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The map label for this gene is proS [H]

Identifier: 229918663

GI number: 229918663

Start: 2907419

End: 2909128

Strand: Direct

Name: proS [H]

Synonym: EAT1b_2950

Alternate gene names: 229918663

Gene position: 2907419-2909128 (Clockwise)

Preceding gene: 229918662

Following gene: 229918664

Centisome position: 96.92

GC content: 49.88

Gene sequence:

>1710_bases
TTGAACTGTATGAAACAATCTAGATTGCTCATGCCTACATTGAGAGAAGTTCCGGCTGACGCGGAAGCGGTCAGCCATCA
ACTCCTCGTCCGTGGAGGATTCATCCGTCAAAATGCGGCAGGAATTTATTCTTATCTTCCACTCGGACATAAGGTGTTAC
ACAACATCCAAACAATCATCCGTGAAGAGATGAATCGTGCCGGTGCACAAGAGTTATTGATGCCTGCCATTCAACCGGCA
GAACTTTGGGAAGAGACAGGACGTTGGGGCATTTACGGTCCTGAGTTGATGCGCTTGACGGACCGTCATGACCGTCGTTT
CGCACTCGGTGCGACACACGAAGAATTGATCACATCGATCGTCCGAGACGAGCTCAACTCGTATAAAAAACTTCCGATGA
ACTTGTACCAGATTCAAACGAAGTATCGTGATGAGCGTCGTCCACGTTTCGGGTTGCTCCGCGGACGTGAGTTCTTGATG
AAAGACGCCTACTCATTCCACGCGACACAAGAAGACTTAGATGAAGAATATACAAACATGTATAATGCGTACTCACGCAT
CTTCGACCGCACAGGACTGAAGTATCGTCCGGTCGTCGCGGACTCTGGGGCAATCGGCGGGAAAGATACGCATGAATTCC
AAGCGCTTGCGGATATCGGGGAAGACACGATTGTCTACACGGACACGTCGAACTATGCGGCGAACATCGAGATGGCCGAA
ACGCTCGACCGTTATGAGATGCAGCAAGCTGACGTTCGTCCACTCGAAAAAGTGGACACGGAAGAGAATCGTACGATTGA
AGATGTGGCAGCATTCTTGAACGTGGATCCGAAAACGACAATCAAATCTGTCTTGTTCAACGTGGATGGCGAAACCGTCA
TGGCAATCGTTCGTGGCGACCACGAAGCGAACGAAGTCAAAGTGAAGAACGCACTCGGTGGACTCGACATTCAACTTGAA
GAAGAAGCGAAAATCGTTGAGTTGTTCAACTGTGCGCCAGGTACACTCGGACCGATTAACGCTCCGGTCAAAGTCGTTGC
CGACTATGCGGTCAAGTACTTAGTGGATGCAGTCTGTGGTGCCAACGAGGCGAACACACACTACACGGGTGTAAACGCTG
CGCGCGACCTTGCCGAAATGACATACCACGATTTACGTTTCGTTGTCGAAGGCGATTTGGCACCGGATGAGTCCGGTCCG
GTTCGTTTTGCTCGTGGGATTGAAGTGGGGCAAGTATTTAAACTCGGAACACGATATTCAGAAGCGATGGGTGCGACGTT
CCTCAACGAAGAGGGTCGTGCCGAACCACTCATCATGGGTTGCTACGGCATCGGAGTGTCACGCACACTTTCAGCCATCG
TCGAACAACATCACGACGACCGTGGAATCGTTTGGCCACGTAACGTGGCGCCGTTCGATCTCCATTTGATCGCAATCAAT
GCAAAAGATGAGACGCAAGTCGAGTTGGCGAACCGTCTTTATGAAGAGCTTGGTCAAACATACGATGTCTTGTTCGACGA
CCGAAAAGAACGCGCGGGCGTCAAGTTTGCAGATGCAGATTTGATTGGACTTCCGATTCGCGTGAACGTCGGGAAAAAAG
CCGGCGAAGGCATCGTTGAAGTGAAAGTACGTGCAACAGGTGAAGTGATTGAAACATCAATCGATGAATTGAAAGAAGTC
ATCGCTTCAAAATTTAGCGAAGTTCATTAA

Upstream 100 bases:

>100_bases
ACTTTATCGGATTCGCACTCTTGATGATTTTAATGTTGATTGTCACTTGGAACGACATTCAATCATTCTTTAAATAAGCT
GTTTGTAGAGAAGGGAAACG

Downstream 100 bases:

>100_bases
TGGATGAAGGTGACAGCGGAAGCGGTCACCTTCATTTGTCGAATTGGCATAAAGGGGGAAAGACGGACCCGTGGAAGCAA
ATCAAAAGATGCAATTATTA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 569; Mature: 569

Protein sequence:

>569_residues
MNCMKQSRLLMPTLREVPADAEAVSHQLLVRGGFIRQNAAGIYSYLPLGHKVLHNIQTIIREEMNRAGAQELLMPAIQPA
ELWEETGRWGIYGPELMRLTDRHDRRFALGATHEELITSIVRDELNSYKKLPMNLYQIQTKYRDERRPRFGLLRGREFLM
KDAYSFHATQEDLDEEYTNMYNAYSRIFDRTGLKYRPVVADSGAIGGKDTHEFQALADIGEDTIVYTDTSNYAANIEMAE
TLDRYEMQQADVRPLEKVDTEENRTIEDVAAFLNVDPKTTIKSVLFNVDGETVMAIVRGDHEANEVKVKNALGGLDIQLE
EEAKIVELFNCAPGTLGPINAPVKVVADYAVKYLVDAVCGANEANTHYTGVNAARDLAEMTYHDLRFVVEGDLAPDESGP
VRFARGIEVGQVFKLGTRYSEAMGATFLNEEGRAEPLIMGCYGIGVSRTLSAIVEQHHDDRGIVWPRNVAPFDLHLIAIN
AKDETQVELANRLYEELGQTYDVLFDDRKERAGVKFADADLIGLPIRVNVGKKAGEGIVEVKVRATGEVIETSIDELKEV
IASKFSEVH

Sequences:

>Translated_569_residues
MNCMKQSRLLMPTLREVPADAEAVSHQLLVRGGFIRQNAAGIYSYLPLGHKVLHNIQTIIREEMNRAGAQELLMPAIQPA
ELWEETGRWGIYGPELMRLTDRHDRRFALGATHEELITSIVRDELNSYKKLPMNLYQIQTKYRDERRPRFGLLRGREFLM
KDAYSFHATQEDLDEEYTNMYNAYSRIFDRTGLKYRPVVADSGAIGGKDTHEFQALADIGEDTIVYTDTSNYAANIEMAE
TLDRYEMQQADVRPLEKVDTEENRTIEDVAAFLNVDPKTTIKSVLFNVDGETVMAIVRGDHEANEVKVKNALGGLDIQLE
EEAKIVELFNCAPGTLGPINAPVKVVADYAVKYLVDAVCGANEANTHYTGVNAARDLAEMTYHDLRFVVEGDLAPDESGP
VRFARGIEVGQVFKLGTRYSEAMGATFLNEEGRAEPLIMGCYGIGVSRTLSAIVEQHHDDRGIVWPRNVAPFDLHLIAIN
AKDETQVELANRLYEELGQTYDVLFDDRKERAGVKFADADLIGLPIRVNVGKKAGEGIVEVKVRATGEVIETSIDELKEV
IASKFSEVH
>Mature_569_residues
MNCMKQSRLLMPTLREVPADAEAVSHQLLVRGGFIRQNAAGIYSYLPLGHKVLHNIQTIIREEMNRAGAQELLMPAIQPA
ELWEETGRWGIYGPELMRLTDRHDRRFALGATHEELITSIVRDELNSYKKLPMNLYQIQTKYRDERRPRFGLLRGREFLM
KDAYSFHATQEDLDEEYTNMYNAYSRIFDRTGLKYRPVVADSGAIGGKDTHEFQALADIGEDTIVYTDTSNYAANIEMAE
TLDRYEMQQADVRPLEKVDTEENRTIEDVAAFLNVDPKTTIKSVLFNVDGETVMAIVRGDHEANEVKVKNALGGLDIQLE
EEAKIVELFNCAPGTLGPINAPVKVVADYAVKYLVDAVCGANEANTHYTGVNAARDLAEMTYHDLRFVVEGDLAPDESGP
VRFARGIEVGQVFKLGTRYSEAMGATFLNEEGRAEPLIMGCYGIGVSRTLSAIVEQHHDDRGIVWPRNVAPFDLHLIAIN
AKDETQVELANRLYEELGQTYDVLFDDRKERAGVKFADADLIGLPIRVNVGKKAGEGIVEVKVRATGEVIETSIDELKEV
IASKFSEVH

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=295, Percent_Identity=37.9661016949153, Blast_Score=208, Evalue=9e-54,
Organism=Escherichia coli, GI1786392, Length=555, Percent_Identity=50.4504504504504, Blast_Score=556, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI115532348, Length=219, Percent_Identity=38.8127853881278, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI193203271, Length=89, Percent_Identity=39.3258426966292, Blast_Score=82, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6320931, Length=548, Percent_Identity=28.8321167883212, Blast_Score=221, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24656200, Length=277, Percent_Identity=31.4079422382672, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63775; Mature: 63775

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNCMKQSRLLMPTLREVPADAEAVSHQLLVRGGFIRQNAAGIYSYLPLGHKVLHNIQTII
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHH
REEMNRAGAQELLMPAIQPAELWEETGRWGIYGPELMRLTDRHDRRFALGATHEELITSI
HHHHHHCCHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEECCCHHHHHHHH
VRDELNSYKKLPMNLYQIQTKYRDERRPRFGLLRGREFLMKDAYSFHATQEDLDEEYTNM
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
YNAYSRIFDRTGLKYRPVVADSGAIGGKDTHEFQALADIGEDTIVYTDTSNYAANIEMAE
HHHHHHHHHHCCCEEECEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCEECCHHHH
TLDRYEMQQADVRPLEKVDTEENRTIEDVAAFLNVDPKTTIKSVLFNVDGETVMAIVRGD
HHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECC
HEANEVKVKNALGGLDIQLEEEAKIVELFNCAPGTLGPINAPVKVVADYAVKYLVDAVCG
CCCCCEEEECCCCCCCEEECCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
ANEANTHYTGVNAARDLAEMTYHDLRFVVEGDLAPDESGPVRFARGIEVGQVFKLGTRYS
CCCCCCEECCCHHHHHHHHHHHHHEEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHH
EAMGATFLNEEGRAEPLIMGCYGIGVSRTLSAIVEQHHDDRGIVWPRNVAPFDLHLIAIN
HHHCCHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEC
AKDETQVELANRLYEELGQTYDVLFDDRKERAGVKFADADLIGLPIRVNVGKKAGEGIVE
CCCCHHHHHHHHHHHHHCCHHHHHHCCCHHHCCCEEECCCEEECEEEEECCCCCCCCEEE
VKVRATGEVIETSIDELKEVIASKFSEVH
EEEECCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNCMKQSRLLMPTLREVPADAEAVSHQLLVRGGFIRQNAAGIYSYLPLGHKVLHNIQTII
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHH
REEMNRAGAQELLMPAIQPAELWEETGRWGIYGPELMRLTDRHDRRFALGATHEELITSI
HHHHHHCCHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEECCCHHHHHHHH
VRDELNSYKKLPMNLYQIQTKYRDERRPRFGLLRGREFLMKDAYSFHATQEDLDEEYTNM
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
YNAYSRIFDRTGLKYRPVVADSGAIGGKDTHEFQALADIGEDTIVYTDTSNYAANIEMAE
HHHHHHHHHHCCCEEECEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCEECCHHHH
TLDRYEMQQADVRPLEKVDTEENRTIEDVAAFLNVDPKTTIKSVLFNVDGETVMAIVRGD
HHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECC
HEANEVKVKNALGGLDIQLEEEAKIVELFNCAPGTLGPINAPVKVVADYAVKYLVDAVCG
CCCCCEEEECCCCCCCEEECCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
ANEANTHYTGVNAARDLAEMTYHDLRFVVEGDLAPDESGPVRFARGIEVGQVFKLGTRYS
CCCCCCEECCCHHHHHHHHHHHHHEEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHH
EAMGATFLNEEGRAEPLIMGCYGIGVSRTLSAIVEQHHDDRGIVWPRNVAPFDLHLIAIN
HHHCCHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEC
AKDETQVELANRLYEELGQTYDVLFDDRKERAGVKFADADLIGLPIRVNVGKKAGEGIVE
CCCCHHHHHHHHHHHHHCCHHHHHHCCCHHHCCCEEECCCEEECEEEEECCCCCCCCEEE
VKVRATGEVIETSIDELKEVIASKFSEVH
EEEECCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA