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The map label for this gene is yitJ [H]

Identifier: 23098146

GI number: 23098146

Start: 727207

End: 728256

Strand: Direct

Name: yitJ [H]

Synonym: OB0691

Alternate gene names: 23098146

Gene position: 727207-728256 (Clockwise)

Preceding gene: 23098144

Following gene: 23098149

Centisome position: 20.03

GC content: 39.43

Gene sequence:

>1050_bases
ATGAAGCGGAGTTTACAAAGACGTTTGCAAGAAGGCACGGTAATAGCAGGAGAAGGGTATTTATTTGAATTAGAGAGGAG
GGGGTACTTACAGGCAGGTTCGTTTGTACCAGAAGTAGCCCTTGAAAATCCGGATGCGTTAAAACAGACGTATCGTGATT
TTATGAACGCTGGTTCGGATGTTGTATTAGCATTTACATATAATGCACATCGAGAGAAAATGCGTATTATTGGTAAGGAA
CAATTACTAGAACCGTTGAACAGAAGTGCTATTCGTTTAGCGAAAGAAGTAGCCAAAGAACATCCACAGGAAGAAGCATT
AGTTGCAGGAAATATTTCTAATACAAATATCTTTGACCCTAATGATGAATCAAGCAAACATAAAGTGAGAGAGATGTTTG
CCGAAATGGCACAGTGGAGTAAAGAAGAAGACGTTGATTTTATAAATGGGGAAACGTTCTATTATCATGAAGAGGCAGAG
ATTGCTTTGGAAGAAATATTGAAAAAAGACTTGCCAGCTGTCATCACACTAGGATTAATGGGAGAGAACATCTTAAGAGA
TTGCTATACAGTAGAAGAATCGTGTAAGATATTATCGGAAAAAGGAGCCCTTGTTGTTGGCATGAATTGTTTCCGAGGTC
CAGATACAATGCAGCCTTATATCGCAAAAATTAGACAGCATGTAGACGGTTATGTAGGTGCATTACCTATTCCATATCGT
ACATCAAATGAGCATCCGACTTTTTTCAATTTACCTGATGGTGGGTGTAGCTGTCATCTCCCTACTGAGACCACTTTTCC
AACTGCATTAGATCCACTGTACCATAACCGTTATGAACTGGCAGCATGGGCTAAAGAAGCAAAAGAAATCGGTGTTAATT
ATATCGGATTATGCTGTGGGGCTTCTCCAGCTATGCTAAGAGCTGTTGCAGAGTCTGTTGGGAAAGAGACCGTTAATTCT
CCTTATTCTCCTGACATGGAGAAACATTTCTTGTTTGGTAAGGATAAAAGTTTAAAAGAGCATAACGTAGGTTATCGATT
CAAGGCATAA

Upstream 100 bases:

>100_bases
GGCTTACAAGCCTTAAAGATAAGAAGAGCTATGTATTTTAACCCTTCTTCTTACTTTTAGAAGAGGGGTTTTTTGATTTT
TAGAATAGGAGGAGATTATT

Downstream 100 bases:

>100_bases
TATCGTCATTTCTCTGGAGGATTTATTAGCGTATTGGAAAAATCAAAGAGGTTGATGGGTGTGGCAATTATGAGGGAGAC
AGATAAATCCTCTAACTTGA

Product: betaine-homocysteine methyltransferase

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MKRSLQRRLQEGTVIAGEGYLFELERRGYLQAGSFVPEVALENPDALKQTYRDFMNAGSDVVLAFTYNAHREKMRIIGKE
QLLEPLNRSAIRLAKEVAKEHPQEEALVAGNISNTNIFDPNDESSKHKVREMFAEMAQWSKEEDVDFINGETFYYHEEAE
IALEEILKKDLPAVITLGLMGENILRDCYTVEESCKILSEKGALVVGMNCFRGPDTMQPYIAKIRQHVDGYVGALPIPYR
TSNEHPTFFNLPDGGCSCHLPTETTFPTALDPLYHNRYELAAWAKEAKEIGVNYIGLCCGASPAMLRAVAESVGKETVNS
PYSPDMEKHFLFGKDKSLKEHNVGYRFKA

Sequences:

>Translated_349_residues
MKRSLQRRLQEGTVIAGEGYLFELERRGYLQAGSFVPEVALENPDALKQTYRDFMNAGSDVVLAFTYNAHREKMRIIGKE
QLLEPLNRSAIRLAKEVAKEHPQEEALVAGNISNTNIFDPNDESSKHKVREMFAEMAQWSKEEDVDFINGETFYYHEEAE
IALEEILKKDLPAVITLGLMGENILRDCYTVEESCKILSEKGALVVGMNCFRGPDTMQPYIAKIRQHVDGYVGALPIPYR
TSNEHPTFFNLPDGGCSCHLPTETTFPTALDPLYHNRYELAAWAKEAKEIGVNYIGLCCGASPAMLRAVAESVGKETVNS
PYSPDMEKHFLFGKDKSLKEHNVGYRFKA
>Mature_349_residues
MKRSLQRRLQEGTVIAGEGYLFELERRGYLQAGSFVPEVALENPDALKQTYRDFMNAGSDVVLAFTYNAHREKMRIIGKE
QLLEPLNRSAIRLAKEVAKEHPQEEALVAGNISNTNIFDPNDESSKHKVREMFAEMAQWSKEEDVDFINGETFYYHEEAE
IALEEILKKDLPAVITLGLMGENILRDCYTVEESCKILSEKGALVVGMNCFRGPDTMQPYIAKIRQHVDGYVGALPIPYR
TSNEHPTFFNLPDGGCSCHLPTETTFPTALDPLYHNRYELAAWAKEAKEIGVNYIGLCCGASPAMLRAVAESVGKETVNS
PYSPDMEKHFLFGKDKSLKEHNVGYRFKA

Specific function: Terminal step in the de novo biosynthesis of methionine. [C]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Homo sapiens, GI13162290, Length=337, Percent_Identity=33.2344213649852, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI157266337, Length=348, Percent_Identity=30.1724137931034, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI295821189, Length=192, Percent_Identity=31.25, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 39340; Mature: 39340

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSLQRRLQEGTVIAGEGYLFELERRGYLQAGSFVPEVALENPDALKQTYRDFMNAGSD
CCHHHHHHHHCCCEEECCCEEEEECCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCC
VVLAFTYNAHREKMRIIGKEQLLEPLNRSAIRLAKEVAKEHPQEEALVAGNISNTNIFDP
EEEEEEECCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCC
NDESSKHKVREMFAEMAQWSKEEDVDFINGETFYYHEEAEIALEEILKKDLPAVITLGLM
CCCHHHHHHHHHHHHHHHCCCCCCCCEECCCEEEEECHHHHHHHHHHHHCCCCEEEHHHH
GENILRDCYTVEESCKILSEKGALVVGMNCFRGPDTMQPYIAKIRQHVDGYVGALPIPYR
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHCHHHCCCCCCCC
TSNEHPTFFNLPDGGCSCHLPTETTFPTALDPLYHNRYELAAWAKEAKEIGVNYIGLCCG
CCCCCCCEEECCCCCCEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECC
ASPAMLRAVAESVGKETVNSPYSPDMEKHFLFGKDKSLKEHNVGYRFKA
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHEECCCCCCCHHCCCCCEECC
>Mature Secondary Structure
MKRSLQRRLQEGTVIAGEGYLFELERRGYLQAGSFVPEVALENPDALKQTYRDFMNAGSD
CCHHHHHHHHCCCEEECCCEEEEECCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCC
VVLAFTYNAHREKMRIIGKEQLLEPLNRSAIRLAKEVAKEHPQEEALVAGNISNTNIFDP
EEEEEEECCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCC
NDESSKHKVREMFAEMAQWSKEEDVDFINGETFYYHEEAEIALEEILKKDLPAVITLGLM
CCCHHHHHHHHHHHHHHHCCCCCCCCEECCCEEEEECHHHHHHHHHHHHCCCCEEEHHHH
GENILRDCYTVEESCKILSEKGALVVGMNCFRGPDTMQPYIAKIRQHVDGYVGALPIPYR
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHCHHHCCCCCCCC
TSNEHPTFFNLPDGGCSCHLPTETTFPTALDPLYHNRYELAAWAKEAKEIGVNYIGLCCG
CCCCCCCEEECCCCCCEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECC
ASPAMLRAVAESVGKETVNSPYSPDMEKHFLFGKDKSLKEHNVGYRFKA
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHEECCCCCCCHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]