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The map label for this gene is ytzG [H]

Identifier: 23099754

GI number: 23099754

Start: 2344047

End: 2344760

Strand: Reverse

Name: ytzG [H]

Synonym: OB2299

Alternate gene names: 23099754

Gene position: 2344760-2344047 (Counterclockwise)

Preceding gene: 23099755

Following gene: 23099753

Centisome position: 64.58

GC content: 34.31

Gene sequence:

>714_bases
ATGATGCGTCTAGATAAATTACTCTCTAACATGGGAGTTGGTTCTCGGAAAGAAGTGAAACAACTCCTAAAATCAAAGCG
AATAACAGTTAATGAAGTTATAGTAAAAGACAGTGGAATGCATGTAGACCCGGAGTATGACCAAATATTTGTTGATGATC
AATTAATATCTTATCAAAAACATATTTACCTTATGATGCATAAGCCGCCTGGTGTAGTATCTGCGACTGTCGATAATCGA
GATCGTACTGTAATTGACTTATTATCAGCCGATGAGAAGAAGTTCGAACCGTTCCCTGTAGGTAGACTCGATAAAGATAC
AGAAGGACTTCTATTAATTACGAACGATGGTGAACTTGCTCATCGATTAACCTCACCAAAAAAACATGTCGAAAAGGTAT
ATATTGCTCATATTAGTGGTGAATTCCCGGAAAATATTGTTGAACAATTTCAAGAGGGTTTAACTTTAGAGGATGGATAT
CAAACGAAACCAGCTAAATTAGAAATATTAAATAAACAATCAAAGGAAGTAAAAGTTACAATAACAGAAGGGAAATATCA
TCAAATTAAACGAATGTTTATCGCTGTCGGGTGTAAAGTGACTTATTTAAAAAGGGTTCAGATGGCAAACTTACAATTAG
ATGATAGCTTGCCAATAGGTGATTTTCGTCCTTTAACGGAAGATGAATTACAGTATTGTAAATCATTAAATTAG

Upstream 100 bases:

>100_bases
GAGTCTACCTACTATTAGCTTATAAATCAACATTATTAGATCATGTGTTTGATGGAAAAGTACCGTTGCTTGGCAAATTT
ATGAAAAGATAGGAGAGAGA

Downstream 100 bases:

>100_bases
CTAAAGTAAAAAGGAGATGGAAATATTGTCTCATTATTTTATCGGTATAGACTTGCCTAATCATGTAAAACAAAACTTAA
TAGAAGTTCAGCAAAAACTT

Product: 16S pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MMRLDKLLSNMGVGSRKEVKQLLKSKRITVNEVIVKDSGMHVDPEYDQIFVDDQLISYQKHIYLMMHKPPGVVSATVDNR
DRTVIDLLSADEKKFEPFPVGRLDKDTEGLLLITNDGELAHRLTSPKKHVEKVYIAHISGEFPENIVEQFQEGLTLEDGY
QTKPAKLEILNKQSKEVKVTITEGKYHQIKRMFIAVGCKVTYLKRVQMANLQLDDSLPIGDFRPLTEDELQYCKSLN

Sequences:

>Translated_237_residues
MMRLDKLLSNMGVGSRKEVKQLLKSKRITVNEVIVKDSGMHVDPEYDQIFVDDQLISYQKHIYLMMHKPPGVVSATVDNR
DRTVIDLLSADEKKFEPFPVGRLDKDTEGLLLITNDGELAHRLTSPKKHVEKVYIAHISGEFPENIVEQFQEGLTLEDGY
QTKPAKLEILNKQSKEVKVTITEGKYHQIKRMFIAVGCKVTYLKRVQMANLQLDDSLPIGDFRPLTEDELQYCKSLN
>Mature_237_residues
MMRLDKLLSNMGVGSRKEVKQLLKSKRITVNEVIVKDSGMHVDPEYDQIFVDDQLISYQKHIYLMMHKPPGVVSATVDNR
DRTVIDLLSADEKKFEPFPVGRLDKDTEGLLLITNDGELAHRLTSPKKHVEKVYIAHISGEFPENIVEQFQEGLTLEDGY
QTKPAKLEILNKQSKEVKVTITEGKYHQIKRMFIAVGCKVTYLKRVQMANLQLDDSLPIGDFRPLTEDELQYCKSLN

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=230, Percent_Identity=37.8260869565217, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1787524, Length=246, Percent_Identity=32.520325203252, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1790453, Length=249, Percent_Identity=29.3172690763052, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI87081838, Length=185, Percent_Identity=30.8108108108108, Blast_Score=85, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27145; Mature: 27145

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRLDKLLSNMGVGSRKEVKQLLKSKRITVNEVIVKDSGMHVDPEYDQIFVDDQLISYQK
CCHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEEECCCCEECCCCCEEEECHHHHHHEE
HIYLMMHKPPGVVSATVDNRDRTVIDLLSADEKKFEPFPVGRLDKDTEGLLLITNDGELA
EEEEEEECCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCHHH
HRLTSPKKHVEKVYIAHISGEFPENIVEQFQEGLTLEDGYQTKPAKLEILNKQSKEVKVT
HHHCCCHHHHHEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCEEEEE
ITEGKYHQIKRMFIAVGCKVTYLKRVQMANLQLDDSLPIGDFRPLTEDELQYCKSLN
EECCHHHHHHHHHHHHCCEEEHHHHHHHHCEEECCCCCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MMRLDKLLSNMGVGSRKEVKQLLKSKRITVNEVIVKDSGMHVDPEYDQIFVDDQLISYQK
CCHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEEECCCCEECCCCCEEEECHHHHHHEE
HIYLMMHKPPGVVSATVDNRDRTVIDLLSADEKKFEPFPVGRLDKDTEGLLLITNDGELA
EEEEEEECCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCHHH
HRLTSPKKHVEKVYIAHISGEFPENIVEQFQEGLTLEDGYQTKPAKLEILNKQSKEVKVT
HHHCCCHHHHHEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCEEEEE
ITEGKYHQIKRMFIAVGCKVTYLKRVQMANLQLDDSLPIGDFRPLTEDELQYCKSLN
EECCHHHHHHHHHHHHCCEEEHHHHHHHHCEEECCCCCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]