Click here to switch to the map view.

The map label for this gene is opuE [H]

Identifier: 23098809

GI number: 23098809

Start: 1399323

End: 1400765

Strand: Reverse

Name: opuE [H]

Synonym: OB1354

Alternate gene names: 23098809

Gene position: 1400765-1399323 (Counterclockwise)

Preceding gene: 23098812

Following gene: 23098808

Centisome position: 38.58

GC content: 36.24

Gene sequence:

>1443_bases
ATGACTTGGTATATTAGTTATATAGGTTTATATTTTATTCTCATGTTATCCATGGGAATTTATTATTTTTTTAAAGTAAA
GACGTACGATGAATATTTAATTGGTGGTTGGAATACTAATTTCTGGCCAATTGTTGGTACAATTATTAGTACATGGTGTG
GAGCCGCTGTGTTTATAGGTTGGGTTGGCATGGGATTTACTGTCGGACTCTCAGGCTATTTTAAATTTGCTTTACCTGGG
ATTCTCTTCTGCCTCTTACTCATTGTAGCTTTTGCTGGCCCACTTAGACGTCAGCGACTGTACACTATCGCCGATTTATT
TGGAGAACGTTTTGGTGGGAAATCAGGAATTATCCCCTCCGTTTTATCAGCTTTCATATATTCCGTCCCTACATTAGCCC
TACAAATGATCGGAATGTCATCTATTTTTACAATTGCACTTGGAATAGATGTAAATACAGGTATTGCACTTTCATTCGTT
CTAATAGTGTCATTTACTATCTTAGGCGGATTACCAGCTACTATCATTACAGATGCAATTCAATCAATTATTGTTATCAT
TGGAATTATAGTTCTAGCTATCTCAAGCATAAATTTCGCTGGAGGATTTGAGAATATTATAGCGAATACACCAGCTGATT
ATATATCTCCCTTAGGAGCTGAAGGACTCGGGAGTGTGTTACTTTATGCATTATCAGTCGGTCCATTTTATATAATTTGG
CAATCGACTTGGCAACGAATCTTTGCTTCAAAAAGTGAACAAGTAGCGAAAAAAGCTGGAGTTACAGGATTCATTATTGC
TGGATTAATCTCCATATTACCTTATACCATCGGAGTAATAGCAAGACAGTTCGTACCAACAGATATGGATCCAGATCTAA
TCTTTTCATATGTAACTGCAGAATTACTGCATCCTGCAATAGGGGGTATTGTTTTTATAGGTTTACTAGCTGCTTTAATG
ACTGGTGCTACTTCATTTATTTTACAAGGCAGTTCAAATCTAACAAGGGATTTATATCAACGTCTAGTACGCCCGAACGC
TAACAATAAACAACTCATGTTCTCAGCAAGAATTTCCGTTATTATCATCTCATTACTGGGGCTTATTGCTGCATATTTTG
TTACTAATATCGAAACAGCATACCAATGGGCATTGCGTCTATCTGCAACTGTATTAGTATTCCCATTCCTTGCAGTTATG
TTCTGGAAAAGAGTCACTAAAAAAGGGATGATATGGAGCATGATAAGTGCACTTATCGCAACTCTGTCATGGCCATATTT
AGGTTTAAATATCGACCATACTATTTTCGGATTCAGTGTATCCATTTTAACATTAGTTATAATCAGCTTATTAACCAGAC
ATGATAATTCTGAACAAGTCAGAGCAGTTTATTGGAATGACCTTAATTCCGCAAACCCTTCAGAAACAAAATCTGAACAA
TAA

Upstream 100 bases:

>100_bases
TGCTTGAATTCTATTTCTCGGTTTGATTTAATAACAACATATGTTTTTCTTACTAGTCCAAAAGAAATGCAGATTAATTC
AATGAAGGAGAATTTCACAT

Downstream 100 bases:

>100_bases
AGGAGTAAACATTATTTCTATGGATACGATAATTACAAATGCGAAAATTTTTACGATGAATCAGGAGAATGACGTTGTTG
GGTCATTCTCTATTAAATCT

Product: sodium:proline symporter

Products: Na (I) [Cytoplasm]; pantothenate [Cytoplasm] [C]

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MTWYISYIGLYFILMLSMGIYYFFKVKTYDEYLIGGWNTNFWPIVGTIISTWCGAAVFIGWVGMGFTVGLSGYFKFALPG
ILFCLLLIVAFAGPLRRQRLYTIADLFGERFGGKSGIIPSVLSAFIYSVPTLALQMIGMSSIFTIALGIDVNTGIALSFV
LIVSFTILGGLPATIITDAIQSIIVIIGIIVLAISSINFAGGFENIIANTPADYISPLGAEGLGSVLLYALSVGPFYIIW
QSTWQRIFASKSEQVAKKAGVTGFIIAGLISILPYTIGVIARQFVPTDMDPDLIFSYVTAELLHPAIGGIVFIGLLAALM
TGATSFILQGSSNLTRDLYQRLVRPNANNKQLMFSARISVIIISLLGLIAAYFVTNIETAYQWALRLSATVLVFPFLAVM
FWKRVTKKGMIWSMISALIATLSWPYLGLNIDHTIFGFSVSILTLVIISLLTRHDNSEQVRAVYWNDLNSANPSETKSEQ

Sequences:

>Translated_480_residues
MTWYISYIGLYFILMLSMGIYYFFKVKTYDEYLIGGWNTNFWPIVGTIISTWCGAAVFIGWVGMGFTVGLSGYFKFALPG
ILFCLLLIVAFAGPLRRQRLYTIADLFGERFGGKSGIIPSVLSAFIYSVPTLALQMIGMSSIFTIALGIDVNTGIALSFV
LIVSFTILGGLPATIITDAIQSIIVIIGIIVLAISSINFAGGFENIIANTPADYISPLGAEGLGSVLLYALSVGPFYIIW
QSTWQRIFASKSEQVAKKAGVTGFIIAGLISILPYTIGVIARQFVPTDMDPDLIFSYVTAELLHPAIGGIVFIGLLAALM
TGATSFILQGSSNLTRDLYQRLVRPNANNKQLMFSARISVIIISLLGLIAAYFVTNIETAYQWALRLSATVLVFPFLAVM
FWKRVTKKGMIWSMISALIATLSWPYLGLNIDHTIFGFSVSILTLVIISLLTRHDNSEQVRAVYWNDLNSANPSETKSEQ
>Mature_479_residues
TWYISYIGLYFILMLSMGIYYFFKVKTYDEYLIGGWNTNFWPIVGTIISTWCGAAVFIGWVGMGFTVGLSGYFKFALPGI
LFCLLLIVAFAGPLRRQRLYTIADLFGERFGGKSGIIPSVLSAFIYSVPTLALQMIGMSSIFTIALGIDVNTGIALSFVL
IVSFTILGGLPATIITDAIQSIIVIIGIIVLAISSINFAGGFENIIANTPADYISPLGAEGLGSVLLYALSVGPFYIIWQ
STWQRIFASKSEQVAKKAGVTGFIIAGLISILPYTIGVIARQFVPTDMDPDLIFSYVTAELLHPAIGGIVFIGLLAALMT
GATSFILQGSSNLTRDLYQRLVRPNANNKQLMFSARISVIIISLLGLIAAYFVTNIETAYQWALRLSATVLVFPFLAVMF
WKRVTKKGMIWSMISALIATLSWPYLGLNIDHTIFGFSVSILTLVIISLLTRHDNSEQVRAVYWNDLNSANPSETKSEQ

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI17941285, Length=460, Percent_Identity=26.0869565217391, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI4507031, Length=553, Percent_Identity=22.7848101265823, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI110835708, Length=567, Percent_Identity=24.1622574955908, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI14140236, Length=469, Percent_Identity=22.6012793176972, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI310128183, Length=493, Percent_Identity=24.5436105476673, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI109659836, Length=469, Percent_Identity=23.454157782516, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI4507033, Length=406, Percent_Identity=23.1527093596059, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI11141885, Length=465, Percent_Identity=25.1612903225806, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI109659839, Length=485, Percent_Identity=22.8865979381443, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI206597483, Length=520, Percent_Identity=23.4615384615385, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI206597487, Length=450, Percent_Identity=23.5555555555556, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI87082237, Length=446, Percent_Identity=24.4394618834081, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1787251, Length=499, Percent_Identity=25.8517034068136, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790503, Length=490, Percent_Identity=23.8775510204082, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17539284, Length=441, Percent_Identity=24.0362811791383, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648033, Length=445, Percent_Identity=25.3932584269663, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21356865, Length=445, Percent_Identity=25.3932584269663, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 52566; Mature: 52435

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWYISYIGLYFILMLSMGIYYFFKVKTYDEYLIGGWNTNFWPIVGTIISTWCGAAVFIG
CEEEHHHHHHHHHHHHHHHHHHEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHH
WVGMGFTVGLSGYFKFALPGILFCLLLIVAFAGPLRRQRLYTIADLFGERFGGKSGIIPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHH
VLSAFIYSVPTLALQMIGMSSIFTIALGIDVNTGIALSFVLIVSFTILGGLPATIITDAI
HHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
QSIIVIIGIIVLAISSINFAGGFENIIANTPADYISPLGAEGLGSVLLYALSVGPFYIIW
HHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHCCCEEEEH
QSTWQRIFASKSEQVAKKAGVTGFIIAGLISILPYTIGVIARQFVPTDMDPDLIFSYVTA
HHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ELLHPAIGGIVFIGLLAALMTGATSFILQGSSNLTRDLYQRLVRPNANNKQLMFSARISV
HHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCHHHHHHHHHHHCCCCCCCEEEHHHHHHH
IIISLLGLIAAYFVTNIETAYQWALRLSATVLVFPFLAVMFWKRVTKKGMIWSMISALIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
TLSWPYLGLNIDHTIFGFSVSILTLVIISLLTRHDNSEQVRAVYWNDLNSANPSETKSEQ
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TWYISYIGLYFILMLSMGIYYFFKVKTYDEYLIGGWNTNFWPIVGTIISTWCGAAVFIG
EEEHHHHHHHHHHHHHHHHHHEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHH
WVGMGFTVGLSGYFKFALPGILFCLLLIVAFAGPLRRQRLYTIADLFGERFGGKSGIIPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHH
VLSAFIYSVPTLALQMIGMSSIFTIALGIDVNTGIALSFVLIVSFTILGGLPATIITDAI
HHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
QSIIVIIGIIVLAISSINFAGGFENIIANTPADYISPLGAEGLGSVLLYALSVGPFYIIW
HHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHCCCEEEEH
QSTWQRIFASKSEQVAKKAGVTGFIIAGLISILPYTIGVIARQFVPTDMDPDLIFSYVTA
HHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ELLHPAIGGIVFIGLLAALMTGATSFILQGSSNLTRDLYQRLVRPNANNKQLMFSARISV
HHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCHHHHHHHHHHHCCCCCCCEEEHHHHHHH
IIISLLGLIAAYFVTNIETAYQWALRLSATVLVFPFLAVMFWKRVTKKGMIWSMISALIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
TLSWPYLGLNIDHTIFGFSVSILTLVIISLLTRHDNSEQVRAVYWNDLNSANPSETKSEQ
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; pantothenate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + pantothenate [Periplasm] = Na (I) [Cytoplasm] + pantothenate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]