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The map label for this gene is cysK [H]

Identifier: 23097539

GI number: 23097539

Start: 86428

End: 87354

Strand: Direct

Name: cysK [H]

Synonym: OB0084

Alternate gene names: 23097539

Gene position: 86428-87354 (Clockwise)

Preceding gene: 23097538

Following gene: 23097540

Centisome position: 2.38

GC content: 41.53

Gene sequence:

>927_bases
ATGAGGGTCTCTGATAATATTACAGGTTTAATTGGCGGAACACCGATTGTTAAATTACACAATGTTACAGATGAAGGTAG
TGCAGATGTATACGCAAAGGTAGAGTTTATGAATCCTGGTAGCTCTGTGAAAGACCGTATTGCTATCGCGATGATTGAAG
CAGCAGAACAAGGTGGAAAGCTAAAAGAAGGCGATACTATTATTGAGCCGACTAGTGGAAATACTGGTATTGGCCTTGCA
ATGGTAGCAGCTGCAAAAGGATACCGTGCAGTTCTAGTAATGCCAGATACGATGAGTCAGGAACGCAGGAATCTCCTTCG
TGCTTATGGTGCTGAACTTGTACTGACACCTGGTGCTGAAGGGATGAAAGGTGCAATTGCGAAAGCGGTAGAATTGCAAG
AAGAGAATGATTACTTTATGCCGCAACAATTTAGCAACCCTGCAAATCCTGATATTCACGAAAAAACGACTGGTAAAGAA
ATTGTGGAGCAGATGAAAGATGGTCTTGATGGATTCGTATCTGGTATTGGTACTGGTGGAACAATTACCGGTGCAGGTAA
GGTGCTAAAAAATCATTTTCAAGATATTAAATTATACGCAGTAGAGCCGGCTGATTCTCCAATACTTTCAGGAGGAAGTC
CTGGGCCGCATAAAATACAAGGAATTGGAGCCGGCTTTAAACCGGATGTGTTAGACACCGCAATCTATGATGAAGTTTTA
ACAATTAGTAATGAGGAAGCATATGAAGCAGCTAGAAAAGTTGCAACGACCAATGGCATATTGGGAGGTATTTCTTCCGG
TGCTGCAGTTGCTGCTGCAGTTAAAGTTGCTAAACAATTAGGCGCAGGTAAGAAAGTTCTTGCTATATTACCAGATAATG
GTGAGCGCTATCTATCAACACCGTTATATCAATTTGATAAAGAGTAA

Upstream 100 bases:

>100_bases
AATATGAAATTATCAGACAAATTAGTTGACATTTTAGGATAAAGGAACTACATTAGATGTAAATCTTATAAAAATACTTG
GAATTAGAGGAGGAAGTCAG

Downstream 100 bases:

>100_bases
AAGTTATGATGGGGCTGGCCAAATTGGTCAGCTCTTTTTTGTTTATGGTGGTGCTTCCGTTCTGTGAGTAGTTATTGTAC
GATGAAAGAATAGATTATGT

Product: cysteine synthase A

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; Superoxide-inducible protein 11; SOI11 [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MRVSDNITGLIGGTPIVKLHNVTDEGSADVYAKVEFMNPGSSVKDRIAIAMIEAAEQGGKLKEGDTIIEPTSGNTGIGLA
MVAAAKGYRAVLVMPDTMSQERRNLLRAYGAELVLTPGAEGMKGAIAKAVELQEENDYFMPQQFSNPANPDIHEKTTGKE
IVEQMKDGLDGFVSGIGTGGTITGAGKVLKNHFQDIKLYAVEPADSPILSGGSPGPHKIQGIGAGFKPDVLDTAIYDEVL
TISNEEAYEAARKVATTNGILGGISSGAAVAAAVKVAKQLGAGKKVLAILPDNGERYLSTPLYQFDKE

Sequences:

>Translated_308_residues
MRVSDNITGLIGGTPIVKLHNVTDEGSADVYAKVEFMNPGSSVKDRIAIAMIEAAEQGGKLKEGDTIIEPTSGNTGIGLA
MVAAAKGYRAVLVMPDTMSQERRNLLRAYGAELVLTPGAEGMKGAIAKAVELQEENDYFMPQQFSNPANPDIHEKTTGKE
IVEQMKDGLDGFVSGIGTGGTITGAGKVLKNHFQDIKLYAVEPADSPILSGGSPGPHKIQGIGAGFKPDVLDTAIYDEVL
TISNEEAYEAARKVATTNGILGGISSGAAVAAAVKVAKQLGAGKKVLAILPDNGERYLSTPLYQFDKE
>Mature_308_residues
MRVSDNITGLIGGTPIVKLHNVTDEGSADVYAKVEFMNPGSSVKDRIAIAMIEAAEQGGKLKEGDTIIEPTSGNTGIGLA
MVAAAKGYRAVLVMPDTMSQERRNLLRAYGAELVLTPGAEGMKGAIAKAVELQEENDYFMPQQFSNPANPDIHEKTTGKE
IVEQMKDGLDGFVSGIGTGGTITGAGKVLKNHFQDIKLYAVEPADSPILSGGSPGPHKIQGIGAGFKPDVLDTAIYDEVL
TISNEEAYEAARKVATTNGILGGISSGAAVAAAVKVAKQLGAGKKVLAILPDNGERYLSTPLYQFDKE

Specific function: Catalyzes the conversion of O-acetylserine to cysteine. Also acts as a sensor of cysteine availability in the signal transduction pathway modulating CymR activity. When cysteine is present, the pool of O-acetylserine (OAS) is low, which leads to the forma

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=310, Percent_Identity=40.6451612903226, Blast_Score=224, Evalue=9e-59,
Organism=Homo sapiens, GI295821200, Length=310, Percent_Identity=40.6451612903226, Blast_Score=224, Evalue=9e-59,
Organism=Homo sapiens, GI4557415, Length=310, Percent_Identity=40.6451612903226, Blast_Score=224, Evalue=9e-59,
Organism=Escherichia coli, GI1788754, Length=304, Percent_Identity=53.9473684210526, Blast_Score=272, Evalue=2e-74,
Organism=Escherichia coli, GI2367138, Length=293, Percent_Identity=43.0034129692833, Blast_Score=231, Evalue=6e-62,
Organism=Caenorhabditis elegans, GI115535073, Length=309, Percent_Identity=52.1035598705502, Blast_Score=273, Evalue=6e-74,
Organism=Caenorhabditis elegans, GI17535051, Length=298, Percent_Identity=47.6510067114094, Blast_Score=270, Evalue=9e-73,
Organism=Caenorhabditis elegans, GI17562970, Length=297, Percent_Identity=46.4646464646465, Blast_Score=255, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI32566674, Length=292, Percent_Identity=46.5753424657534, Blast_Score=250, Evalue=8e-67,
Organism=Caenorhabditis elegans, GI17561720, Length=297, Percent_Identity=46.1279461279461, Blast_Score=232, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI32566672, Length=213, Percent_Identity=46.4788732394366, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17534315, Length=314, Percent_Identity=39.171974522293, Blast_Score=166, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI25147552, Length=315, Percent_Identity=37.4603174603175, Blast_Score=163, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI17561716, Length=118, Percent_Identity=44.9152542372881, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71996324, Length=332, Percent_Identity=26.2048192771084, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6321594, Length=315, Percent_Identity=36.8253968253968, Blast_Score=189, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6321449, Length=340, Percent_Identity=32.3529411764706, Blast_Score=136, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24643623, Length=309, Percent_Identity=38.8349514563107, Blast_Score=200, Evalue=8e-52,
Organism=Drosophila melanogaster, GI20129101, Length=309, Percent_Identity=38.8349514563107, Blast_Score=200, Evalue=8e-52,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32275; Mature: 32275

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSDNITGLIGGTPIVKLHNVTDEGSADVYAKVEFMNPGSSVKDRIAIAMIEAAEQGGK
CCCCCCCCEEECCCCEEEEECCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCC
LKEGDTIIEPTSGNTGIGLAMVAAAKGYRAVLVMPDTMSQERRNLLRAYGAELVLTPGAE
CCCCCEEEECCCCCCCCHHHHEECCCCCEEEEEECCCHHHHHHHHHHHCCCCEEECCCCC
GMKGAIAKAVELQEENDYFMPQQFSNPANPDIHEKTTGKEIVEQMKDGLDGFVSGIGTGG
CHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
TITGAGKVLKNHFQDIKLYAVEPADSPILSGGSPGPHKIQGIGAGFKPDVLDTAIYDEVL
EECHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHH
TISNEEAYEAARKVATTNGILGGISSGAAVAAAVKVAKQLGAGKKVLAILPDNGERYLST
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
PLYQFDKE
CHHHCCCC
>Mature Secondary Structure
MRVSDNITGLIGGTPIVKLHNVTDEGSADVYAKVEFMNPGSSVKDRIAIAMIEAAEQGGK
CCCCCCCCEEECCCCEEEEECCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCC
LKEGDTIIEPTSGNTGIGLAMVAAAKGYRAVLVMPDTMSQERRNLLRAYGAELVLTPGAE
CCCCCEEEECCCCCCCCHHHHEECCCCCEEEEEECCCHHHHHHHHHHHCCCCEEECCCCC
GMKGAIAKAVELQEENDYFMPQQFSNPANPDIHEKTTGKEIVEQMKDGLDGFVSGIGTGG
CHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
TITGAGKVLKNHFQDIKLYAVEPADSPILSGGSPGPHKIQGIGAGFKPDVLDTAIYDEVL
EECHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHH
TISNEEAYEAARKVATTNGILGGISSGAAVAAAVKVAKQLGAGKKVLAILPDNGERYLST
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
PLYQFDKE
CHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 8755892; 9298659 [H]