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The map label for this gene is birA [H]

Identifier: 23099219

GI number: 23099219

Start: 1804553

End: 1805539

Strand: Reverse

Name: birA [H]

Synonym: OB1764

Alternate gene names: 23099219

Gene position: 1805539-1804553 (Counterclockwise)

Preceding gene: 23099220

Following gene: 23099218

Centisome position: 49.73

GC content: 34.65

Gene sequence:

>987_bases
ATGCAATCAACCAGATCCAAATTAATAGAAATATTATCTAGTAATCATGAAGATTATGCATCCGGACAACGCCTTTCTGA
ACTACTAAGTATTTCACGAAACGCTGTGTGGAAACACATGAAAGAATTAGAAAAAGATGGTTATGTAATTGAAGCTAAAC
CGAGAAAAGGATATCGCATTATTAAATCACCTAATAAAATTAGTACAAATACGATACGATGGGGCCTAGAAACGGAATGG
TTGGGTAATGAAATCATTCATAAAACATCTGTTGGTTCCACACAACTTGTCGGTCACGAAGCAGCACAGAACGGAGCGGC
ACATGGAACAATTATAATAGCGGATGAACAAACTGCCGGAAGAGGTAGGGTTCAAAAAAATTGGGACTCTAATGGTGATG
GAATATGGATGACAATAATTCTGCGTCCAAATATTCCACCAAATCGTGCATCGGAGCTAACGTTACTTACTGCTGTTGCT
ATTACAAAAGCTATAAAAAAAATAACAAATATTGATCCATTAATAAAATGGCCAAACGATATTTTAATTAAAGAGAAAAA
GTTATGTGGAATATTAACAGAAATGCAAGCGGAACAAGACCAGGTACAATATATTCTTATTGGAACTGGATTAAATGTAA
ATCAATCGAATCAACTATGGGATAAACAACTACAAAATATTGCTACGTCCTTATATCAAGAGACGGGACATACGTTTGAA
AAACATACTCTTTTACAGCAAATTTTACTGCAATTTGAACGTACGTATAATAATTATATGGAACATGGATTTCAACCTAT
TAAACAACAATGGGAAGCAAATGCTTTTAAGATTGGATCTGAAATCGAAATTACTACATTTCATGAACAATGGGTAGGCA
AATTTCTTGGAATTTCAGATGAAGGTGCTTTATTAGCGGAGTCTTCAGATGGAAGCCCTGTAAAAATATATTCTGCTGAC
ATCAAATGGTTTTCTCGACCGAAATAG

Upstream 100 bases:

>100_bases
CAAAAGGAAAGTGGATAAAAATCTTATTGGAAGAAGTTGAATATTTAGTAGTATCGAAGCAATTACCTAATGAGAAAAAA
ATAATAAAGGAATGGGTGTT

Downstream 100 bases:

>100_bases
TACGTTTGTTTCAACTTAATCTACTTAACTTGGCTTGGCAGTTGTGGAAGTTGAAGTATCGTAAAAGTAACATAAATCAT
AGTTGACGGTGTTAATAATC

Product: biotin [acetyl-CoA carboxylase] ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MQSTRSKLIEILSSNHEDYASGQRLSELLSISRNAVWKHMKELEKDGYVIEAKPRKGYRIIKSPNKISTNTIRWGLETEW
LGNEIIHKTSVGSTQLVGHEAAQNGAAHGTIIIADEQTAGRGRVQKNWDSNGDGIWMTIILRPNIPPNRASELTLLTAVA
ITKAIKKITNIDPLIKWPNDILIKEKKLCGILTEMQAEQDQVQYILIGTGLNVNQSNQLWDKQLQNIATSLYQETGHTFE
KHTLLQQILLQFERTYNNYMEHGFQPIKQQWEANAFKIGSEIEITTFHEQWVGKFLGISDEGALLAESSDGSPVKIYSAD
IKWFSRPK

Sequences:

>Translated_328_residues
MQSTRSKLIEILSSNHEDYASGQRLSELLSISRNAVWKHMKELEKDGYVIEAKPRKGYRIIKSPNKISTNTIRWGLETEW
LGNEIIHKTSVGSTQLVGHEAAQNGAAHGTIIIADEQTAGRGRVQKNWDSNGDGIWMTIILRPNIPPNRASELTLLTAVA
ITKAIKKITNIDPLIKWPNDILIKEKKLCGILTEMQAEQDQVQYILIGTGLNVNQSNQLWDKQLQNIATSLYQETGHTFE
KHTLLQQILLQFERTYNNYMEHGFQPIKQQWEANAFKIGSEIEITTFHEQWVGKFLGISDEGALLAESSDGSPVKIYSAD
IKWFSRPK
>Mature_328_residues
MQSTRSKLIEILSSNHEDYASGQRLSELLSISRNAVWKHMKELEKDGYVIEAKPRKGYRIIKSPNKISTNTIRWGLETEW
LGNEIIHKTSVGSTQLVGHEAAQNGAAHGTIIIADEQTAGRGRVQKNWDSNGDGIWMTIILRPNIPPNRASELTLLTAVA
ITKAIKKITNIDPLIKWPNDILIKEKKLCGILTEMQAEQDQVQYILIGTGLNVNQSNQLWDKQLQNIATSLYQETGHTFE
KHTLLQQILLQFERTYNNYMEHGFQPIKQQWEANAFKIGSEIEITTFHEQWVGKFLGISDEGALLAESSDGSPVKIYSAD
IKWFSRPK

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=303, Percent_Identity=26.0726072607261, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 37163; Mature: 37163

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS00268 CECROPIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSTRSKLIEILSSNHEDYASGQRLSELLSISRNAVWKHMKELEKDGYVIEAKPRKGYRI
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEE
IKSPNKISTNTIRWGLETEWLGNEIIHKTSVGSTQLVGHEAAQNGAAHGTIIIADEQTAG
ECCCCCCCCCEEEECCCHHHCCCHHHHHCCCCCHHHHCHHHHCCCCCCCEEEEECCCCCC
RGRVQKNWDSNGDGIWMTIILRPNIPPNRASELTLLTAVAITKAIKKITNIDPLIKWPND
CCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCH
ILIKEKKLCGILTEMQAEQDQVQYILIGTGLNVNQSNQLWDKQLQNIATSLYQETGHTFE
HEEEHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
KHTLLQQILLQFERTYNNYMEHGFQPIKQQWEANAFKIGSEIEITTFHEQWVGKFLGISD
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEECCCEEEEEEEHHHHHHHHCCCCC
EGALLAESSDGSPVKIYSADIKWFSRPK
CCCEEEECCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MQSTRSKLIEILSSNHEDYASGQRLSELLSISRNAVWKHMKELEKDGYVIEAKPRKGYRI
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEE
IKSPNKISTNTIRWGLETEWLGNEIIHKTSVGSTQLVGHEAAQNGAAHGTIIIADEQTAG
ECCCCCCCCCEEEECCCHHHCCCHHHHHCCCCCHHHHCHHHHCCCCCCCEEEEECCCCCC
RGRVQKNWDSNGDGIWMTIILRPNIPPNRASELTLLTAVAITKAIKKITNIDPLIKWPND
CCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCH
ILIKEKKLCGILTEMQAEQDQVQYILIGTGLNVNQSNQLWDKQLQNIATSLYQETGHTFE
HEEEHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
KHTLLQQILLQFERTYNNYMEHGFQPIKQQWEANAFKIGSEIEITTFHEQWVGKFLGISD
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEECCCEEEEEEEHHHHHHHHCCCCC
EGALLAESSDGSPVKIYSADIKWFSRPK
CCCEEEECCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]