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The map label for this gene is thlA [H]

Identifier: 23098144

GI number: 23098144

Start: 724951

End: 726135

Strand: Direct

Name: thlA [H]

Synonym: OB0689

Alternate gene names: 23098144

Gene position: 724951-726135 (Clockwise)

Preceding gene: 23098143

Following gene: 23098146

Centisome position: 19.97

GC content: 40.17

Gene sequence:

>1185_bases
GTGGAGGATCCGATCGTTATTGTTAGTGCAATTAGAACGCCAATTGGAGCATATGGCCAATCATTGAAAAACATATCATC
TGGTTTCCTAGCAAGCCATGTAGTGAAAGAATCATTGAGAAGAGTCAATCTTCCTGCTGATCATATAGATGAGGTTATTT
TAGGAGAAGTAAGACAATCAACAGAATCATCGAATATTGCTAGAGTCGCAGCACTAAGAGCTGGTATTCCTGAACAAGTT
ACTGCTTTCACGGTAAATAGATTATGTGCTTCTGGAATTCAAGCAGTGACATCCGGAGTGCAACAAATTCTAAGCAATCA
AGCTGATATTGTAGTAGCGGGGGGAGCAGAGAGTATGAGCCGATCTCCAATATATTTACGAAATACTAGATTTGGAGGGG
ATCGAACAACTATCGTAGATTCTAATTTAGAAGCAGGACAACAACCTCCAGAAATCTATGGTAAGAGCCTATCAATGGGA
ATTACCGCTGAGAATGTTGCTAGGAAATATAAAATATCAAGAGAAGATCAAGATGCATTTGCATTAGAAAGCCAGCACAG
AGCAAAAAAAGCCATCGAAACAGGACGATTTAAAAAAGAGATAGCCCCTATCGAAGTTAAGGAAAAGAAACAAACATCTG
TATTTGCAGTAGATGAATATCCTCGACTTGATACATCTTTAGAGAAACTAGCAAGTCTAAAACCGGTATTTGAAGCAGAC
GGTACGGTGACGGCAGGTAATGCTTGTGGCAGAAATGATGGGGCGTCAGCTCTGGTAATCATGAAAGAGTCACGGGCCAA
ACATCTTGGGTTGAAACCACTCGCAAGAATTATAGACTGGAGTACAGCAGGTGTGTCTCCTGAAATGATGGGGATTGGAC
CAGTACCCGCAGTCAATCAATTACTTAAACGAACGAATAAAACATTGCAAGACATAGATTTAATAGAATTAAATGAAGCA
TTTGCCTCTCAAAGTATCGCTGTCATCCGCCAACTAGGTTTGGATTATGAAAAAGTAAATGTTAATGGTGGTGCAATTGC
ATTAGGTCATCCGGTCGGTGCTACAGGAACACGGATAATTACGACATTAATCTATGAGTTAATTCGAAGAAAACAACAAT
ATGGCATAGCTACCTTGTGCGCTGGTGGCGGTCAAGGAATGGCAATATTAATTGAACAATGCTAA

Upstream 100 bases:

>100_bases
ACTCGGATTTATGAAGGAACATCGGAAATTCAGAAGAATATTATTGCAGGACAATTGAAAAAACACTATAGCAACAGATA
AGAAGCAGGGAGGTATAAAG

Downstream 100 bases:

>100_bases
GCTATTTTGCATTATCAGGATATGTTGATTGTATATGATTGAGAGATTTCTTAATTAAATGACGTAAAGTATCCAGATTA
ATATCACTGAGTTTATTAAT

Product: thiolase B

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MEDPIVIVSAIRTPIGAYGQSLKNISSGFLASHVVKESLRRVNLPADHIDEVILGEVRQSTESSNIARVAALRAGIPEQV
TAFTVNRLCASGIQAVTSGVQQILSNQADIVVAGGAESMSRSPIYLRNTRFGGDRTTIVDSNLEAGQQPPEIYGKSLSMG
ITAENVARKYKISREDQDAFALESQHRAKKAIETGRFKKEIAPIEVKEKKQTSVFAVDEYPRLDTSLEKLASLKPVFEAD
GTVTAGNACGRNDGASALVIMKESRAKHLGLKPLARIIDWSTAGVSPEMMGIGPVPAVNQLLKRTNKTLQDIDLIELNEA
FASQSIAVIRQLGLDYEKVNVNGGAIALGHPVGATGTRIITTLIYELIRRKQQYGIATLCAGGGQGMAILIEQC

Sequences:

>Translated_394_residues
MEDPIVIVSAIRTPIGAYGQSLKNISSGFLASHVVKESLRRVNLPADHIDEVILGEVRQSTESSNIARVAALRAGIPEQV
TAFTVNRLCASGIQAVTSGVQQILSNQADIVVAGGAESMSRSPIYLRNTRFGGDRTTIVDSNLEAGQQPPEIYGKSLSMG
ITAENVARKYKISREDQDAFALESQHRAKKAIETGRFKKEIAPIEVKEKKQTSVFAVDEYPRLDTSLEKLASLKPVFEAD
GTVTAGNACGRNDGASALVIMKESRAKHLGLKPLARIIDWSTAGVSPEMMGIGPVPAVNQLLKRTNKTLQDIDLIELNEA
FASQSIAVIRQLGLDYEKVNVNGGAIALGHPVGATGTRIITTLIYELIRRKQQYGIATLCAGGGQGMAILIEQC
>Mature_394_residues
MEDPIVIVSAIRTPIGAYGQSLKNISSGFLASHVVKESLRRVNLPADHIDEVILGEVRQSTESSNIARVAALRAGIPEQV
TAFTVNRLCASGIQAVTSGVQQILSNQADIVVAGGAESMSRSPIYLRNTRFGGDRTTIVDSNLEAGQQPPEIYGKSLSMG
ITAENVARKYKISREDQDAFALESQHRAKKAIETGRFKKEIAPIEVKEKKQTSVFAVDEYPRLDTSLEKLASLKPVFEAD
GTVTAGNACGRNDGASALVIMKESRAKHLGLKPLARIIDWSTAGVSPEMMGIGPVPAVNQLLKRTNKTLQDIDLIELNEA
FASQSIAVIRQLGLDYEKVNVNGGAIALGHPVGATGTRIITTLIYELIRRKQQYGIATLCAGGGQGMAILIEQC

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=392, Percent_Identity=46.9387755102041, Blast_Score=354, Evalue=7e-98,
Organism=Homo sapiens, GI148539872, Length=396, Percent_Identity=45.4545454545455, Blast_Score=338, Evalue=5e-93,
Organism=Homo sapiens, GI4557237, Length=397, Percent_Identity=40.3022670025189, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI4501853, Length=397, Percent_Identity=39.5465994962217, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI4504327, Length=423, Percent_Identity=34.9881796690307, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI194353979, Length=395, Percent_Identity=32.4050632911392, Blast_Score=168, Evalue=8e-42,
Organism=Escherichia coli, GI1788554, Length=391, Percent_Identity=49.1048593350384, Blast_Score=355, Evalue=2e-99,
Organism=Escherichia coli, GI87082165, Length=395, Percent_Identity=47.0886075949367, Blast_Score=353, Evalue=1e-98,
Organism=Escherichia coli, GI1787663, Length=396, Percent_Identity=45.4545454545455, Blast_Score=317, Evalue=8e-88,
Organism=Escherichia coli, GI48994986, Length=399, Percent_Identity=41.8546365914787, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1788683, Length=410, Percent_Identity=35.1219512195122, Blast_Score=213, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI133906874, Length=393, Percent_Identity=46.5648854961832, Blast_Score=344, Evalue=4e-95,
Organism=Caenorhabditis elegans, GI25147385, Length=390, Percent_Identity=36.1538461538462, Blast_Score=249, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI17535921, Length=396, Percent_Identity=38.3838383838384, Blast_Score=243, Evalue=9e-65,
Organism=Caenorhabditis elegans, GI17551802, Length=422, Percent_Identity=35.3080568720379, Blast_Score=233, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17535917, Length=393, Percent_Identity=29.5165394402036, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6322031, Length=393, Percent_Identity=38.9312977099237, Blast_Score=249, Evalue=5e-67,
Organism=Saccharomyces cerevisiae, GI6325229, Length=402, Percent_Identity=39.0547263681592, Blast_Score=246, Evalue=6e-66,
Organism=Drosophila melanogaster, GI24655093, Length=396, Percent_Identity=47.7272727272727, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI17648125, Length=393, Percent_Identity=47.5826972010178, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24640423, Length=396, Percent_Identity=38.8888888888889, Blast_Score=265, Evalue=3e-71,
Organism=Drosophila melanogaster, GI17137578, Length=427, Percent_Identity=35.5971896955504, Blast_Score=219, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 42318; Mature: 42318

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDPIVIVSAIRTPIGAYGQSLKNISSGFLASHVVKESLRRVNLPADHIDEVILGEVRQS
CCCCEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TESSNIARVAALRAGIPEQVTAFTVNRLCASGIQAVTSGVQQILSNQADIVVAGGAESMS
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHCC
RSPIYLRNTRFGGDRTTIVDSNLEAGQQPPEIYGKSLSMGITAENVARKYKISREDQDAF
CCCEEEEECCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHH
ALESQHRAKKAIETGRFKKEIAPIEVKEKKQTSVFAVDEYPRLDTSLEKLASLKPVFEAD
HHHHHHHHHHHHHHCCHHHHCCCCEECCCCCCEEEEECCCCCCCHHHHHHHHCCCHHCCC
GTVTAGNACGRNDGASALVIMKESRAKHLGLKPLARIIDWSTAGVSPEMMGIGPVPAVNQ
CCEEECCCCCCCCCCCEEEEEECCCHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
LLKRTNKTLQDIDLIELNEAFASQSIAVIRQLGLDYEKVNVNGGAIALGHPVGATGTRII
HHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCCCHHHHH
TTLIYELIRRKQQYGIATLCAGGGQGMAILIEQC
HHHHHHHHHHHHHCCEEEEECCCCCCEEEEEECC
>Mature Secondary Structure
MEDPIVIVSAIRTPIGAYGQSLKNISSGFLASHVVKESLRRVNLPADHIDEVILGEVRQS
CCCCEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TESSNIARVAALRAGIPEQVTAFTVNRLCASGIQAVTSGVQQILSNQADIVVAGGAESMS
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHCC
RSPIYLRNTRFGGDRTTIVDSNLEAGQQPPEIYGKSLSMGITAENVARKYKISREDQDAF
CCCEEEEECCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHH
ALESQHRAKKAIETGRFKKEIAPIEVKEKKQTSVFAVDEYPRLDTSLEKLASLKPVFEAD
HHHHHHHHHHHHHHCCHHHHCCCCEECCCCCCEEEEECCCCCCCHHHHHHHHCCCHHCCC
GTVTAGNACGRNDGASALVIMKESRAKHLGLKPLARIIDWSTAGVSPEMMGIGPVPAVNQ
CCEEECCCCCCCCCCCEEEEEECCCHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
LLKRTNKTLQDIDLIELNEAFASQSIAVIRQLGLDYEKVNVNGGAIALGHPVGATGTRII
HHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCCCHHHHH
TTLIYELIRRKQQYGIATLCAGGGQGMAILIEQC
HHHHHHHHHHHHHCCEEEEECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]