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The map label for this gene is rasP [H]

Identifier: 229918662

GI number: 229918662

Start: 2906145

End: 2907395

Strand: Direct

Name: rasP [H]

Synonym: EAT1b_2949

Alternate gene names: 229918662

Gene position: 2906145-2907395 (Clockwise)

Preceding gene: 229918661

Following gene: 229918663

Centisome position: 96.87

GC content: 50.52

Gene sequence:

>1251_bases
ATGACGACTTTTATCGCCATCGTTTTGATGTTTGGCGTTCTCATTTCGGTACACGAATGGGGACACCTCGTCATGGCGAA
ACGGGCAGGCATTCTCTGTCACGAGTTTGCAATCGGATTCGGTCCAAAAATTGTTTCATTCCGTAAAAATGAAACGCTCT
ATACCATTCGTCTTTTACCAATCGGTGGCTACGTCAAAATGGCCGGAGAAGATTTTGAACCGATTGAAGTCCGTGCGGGT
CAGCATGTCGGACTCCGGCTCACCGAACAGGGGACGGTCGATCGTGTCTACTTCCAACCGGACACGGCTACAGATGTCCA
TGTCGTAGGAACCGTCGATAAGTTCGATTCGACACATGAGTTGAAAGTGCAACTTCTCATCGATGAGGAAGTGCGTGTCT
ATTCATTAGAGCGAGACACACTCCTTGTCGACCAAGGGATGGAAATTCAAATCGCACCGTATGATCGAACATTTGGAGCC
CAGTCGGTTTGGAAACGTGTCTTGGCCATCGCAGCCGGTCCGGCGATGAACTTCGTCTTGGCGTTCATCTTGCTCATTAT
TGTGGGGCTCGTTCAAGGAACCCCGACGAACGATGGTCAAATCGGTACGGTTCAACCGGATTCAGCCGCTGATGAGGCTG
GTTTGATGAGCGGGGATGAAATCGTATCGATTGAAGGTGAGCCGATTACCGATTGGCTCGACTTACGATCTGCTTTAGAA
GACCGGGCGGACACCCCTACGGAAGTCACGTATGTCCGTGATGGGGAGGAAGCAACTGTCACCCTCACACCACAAGCAGT
CGAACAAAACGGAGAGACGGTCGGGATTCTTGGTGTCACGAACGCACTCGAGCGATCACCGGTCAAAGCCGTGACGACGG
GAGCGGAAACGACATGGACGATGTCGACGCTCATCTTCTCTGCGGTCGGTGATTTGGTGACAGGACAAGTCGGTGTCGAT
CAACTAGCAGGTCCGGTCGGGATTGTACGGATGACAGATGAAGTAGCGGCGAGCGGTTTGATCATGCTATTGAACTGGAC
AGCGCTTCTCTCCGTCAACCTTGCTATCTTCAACTTGCTCCCATTGCCAGCGTTAGACGGAGGACGTTTGATTTTCCTCT
TGTTCGAAGCGGTACGCGGACGTCCAATCGATCCGAAAAAAGAAGGATTCGTCCACTTTATCGGATTCGCACTCTTGATG
ATTTTAATGTTGATTGTCACTTGGAACGACATTCAATCATTCTTTAAATAA

Upstream 100 bases:

>100_bases
ACATGAGGTCATCGCCGAGCCGACGCTCGCGCAAATTCTAGAAGTTGACCGAGCAGTACGTCAACAGATTCAGCAAGGAA
TTGAAGGTGGGGAATAAATC

Downstream 100 bases:

>100_bases
GCTGTTTGTAGAGAAGGGAAACGTTGAACTGTATGAAACAATCTAGATTGCTCATGCCTACATTGAGAGAAGTTCCGGCT
GACGCGGAAGCGGTCAGCCA

Product: membrane-associated zinc metalloprotease

Products: NA

Alternate protein names: Regulating alternative sigma factor protease; Regulating anti-sigma-W factor activity protease [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MTTFIAIVLMFGVLISVHEWGHLVMAKRAGILCHEFAIGFGPKIVSFRKNETLYTIRLLPIGGYVKMAGEDFEPIEVRAG
QHVGLRLTEQGTVDRVYFQPDTATDVHVVGTVDKFDSTHELKVQLLIDEEVRVYSLERDTLLVDQGMEIQIAPYDRTFGA
QSVWKRVLAIAAGPAMNFVLAFILLIIVGLVQGTPTNDGQIGTVQPDSAADEAGLMSGDEIVSIEGEPITDWLDLRSALE
DRADTPTEVTYVRDGEEATVTLTPQAVEQNGETVGILGVTNALERSPVKAVTTGAETTWTMSTLIFSAVGDLVTGQVGVD
QLAGPVGIVRMTDEVAASGLIMLLNWTALLSVNLAIFNLLPLPALDGGRLIFLLFEAVRGRPIDPKKEGFVHFIGFALLM
ILMLIVTWNDIQSFFK

Sequences:

>Translated_416_residues
MTTFIAIVLMFGVLISVHEWGHLVMAKRAGILCHEFAIGFGPKIVSFRKNETLYTIRLLPIGGYVKMAGEDFEPIEVRAG
QHVGLRLTEQGTVDRVYFQPDTATDVHVVGTVDKFDSTHELKVQLLIDEEVRVYSLERDTLLVDQGMEIQIAPYDRTFGA
QSVWKRVLAIAAGPAMNFVLAFILLIIVGLVQGTPTNDGQIGTVQPDSAADEAGLMSGDEIVSIEGEPITDWLDLRSALE
DRADTPTEVTYVRDGEEATVTLTPQAVEQNGETVGILGVTNALERSPVKAVTTGAETTWTMSTLIFSAVGDLVTGQVGVD
QLAGPVGIVRMTDEVAASGLIMLLNWTALLSVNLAIFNLLPLPALDGGRLIFLLFEAVRGRPIDPKKEGFVHFIGFALLM
ILMLIVTWNDIQSFFK
>Mature_415_residues
TTFIAIVLMFGVLISVHEWGHLVMAKRAGILCHEFAIGFGPKIVSFRKNETLYTIRLLPIGGYVKMAGEDFEPIEVRAGQ
HVGLRLTEQGTVDRVYFQPDTATDVHVVGTVDKFDSTHELKVQLLIDEEVRVYSLERDTLLVDQGMEIQIAPYDRTFGAQ
SVWKRVLAIAAGPAMNFVLAFILLIIVGLVQGTPTNDGQIGTVQPDSAADEAGLMSGDEIVSIEGEPITDWLDLRSALED
RADTPTEVTYVRDGEEATVTLTPQAVEQNGETVGILGVTNALERSPVKAVTTGAETTWTMSTLIFSAVGDLVTGQVGVDQ
LAGPVGIVRMTDEVAASGLIMLLNWTALLSVNLAIFNLLPLPALDGGRLIFLLFEAVRGRPIDPKKEGFVHFIGFALLMI
LMLIVTWNDIQSFFK

Specific function: Is responsible for Site-2 cleavage of the RsiW anti- sigma factor. This results, after a third proteolytic step catalyzed by the ClpXP protease, in the release of SigW and the transcription activation of the genes under the control of the sigma-W factor [

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain [H]

Homologues:

Organism=Escherichia coli, GI1786373, Length=219, Percent_Identity=34.703196347032, Blast_Score=139, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR004387
- InterPro:   IPR008915 [H]

Pfam domain/function: PF00595 PDZ; PF02163 Peptidase_M50 [H]

EC number: NA

Molecular weight: Translated: 45326; Mature: 45195

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFIAIVLMFGVLISVHEWGHLVMAKRAGILCHEFAIGFGPKIVSFRKNETLYTIRLLP
CHHHHHHHHHHHHHHHHHHCCCEEEHHHCCCEEEHHHHCCCCEEEEEECCCEEEEEEEEE
IGGYVKMAGEDFEPIEVRAGQHVGLRLTEQGTVDRVYFQPDTATDVHVVGTVDKFDSTHE
ECCEEEECCCCCCCEEEECCCEEEEEEECCCCEEEEEECCCCCCCEEEEEEHHHCCCCCE
LKVQLLIDEEVRVYSLERDTLLVDQGMEIQIAPYDRTFGAQSVWKRVLAIAAGPAMNFVL
EEEEEEEECCEEEEEECCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHH
AFILLIIVGLVQGTPTNDGQIGTVQPDSAADEAGLMSGDEIVSIEGEPITDWLDLRSALE
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHH
DRADTPTEVTYVRDGEEATVTLTPQAVEQNGETVGILGVTNALERSPVKAVTTGAETTWT
HCCCCCCEEEEEECCCCEEEEECHHHHHCCCCEEEEEEECHHHHCCCCEEEECCCCCHHH
MSTLIFSAVGDLVTGQVGVDQLAGPVGIVRMTDEVAASGLIMLLNWTALLSVNLAIFNLL
HHHHHHHHHHHHHCCCCCHHHHCCCEEEEEECHHHHHCCEEEEEEHHHHHHHHHHHHHHC
PLPALDGGRLIFLLFEAVRGRPIDPKKEGFVHFIGFALLMILMLIVTWNDIQSFFK
CCCCCCCCHHHEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
TTFIAIVLMFGVLISVHEWGHLVMAKRAGILCHEFAIGFGPKIVSFRKNETLYTIRLLP
HHHHHHHHHHHHHHHHHHCCCEEEHHHCCCEEEHHHHCCCCEEEEEECCCEEEEEEEEE
IGGYVKMAGEDFEPIEVRAGQHVGLRLTEQGTVDRVYFQPDTATDVHVVGTVDKFDSTHE
ECCEEEECCCCCCCEEEECCCEEEEEEECCCCEEEEEECCCCCCCEEEEEEHHHCCCCCE
LKVQLLIDEEVRVYSLERDTLLVDQGMEIQIAPYDRTFGAQSVWKRVLAIAAGPAMNFVL
EEEEEEEECCEEEEEECCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHH
AFILLIIVGLVQGTPTNDGQIGTVQPDSAADEAGLMSGDEIVSIEGEPITDWLDLRSALE
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHH
DRADTPTEVTYVRDGEEATVTLTPQAVEQNGETVGILGVTNALERSPVKAVTTGAETTWT
HCCCCCCEEEEEECCCCEEEEECHHHHHCCCCEEEEEEECHHHHCCCCEEEECCCCCHHH
MSTLIFSAVGDLVTGQVGVDQLAGPVGIVRMTDEVAASGLIMLLNWTALLSVNLAIFNLL
HHHHHHHHHHHHHCCCCCHHHHCCCEEEEEECHHHHHCCEEEEEEHHHHHHHHHHHHHHC
PLPALDGGRLIFLLFEAVRGRPIDPKKEGFVHFIGFALLMILMLIVTWNDIQSFFK
CCCCCCCCHHHEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]