Click here to switch to the map view.

The map label for this gene is cssS [H]

Identifier: 23098040

GI number: 23098040

Start: 611239

End: 612621

Strand: Direct

Name: cssS [H]

Synonym: OB0585

Alternate gene names: 23098040

Gene position: 611239-612621 (Clockwise)

Preceding gene: 23098039

Following gene: 23098041

Centisome position: 16.84

GC content: 35.5

Gene sequence:

>1383_bases
ATGAAGCTTCAGTCGCAATTGAACGCAGCTTTCACAGCGTTGCTTTTAGTTATTATGACCACGGCGGGCTATATCATTTA
TTCGTTGATTTTGGATTTACTCATTCAAGACGAAAAAAGACAGCTAGCAGAAACTGGGGAGCTATTGGTTAGTTTTCTTA
ATGAACAATATAATAACGATGTAAATAATTATCAAAATATAAATGATTTCTTACAAGAACAGGACTTACAACTATTTTTA
TATGATCGAAATTTAGATACCGTATTGTTTTCGACAATGAATATTTCGGTCGTAAATGAATTTTTTCAACAAAATAATTT
TGCGAATAATGATGAGTTTTTATGGCAATATGGTAGTGATCGCTTTGTCACGTCACGTATCATTTTCTCTCCGGAGCAAA
CTGGATTGGAACTTATCTTGTTGACTCCGATGCATGATTTACAAGTAGTCCAACAAAATTTCTTTGTTCGACTTCTCTTG
GTATTTCTTGTTGGGGCGGTAGCTGCTGTTATACTTAGTTATTTTATGACAAATAAGTTAGTGACACCATTGTCTCAATT
AAAAACGGAGCTAAAAAAGGTAGAAAATCGTCAGTTTGATCGGATGAGCAGGGTGAAGGCAACTGGTGAAATTAAAGAAG
TAGAGCAAAGTGTATATGAAATGGCTCAAACATTGGAGCAATATATGAAATCACAACAAACTTTCTTCCAAAATGCCAGT
CATGAGTTAAAAACACCATTAATGACGATACAAGGGTATGCTGAAGGTATAAGAGATGGGATTTTTGATGAAAAAGATAC
AGAAAAAGGATTAGAGGTAATGGTTACGGAGGTTAATCGTTTGAAAGGGATTATCAATGAAATGATTCTGCTAGCAAAAT
TAGATACAGATCCTGCAGAATATTCACCTTTTCCACTTGAAGCAGATCAATTTATACATCAAGTAGTTGATCGAGCACAT
CCTATAGTCAATGAAGAAGGGATAGAATTAAACCATCAAGTGGAGAACGGGGTTATCCTATTTGCTGACGAAGAGAAATT
ACTTCGTGCTCTACTTAATATAGTTTTCAATGGAATTCGTCATTCTAAATCGACGGTAAATATTCAAGTTTATACCAAGG
ATCATAAGGTAATGATTGATGTAGAAGACGACGGAAGCGGTATTACAGAAGAAGATGCAGAGCATATTTTCCATCGGTTT
GTAAAAGGAAAGGATGGCGAGACAGGCCTTGGTCTTGCAATAGCTCGTGCAATTATCGAACAAGCAGGTGGTAATATATC
TGTATCCAATTCTGAAATTGGCGGAGCGAAATTTACGATTCAATTGCCGAAATATCAGTCAACAAATCATAATAGACAAA
AACCAAAGCAACTTCCAAAGTAG

Upstream 100 bases:

>100_bases
TTGATGATTTAATTAAACGAATTCGTAAAAAAATGGGCAATATCCCACTAGAAACCGTATGGGGTTTTGGATATCGCTTA
AGAAAAGATGAGGAATAACA

Downstream 100 bases:

>100_bases
TCTGGATTTTTGCAAAAATACCTATGCATTAAGATGGAGGAAATGAAAATTTGAGGATTAGCAGCTCATAATTGTTCGAT
CTCATTCAAAGGCCTTTATG

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MKLQSQLNAAFTALLLVIMTTAGYIIYSLILDLLIQDEKRQLAETGELLVSFLNEQYNNDVNNYQNINDFLQEQDLQLFL
YDRNLDTVLFSTMNISVVNEFFQQNNFANNDEFLWQYGSDRFVTSRIIFSPEQTGLELILLTPMHDLQVVQQNFFVRLLL
VFLVGAVAAVILSYFMTNKLVTPLSQLKTELKKVENRQFDRMSRVKATGEIKEVEQSVYEMAQTLEQYMKSQQTFFQNAS
HELKTPLMTIQGYAEGIRDGIFDEKDTEKGLEVMVTEVNRLKGIINEMILLAKLDTDPAEYSPFPLEADQFIHQVVDRAH
PIVNEEGIELNHQVENGVILFADEEKLLRALLNIVFNGIRHSKSTVNIQVYTKDHKVMIDVEDDGSGITEEDAEHIFHRF
VKGKDGETGLGLAIARAIIEQAGGNISVSNSEIGGAKFTIQLPKYQSTNHNRQKPKQLPK

Sequences:

>Translated_460_residues
MKLQSQLNAAFTALLLVIMTTAGYIIYSLILDLLIQDEKRQLAETGELLVSFLNEQYNNDVNNYQNINDFLQEQDLQLFL
YDRNLDTVLFSTMNISVVNEFFQQNNFANNDEFLWQYGSDRFVTSRIIFSPEQTGLELILLTPMHDLQVVQQNFFVRLLL
VFLVGAVAAVILSYFMTNKLVTPLSQLKTELKKVENRQFDRMSRVKATGEIKEVEQSVYEMAQTLEQYMKSQQTFFQNAS
HELKTPLMTIQGYAEGIRDGIFDEKDTEKGLEVMVTEVNRLKGIINEMILLAKLDTDPAEYSPFPLEADQFIHQVVDRAH
PIVNEEGIELNHQVENGVILFADEEKLLRALLNIVFNGIRHSKSTVNIQVYTKDHKVMIDVEDDGSGITEEDAEHIFHRF
VKGKDGETGLGLAIARAIIEQAGGNISVSNSEIGGAKFTIQLPKYQSTNHNRQKPKQLPK
>Mature_460_residues
MKLQSQLNAAFTALLLVIMTTAGYIIYSLILDLLIQDEKRQLAETGELLVSFLNEQYNNDVNNYQNINDFLQEQDLQLFL
YDRNLDTVLFSTMNISVVNEFFQQNNFANNDEFLWQYGSDRFVTSRIIFSPEQTGLELILLTPMHDLQVVQQNFFVRLLL
VFLVGAVAAVILSYFMTNKLVTPLSQLKTELKKVENRQFDRMSRVKATGEIKEVEQSVYEMAQTLEQYMKSQQTFFQNAS
HELKTPLMTIQGYAEGIRDGIFDEKDTEKGLEVMVTEVNRLKGIINEMILLAKLDTDPAEYSPFPLEADQFIHQVVDRAH
PIVNEEGIELNHQVENGVILFADEEKLLRALLNIVFNGIRHSKSTVNIQVYTKDHKVMIDVEDDGSGITEEDAEHIFHRF
VKGKDGETGLGLAIARAIIEQAGGNISVSNSEIGGAKFTIQLPKYQSTNHNRQKPKQLPK

Specific function: Member of the two-component regulatory system CssS/CssR required to control the cellular response to secretion stress. Required for the transcription of htrA. Could detect misfolded proteins at the membrane-cell wall interface and then activate CssR by ph

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790346, Length=240, Percent_Identity=31.6666666666667, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788393, Length=274, Percent_Identity=25.5474452554745, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1786600, Length=269, Percent_Identity=25.2788104089219, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI145693157, Length=259, Percent_Identity=28.1853281853282, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787894, Length=234, Percent_Identity=26.0683760683761, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786783, Length=296, Percent_Identity=23.9864864864865, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790436, Length=225, Percent_Identity=28, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789808, Length=297, Percent_Identity=25.5892255892256, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1789149, Length=351, Percent_Identity=23.3618233618234, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI48994928, Length=236, Percent_Identity=25.4237288135593, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI87081816, Length=259, Percent_Identity=24.3243243243243, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790300, Length=239, Percent_Identity=25.5230125523013, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788549, Length=220, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI87082128, Length=314, Percent_Identity=22.2929936305732, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787374, Length=302, Percent_Identity=21.1920529801325, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1786912, Length=233, Percent_Identity=26.1802575107296, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788279, Length=306, Percent_Identity=22.8758169934641, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788713, Length=226, Percent_Identity=22.1238938053097, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790551, Length=296, Percent_Identity=24.6621621621622, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789403, Length=290, Percent_Identity=25.1724137931034, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52454; Mature: 52454

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQSQLNAAFTALLLVIMTTAGYIIYSLILDLLIQDEKRQLAETGELLVSFLNEQYNND
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VNNYQNINDFLQEQDLQLFLYDRNLDTVLFSTMNISVVNEFFQQNNFANNDEFLWQYGSD
CHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
RFVTSRIIFSPEQTGLELILLTPMHDLQVVQQNFFVRLLLVFLVGAVAAVILSYFMTNKL
CEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTPLSQLKTELKKVENRQFDRMSRVKATGEIKEVEQSVYEMAQTLEQYMKSQQTFFQNAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HELKTPLMTIQGYAEGIRDGIFDEKDTEKGLEVMVTEVNRLKGIINEMILLAKLDTDPAE
HHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
YSPFPLEADQFIHQVVDRAHPIVNEEGIELNHQVENGVILFADEEKLLRALLNIVFNGIR
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEECCCCEEEEECHHHHHHHHHHHHHHHHH
HSKSTVNIQVYTKDHKVMIDVEDDGSGITEEDAEHIFHRFVKGKDGETGLGLAIARAIIE
CCCCEEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
QAGGNISVSNSEIGGAKFTIQLPKYQSTNHNRQKPKQLPK
HCCCCEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKLQSQLNAAFTALLLVIMTTAGYIIYSLILDLLIQDEKRQLAETGELLVSFLNEQYNND
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VNNYQNINDFLQEQDLQLFLYDRNLDTVLFSTMNISVVNEFFQQNNFANNDEFLWQYGSD
CHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
RFVTSRIIFSPEQTGLELILLTPMHDLQVVQQNFFVRLLLVFLVGAVAAVILSYFMTNKL
CEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTPLSQLKTELKKVENRQFDRMSRVKATGEIKEVEQSVYEMAQTLEQYMKSQQTFFQNAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HELKTPLMTIQGYAEGIRDGIFDEKDTEKGLEVMVTEVNRLKGIINEMILLAKLDTDPAE
HHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
YSPFPLEADQFIHQVVDRAHPIVNEEGIELNHQVENGVILFADEEKLLRALLNIVFNGIR
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEECCCCEEEEECHHHHHHHHHHHHHHHHH
HSKSTVNIQVYTKDHKVMIDVEDDGSGITEEDAEHIFHRFVKGKDGETGLGLAIARAIIE
CCCCEEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
QAGGNISVSNSEIGGAKFTIQLPKYQSTNHNRQKPKQLPK
HCCCCEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353931; 9639930; 9384377; 11555295 [H]