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The map label for this gene is hslU

Identifier: 23099005

GI number: 23099005

Start: 1593239

End: 1594636

Strand: Direct

Name: hslU

Synonym: OB1550

Alternate gene names: 23099005

Gene position: 1593239-1594636 (Clockwise)

Preceding gene: 23099004

Following gene: 23099006

Centisome position: 43.88

GC content: 36.98

Gene sequence:

>1398_bases
ATGGGAATGGATTATACCCCAAGGCAAATTGTTGAGCAGTTAGACCAGTTTATTGTTGGTCAAAAGCAAGCAAAAAAATC
AGTAGCGGTTGCTCTCCGTAATCGTTACCGGAGAATGAAGTTAGACGAAGGGTTCCGTGATGAAATTGTCCCTAAAAACA
TTCTCATGATTGGTCCAACCGGTGTAGGAAAAACAGAAATTGCTAGAAGACTTGCAAAATTGGTGGGAGCACCTTTTATA
AAAGTTGAAGCAACAAAATTTACTGAGGTTGGGTATGTTGGTAGAGATGTAGAATCCATGGTAAGAGATTTAGTTGAAAT
GGCAGTACGAATGGTAAAAGAAGAAAAAATGGCTGGAGTAATGAATGAAGCTGAGGAAGAAGCTAATAAAAGATTAGTAA
AACTATTAGTTCCAGAATCAAAAAAGAAACCAGCAATGAAAAATCCATTCGAAATGTTATTCCAATCAGATGGCTCCGAT
GGAGATGACGAAACGACGGAACAAGATTCGCATGATGAAATTCGTTCGAAACGAAAAAGAATTGCACATCAATTAGCTCT
CGGAGAGCTTGAGGATCACATAGTTTCTGTAGAGATTGATGAAGTTCCACCATCCATGTTTGATATGTTGCAAGGATCGG
GTATGGAGCAGATGGGAATGAATATGCAAGATGCTTTTGGTCAGTTTATGCCAAAGAAAAAGAAAAAAAGAAATTTACCA
GTTTCAGAAGCTCGTAAAGTGCTTACGCAACAAGAAGCTCAAAAGCTGGTAGATATGGATGAAGTAGCACAAGAAGCTAC
TAGTCGCGCAGAAGGATCGGGTATTATCTTTATTGATGAGATTGATAAAGTAGCTGGAAAGCAAGAGAATGCTGCAAATG
TGTCTAGAGAAGGTGTACAACGAGACATTTTGCCAATTGTTGAAGGTTCTACTGTTGTAACAAAACATGGAACTGTAAAA
ACAGATCATATGTTATTTGTTGCTGCTGGTGCTTTTCATATGTCAAAACCATCCGATCTAATACCTGAACTGCAAGGGAG
ATTTCCAATTCGAGTAGAACTTGAGAAATTAACGGTGGAGGATTTCAAACGAATTTTAACCGAACCTTCCAATGCTTTAA
TTAAGCAATATCAACTTATGCTGCAAACAGAAGGTATAAATGTTGAGTTTACTGACGAGGCTATAGAAAGGCTAGCAGAA
ATTGCTTATCAAGTAAATCAAAACACGGATAATATTGGTGCTCGACGATTACATACGATATTAGAAAAGCTATTAGAAGA
TTTATCGTATGAGGCACCAGAAATAACAATGGAAAAAGTTGAAATTACAGTTGGTTATGTCGATGATAAACTATCATCTA
TTGTTCAAGATAAAGATTTAAGCCAATTCATACTATAA

Upstream 100 bases:

>100_bases
ATATAGCCCGTTCGGCATTAGAAGTTGCAGGAGAAATATGTGTGTTCACTAATGATCAAATAACCTTAGAAGTACTTGAA
TAAAAGGAGGCTATCCATTA

Downstream 100 bases:

>100_bases
TTTTAAATATCGAATGGAGGAAATATCATGGATTTATTAAGTAGAGCAAGAAAAATCAATGCAATGTTACAAACAGCAAC
AGGAAAGTCAGTGAATTTTA

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MGMDYTPRQIVEQLDQFIVGQKQAKKSVAVALRNRYRRMKLDEGFRDEIVPKNILMIGPTGVGKTEIARRLAKLVGAPFI
KVEATKFTEVGYVGRDVESMVRDLVEMAVRMVKEEKMAGVMNEAEEEANKRLVKLLVPESKKKPAMKNPFEMLFQSDGSD
GDDETTEQDSHDEIRSKRKRIAHQLALGELEDHIVSVEIDEVPPSMFDMLQGSGMEQMGMNMQDAFGQFMPKKKKKRNLP
VSEARKVLTQQEAQKLVDMDEVAQEATSRAEGSGIIFIDEIDKVAGKQENAANVSREGVQRDILPIVEGSTVVTKHGTVK
TDHMLFVAAGAFHMSKPSDLIPELQGRFPIRVELEKLTVEDFKRILTEPSNALIKQYQLMLQTEGINVEFTDEAIERLAE
IAYQVNQNTDNIGARRLHTILEKLLEDLSYEAPEITMEKVEITVGYVDDKLSSIVQDKDLSQFIL

Sequences:

>Translated_465_residues
MGMDYTPRQIVEQLDQFIVGQKQAKKSVAVALRNRYRRMKLDEGFRDEIVPKNILMIGPTGVGKTEIARRLAKLVGAPFI
KVEATKFTEVGYVGRDVESMVRDLVEMAVRMVKEEKMAGVMNEAEEEANKRLVKLLVPESKKKPAMKNPFEMLFQSDGSD
GDDETTEQDSHDEIRSKRKRIAHQLALGELEDHIVSVEIDEVPPSMFDMLQGSGMEQMGMNMQDAFGQFMPKKKKKRNLP
VSEARKVLTQQEAQKLVDMDEVAQEATSRAEGSGIIFIDEIDKVAGKQENAANVSREGVQRDILPIVEGSTVVTKHGTVK
TDHMLFVAAGAFHMSKPSDLIPELQGRFPIRVELEKLTVEDFKRILTEPSNALIKQYQLMLQTEGINVEFTDEAIERLAE
IAYQVNQNTDNIGARRLHTILEKLLEDLSYEAPEITMEKVEITVGYVDDKLSSIVQDKDLSQFIL
>Mature_464_residues
GMDYTPRQIVEQLDQFIVGQKQAKKSVAVALRNRYRRMKLDEGFRDEIVPKNILMIGPTGVGKTEIARRLAKLVGAPFIK
VEATKFTEVGYVGRDVESMVRDLVEMAVRMVKEEKMAGVMNEAEEEANKRLVKLLVPESKKKPAMKNPFEMLFQSDGSDG
DDETTEQDSHDEIRSKRKRIAHQLALGELEDHIVSVEIDEVPPSMFDMLQGSGMEQMGMNMQDAFGQFMPKKKKKRNLPV
SEARKVLTQQEAQKLVDMDEVAQEATSRAEGSGIIFIDEIDKVAGKQENAANVSREGVQRDILPIVEGSTVVTKHGTVKT
DHMLFVAAGAFHMSKPSDLIPELQGRFPIRVELEKLTVEDFKRILTEPSNALIKQYQLMLQTEGINVEFTDEAIERLAEI
AYQVNQNTDNIGARRLHTILEKLLEDLSYEAPEITMEKVEITVGYVDDKLSSIVQDKDLSQFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Homo sapiens, GI7242140, Length=103, Percent_Identity=35.9223300970874, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1790366, Length=463, Percent_Identity=50.9719222462203, Blast_Score=467, Evalue=1e-133,
Organism=Escherichia coli, GI1786642, Length=226, Percent_Identity=33.1858407079646, Blast_Score=99, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71982908, Length=256, Percent_Identity=28.515625, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI71982905, Length=256, Percent_Identity=28.515625, Blast_Score=92, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6319704, Length=123, Percent_Identity=34.9593495934959, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648291, Length=242, Percent_Identity=29.3388429752066, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648289, Length=242, Percent_Identity=29.3388429752066, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_OCEIH (Q8CXH1)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692471.1
- ProteinModelPortal:   Q8CXH1
- SMR:   Q8CXH1
- GeneID:   1017836
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1550
- NMPDR:   fig|221109.1.peg.1552
- HOGENOM:   HBG745965
- OMA:   EASKFTE
- ProtClustDB:   PRK05201
- BioCyc:   OIHE221109:OB1550-MONOMER
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 52456; Mature: 52325

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: BINDING 19-19 BINDING 279-279 BINDING 343-343 BINDING 415-415

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMDYTPRQIVEQLDQFIVGQKQAKKSVAVALRNRYRRMKLDEGFRDEIVPKNILMIGPT
CCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCEEEECCC
GVGKTEIARRLAKLVGAPFIKVEATKFTEVGYVGRDVESMVRDLVEMAVRMVKEEKMAGV
CCCHHHHHHHHHHHHCCCCEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MNEAEEEANKRLVKLLVPESKKKPAMKNPFEMLFQSDGSDGDDETTEQDSHDEIRSKRKR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
IAHQLALGELEDHIVSVEIDEVPPSMFDMLQGSGMEQMGMNMQDAFGQFMPKKKKKRNLP
HHHHHHHHHHHHCEEEEEECCCCHHHHHHHCCCCHHHHCCCHHHHHHHHCCCHHHHCCCC
VSEARKVLTQQEAQKLVDMDEVAQEATSRAEGSGIIFIDEIDKVAGKQENAANVSREGVQ
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEECHHHHCCCCCCHHHHHHHHHH
RDILPIVEGSTVVTKHGTVKTDHMLFVAAGAFHMSKPSDLIPELQGRFPIRVELEKLTVE
HHHHHHCCCCEEEEECCCCEECCEEEEEECCHHCCCCHHHHHHHCCCCCEEEEHHHHHHH
DFKRILTEPSNALIKQYQLMLQTEGINVEFTDEAIERLAEIAYQVNQNTDNIGARRLHTI
HHHHHHCCCHHHHHHHHHHHHEECCCCEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LEKLLEDLSYEAPEITMEKVEITVGYVDDKLSSIVQDKDLSQFIL
HHHHHHHHCCCCCCEEEEEEEEEEEEHHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure 
GMDYTPRQIVEQLDQFIVGQKQAKKSVAVALRNRYRRMKLDEGFRDEIVPKNILMIGPT
CCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCEEEECCC
GVGKTEIARRLAKLVGAPFIKVEATKFTEVGYVGRDVESMVRDLVEMAVRMVKEEKMAGV
CCCHHHHHHHHHHHHCCCCEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MNEAEEEANKRLVKLLVPESKKKPAMKNPFEMLFQSDGSDGDDETTEQDSHDEIRSKRKR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
IAHQLALGELEDHIVSVEIDEVPPSMFDMLQGSGMEQMGMNMQDAFGQFMPKKKKKRNLP
HHHHHHHHHHHHCEEEEEECCCCHHHHHHHCCCCHHHHCCCHHHHHHHHCCCHHHHCCCC
VSEARKVLTQQEAQKLVDMDEVAQEATSRAEGSGIIFIDEIDKVAGKQENAANVSREGVQ
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEECHHHHCCCCCCHHHHHHHHHH
RDILPIVEGSTVVTKHGTVKTDHMLFVAAGAFHMSKPSDLIPELQGRFPIRVELEKLTVE
HHHHHHCCCCEEEEECCCCEECCEEEEEECCHHCCCCHHHHHHHCCCCCEEEEHHHHHHH
DFKRILTEPSNALIKQYQLMLQTEGINVEFTDEAIERLAEIAYQVNQNTDNIGARRLHTI
HHHHHHCCCHHHHHHHHHHHHEECCCCEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LEKLLEDLSYEAPEITMEKVEITVGYVDDKLSSIVQDKDLSQFIL
HHHHHHHHCCCCCCEEEEEEEEEEEEHHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376