGTCGTTCATTTAAT
AAATGCTGGTCATACTGTACAAATTGAGAAAGGTGCAGGACTAGGAAGTAATTTTGCAGATGCAGAATACAAAGAAGCTG
GTGCTGAACTTATCGATAGCGCTGCTAGTGTTTGGGAAAATGCAGATATGATTATGAAGGTTAAAGAACCACTTTCTTCT
GAATATAAATACTTCCGTAAAGGTCTTATTTTATTCACTTACCTTCACCTTGCTGCTGCTCCAGAATTAACAAAAGCACT
AGTTGACAGTGAAGTAACTGCAATTGCTTATGAAACAATTACAGTAAACGGTACATTGCCACTATTAACTCCAATGAGTG
AAGTAGCAGGACGTATGGCTACACAAATCGGTGCACAGTATCTAGAGAAATCAGAAGGCGGAAAAGGTATCCTTCTTGGA
GGAATTCCAGGAGTTAGCCGCGGTAAAGTAACTGTAATCGGTGGTGGAGTTGTTGGTACGCACGCTGCTAAAATCGCTCT
AGGTCTTGGGGCAGAAGTAACGATTATTGACTTAAACCCTGTACGCCTTCGTCAATTAGACGATATCTTTGGTTCAAGCA
TTCAAACATTAATGTCTAACCCGTACAACATTGCAGAAGCTGTAAAAGATTCTGATCTAGTTATCGGTTCTGTATTAATC
CCAGGACGTAAAGCTCCTAAATTAGTTACAGACGAAATGATTCAATCGATGCAACCAGGATCTGTTCTTGTGGACGTTGC
AATTGACCAAGGTGGTAACTTCGAAACAGTAGATCACCCAACTACACATGATGAGCCAATCTATGTAAAACATGATGTAC
TTCATTATGCAGTTGCAAACATCCCTGGTGCGGTACCACGTACAGCTACTGTTGGATTAACTAACGTAACAGTGCCATAT
GCGGTACAAATCGCTTCTAAAGGTGCAGTAAAAGCAATTCAAGACAACCCAGCAATTCTTACTGGTGTAAACGTTATGAA
TGGTAAAGTAACTTACGAAGCAGTTGCTGCTGACCTTGGATACGACTTTGTTTCACCTGAAGATGCAATCAAAGACGCAG
AATTAGTTTAA

Upstream 100 bases:

>100_bases
TACACAAATCATGTTTTTTTCTTTTATATTTTTTAATGATGAAACCGCTTCAAGAAAGCTGTACGATAAGGTTAGAAATT
GATAAAGGGGTTGTTTATAG

Downstream 100 bases:

>100_bases
TTTTAATTGTTAAAATAAAGGTTGAAAATGAAAAAGCTAGCCTACCCAATATTTCATCTTTACCTTACTTTTAACGAATC
CATTTCAAGTACCCTTCCTG

Product: stage V sporulation protein N

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MIIGVPKEIKNNENRVAMTPAGVVHLINAGHTVQIEKGAGLGSNFADAEYKEAGAELIDSAASVWENADMIMKVKEPLSS
EYKYFRKGLILFTYLHLAAAPELTKALVDSEVTAIAYETITVNGTLPLLTPMSEVAGRMATQIGAQYLEKSEGGKGILLG
GIPGVSRGKVTVIGGGVVGTHAAKIALGLGAEVTIIDLNPVRLRQLDDIFGSSIQTLMSNPYNIAEAVKDSDLVIGSVLI
PGRKAPKLVTDEMIQSMQPGSVLVDVAIDQGGNFETVDHPTTHDEPIYVKHDVLHYAVANIPGAVPRTATVGLTNVTVPY
AVQIASKGAVKAIQDNPAILTGVNVMNGKVTYEAVAADLGYDFVSPEDAIKDAELV

Sequences:

>Translated_376_residues
MIIGVPKEIKNNENRVAMTPAGVVHLINAGHTVQIEKGAGLGSNFADAEYKEAGAELIDSAASVWENADMIMKVKEPLSS
EYKYFRKGLILFTYLHLAAAPELTKALVDSEVTAIAYETITVNGTLPLLTPMSEVAGRMATQIGAQYLEKSEGGKGILLG
GIPGVSRGKVTVIGGGVVGTHAAKIALGLGAEVTIIDLNPVRLRQLDDIFGSSIQTLMSNPYNIAEAVKDSDLVIGSVLI
PGRKAPKLVTDEMIQSMQPGSVLVDVAIDQGGNFETVDHPTTHDEPIYVKHDVLHYAVANIPGAVPRTATVGLTNVTVPY
AVQIASKGAVKAIQDNPAILTGVNVMNGKVTYEAVAADLGYDFVSPEDAIKDAELV
>Mature_376_residues
MIIGVPKEIKNNENRVAMTPAGVVHLINAGHTVQIEKGAGLGSNFADAEYKEAGAELIDSAASVWENADMIMKVKEPLSS
EYKYFRKGLILFTYLHLAAAPELTKALVDSEVTAIAYETITVNGTLPLLTPMSEVAGRMATQIGAQYLEKSEGGKGILLG
GIPGVSRGKVTVIGGGVVGTHAAKIALGLGAEVTIIDLNPVRLRQLDDIFGSSIQTLMSNPYNIAEAVKDSDLVIGSVLI
PGRKAPKLVTDEMIQSMQPGSVLVDVAIDQGGNFETVDHPTTHDEPIYVKHDVLHYAVANIPGAVPRTATVGLTNVTVPY
AVQIASKGAVKAIQDNPAILTGVNVMNGKVTYEAVAADLGYDFVSPEDAIKDAELV

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family

Homologues:

Organism=Homo sapiens, GI122939155, Length=344, Percent_Identity=30.8139534883721, Blast_Score=148, Evalue=7e-36,
Organism=Homo sapiens, GI122939153, Length=344, Percent_Identity=30.8139534883721, Blast_Score=148, Evalue=7e-36,
Organism=Escherichia coli, GI1787887, Length=379, Percent_Identity=31.3984168865435, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17550456, Length=332, Percent_Identity=31.3253012048193, Blast_Score=144, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DHA_OCEIH (Q8CX61)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_694147.1
- ProteinModelPortal:   Q8CX61
- SMR:   Q8CX61
- GeneID:   1016523
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB3225
- NMPDR:   fig|221109.1.peg.3227
- HOGENOM:   HBG289735
- OMA:   MIATMRT
- BioCyc:   OIHE221109:OB3225-MONOMER
- BRENDA:   1.4.1.1
- GO:   GO:0005737
- GO:   GO:0005488
- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141
- PIRSF:   PIRSF000183
- TIGRFAMs:   TIGR00518

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N

EC number: =1.4.1.1

Molecular weight: Translated: 39620; Mature: 39620

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00836 ALADH_PNT_1; PS00837 ALADH_PNT_2

Important sites: ACT_SITE 96-96

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIGVPKEIKNNENRVAMTPAGVVHLINAGHTVQIEKGAGLGSNFADAEYKEAGAELIDS
CEECCCHHHCCCCCEEEECCCCEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHH
AASVWENADMIMKVKEPLSSEYKYFRKGLILFTYLHLAAAPELTKALVDSEVTAIAYETI
HHHHHCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEEEEE
TVNGTLPLLTPMSEVAGRMATQIGAQYLEKSEGGKGILLGGIPGVSRGKVTVIGGGVVGT
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCEEHH
HAAKIALGLGAEVTIIDLNPVRLRQLDDIFGSSIQTLMSNPYNIAEAVKDSDLVIGSVLI
HHHHEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEEEE
PGRKAPKLVTDEMIQSMQPGSVLVDVAIDQGGNFETVDHPTTHDEPIYVKHDVLHYAVAN
CCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHC
IPGAVPRTATVGLTNVTVPYAVQIASKGAVKAIQDNPAILTGVNVMNGKVTYEAVAADLG
CCCCCCCCEEEEEEECCCCEEEEEECCCCEEEECCCCCEEEEEEEECCEEEHHHHHHHHC
YDFVSPEDAIKDAELV
CCCCCHHHHCCCCCCC
>Mature Secondary Structure
MIIGVPKEIKNNENRVAMTPAGVVHLINAGHTVQIEKGAGLGSNFADAEYKEAGAELIDS
CEECCCHHHCCCCCEEEECCCCEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHH
AASVWENADMIMKVKEPLSSEYKYFRKGLILFTYLHLAAAPELTKALVDSEVTAIAYETI
HHHHHCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEEEEE
TVNGTLPLLTPMSEVAGRMATQIGAQYLEKSEGGKGILLGGIPGVSRGKVTVIGGGVVGT
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCEEHH
HAAKIALGLGAEVTIIDLNPVRLRQLDDIFGSSIQTLMSNPYNIAEAVKDSDLVIGSVLI
HHHHEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEEEE
PGRKAPKLVTDEMIQSMQPGSVLVDVAIDQGGNFETVDHPTTHDEPIYVKHDVLHYAVAN
CCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHC
IPGAVPRTATVGLTNVTVPYAVQIASKGAVKAIQDNPAILTGVNVMNGKVTYEAVAADLG
CCCCCCCCEEEEEEECCCCEEEEEECCCCEEEECCCCCEEEEEEEECCEEEHHHHHHHHC
YDFVSPEDAIKDAELV
CCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376