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The map label for this gene is ykwC [H]

Identifier: 23098036

GI number: 23098036

Start: 606934

End: 607830

Strand: Reverse

Name: ykwC [H]

Synonym: OB0581

Alternate gene names: 23098036

Gene position: 607830-606934 (Counterclockwise)

Preceding gene: 23098038

Following gene: 23098033

Centisome position: 16.74

GC content: 38.35

Gene sequence:

>897_bases
ATGTTATCAGCTAACACAACTACGATAGGTTTCATAGGTACTGGAGTTATGGGGAAAAGCATGGCAAAAAATCTGCTCAA
TGCTGGATATAGTATACGAATATTTACAAGAACAAAATCCAAAGCTGATGATTTATTAAATATAGGGGCAAAATGGGATA
AAGATATACCGACATTGGCAAAACAATGCGATGTAATTATTACAATGGTAGGTTATCCCTCTGATGTAGAAGAAGTATAC
TTAGGTGAGCAAGGTTTAATAAAAAACTGCAATAAAGGTTCCTACCTAATTGATATGACTACTTCCAAGCCATCATTAGC
TGAAGAAATCTTTTCGGTCAGTGAAAAACATGGGTTGCACGTATTAGATGCACCTGTTTCAGGGGGAGATATTGGAGCGG
AGAGTGGAACACTTGCTATAATGACAGGCGGAGAAAGAAGCGTGTATGATTACGTCCTCCCTATTTTTCAAGTTCTTGGG
GAGAATATATCTTATCAAGGCCCAGCAGGAACTGGCCAGCATACTAAAATGTCCAATCAAATTGCGATTGCATCCAACAT
GATTGGGGTTTGTGAGTCCTTATTATATGCAAAAAAAGCAGGACTTGATCCGAAAAAAGTATTGGCGACTATTTCCACTG
GTGCTGCTGCCAGTTTTTCTCTTTCAAAATTAGGTGCAAGAATGCTTGAAAATGACTTTAATCCCGGATTCTATGTAAAA
CATTTTATTAAAGACATGAGAATTGCTCTTGAATCTTCTCAAGCGTTCGGATTGGATACTCCTGGCTTAAAATTAGCTAT
TGATTTATATGAACAACTCTCAGATATGGGTGAGCAGGATAGTGGAACTCAAGCGTTAATCAAAGCCCTCGCATACAGGT
CCGAGATGACATTATAG

Upstream 100 bases:

>100_bases
TGACAAGACTATTTAATTCTTTACTTTTTGTTAAGTAAAGAAGGATTCCCTGTTTTTTTGTAGAATGGTTGTATTAAGTC
TTAAAATAAGGAGAATGTAA

Downstream 100 bases:

>100_bases
ATGAGTATTGGCAAATTATAATTATATCGAGTAGTGGTCAAGCCAACTTATCTAAAACTTTTCAGCCAAGAAATGAGGCC
GTTATTTCTGCTTCATTTCT

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MLSANTTTIGFIGTGVMGKSMAKNLLNAGYSIRIFTRTKSKADDLLNIGAKWDKDIPTLAKQCDVIITMVGYPSDVEEVY
LGEQGLIKNCNKGSYLIDMTTSKPSLAEEIFSVSEKHGLHVLDAPVSGGDIGAESGTLAIMTGGERSVYDYVLPIFQVLG
ENISYQGPAGTGQHTKMSNQIAIASNMIGVCESLLYAKKAGLDPKKVLATISTGAAASFSLSKLGARMLENDFNPGFYVK
HFIKDMRIALESSQAFGLDTPGLKLAIDLYEQLSDMGEQDSGTQALIKALAYRSEMTL

Sequences:

>Translated_298_residues
MLSANTTTIGFIGTGVMGKSMAKNLLNAGYSIRIFTRTKSKADDLLNIGAKWDKDIPTLAKQCDVIITMVGYPSDVEEVY
LGEQGLIKNCNKGSYLIDMTTSKPSLAEEIFSVSEKHGLHVLDAPVSGGDIGAESGTLAIMTGGERSVYDYVLPIFQVLG
ENISYQGPAGTGQHTKMSNQIAIASNMIGVCESLLYAKKAGLDPKKVLATISTGAAASFSLSKLGARMLENDFNPGFYVK
HFIKDMRIALESSQAFGLDTPGLKLAIDLYEQLSDMGEQDSGTQALIKALAYRSEMTL
>Mature_298_residues
MLSANTTTIGFIGTGVMGKSMAKNLLNAGYSIRIFTRTKSKADDLLNIGAKWDKDIPTLAKQCDVIITMVGYPSDVEEVY
LGEQGLIKNCNKGSYLIDMTTSKPSLAEEIFSVSEKHGLHVLDAPVSGGDIGAESGTLAIMTGGERSVYDYVLPIFQVLG
ENISYQGPAGTGQHTKMSNQIAIASNMIGVCESLLYAKKAGLDPKKVLATISTGAAASFSLSKLGARMLENDFNPGFYVK
HFIKDMRIALESSQAFGLDTPGLKLAIDLYEQLSDMGEQDSGTQALIKALAYRSEMTL

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=257, Percent_Identity=33.852140077821, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI40556376, Length=284, Percent_Identity=27.112676056338, Blast_Score=132, Evalue=5e-31,
Organism=Escherichia coli, GI1786719, Length=282, Percent_Identity=39.3617021276596, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI145693186, Length=265, Percent_Identity=36.9811320754717, Blast_Score=187, Evalue=5e-49,
Organism=Escherichia coli, GI1790315, Length=298, Percent_Identity=27.1812080536913, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI1789092, Length=283, Percent_Identity=24.7349823321555, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17557316, Length=273, Percent_Identity=26.7399267399267, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321695, Length=236, Percent_Identity=25, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24655230, Length=278, Percent_Identity=32.7338129496403, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI19922568, Length=278, Percent_Identity=32.7338129496403, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI28574115, Length=279, Percent_Identity=25.089605734767, Blast_Score=100, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31932; Mature: 31932

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSANTTTIGFIGTGVMGKSMAKNLLNAGYSIRIFTRTKSKADDLLNIGAKWDKDIPTLA
CCCCCCEEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCCCCCHHHHH
KQCDVIITMVGYPSDVEEVYLGEQGLIKNCNKGSYLIDMTTSKPSLAEEIFSVSEKHGLH
HHCCEEEEEECCCCCHHHHHCCCCHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCE
VLDAPVSGGDIGAESGTLAIMTGGERSVYDYVLPIFQVLGENISYQGPAGTGQHTKMSNQ
EEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
IAIASNMIGVCESLLYAKKAGLDPKKVLATISTGAAASFSLSKLGARMLENDFNPGFYVK
EEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
HFIKDMRIALESSQAFGLDTPGLKLAIDLYEQLSDMGEQDSGTQALIKALAYRSEMTL
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLSANTTTIGFIGTGVMGKSMAKNLLNAGYSIRIFTRTKSKADDLLNIGAKWDKDIPTLA
CCCCCCEEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCCCCCHHHHH
KQCDVIITMVGYPSDVEEVYLGEQGLIKNCNKGSYLIDMTTSKPSLAEEIFSVSEKHGLH
HHCCEEEEEECCCCCHHHHHCCCCHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCE
VLDAPVSGGDIGAESGTLAIMTGGERSVYDYVLPIFQVLGENISYQGPAGTGQHTKMSNQ
EEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
IAIASNMIGVCESLLYAKKAGLDPKKVLATISTGAAASFSLSKLGARMLENDFNPGFYVK
EEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
HFIKDMRIALESSQAFGLDTPGLKLAIDLYEQLSDMGEQDSGTQALIKALAYRSEMTL
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]