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The map label for this gene is ldh [H]

Identifier: 23099324

GI number: 23099324

Start: 1900627

End: 1901721

Strand: Reverse

Name: ldh [H]

Synonym: OB1869

Alternate gene names: 23099324

Gene position: 1901721-1900627 (Counterclockwise)

Preceding gene: 23099325

Following gene: 23099323

Centisome position: 52.38

GC content: 36.62

Gene sequence:

>1095_bases
ATGGAAATCTTTACAAGCTTACAAAAATATGATTATGAACAATTACTTTTTTGCCAAGATAAAAATTCAGGATTAAAAGC
GATTATTGCTATACATGATACTACATTAGGACCCGCACTAGGCGGAACGAGAATGTGGACATATAAGACAGAAGATGATG
CAATAGTTGACGCCCTACGGTTGGCAAAAGGAATGACATATAAAAATGCAGCTGCTGGATTAAATCTTGGTGGAGGGAAA
ACAGTAATTATCGGTGATCCAAAAAAAGATAAAAATCCCGAAATGTTTCGTGCGTTTGGAAGATACATCCAAGGTTTAAA
CGGGCGGTATATTACTGCAGAAGATGTTGGAACAACTGAAATGGATATGGACTTAATACATTTAGAAACAGATTATGTTA
CAGGAACCTCTTCTTATTCAGGTGCATCCGGTAATCCATCTCCTATTACAGCGTTAGGATTATATCATGGTATGAAAGCA
ACGGCCAAAGAAGCTTTCGGAACAGAGTCGCTGGAAGGAAAAACCGTAGCAGTTCAAGGTGTTGGGAATGTTGCATATCA
TCTATGTGAATTTTTACATAAAGAAGGAGCAAATTTAATCGTTACTGATATTAATAAAGAGTCTGTAAAGCGTGCAGTAG
AAGATTTTGGGGCAAAAGCAGTAGACCCAGATGAGATTTATGGAGTGGAGTGCGATATTTATGCTCCGTGTGCACTAGGA
GCTACAATTAACGATGAAACGTTAGATAAATTGAAAGCAAAAGCTGTCGCAGGATCTGCTAACAACCAATTAATGTCAAC
AAAACATGGTGATATATTGCATGAACGGGGAATTGTTTACGCACCAGATTATGTTATTAATGCAGGTGGAGTTATTAATG
TTGCAGATGAGTTAGTTGGTTATAACAGAGAAAGAGCAATGAAAAAAGTAGAAATGGTTTATGACAATGTAGAGAAAGTG
TTCGAGATTTCAAAAAGAGATAATATTCCTACACATGTTGCAGCAGATAGAATGGCGGAGGAACGAATTCAATCCGTACG
TAATTCTAGAAGCCAGTTTCTACGAAATGAAAAACACATACTAAATTTTAATTAA

Upstream 100 bases:

>100_bases
ATTAGTTGGCATACAATTTGCAAGTTACTATTGAAAGGAGTGGTGTGGATTAAGTGCACCGTTTAGTGGTTCTGTGAATT
CATTCTTAGGAGGAATAATA

Downstream 100 bases:

>100_bases
GAAAGCCAAGTCGTTGGAGGTTGTCTTGTGCAAACAAATGGAAGGATACTAGTGGTTCAACCAGAATTATACGCAACAAC
TATTGGCGTGTTTGAAAATG

Product: leucine dehydrogenase

Products: NA

Alternate protein names: LeuDH [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MEIFTSLQKYDYEQLLFCQDKNSGLKAIIAIHDTTLGPALGGTRMWTYKTEDDAIVDALRLAKGMTYKNAAAGLNLGGGK
TVIIGDPKKDKNPEMFRAFGRYIQGLNGRYITAEDVGTTEMDMDLIHLETDYVTGTSSYSGASGNPSPITALGLYHGMKA
TAKEAFGTESLEGKTVAVQGVGNVAYHLCEFLHKEGANLIVTDINKESVKRAVEDFGAKAVDPDEIYGVECDIYAPCALG
ATINDETLDKLKAKAVAGSANNQLMSTKHGDILHERGIVYAPDYVINAGGVINVADELVGYNRERAMKKVEMVYDNVEKV
FEISKRDNIPTHVAADRMAEERIQSVRNSRSQFLRNEKHILNFN

Sequences:

>Translated_364_residues
MEIFTSLQKYDYEQLLFCQDKNSGLKAIIAIHDTTLGPALGGTRMWTYKTEDDAIVDALRLAKGMTYKNAAAGLNLGGGK
TVIIGDPKKDKNPEMFRAFGRYIQGLNGRYITAEDVGTTEMDMDLIHLETDYVTGTSSYSGASGNPSPITALGLYHGMKA
TAKEAFGTESLEGKTVAVQGVGNVAYHLCEFLHKEGANLIVTDINKESVKRAVEDFGAKAVDPDEIYGVECDIYAPCALG
ATINDETLDKLKAKAVAGSANNQLMSTKHGDILHERGIVYAPDYVINAGGVINVADELVGYNRERAMKKVEMVYDNVEKV
FEISKRDNIPTHVAADRMAEERIQSVRNSRSQFLRNEKHILNFN
>Mature_364_residues
MEIFTSLQKYDYEQLLFCQDKNSGLKAIIAIHDTTLGPALGGTRMWTYKTEDDAIVDALRLAKGMTYKNAAAGLNLGGGK
TVIIGDPKKDKNPEMFRAFGRYIQGLNGRYITAEDVGTTEMDMDLIHLETDYVTGTSSYSGASGNPSPITALGLYHGMKA
TAKEAFGTESLEGKTVAVQGVGNVAYHLCEFLHKEGANLIVTDINKESVKRAVEDFGAKAVDPDEIYGVECDIYAPCALG
ATINDETLDKLKAKAVAGSANNQLMSTKHGDILHERGIVYAPDYVINAGGVINVADELVGYNRERAMKKVEMVYDNVEKV
FEISKRDNIPTHVAADRMAEERIQSVRNSRSQFLRNEKHILNFN

Specific function: Functions catabolically in the bacterial metabolism of branched-chain L-amino acids, and plays an important role in spore germination in cooperation with alanine dehydrogenase [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=306, Percent_Identity=30.0653594771242, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI4885281, Length=306, Percent_Identity=29.7385620915033, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17544676, Length=311, Percent_Identity=27.6527331189711, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI45553475, Length=349, Percent_Identity=27.7936962750716, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI45549226, Length=306, Percent_Identity=29.4117647058824, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24649460, Length=271, Percent_Identity=29.8892988929889, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24649283, Length=274, Percent_Identity=27.007299270073, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR016211
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.9 [H]

Molecular weight: Translated: 39905; Mature: 39905

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIFTSLQKYDYEQLLFCQDKNSGLKAIIAIHDTTLGPALGGTRMWTYKTEDDAIVDALR
CCHHHHHHHCCHHHEEEEECCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHH
LAKGMTYKNAAAGLNLGGGKTVIIGDPKKDKNPEMFRAFGRYIQGLNGRYITAEDVGTTE
HHHCCCCCHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCC
MDMDLIHLETDYVTGTSSYSGASGNPSPITALGLYHGMKATAKEAFGTESLEGKTVAVQG
CCEEEEEEEECEEECCCCCCCCCCCCCCEEHHHHHHCCHHHHHHHCCCCCCCCCEEEEEC
VGNVAYHLCEFLHKEGANLIVTDINKESVKRAVEDFGAKAVDPDEIYGVECDIYAPCALG
CHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCCHHHEEEEEECEECCEECC
ATINDETLDKLKAKAVAGSANNQLMSTKHGDILHERGIVYAPDYVINAGGVINVADELVG
CCCCHHHHHHHHHHHHCCCCCCCEECCCCCCHHHHCCCEECCCEEEECCCEEEEHHHHHC
YNRERAMKKVEMVYDNVEKVFEISKRDNIPTHVAADRMAEERIQSVRNSRSQFLRNEKHI
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LNFN
ECCC
>Mature Secondary Structure
MEIFTSLQKYDYEQLLFCQDKNSGLKAIIAIHDTTLGPALGGTRMWTYKTEDDAIVDALR
CCHHHHHHHCCHHHEEEEECCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHH
LAKGMTYKNAAAGLNLGGGKTVIIGDPKKDKNPEMFRAFGRYIQGLNGRYITAEDVGTTE
HHHCCCCCHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCC
MDMDLIHLETDYVTGTSSYSGASGNPSPITALGLYHGMKATAKEAFGTESLEGKTVAVQG
CCEEEEEEEECEEECCCCCCCCCCCCCCEEHHHHHHCCHHHHHHHCCCCCCCCCEEEEEC
VGNVAYHLCEFLHKEGANLIVTDINKESVKRAVEDFGAKAVDPDEIYGVECDIYAPCALG
CHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCCHHHEEEEEECEECCEECC
ATINDETLDKLKAKAVAGSANNQLMSTKHGDILHERGIVYAPDYVINAGGVINVADELVG
CCCCHHHHHHHHHHHHCCCCCCCEECCCCCCHHHHCCCEECCCEEEECCCEEEEHHHHHC
YNRERAMKKVEMVYDNVEKVFEISKRDNIPTHVAADRMAEERIQSVRNSRSQFLRNEKHI
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LNFN
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3069133; 1400267 [H]