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The map label for this gene is cysK [H]

Identifier: 23098563

GI number: 23098563

Start: 1161091

End: 1162020

Strand: Direct

Name: cysK [H]

Synonym: OB1108

Alternate gene names: 23098563

Gene position: 1161091-1162020 (Clockwise)

Preceding gene: 23098562

Following gene: 23098564

Centisome position: 31.98

GC content: 36.56

Gene sequence:

>930_bases
TTGGCAATTTATGAATCGATACAACAATTGATTGGCAATACACCTTTATTAAGAATTAATGAGATGGACATTCCAAATAA
TGTTCATCTCTATGCAAAATTAGAATTTTTAAATCCTGGCGGAAGTGTAAAAGATCGTTTAGGACAATATTTAATTGATA
AAGCTTGGGAGCAAGGCTTTCTTCGAGAGGGAAGTACAATAATCGAACCAACAGCTGGAAATACTGGTATTGGTTTAGCT
TTAGCTGCTAGAACGAAAAAGTTGTCGGTTATTTTTTGTGTACCTGAGCAATTTAGTGTCGAAAAGCAACAAATCATGAG
AGCTTTAGGTGCAACTATCATTCATACTCCAAAAGAAAAAGGGATGATGGGTGCAATAGAAAAAGCTGAACAATTAGTTG
AAGAAATCCCAAATAGCTATTCTCCACAGCAGTTTTCAAATAAATTTAATCCTATGACCTATTATGAGACGATGGCACCG
GAAATTTGGAGTGATTTACAAGGAGATATTGATATCTTTGTAGCGGGTGCGGGATCGGGAGGTACTTTTACAGGTTGTGC
GAGTTATTTTAAGGAAAGAAAAAACTCGATTCATAATGTTATCGTTGAGCCCGTTGGTTCAATTTTAGGGGGAGGAGAAG
CTGGTCCACATTTAACCGAAGGAATTGGAATGGAATTTATCCCTGAGTACATGAATCAAAGCTTTATGGATGAGGTACAA
ACTATTTCGGATAAAGAAGCTTTTTCGATGGTAGAAGAACTTGCTAGAAAAGAAGGATTGTTAGTGGGTAGTTCTTCTGG
TTCAGCGATGGTAGCTGCTTTAAACAAAGCAAGAGAAGCTAGATCAGGAACGACAATTGTTACTATTCTTCCTGATGGAA
GTGATCGCTATTTAAGTAAACAGATTTATAATGTGTCAAAGCAATTGTAA

Upstream 100 bases:

>100_bases
CAAAAGAAATGTTAAATGAAAAAGCAAGCTGGTCAGAAGTATTTTAAAAAAATAAAATCCTCATTGTACTCTTTCTCTCC
AAGGTAGGAGTGACAATTCA

Downstream 100 bases:

>100_bases
AGGAGTTTGGAGAGATGAGAGATAAGACAAAAATGATACATGGTGGAATTACTTCAGATGAACGTACTGGAGCAGTAAAT
GTCCCGATATATCAAGTAAG

Product: cysteine synthase

Products: L-cysteine; acetate

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MAIYESIQQLIGNTPLLRINEMDIPNNVHLYAKLEFLNPGGSVKDRLGQYLIDKAWEQGFLREGSTIIEPTAGNTGIGLA
LAARTKKLSVIFCVPEQFSVEKQQIMRALGATIIHTPKEKGMMGAIEKAEQLVEEIPNSYSPQQFSNKFNPMTYYETMAP
EIWSDLQGDIDIFVAGAGSGGTFTGCASYFKERKNSIHNVIVEPVGSILGGGEAGPHLTEGIGMEFIPEYMNQSFMDEVQ
TISDKEAFSMVEELARKEGLLVGSSSGSAMVAALNKAREARSGTTIVTILPDGSDRYLSKQIYNVSKQL

Sequences:

>Translated_309_residues
MAIYESIQQLIGNTPLLRINEMDIPNNVHLYAKLEFLNPGGSVKDRLGQYLIDKAWEQGFLREGSTIIEPTAGNTGIGLA
LAARTKKLSVIFCVPEQFSVEKQQIMRALGATIIHTPKEKGMMGAIEKAEQLVEEIPNSYSPQQFSNKFNPMTYYETMAP
EIWSDLQGDIDIFVAGAGSGGTFTGCASYFKERKNSIHNVIVEPVGSILGGGEAGPHLTEGIGMEFIPEYMNQSFMDEVQ
TISDKEAFSMVEELARKEGLLVGSSSGSAMVAALNKAREARSGTTIVTILPDGSDRYLSKQIYNVSKQL
>Mature_308_residues
AIYESIQQLIGNTPLLRINEMDIPNNVHLYAKLEFLNPGGSVKDRLGQYLIDKAWEQGFLREGSTIIEPTAGNTGIGLAL
AARTKKLSVIFCVPEQFSVEKQQIMRALGATIIHTPKEKGMMGAIEKAEQLVEEIPNSYSPQQFSNKFNPMTYYETMAPE
IWSDLQGDIDIFVAGAGSGGTFTGCASYFKERKNSIHNVIVEPVGSILGGGEAGPHLTEGIGMEFIPEYMNQSFMDEVQT
ISDKEAFSMVEELARKEGLLVGSSSGSAMVAALNKAREARSGTTIVTILPDGSDRYLSKQIYNVSKQL

Specific function: Catalyzes the conversion of O-acetylserine and homocysteine to cystathionine [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=309, Percent_Identity=40.7766990291262, Blast_Score=218, Evalue=4e-57,
Organism=Homo sapiens, GI295821200, Length=309, Percent_Identity=40.7766990291262, Blast_Score=218, Evalue=4e-57,
Organism=Homo sapiens, GI4557415, Length=309, Percent_Identity=40.7766990291262, Blast_Score=218, Evalue=4e-57,
Organism=Escherichia coli, GI1788754, Length=312, Percent_Identity=41.3461538461538, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI2367138, Length=299, Percent_Identity=36.7892976588629, Blast_Score=182, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17535051, Length=304, Percent_Identity=37.828947368421, Blast_Score=216, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17562970, Length=297, Percent_Identity=37.7104377104377, Blast_Score=207, Evalue=6e-54,
Organism=Caenorhabditis elegans, GI115535073, Length=307, Percent_Identity=41.3680781758958, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI32566674, Length=291, Percent_Identity=38.4879725085911, Blast_Score=204, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI17561720, Length=297, Percent_Identity=37.7104377104377, Blast_Score=185, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17534315, Length=315, Percent_Identity=39.3650793650794, Blast_Score=178, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI25147552, Length=317, Percent_Identity=38.4858044164038, Blast_Score=170, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI32566672, Length=213, Percent_Identity=38.0281690140845, Blast_Score=150, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17561716, Length=118, Percent_Identity=35.5932203389831, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6321594, Length=319, Percent_Identity=37.3040752351097, Blast_Score=189, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6321449, Length=329, Percent_Identity=34.6504559270517, Blast_Score=148, Evalue=9e-37,
Organism=Drosophila melanogaster, GI24643623, Length=309, Percent_Identity=42.7184466019417, Blast_Score=212, Evalue=2e-55,
Organism=Drosophila melanogaster, GI20129101, Length=309, Percent_Identity=42.7184466019417, Blast_Score=212, Evalue=2e-55,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 2.5.1.47

Molecular weight: Translated: 33786; Mature: 33655

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIYESIQQLIGNTPLLRINEMDIPNNVHLYAKLEFLNPGGSVKDRLGQYLIDKAWEQGF
CHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
LREGSTIIEPTAGNTGIGLALAARTKKLSVIFCVPEQFSVEKQQIMRALGATIIHTPKEK
HHCCCEEEECCCCCCCCEEEEEECCCEEEEEEECCCHHCHHHHHHHHHHCHHEEECCHHC
GMMGAIEKAEQLVEEIPNSYSPQQFSNKFNPMTYYETMAPEIWSDLQGDIDIFVAGAGSG
CHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHCHHHHHHCCCCEEEEEEECCCC
GTFTGCASYFKERKNSIHNVIVEPVGSILGGGEAGPHLTEGIGMEFIPEYMNQSFMDEVQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHCHHHHHHHH
TISDKEAFSMVEELARKEGLLVGSSSGSAMVAALNKAREARSGTTIVTILPDGSDRYLSK
HCCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
QIYNVSKQL
HHHHHHHCC
>Mature Secondary Structure 
AIYESIQQLIGNTPLLRINEMDIPNNVHLYAKLEFLNPGGSVKDRLGQYLIDKAWEQGF
HHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
LREGSTIIEPTAGNTGIGLALAARTKKLSVIFCVPEQFSVEKQQIMRALGATIIHTPKEK
HHCCCEEEECCCCCCCCEEEEEECCCEEEEEEECCCHHCHHHHHHHHHHCHHEEECCHHC
GMMGAIEKAEQLVEEIPNSYSPQQFSNKFNPMTYYETMAPEIWSDLQGDIDIFVAGAGSG
CHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHCHHHHHHCCCCEEEEEEECCCC
GTFTGCASYFKERKNSIHNVIVEPVGSILGGGEAGPHLTEGIGMEFIPEYMNQSFMDEVQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHCHHHHHHHH
TISDKEAFSMVEELARKEGLLVGSSSGSAMVAALNKAREARSGTTIVTILPDGSDRYLSK
HCCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
QIYNVSKQL
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 44000 [C]

Specific activity: 1100

Km value (mM): 0.7 {L-Ser}} 0.12 {acetyl-CoA}} 3.6 {S2-}} 0.1 {S2-}} 20 {O-acetyl-L-Ser}} 7.1 {O-acetyl-L-Ser}} 5 {O-acetyl-L-Ser}} [C]

Substrates: O3-acetyl-L-serine; hydrogen sulfide

Specific reaction: O3-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate

General reaction: Elimination; C-O bond cleavage [C]

Inhibitor: acetyl-CoA; D-cycloserine; L-cysteine; NH2OH; semicarbazide [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377 [H]