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The map label for this gene is cca

Identifier: 23099220

GI number: 23099220

Start: 1805515

End: 1806717

Strand: Reverse

Name: cca

Synonym: OB1765

Alternate gene names: 23099220

Gene position: 1806717-1805515 (Counterclockwise)

Preceding gene: 23099221

Following gene: 23099219

Centisome position: 49.76

GC content: 34.0

Gene sequence:

>1203_bases
GTGATATCTGAACCTTTTTTAAAAGCTTCACCAATACTAACGACTTTAATTTCAAATGGCTATGAAGGGTATTTTGTAGG
TGGATGTGTTCGAGATCTTCTTTTACAAAAAGATATTCATGATATTGATATTGCCACTTCTGCTACTCCTAATGAAGTTA
TGCACCTTTTTAAAAAGGTGATACCTGTGGGTATTGAACATGGAACCGTTATTGTTCGACATGGGGGAGAATCGTATGAA
GTCACAACTTATAGACAAGATGGTGAATATACAGATCATCGTCGACCAAATGATGTTCGTTTTGTTACAAATTTAGCGGA
AGATTTACGACGGCGTGATTTTACGATTAATGCATTGGCGATGGATACAAGTGGACAGATAACAGATTTATTCCATGGTC
AAGACGACTTGAAAAAGAAATTAATTCGTACTGTCGGAAATGCAGAAGAACGTTTTACAGAAGATGCATTACGAATTCTT
CGTGCAGTTCGTTTTTCGAGTCAGCTGGGCTGTACAATACAAGAAGATACTTTAGAAGCGATGTTTGTAATTCGAAAACA
GATTCAACATCTTGCAGTAGAGAGAATAACCATTGAGCTTACAAAGCTATTTCAAGGACAACATGTTTCTAAGGCAATTC
AATATATTATTGATTTACAACTTGATGAGCAGCTTCCGATATTTCAATCAGGGAAAAGTGATATTTTTAAAGCTATGAAA
GAAAATATCACTCCACTTCATTCATTCTCAGAAGTGATTGCAGTGTTTCATCTTTTAGATCCAACGATTAAAATTCACGA
TTGGATTAAAAATTATAAATGCTCTAACCATGTTAAAAACGATACCATCCAACTTATTAACGCTTATCATTATTATCAGT
CTCATGGTATAAATAATTGGCTTCTCTATATTCTGCCAACAAAACTATGGGATGGCTTTATACGTCTTATCTACGTGATA
GATCGTACTACTATTGAACGAAAGCAATTAGAAGAAATAACTGCAACACTTCCAATCGATAAAAGATCACAATTACATCT
TGACGGAAATGATTTGATGAATTGGTTTCCACATCGTCCAAAAGGAAAGTGGATAAAAATCTTATTGGAAGAAGTTGAAT
ATTTAGTAGTATCGAAGCAATTACCTAATGAGAAAAAAATAATAAAGGAATGGGTGTTATGCAATCAACCAGATCCAAAT
TAA

Upstream 100 bases:

>100_bases
GCATGTAAGAGATTACTTTGCTTCGAAGACGATTGTAAATCAATATGAGGCCTTATATGATACACTTTTACAGGAAAACA
ATTAAGTTGGAGGTGGGAAT

Downstream 100 bases:

>100_bases
TAGAAATATTATCTAGTAATCATGAAGATTATGCATCCGGACAACGCCTTTCTGAACTACTAAGTATTTCACGAAACGCT
GTGTGGAAACACATGAAAGA

Product: poly(A)polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MISEPFLKASPILTTLISNGYEGYFVGGCVRDLLLQKDIHDIDIATSATPNEVMHLFKKVIPVGIEHGTVIVRHGGESYE
VTTYRQDGEYTDHRRPNDVRFVTNLAEDLRRRDFTINALAMDTSGQITDLFHGQDDLKKKLIRTVGNAEERFTEDALRIL
RAVRFSSQLGCTIQEDTLEAMFVIRKQIQHLAVERITIELTKLFQGQHVSKAIQYIIDLQLDEQLPIFQSGKSDIFKAMK
ENITPLHSFSEVIAVFHLLDPTIKIHDWIKNYKCSNHVKNDTIQLINAYHYYQSHGINNWLLYILPTKLWDGFIRLIYVI
DRTTIERKQLEEITATLPIDKRSQLHLDGNDLMNWFPHRPKGKWIKILLEEVEYLVVSKQLPNEKKIIKEWVLCNQPDPN

Sequences:

>Translated_400_residues
MISEPFLKASPILTTLISNGYEGYFVGGCVRDLLLQKDIHDIDIATSATPNEVMHLFKKVIPVGIEHGTVIVRHGGESYE
VTTYRQDGEYTDHRRPNDVRFVTNLAEDLRRRDFTINALAMDTSGQITDLFHGQDDLKKKLIRTVGNAEERFTEDALRIL
RAVRFSSQLGCTIQEDTLEAMFVIRKQIQHLAVERITIELTKLFQGQHVSKAIQYIIDLQLDEQLPIFQSGKSDIFKAMK
ENITPLHSFSEVIAVFHLLDPTIKIHDWIKNYKCSNHVKNDTIQLINAYHYYQSHGINNWLLYILPTKLWDGFIRLIYVI
DRTTIERKQLEEITATLPIDKRSQLHLDGNDLMNWFPHRPKGKWIKILLEEVEYLVVSKQLPNEKKIIKEWVLCNQPDPN
>Mature_400_residues
MISEPFLKASPILTTLISNGYEGYFVGGCVRDLLLQKDIHDIDIATSATPNEVMHLFKKVIPVGIEHGTVIVRHGGESYE
VTTYRQDGEYTDHRRPNDVRFVTNLAEDLRRRDFTINALAMDTSGQITDLFHGQDDLKKKLIRTVGNAEERFTEDALRIL
RAVRFSSQLGCTIQEDTLEAMFVIRKQIQHLAVERITIELTKLFQGQHVSKAIQYIIDLQLDEQLPIFQSGKSDIFKAMK
ENITPLHSFSEVIAVFHLLDPTIKIHDWIKNYKCSNHVKNDTIQLINAYHYYQSHGINNWLLYILPTKLWDGFIRLIYVI
DRTTIERKQLEEITATLPIDKRSQLHLDGNDLMNWFPHRPKGKWIKILLEEVEYLVVSKQLPNEKKIIKEWVLCNQPDPN

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=247, Percent_Identity=34.8178137651822, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI87081691, Length=240, Percent_Identity=35.4166666666667, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789436, Length=184, Percent_Identity=35.3260869565217, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71995920, Length=207, Percent_Identity=36.231884057971, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6321016, Length=266, Percent_Identity=28.1954887218045, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI281360112, Length=263, Percent_Identity=31.5589353612167, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI21357337, Length=263, Percent_Identity=31.5589353612167, Blast_Score=108, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_OCEIH (Q8EQD6)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692686.1
- ProteinModelPortal:   Q8EQD6
- SMR:   Q8EQD6
- GeneID:   1018259
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1765
- NMPDR:   fig|221109.1.peg.1766
- HOGENOM:   HBG635063
- OMA:   AVERITI
- BioCyc:   OIHE221109:OB1765-MONOMER
- BRENDA:   2.7.7.21
- BRENDA:   2.7.7.25
- HAMAP:   MF_01263
- InterPro:   IPR023068
- InterPro:   IPR002646

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 46400; Mature: 46400

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: BINDING 28-28 BINDING 31-31 BINDING 112-112 BINDING 155-155 BINDING 158-158 BINDING 161-161 BINDING 164-164

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISEPFLKASPILTTLISNGYEGYFVGGCVRDLLLQKDIHDIDIATSATPNEVMHLFKKV
CCCCCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
IPVGIEHGTVIVRHGGESYEVTTYRQDGEYTDHRRPNDVRFVTNLAEDLRRRDFTINALA
HCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEE
MDTSGQITDLFHGQDDLKKKLIRTVGNAEERFTEDALRILRAVRFSSQLGCTIQEDTLEA
ECCCCCCHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHH
MFVIRKQIQHLAVERITIELTKLFQGQHVSKAIQYIIDLQLDEQLPIFQSGKSDIFKAMK
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHH
ENITPLHSFSEVIAVFHLLDPTIKIHDWIKNYKCSNHVKNDTIQLINAYHYYQSHGINNW
HCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCE
LLYILPTKLWDGFIRLIYVIDRTTIERKQLEEITATLPIDKRSQLHLDGNDLMNWFPHRP
EEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHCCCCCC
KGKWIKILLEEVEYLVVSKQLPNEKKIIKEWVLCNQPDPN
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MISEPFLKASPILTTLISNGYEGYFVGGCVRDLLLQKDIHDIDIATSATPNEVMHLFKKV
CCCCCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
IPVGIEHGTVIVRHGGESYEVTTYRQDGEYTDHRRPNDVRFVTNLAEDLRRRDFTINALA
HCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEE
MDTSGQITDLFHGQDDLKKKLIRTVGNAEERFTEDALRILRAVRFSSQLGCTIQEDTLEA
ECCCCCCHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHH
MFVIRKQIQHLAVERITIELTKLFQGQHVSKAIQYIIDLQLDEQLPIFQSGKSDIFKAMK
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHH
ENITPLHSFSEVIAVFHLLDPTIKIHDWIKNYKCSNHVKNDTIQLINAYHYYQSHGINNW
HCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCE
LLYILPTKLWDGFIRLIYVIDRTTIERKQLEEITATLPIDKRSQLHLDGNDLMNWFPHRP
EEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHCCCCCC
KGKWIKILLEEVEYLVVSKQLPNEKKIIKEWVLCNQPDPN
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376