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The map label for this gene is nifU [H]

Identifier: 23099832

GI number: 23099832

Start: 2429934

End: 2430362

Strand: Reverse

Name: nifU [H]

Synonym: OB2377

Alternate gene names: 23099832

Gene position: 2430362-2429934 (Counterclockwise)

Preceding gene: 23099833

Following gene: 23099831

Centisome position: 66.94

GC content: 38.23

Gene sequence:

>429_bases
ATGTCTTTGAATAACCTCGATACACTTTACCGACAAGTAATAATGGATCATTATAAGAATCCTAGAAATCGTGGTAGTCT
TGAAGGAGACTCTGTTACAGTAGATATGAATAACCCGACTTGTGGTGACCGAATTCAACTTCAGTTACATGTTGAAGATG
GAATTGTTCAAGACGCGAAGTATGAAGGAGAAGGTTGTTCAATCAGTATGTCATCGGCTTCTATGATGACACAAGCGATT
AAAGGGAAGAAGATAGAGGAAGCATTAGAAATGTCACAAACATTTTCCGATATGATGCTGGGAAATGATGTAGATACTGA
AGAAATGGGAGATATTGAAGCATTACAAGGTGTTTCTAAATTCCCTGCAAGAATAAAGTGTGCGACCTTAGCCTGGAAGG
CAATGGAAAAAGGCGTACATGATCAATAA

Upstream 100 bases:

>100_bases
GTTTCAGCGACGGCTAGAGCAAGTTTCTACCTTTATAATTCAAAAGAAGATGTAGATCGATTGGTAGAAGGACTGTTAAA
AACAAAGGAGTATTTTGGGA

Downstream 100 bases:

>100_bases
AGCAGAGATGTTTTTTCTGCTAGGAGGAGGTTATCTATATGGCTAAAAATATGCCAGAAATCGATGAGTATAAATATGGA
TTTCATGATAAAGACGTATC

Product: nitrogen fixation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 142; Mature: 141

Protein sequence:

>142_residues
MSLNNLDTLYRQVIMDHYKNPRNRGSLEGDSVTVDMNNPTCGDRIQLQLHVEDGIVQDAKYEGEGCSISMSSASMMTQAI
KGKKIEEALEMSQTFSDMMLGNDVDTEEMGDIEALQGVSKFPARIKCATLAWKAMEKGVHDQ

Sequences:

>Translated_142_residues
MSLNNLDTLYRQVIMDHYKNPRNRGSLEGDSVTVDMNNPTCGDRIQLQLHVEDGIVQDAKYEGEGCSISMSSASMMTQAI
KGKKIEEALEMSQTFSDMMLGNDVDTEEMGDIEALQGVSKFPARIKCATLAWKAMEKGVHDQ
>Mature_141_residues
SLNNLDTLYRQVIMDHYKNPRNRGSLEGDSVTVDMNNPTCGDRIQLQLHVEDGIVQDAKYEGEGCSISMSSASMMTQAIK
GKKIEEALEMSQTFSDMMLGNDVDTEEMGDIEALQGVSKFPARIKCATLAWKAMEKGVHDQ

Specific function: May be involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins (Potential) [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI56699456, Length=90, Percent_Identity=42.2222222222222, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI24307953, Length=85, Percent_Identity=42.3529411764706, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1788878, Length=136, Percent_Identity=31.6176470588235, Blast_Score=77, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17543474, Length=136, Percent_Identity=30.8823529411765, Blast_Score=69, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6324800, Length=138, Percent_Identity=30.4347826086957, Blast_Score=77, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6325122, Length=138, Percent_Identity=28.2608695652174, Blast_Score=70, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21355597, Length=91, Percent_Identity=41.7582417582418, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002871
- InterPro:   IPR011341 [H]

Pfam domain/function: PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 15766; Mature: 15635

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
7.7 %Met     (Translated Protein)
9.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
7.1 %Met     (Mature Protein)
9.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNNLDTLYRQVIMDHYKNPRNRGSLEGDSVTVDMNNPTCGDRIQLQLHVEDGIVQDAK
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCCCCCC
YEGEGCSISMSSASMMTQAIKGKKIEEALEMSQTFSDMMLGNDVDTEEMGDIEALQGVSK
CCCCCCEEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHH
FPARIKCATLAWKAMEKGVHDQ
CCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SLNNLDTLYRQVIMDHYKNPRNRGSLEGDSVTVDMNNPTCGDRIQLQLHVEDGIVQDAK
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCCCCCC
YEGEGCSISMSSASMMTQAIKGKKIEEALEMSQTFSDMMLGNDVDTEEMGDIEALQGVSK
CCCCCCEEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHH
FPARIKCATLAWKAMEKGVHDQ
CCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]