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The map label for this gene is ligT [C]

Identifier: 23099753

GI number: 23099753

Start: 2343461

End: 2344030

Strand: Reverse

Name: ligT [C]

Synonym: OB2298

Alternate gene names: 23099753

Gene position: 2344030-2343461 (Counterclockwise)

Preceding gene: 23099754

Following gene: 23099752

Centisome position: 64.56

GC content: 32.28

Gene sequence:

>570_bases
ATGGAAATATTGTCTCATTATTTTATCGGTATAGACTTGCCTAATCATGTAAAACAAAACTTAATAGAAGTTCAGCAAAA
ACTTCCATCCTCCTTTTCTTATAAACAATGGACACATAAATTTGACTTTCACATAACACTAACATTTCTAGGTGAAATAG
ACGATAAACAATTAACGAACGTAGAGCAATATCTAAATAATTTGGAAAAGAAGCCTTTTTTTCTAACCCTGGATCAGTTA
GGTGTATTTGGTAATCAAAAGCAACCGAGAGTTCTATGGGCTGGTCTGGAACATTCTAATGAATTACATGAACTTCATCA
TCAGATTAGTCATATGTTAACACAGCTAGAATTATCTAAAGATAATCGGCCATATCGACCGCATATTACTTTAGCGAAGA
AATGGAATGATTCAACTATTCTCTTGGATAGTAACCAATTGGATCGCATGCAACAGATTGGACAATCTGTTGAAACGAGT
TTTGAAGTAGAAACCATTCATTTATTTAAAATTTATCCACAACAAATGCCAAAATACCAAAAATATAGGAGTTTTTCATT
ACGTGGTTAA

Upstream 100 bases:

>100_bases
TTACAATTAGATGATAGCTTGCCAATAGGTGATTTTCGTCCTTTAACGGAAGATGAATTACAGTATTGTAAATCATTAAA
TTAGCTAAAGTAAAAAGGAG

Downstream 100 bases:

>100_bases
GGGGTTGTATATATGGCGCAATTAATTAAATTACAAGATTATATATCACGTTATGAGTGGGATACTTATCGTTATCCTAC
GCGATACATACGTCAAAAAA

Product: ABC transporter permease

Products: NA

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MEILSHYFIGIDLPNHVKQNLIEVQQKLPSSFSYKQWTHKFDFHITLTFLGEIDDKQLTNVEQYLNNLEKKPFFLTLDQL
GVFGNQKQPRVLWAGLEHSNELHELHHQISHMLTQLELSKDNRPYRPHITLAKKWNDSTILLDSNQLDRMQQIGQSVETS
FEVETIHLFKIYPQQMPKYQKYRSFSLRG

Sequences:

>Translated_189_residues
MEILSHYFIGIDLPNHVKQNLIEVQQKLPSSFSYKQWTHKFDFHITLTFLGEIDDKQLTNVEQYLNNLEKKPFFLTLDQL
GVFGNQKQPRVLWAGLEHSNELHELHHQISHMLTQLELSKDNRPYRPHITLAKKWNDSTILLDSNQLDRMQQIGQSVETS
FEVETIHLFKIYPQQMPKYQKYRSFSLRG
>Mature_189_residues
MEILSHYFIGIDLPNHVKQNLIEVQQKLPSSFSYKQWTHKFDFHITLTFLGEIDDKQLTNVEQYLNNLEKKPFFLTLDQL
GVFGNQKQPRVLWAGLEHSNELHELHHQISHMLTQLELSKDNRPYRPHITLAKKWNDSTILLDSNQLDRMQQIGQSVETS
FEVETIHLFKIYPQQMPKYQKYRSFSLRG

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 22478; Mature: 22478

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEILSHYFIGIDLPNHVKQNLIEVQQKLPSSFSYKQWTHKFDFHITLTFLGEIDDKQLTN
CCCHHCEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHCEEEEEEEEEEEECCCCCHHHHH
VEQYLNNLEKKPFFLTLDQLGVFGNQKQPRVLWAGLEHSNELHELHHQISHMLTQLELSK
HHHHHHHHCCCCEEEEHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
DNRPYRPHITLAKKWNDSTILLDSNQLDRMQQIGQSVETSFEVETIHLFKIYPQQMPKYQ
CCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEEEEEECHHHCCHHH
KYRSFSLRG
HHCCCCCCC
>Mature Secondary Structure
MEILSHYFIGIDLPNHVKQNLIEVQQKLPSSFSYKQWTHKFDFHITLTFLGEIDDKQLTN
CCCHHCEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHCEEEEEEEEEEEECCCCCHHHHH
VEQYLNNLEKKPFFLTLDQLGVFGNQKQPRVLWAGLEHSNELHELHHQISHMLTQLELSK
HHHHHHHHCCCCEEEEHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
DNRPYRPHITLAKKWNDSTILLDSNQLDRMQQIGQSVETSFEVETIHLFKIYPQQMPKYQ
CCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEEEEEECHHHCCHHH
KYRSFSLRG
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8190087 [H]