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The map label for this gene is buk

Identifier: 23099323

GI number: 23099323

Start: 1899523

End: 1900599

Strand: Reverse

Name: buk

Synonym: OB1868

Alternate gene names: 23099323

Gene position: 1900599-1899523 (Counterclockwise)

Preceding gene: 23099324

Following gene: 23099322

Centisome position: 52.35

GC content: 35.93

Gene sequence:

>1077_bases
GTGCAAACAAATGGAAGGATACTAGTGGTTCAACCAGAATTATACGCAACAACTATTGGCGTGTTTGAAAATGAAAGGTT
TCTGTATAAACAAACGATTCAACATAATGAAGAAGAGTTAAGTAAATTCACTCGAATCATGGACCAAGTTGCTTATCGAA
AACAAACCCTTCTGCATCAATTAGATCTTGATGGTATAAATATTTCAAAATTTAGTGCTGTATGCGGAAGAGGTGGATTA
CTTCAACCTATTACTGGTGGAACTTATGCTGTAAATGATAAAATGCTTTATGATTTGAAAACAGCTTCCTATGGAGAACA
TGTATCTAATTTAGGGGCGGTTATAGCTGATAGCATTGCTAAGGGATTAAACATTCCTGCATATATTGTTGATCCGCCAG
TAGTTGATGAAATGCAGTCAATAGCGCGGATATCGGGAATTCCCGAAATTGAAAGAAAGAGTATATTTCATGCTTTGAAC
CATAGACAAGCAGGAAGACTAGCTTCCAAGCGTTTAGGAAAAAATTACAATGAGCTGAATCTTATTGTAATTCATATCGC
ACGAGGTATAACTATTGGCGCACATAATAAAGGGAAAATAATTGATGTTACTAATGGGTTAGAAGGGGAAGGTCCTTTCA
CTATAGATCGTTCTGGTGGCATTCCAATTGCTAGGTTTCTAAGATATGGGATAGATAAATTTGATCAGATCGAGGATTGG
GAACAGAAATTAATAAAACAATCTGGCTTAAAAGGATATCTTCACACTGAAGATCCAAAGGTTATTCAATTGAATTTATT
AAAAAAAGACCCTTGGACAATGGAAATCATGCAAGCATTAGCATATCAAATTGCGAAAGAAATTGGGTCAATGAGTACAG
TTTTACATGGAGAAGTAGATGGTATAGTTTTCACGGGGAACGTAGAAGATGATGATTTTGTAATGAAGGAAGTAATCACA
AGAATAAATTGGATTGCAGATGTGATGGTTTTTCCAGGTAATAATGAGCTTGAAGCTATGAATGAAGGCGTTCTTCAAGT
GATTCGAGGAGAAATCCCCGTGAAAGTCTATCCGTAA

Upstream 100 bases:

>100_bases
ACGAATTCAATCCGTACGTAATTCTAGAAGCCAGTTTCTACGAAATGAAAAACACATACTAAATTTTAATTAAGAAAGCC
AAGTCGTTGGAGGTTGTCTT

Downstream 100 bases:

>100_bases
AAAGGAGGGACATTAATGGCAACGGAATACGATTTAGTCATACTTGGAGGAGGAACTGGTGGTTATGTTGCCGCCATTCG
TGCTTCACAATTAAAAATGA

Product: butyrate kinase

Products: NA

Alternate protein names: BK; Branched-chain carboxylic acid kinase

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MQTNGRILVVQPELYATTIGVFENERFLYKQTIQHNEEELSKFTRIMDQVAYRKQTLLHQLDLDGINISKFSAVCGRGGL
LQPITGGTYAVNDKMLYDLKTASYGEHVSNLGAVIADSIAKGLNIPAYIVDPPVVDEMQSIARISGIPEIERKSIFHALN
HRQAGRLASKRLGKNYNELNLIVIHIARGITIGAHNKGKIIDVTNGLEGEGPFTIDRSGGIPIARFLRYGIDKFDQIEDW
EQKLIKQSGLKGYLHTEDPKVIQLNLLKKDPWTMEIMQALAYQIAKEIGSMSTVLHGEVDGIVFTGNVEDDDFVMKEVIT
RINWIADVMVFPGNNELEAMNEGVLQVIRGEIPVKVYP

Sequences:

>Translated_358_residues
MQTNGRILVVQPELYATTIGVFENERFLYKQTIQHNEEELSKFTRIMDQVAYRKQTLLHQLDLDGINISKFSAVCGRGGL
LQPITGGTYAVNDKMLYDLKTASYGEHVSNLGAVIADSIAKGLNIPAYIVDPPVVDEMQSIARISGIPEIERKSIFHALN
HRQAGRLASKRLGKNYNELNLIVIHIARGITIGAHNKGKIIDVTNGLEGEGPFTIDRSGGIPIARFLRYGIDKFDQIEDW
EQKLIKQSGLKGYLHTEDPKVIQLNLLKKDPWTMEIMQALAYQIAKEIGSMSTVLHGEVDGIVFTGNVEDDDFVMKEVIT
RINWIADVMVFPGNNELEAMNEGVLQVIRGEIPVKVYP
>Mature_358_residues
MQTNGRILVVQPELYATTIGVFENERFLYKQTIQHNEEELSKFTRIMDQVAYRKQTLLHQLDLDGINISKFSAVCGRGGL
LQPITGGTYAVNDKMLYDLKTASYGEHVSNLGAVIADSIAKGLNIPAYIVDPPVVDEMQSIARISGIPEIERKSIFHALN
HRQAGRLASKRLGKNYNELNLIVIHIARGITIGAHNKGKIIDVTNGLEGEGPFTIDRSGGIPIARFLRYGIDKFDQIEDW
EQKLIKQSGLKGYLHTEDPKVIQLNLLKKDPWTMEIMQALAYQIAKEIGSMSTVLHGEVDGIVFTGNVEDDDFVMKEVIT
RINWIADVMVFPGNNELEAMNEGVLQVIRGEIPVKVYP

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=169, Percent_Identity=28.4023668639053, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): BUK_OCEIH (Q8CXE5)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692789.1
- ProteinModelPortal:   Q8CXE5
- SMR:   Q8CXE5
- GeneID:   1015045
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1868
- NMPDR:   fig|221109.1.peg.1867
- HOGENOM:   HBG449785
- OMA:   RSIFHAL
- ProtClustDB:   PRK03011
- BioCyc:   OIHE221109:OB1868-MONOMER
- BRENDA:   2.7.2.7
- GO:   GO:0005737
- HAMAP:   MF_00542
- InterPro:   IPR000890
- InterPro:   IPR011245
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF3
- PIRSF:   PIRSF036458
- PRINTS:   PR00471
- TIGRFAMs:   TIGR02707

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.7

Molecular weight: Translated: 40016; Mature: 40016

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTNGRILVVQPELYATTIGVFENERFLYKQTIQHNEEELSKFTRIMDQVAYRKQTLLHQ
CCCCCEEEEECCHHHEEEEEHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LDLDGINISKFSAVCGRGGLLQPITGGTYAVNDKMLYDLKTASYGEHVSNLGAVIADSIA
HCCCCCCHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHH
KGLNIPAYIVDPPVVDEMQSIARISGIPEIERKSIFHALNHRQAGRLASKRLGKNYNELN
HCCCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
LIVIHIARGITIGAHNKGKIIDVTNGLEGEGPFTIDRSGGIPIARFLRYGIDKFDQIEDW
EEEEEEECCEEEECCCCCCEEEECCCCCCCCCEEEECCCCCHHHHHHHHCCCHHHHHHHH
EQKLIKQSGLKGYLHTEDPKVIQLNLLKKDPWTMEIMQALAYQIAKEIGSMSTVLHGEVD
HHHHHHHCCCCCEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCC
GIVFTGNVEDDDFVMKEVITRINWIADVMVFPGNNELEAMNEGVLQVIRGEIPVKVYP
CEEEECCCCCHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCCCEEECC
>Mature Secondary Structure
MQTNGRILVVQPELYATTIGVFENERFLYKQTIQHNEEELSKFTRIMDQVAYRKQTLLHQ
CCCCCEEEEECCHHHEEEEEHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LDLDGINISKFSAVCGRGGLLQPITGGTYAVNDKMLYDLKTASYGEHVSNLGAVIADSIA
HCCCCCCHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHH
KGLNIPAYIVDPPVVDEMQSIARISGIPEIERKSIFHALNHRQAGRLASKRLGKNYNELN
HCCCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
LIVIHIARGITIGAHNKGKIIDVTNGLEGEGPFTIDRSGGIPIARFLRYGIDKFDQIEDW
EEEEEEECCEEEECCCCCCEEEECCCCCCCCCEEEECCCCCHHHHHHHHCCCHHHHHHHH
EQKLIKQSGLKGYLHTEDPKVIQLNLLKKDPWTMEIMQALAYQIAKEIGSMSTVLHGEVD
HHHHHHHCCCCCEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCC
GIVFTGNVEDDDFVMKEVITRINWIADVMVFPGNNELEAMNEGVLQVIRGEIPVKVYP
CEEEECCCCCHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376