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The map label for this gene is rsmE [H]

Identifier: 23099420

GI number: 23099420

Start: 1992304

End: 1993071

Strand: Reverse

Name: rsmE [H]

Synonym: OB1965

Alternate gene names: 23099420

Gene position: 1993071-1992304 (Counterclockwise)

Preceding gene: 23099421

Following gene: 23099419

Centisome position: 54.9

GC content: 35.42

Gene sequence:

>768_bases
ATGCAAAGATACTTTGTATCCCAAGAAAATGTCACTGAGAACTCCATACGAATCATTGGAGATGATGTCCATCATATTCA
ACGAGTAATGCGAGCACAAGTTGGAGAGAAATTAATTTGCGTTTCTGATAATGGACGAGCTGCGTTAACCTATATTATAA
AGTTATCCGATGACTATGTGGATGTGGAAATAGAAAAGTGGTTAGAGGAATCAAATGAATTACCAGTATCTGTTACGATT
GCTCAAGGTATTCCAAAAGGAGATAAGTTTGATTTGATCCTTCAAAAAGGGACTGAATTAGGCGCGGACGCGTTTATTCC
ATTCCAAGCTGAACGGTCAGTAGCTCTTTGGAATGATGAAAAAAAATATCATAAAAAAGTAAAGAGGTTTCAAAAAATCC
TTAAAGAAGCAAGTGAGCAAAGTCATCGTAATCGTATACCTTCGCTACACTCAGTAATGAATTTAAAACAAATTTTGGAT
GAAGCTTCAGCATACACAGTGAAATTATTTGCTTATGAAGAAGAAGCAAAAACAGAGAAGATGTCTTCAAACTTAGTGAA
CACACTACAAAACATCTTATCTCATGATAAAATAATTCTCTGTATTGGTCCTGAAGGCGGCTTTTCAATTAAAGAAGCAG
ATCAATTAAGGGAAAATGGATTTATACCCATTCGCCTTGGAAAACGAATTTTACGCACAGAAACAGCCGCTTTATATGCT
CTATCGAGTATTTCCTATCAATTAGAAGAATTGAGGTGTTTAACATGA

Upstream 100 bases:

>100_bases
ATAATTTAACAAAACAAGGATTTGAAGTTATATCTCTAAACGAGATGGAAGACTGGGTTTCGATTGTCGCTAAGAAGCCA
AGTGAAAAGTAGGTGAAACG

Downstream 100 bases:

>100_bases
CAACAGTGGCTTTTCACACGCTAGGATGTAAAGTAAATCATTATGAAACAGAAGGAATCTGGAGAATGTTTAAAGAAGAA
GGATATGAGAGAGTAGACTT

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MQRYFVSQENVTENSIRIIGDDVHHIQRVMRAQVGEKLICVSDNGRAALTYIIKLSDDYVDVEIEKWLEESNELPVSVTI
AQGIPKGDKFDLILQKGTELGADAFIPFQAERSVALWNDEKKYHKKVKRFQKILKEASEQSHRNRIPSLHSVMNLKQILD
EASAYTVKLFAYEEEAKTEKMSSNLVNTLQNILSHDKIILCIGPEGGFSIKEADQLRENGFIPIRLGKRILRTETAALYA
LSSISYQLEELRCLT

Sequences:

>Translated_255_residues
MQRYFVSQENVTENSIRIIGDDVHHIQRVMRAQVGEKLICVSDNGRAALTYIIKLSDDYVDVEIEKWLEESNELPVSVTI
AQGIPKGDKFDLILQKGTELGADAFIPFQAERSVALWNDEKKYHKKVKRFQKILKEASEQSHRNRIPSLHSVMNLKQILD
EASAYTVKLFAYEEEAKTEKMSSNLVNTLQNILSHDKIILCIGPEGGFSIKEADQLRENGFIPIRLGKRILRTETAALYA
LSSISYQLEELRCLT
>Mature_255_residues
MQRYFVSQENVTENSIRIIGDDVHHIQRVMRAQVGEKLICVSDNGRAALTYIIKLSDDYVDVEIEKWLEESNELPVSVTI
AQGIPKGDKFDLILQKGTELGADAFIPFQAERSVALWNDEKKYHKKVKRFQKILKEASEQSHRNRIPSLHSVMNLKQILD
EASAYTVKLFAYEEEAKTEKMSSNLVNTLQNILSHDKIILCIGPEGGFSIKEADQLRENGFIPIRLGKRILRTETAALYA
LSSISYQLEELRCLT

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=251, Percent_Identity=29.4820717131474, Blast_Score=105, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 29083; Mature: 29083

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRYFVSQENVTENSIRIIGDDVHHIQRVMRAQVGEKLICVSDNGRAALTYIIKLSDDYV
CCCEECCCCCCCCCCEEEECCHHHHHHHHHHHHHCCEEEEEECCCCEEEEEEEEECCCEE
DVEIEKWLEESNELPVSVTIAQGIPKGDKFDLILQKGTELGADAFIPFQAERSVALWNDE
EHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCEECCCCCEEEECCC
KKYHKKVKRFQKILKEASEQSHRNRIPSLHSVMNLKQILDEASAYTVKLFAYEEEAKTEK
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEEECHHHHHHH
MSSNLVNTLQNILSHDKIILCIGPEGGFSIKEADQLRENGFIPIRLGKRILRTETAALYA
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCEEEEHHHHHHHHHHHHHHHH
LSSISYQLEELRCLT
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MQRYFVSQENVTENSIRIIGDDVHHIQRVMRAQVGEKLICVSDNGRAALTYIIKLSDDYV
CCCEECCCCCCCCCCEEEECCHHHHHHHHHHHHHCCEEEEEECCCCEEEEEEEEECCCEE
DVEIEKWLEESNELPVSVTIAQGIPKGDKFDLILQKGTELGADAFIPFQAERSVALWNDE
EHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCEECCCCCEEEECCC
KKYHKKVKRFQKILKEASEQSHRNRIPSLHSVMNLKQILDEASAYTVKLFAYEEEAKTEK
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEEECHHHHHHH
MSSNLVNTLQNILSHDKIILCIGPEGGFSIKEADQLRENGFIPIRLGKRILRTETAALYA
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCEEEEHHHHHHHHHHHHHHHH
LSSISYQLEELRCLT
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]